pycom08g20820

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Reverse (-)
20471288 .. 20474981
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
ATGGACACCAATCGCTGTAATGTGAGGTATTTTTCATCTCCACATGGAATTTACAAGGAGAATTCTTGCCCGAGGATCATGGAGATCATCCAATTACATTCGTTCATTGTAAATAGTACGATAAACAATCATTTATATAAGATACCTTCTCCTCCCGGTTCTCCTAAAAACAGAAGCTCCTCCGTCCTCCGTCCTCCGTCCGCCGTCTCTCTCACAATCACAGACACAGCAAAAAATGAAAGCTGCGAAGCCATGGATAAACGCTTTCAGAGCAAAATCAAAGCAAGCCACGCTGCTTTGGCTCGAGGGCTTCTGAGAGGCCTGCTGCCTTCACCGCGTCCTCATCCTCTGCCACCGGTCGAGAAAGCTTATGATACGAACAGGACAGTGTTTTCTATTAAACGGCTTCATTTTCTTAGGAAGGTAAATGTCGATGCTGTCAAGCTTTTAGATGCTTATATGGTCGCCTTAGTTTTCATGTCCCAGTATTACCTCTACATAGACATCATTATAATTATCCTTAACTCAGTCATCATCCCAACTCTACACTGGATATTACCTGATAGATGCCCAGAATATAGTTCTCAACAACTCTGCTCATTTGGTGGTATATTGAAAATTTATTCCTTCTTGCGGCAGGGACTTGTACAACTGTTTTATGTAACTTGGTTCTACCCACTATACGTATTCAGCATTATCCTGAGCAACATCTGGTACAATGACATTGCTAAGTATGGATTTTCTTCAATGGGGAGGTCCACGCCAACCACATTGGATCCTGTCAGACAAAATGAGGCATTGGTCTTACAAAATAATGTTGGTCCGGAAAGGCCTGCTGGCCTGGGAGGGGTCATGATTGGTATAGGAGAGCAGGTGTATTCAGTACTTCTTTTGAGTTGTTTCTTCCTAGAGGTACAAATGGAATTTTTCTGA

Protein Analysis

311

Amino Acids

35.19

Weight (kDa)

