pycom08g20950

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Forward (+)
20535136 .. 20535782
647 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 366 bp
ATGCATCATCCTACGACTCCAGCATGCATGTCTACATATTTATGTGATGGCGCCACTCCTACAACTTCAAGAAATATATCCTTCCTTGGTGAAAAGTGCCCTACCAATCCAACAAATGCAACCCTATTTGATTTTGGAATGTTTAGTAGTGCCCTCGAGTCCGGTACCACGCAGTCAATGAACATTCCACCAAGGATCTTTTACTGTTTTTGGTGGGCTCTGAGAAATTTAAGTGAAATTTTGCAGTTCTTTTGGTCAGAATCTTCAAACAAGTACTTATGTGTGGGAAAACGGACTTGCAATTCTTATTTGTGTCATGGGTTTGCTACTGTTTCTACACCTTATTGGGAATGTGCAGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.68

Weight (kDa)

6.79

Isoelectric Point (pI)

65.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 347
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 2 cut(s) 50, 164
AccI GTMKAC 1 cut(s) 32
AclWI GGATC 1 cut(s) 203
AcsI RAATTY 2 cut(s) 226, 237
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 166, 275
AgsI TTSAA 2 cut(s) 69, 267
AlwI GGATC 1 cut(s) 203
Ama87I CYCGRG 1 cut(s) 155
ApoI RAATTY 2 cut(s) 226, 237
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 155
BaeGI GKGCMC 2 cut(s) 101, 154
BanI GGYRCC 2 cut(s) 50, 164
BanII GRGCYC 1 cut(s) 220
BccI CCATC 1 cut(s) 41
BfoI RGCGCY 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 347
BmcAI AGTACT 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 155
BmiI GGNNCC 2 cut(s) 52, 166
BmsI GCATC 1 cut(s) 13
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 85, 191
BsaWI WCCGGW 1 cut(s) 161
BseDI CCNNGG 2 cut(s) 85, 191
BseGI GGATG 1 cut(s) 7
BseMII CTCAG 1 cut(s) 212
BseSI GKGCMC 2 cut(s) 101, 154
BshNI GGYRCC 2 cut(s) 50, 164
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 162
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 3 cut(s) 101, 154, 220
Bsp143I GATC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 52, 166
BspMI ACCTGC 1 cut(s) 347
BspPI GGATC 1 cut(s) 203
BspT107I GGYRCC 2 cut(s) 50, 164
BssECI CCNNGG 2 cut(s) 85, 191
BssMI GATC 1 cut(s) 195
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 85, 191
Bst4CI ACNGT 2 cut(s) 206, 331
BstACI GRCGYC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 1 cut(s) 221
BstF5I GGATG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 1 cut(s) 198
BstMBI GATC 1 cut(s) 195
BstNSI RCATGY 2 cut(s) 27, 31
BstSLI GKGCMC 2 cut(s) 101, 154
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BtsCI GGATG 1 cut(s) 7
BveI ACCTGC 1 cut(s) 347
Cac8I GCNNGC 1 cut(s) 25
CfoI GCGC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 165, 274
CviAII CATG 3 cut(s) 24, 28, 317
CviJI RGCY 1 cut(s) 218
CviKI_1 RGCY 1 cut(s) 218
CviQI GTAC 2 cut(s) 165, 274
DdeI CTNAG 1 cut(s) 221
DinI GGCGCC 1 cut(s) 52
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 85, 191
Eco24I GRGCYC 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 155
EcoT14I CCWWGG 2 cut(s) 85, 191
EcoT22I ATGCAT 2 cut(s) 6, 29
EcoT38I GRGCYC 1 cut(s) 220
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 85, 191
FaeI CATG 3 cut(s) 27, 31, 320
FaiI YATR 7 cut(s) 25, 29, 37, 43, 77, 280, 318
FatI CATG 3 cut(s) 23, 27, 316
FblI GTMKAC 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 220
GlaI GCGC 1 cut(s) 52
HaeII RGCGCY 1 cut(s) 54
HapII CCGG 1 cut(s) 162
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 3 cut(s) 27, 31, 320
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 3 cut(s) 16, 158, 260
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 222, 259
Hpy188III TCNNGA 1 cut(s) 69
Hpy8I GTNNAC 1 cut(s) 33
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 2 cut(s) 206, 331
HpyCH4V TGCA 6 cut(s) 4, 27, 119, 244, 300, 356
HpyF3I CTNAG 1 cut(s) 221
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 3 cut(s) 27, 31, 320
HspAI GCGC 1 cut(s) 51
KasI GGCGCC 1 cut(s) 50
KpnI GGTACC 1 cut(s) 168
Kzo9I GATC 1 cut(s) 195
LpnPI CCDG 3 cut(s) 33, 175, 342
LweI GCATC 1 cut(s) 13
MalI GATC 1 cut(s) 197
MboI GATC 1 cut(s) 195
MboII GAAGA 1 cut(s) 255
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 3 cut(s) 101, 154, 220
MluCI AATT 3 cut(s) 226, 237, 301
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 2 cut(s) 10, 167
MmeI TCCRAC 1 cut(s) 134
MnlI CCTC 1 cut(s) 164
Mph1103I ATGCAT 2 cut(s) 6, 29
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 40
MspI CCGG 1 cut(s) 162
NarI GGCGCC 1 cut(s) 51
NdeII GATC 1 cut(s) 195
NlaIII CATG 3 cut(s) 27, 31, 320
NlaIV GGNNCC 2 cut(s) 52, 166
NsiI ATGCAT 2 cut(s) 6, 29
NspI RCATGY 2 cut(s) 27, 31
PaeI GCATGC 1 cut(s) 27
PaeR7I CTCGAG 1 cut(s) 155
PfeI GAWTC 1 cut(s) 260
PleI GAGTC 2 cut(s) 10, 166
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 2 cut(s) 10, 166
PspN4I GGNNCC 2 cut(s) 52, 166
PspXI VCTCGAGB 1 cut(s) 155
PsuI RGATCY 1 cut(s) 195
RsaI GTAC 2 cut(s) 166, 275
RsaNI GTAC 2 cut(s) 165, 274
RseI CAYNNNNRTG 1 cut(s) 40
SaqAI TTAA 1 cut(s) 230
Sau3AI GATC 1 cut(s) 195
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 10, 167
SduI GDGCHC 3 cut(s) 101, 154, 220
SetI ASST 2 cut(s) 343, 361
SfaNI GCATC 1 cut(s) 13
SfoI GGCGCC 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 155
SlaI CTCGAG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SphI GCATGC 1 cut(s) 27
Sse9I AATT 3 cut(s) 226, 237, 301
SspDI GGCGCC 1 cut(s) 50
StyI CCWWGG 2 cut(s) 85, 191
TaaI ACNGT 2 cut(s) 206, 331
TaqI TCGA 1 cut(s) 156
TasI AATT 3 cut(s) 226, 237, 301
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TspDTI ATGAA 1 cut(s) 194
TspGWI ACGGA 1 cut(s) 307
XapI RAATTY 2 cut(s) 226, 237
XceI RCATGY 2 cut(s) 27, 31
XhoI CTCGAG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 32
ZrmI AGTACT 1 cut(s) 275
Zsp2I ATGCAT 2 cut(s) 6, 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.