9.23

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
AarI CACCTGC 1 cut(s) 862
Acc36I ACCTGC 1 cut(s) 862
AccII CGCG 1 cut(s) 337
AccIII TCCGGA 1 cut(s) 823
AciI CCGC 3 cut(s) 201, 335, 634
AclWI GGATC 3 cut(s) 83, 770, 783
AcsI RAATTY 4 cut(s) 48, 61, 618, 923
AfaI GTAC 5 cut(s) 118, 648, 716, 885, 915
AfiI CCNNNNNNNGG 2 cut(s) 633, 847
AgeI ACCGGT 1 cut(s) 355
AgsI TTSAA 2 cut(s) 616, 747
AjnI CCWGG 1 cut(s) 840
AluBI AGCT 4 cut(s) 177, 243, 368, 445
AluI AGCT 4 cut(s) 177, 243, 368, 445
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 3 cut(s) 83, 770, 783
Ama87I CYCGRG 2 cut(s) 70, 303
Aor13HI TCCGGA 1 cut(s) 823
AoxI GGCC 3 cut(s) 319, 830, 838
ApeKI GCWGC 3 cut(s) 243, 293, 325
ApoI RAATTY 4 cut(s) 48, 61, 618, 923
AsiGI ACCGGT 1 cut(s) 355
AspS9I GGNCC 2 cut(s) 756, 821
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 324
AvaI CYCGRG 2 cut(s) 70, 303
AvaII GGWCC 2 cut(s) 756, 821
BamHI GGATCC 1 cut(s) 775
BbvI GCAGC 3 cut(s) 230, 280, 312
BceAI ACGGC 2 cut(s) 188, 419
BciT130I CCWGG 1 cut(s) 842
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 908
BfuAI ACCTGC 1 cut(s) 862
BisI GCNGC 4 cut(s) 244, 294, 326, 635
BlsI GCNGC 4 cut(s) 245, 295, 327, 636
BmcAI AGTACT 1 cut(s) 885
Bme1390I CCNGG 2 cut(s) 156, 842
Bme18I GGWCC 2 cut(s) 756, 821
BmeT110I CYCGRG 2 cut(s) 70, 303
BmgT120I GGNCC 2 cut(s) 756, 821
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 2 cut(s) 156, 842
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 3 cut(s) 424, 442, 557
BmuI ACTGGG 1 cut(s) 478
Bpu10I CCTNAGC 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 685
BsaJI CCNNGG 3 cut(s) 71, 252, 841
BsaWI WCCGGW 2 cut(s) 355, 823
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 858, 888
Bsc4I CCNNNNNNNGG 2 cut(s) 633, 847
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 484, 554
Bse3DI GCAATG 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 823
BseBI CCWGG 1 cut(s) 842
BseDI CCNNGG 3 cut(s) 71, 252, 841
BseGI GGATG 3 cut(s) 87, 343, 534
BseLI CCNNNNNNNGG 2 cut(s) 633, 847
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 3 cut(s) 305, 540, 692
BseNI ACTGG 2 cut(s) 484, 554
BseRI GAGGAG 2 cut(s) 141, 169
BseXI GCAGC 3 cut(s) 230, 280, 312
Bsh1236I CGCG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 3 cut(s) 321, 832, 840
BshTI ACCGGT 1 cut(s) 355
BsiEI CGRYCG 1 cut(s) 360
BsiHKCI CYCGRG 2 cut(s) 70, 303
BsiSI CCGG 3 cut(s) 156, 356, 824
BslFI GGGAC 2 cut(s) 466, 654
BslI CCNNNNNNNGG 2 cut(s) 633, 847
BsmAI GTCTC 1 cut(s) 211
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 2 cut(s) 466, 654
BsnI GGCC 3 cut(s) 321, 832, 840
BsoBI CYCGRG 2 cut(s) 70, 303
Bsp13I TCCGGA 1 cut(s) 823
Bsp1407I TGTACA 1 cut(s) 646
Bsp143I GATC 3 cut(s) 75, 84, 775
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 3 cut(s) 201, 335, 634
BspANI GGCC 3 cut(s) 321, 832, 840
BspCNI CTCAG 3 cut(s) 306, 539, 693
BspEI TCCGGA 1 cut(s) 823
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 1 cut(s) 777
BspMI ACCTGC 1 cut(s) 862
BspPI GGATC 3 cut(s) 83, 770, 783
BsrDI GCAATG 1 cut(s) 723
BsrFI RCCGGY 1 cut(s) 355
BsrGI TGTACA 1 cut(s) 646
BsrI ACTGG 2 cut(s) 484, 554
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 3 cut(s) 71, 252, 841
BssMI GATC 3 cut(s) 75, 84, 775
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 842
Bst4CI ACNGT 2 cut(s) 388, 654
BstAUI TGTACA 1 cut(s) 646
BstBAI YACGTR 1 cut(s) 685
BstC8I GCNNGC 4 cut(s) 286, 323, 834, 838
BstDEI CTNAG 6 cut(s) 314, 416, 469, 526, 701, 729
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 3 cut(s) 87, 343, 534
BstFNI CGCG 1 cut(s) 337
BstKTI GATC 3 cut(s) 78, 87, 778
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 3 cut(s) 75, 84, 775
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 4 cut(s) 270, 290, 299, 334
BstNI CCWGG 1 cut(s) 842
BstSCI CCNGG 2 cut(s) 154, 840
BstSNI TACGTA 1 cut(s) 685
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 3 cut(s) 230, 280, 312
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
BsuRI GGCC 3 cut(s) 321, 832, 840
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 3 cut(s) 87, 343, 534
BtsIMutI CAGTG 2 cut(s) 393, 547
BveI ACCTGC 1 cut(s) 862
Cac8I GCNNGC 4 cut(s) 286, 323, 834, 838
CciI TCATGA 1 cut(s) 852
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 2 cut(s) 756, 821
CseI GACGC 1 cut(s) 326
Csp6I GTAC 5 cut(s) 117, 647, 715, 884, 914
CspAI ACCGGT 1 cut(s) 355
CviAII CATG 5 cut(s) 44, 79, 253, 478, 853
CviQI GTAC 5 cut(s) 117, 647, 715, 884, 914
DdeI CTNAG 6 cut(s) 314, 416, 469, 526, 701, 729
DpnI GATC 3 cut(s) 77, 86, 777
DpnII GATC 3 cut(s) 75, 84, 775
EciI GGCGGA 1 cut(s) 190
Eco105I TACGTA 1 cut(s) 685
Eco130I CCWWGG 1 cut(s) 252
Eco147I AGGCCT 2 cut(s) 321, 832
Eco47I GGWCC 2 cut(s) 756, 821
Eco88I CYCGRG 2 cut(s) 70, 303
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 5 cut(s) 47, 82, 256, 481, 856
FaqI GGGAC 2 cut(s) 466, 654
FatI CATG 5 cut(s) 43, 78, 252, 477, 852
Fnu4HI GCNGC 4 cut(s) 244, 294, 326, 635
FokI GGATG 3 cut(s) 74, 330, 521
Fsp4HI GCNGC 4 cut(s) 244, 294, 326, 635
FspBI CTAG 1 cut(s) 908
GluI GCNGC 4 cut(s) 244, 294, 326, 635
HaeIII GGCC 3 cut(s) 321, 832, 840
HapII CCGG 3 cut(s) 156, 356, 824
HgaI GACGC 1 cut(s) 326
Hin1II CATG 5 cut(s) 47, 82, 256, 481, 856
HindIII AAGCTT 2 cut(s) 366, 443
HpaII CCGG 3 cut(s) 156, 356, 824
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 1 cut(s) 759
Hpy188I TCNGA 4 cut(s) 270, 315, 785, 932
Hpy188III TCNNGA 4 cut(s) 361, 700, 824, 853
Hpy8I GTNNAC 1 cut(s) 759
HpyAV CCTTC 4 cut(s) 156, 339, 415, 637
HpyCH4III ACNGT 2 cut(s) 388, 654
HpyCH4IV ACGT 1 cut(s) 684
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 290, 299, 334
HpyF3I CTNAG 6 cut(s) 314, 416, 469, 526, 701, 729
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 5 cut(s) 47, 82, 256, 481, 856
Kpn2I TCCGGA 1 cut(s) 823
Kzo9I GATC 3 cut(s) 75, 84, 775
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 3 cut(s) 230, 280, 312
LweI GCATC 3 cut(s) 424, 442, 557
MaeI CTAG 1 cut(s) 908
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 1 cut(s) 661
MalI GATC 3 cut(s) 77, 86, 777
MboI GATC 3 cut(s) 75, 84, 775
MboII GAAGA 2 cut(s) 735, 895
MflI RGATCY 1 cut(s) 775
MluCI AATT 6 cut(s) 48, 61, 92, 513, 618, 923
MroI TCCGGA 1 cut(s) 823
MseI TTAA 2 cut(s) 399, 522
MspI CCGG 3 cut(s) 156, 356, 824
MspR9I CCNGG 2 cut(s) 156, 842
MvaI CCWGG 1 cut(s) 842
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 4 cut(s) 270, 290, 299, 334
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 252
NdeII GATC 3 cut(s) 75, 84, 775
NlaIII CATG 5 cut(s) 47, 82, 256, 481, 856
NlaIV GGNNCC 1 cut(s) 777
PaeR7I CTCGAG 1 cut(s) 303
PagI TCATGA 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 862
PceI AGGCCT 2 cut(s) 321, 832
PinAI ACCGGT 1 cut(s) 355
PkrI GCNGC 4 cut(s) 245, 295, 327, 636
Ppu21I YACGTR 1 cut(s) 685
PsiI TTATAA 1 cut(s) 512
Psp6I CCWGG 1 cut(s) 840
PspGI CCWGG 1 cut(s) 840
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 2 cut(s) 756, 821
PspXI VCTCGAGB 1 cut(s) 303
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 5 cut(s) 118, 648, 716, 885, 915
RsaNI GTAC 5 cut(s) 117, 647, 715, 884, 914
SaqAI TTAA 2 cut(s) 399, 522
SatI GCNGC 4 cut(s) 244, 294, 326, 635
Sau3AI GATC 3 cut(s) 75, 84, 775
Sau96I GGNCC 2 cut(s) 756, 821
ScaI AGTACT 1 cut(s) 885
ScrFI CCNGG 2 cut(s) 156, 842
SfaNI GCATC 3 cut(s) 424, 442, 557
Sfr274I CTCGAG 1 cut(s) 303
SinI GGWCC 2 cut(s) 756, 821
SlaI CTCGAG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
SnaBI TACGTA 1 cut(s) 685
Sse9I AATT 6 cut(s) 48, 61, 92, 513, 618, 923
SseBI AGGCCT 2 cut(s) 321, 832
SsiI CCGC 3 cut(s) 201, 335, 634
SspMI CTAG 1 cut(s) 908
StuI AGGCCT 2 cut(s) 321, 832
StyD4I CCNGG 2 cut(s) 154, 840
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 2 cut(s) 388, 654
TaiI ACGT 1 cut(s) 687
TaqI TCGA 3 cut(s) 304, 360, 432
TasI AATT 6 cut(s) 48, 61, 92, 513, 618, 923
TatI WGTACW 2 cut(s) 646, 883
TauI GCSGC 1 cut(s) 637
Tru1I TTAA 2 cut(s) 399, 522
Tru9I TTAA 2 cut(s) 399, 522
TscAI CASTG 2 cut(s) 393, 554
TseI GCWGC 3 cut(s) 243, 293, 325
TspDTI ATGAA 5 cut(s) 24, 94, 252, 398, 466
TspGWI ACGGA 3 cut(s) 172, 179, 186
TspRI CASTG 2 cut(s) 393, 554
VpaK11BI GGWCC 2 cut(s) 756, 821
XapI RAATTY 4 cut(s) 48, 61, 618, 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 546
XhoI CTCGAG 1 cut(s) 303
XspI CTAG 1 cut(s) 908
ZrmI AGTACT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.