pycom09g00270

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Forward (+)
241914 .. 242765
852 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 852 bp
ATGTGCATGGATCTTTCTTTGTGTATGGGCCGGATCAATTATCTTTGGGCCAAGACAAGATTACAATTCAACAAACCCTACTGTGCTGTCGCCTCATCAGCCACCTCATCTTCCCTAAACCTCAAGGCAAGAAAGCAAGGGACCCACGTATTCTCTGCTGGTTTCTTCACCTTCAAGCCTCTCAAGCAACCCGGCTTATGCAACCAGAAACCCAGCATTCAGAATTCCAAGAAATTAAATTCCCAGAAGTTCTCATTCCCTCCTACTGATGAGCTGGCATTCCCATCCCCCTCTCAGGCGGACCCCACAATGCCTGATCTCCCCCCCGTCCCTAGGATCCCTTATCTTCCACCACTGCCACAGCTTCCTCCTCTCCCTCCCACCCCAACTGGTCTCCCAGGAGGTCTCCCAGGCCTCCCTGGACTCCCAGGCCTCCCAGGTCTCCCAGGCCTCCCAGGCCTCCCAGGACTTCCTGGAATTCCAGGTTCTCCCCTTGCTGCTGGAAAAACTTCATCTGGGTCGTCCAACTCCAAAACTTCAAAGAATGGGCAACTAGGTGATAATAAAGAAGTAGCCCATCCAGATACTTTCTTTCCACCAATTATCCCTAATCCATTTCAGCCCCCACCAGCTCCATTAATTCCTAATTTGCCTCCTCTTCCAGGTTCTCCACCAGCAGGTCCAGCACCATTGATTCCCAACCTGCCACCTATACCTGGTTTCACTCCATCGCCGTCGCCGCCACCACCAACCCTCCCATTCCTCCCTCCATTCCCATTCCCCCTCCCACCTACGCCTCGGATCCCTGGAATCCCCCCAGCTTCTTCTTCAAAACAAGGCACTTCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

284

Amino Acids

29.38

Weight (kDa)

9.85

Isoelectric Point (pI)

70.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 668, 711
AciI CCGC 2 cut(s) 299, 740
AclWI GGATC 6 cut(s) 18, 41, 331, 344, 796, 809
AcsI RAATTY 3 cut(s) 223, 238, 477
AfiI CCNNNNNNNGG 5 cut(s) 295, 333, 662, 677, 716
AgsI TTSAA 4 cut(s) 70, 175, 540, 831
AluBI AGCT 4 cut(s) 274, 364, 632, 821
AluI AGCT 4 cut(s) 274, 364, 632, 821
Alw26I GTCTC 3 cut(s) 398, 410, 446
AlwI GGATC 6 cut(s) 18, 41, 331, 344, 796, 809
AoxI GGCC 6 cut(s) 28, 48, 412, 430, 448, 457
ApeKI GCWGC 1 cut(s) 497
ApoI RAATTY 3 cut(s) 223, 238, 477
AseI ATTAAT 1 cut(s) 638
Asp700I GAANNNNTTC 1 cut(s) 508
AspA2I CCTAGG 1 cut(s) 332
AspS9I GGNCC 5 cut(s) 28, 48, 141, 301, 680
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 3 cut(s) 160, 569, 837
AvaII GGWCC 3 cut(s) 141, 301, 680
AvrII CCTAGG 1 cut(s) 332
BamHI GGATCC 2 cut(s) 336, 801
BbvI GCAGC 1 cut(s) 484
BccI CCATC 3 cut(s) 292, 585, 736
BceAI ACGGC 1 cut(s) 718
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 3 cut(s) 398, 410, 446
BfaI CTAG 2 cut(s) 333, 554
BfuAI ACCTGC 2 cut(s) 668, 711
BglI GCCNNNNNGGC 1 cut(s) 456
BisI GCNGC 2 cut(s) 498, 740
BlnI CCTAGG 1 cut(s) 332
BlsI GCNGC 2 cut(s) 499, 741
Bme18I GGWCC 3 cut(s) 141, 301, 680
BmgT120I GGNCC 5 cut(s) 28, 48, 141, 301, 680
BmiI GGNNCC 5 cut(s) 142, 143, 303, 338, 803
BpuEI CTTGAG 2 cut(s) 107, 167
BpuMI CCSGG 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 148
BsaI GGTCTC 3 cut(s) 398, 410, 446
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 5 cut(s) 295, 333, 662, 677, 716
Bse1I ACTGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 284, 577
BseLI CCNNNNNNNGG 5 cut(s) 295, 333, 662, 677, 716
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 2 cut(s) 360, 645
BseXI GCAGC 1 cut(s) 484
BseYI CCCAGC 2 cut(s) 212, 817
BshFI GGCC 6 cut(s) 30, 50, 414, 432, 450, 459
BsiSI CCGG 2 cut(s) 31, 192
BslFI GGGAC 2 cut(s) 154, 314
BslI CCNNNNNNNGG 5 cut(s) 295, 333, 662, 677, 716
BsmAI GTCTC 3 cut(s) 398, 410, 446
BsmFI GGGAC 2 cut(s) 154, 314
BsmI GAATGC 2 cut(s) 216, 278
BsnI GGCC 6 cut(s) 30, 50, 414, 432, 450, 459
Bso31I GGTCTC 3 cut(s) 398, 410, 446
Bsp143I GATC 5 cut(s) 10, 33, 316, 336, 801
BspACI CCGC 2 cut(s) 299, 740
BspANI GGCC 6 cut(s) 30, 50, 414, 432, 450, 459
BspCNI CTCAG 1 cut(s) 307
BspLI GGNNCC 5 cut(s) 142, 143, 303, 338, 803
BspMI ACCTGC 2 cut(s) 668, 711
BspPI GGATC 6 cut(s) 18, 41, 331, 344, 796, 809
BspTNI GGTCTC 3 cut(s) 398, 410, 446
BsrI ACTGG 1 cut(s) 394
BssMI GATC 5 cut(s) 10, 33, 316, 336, 801
BssT1I CCWWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 1 cut(s) 663
BstBAI YACGTR 1 cut(s) 148
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 1 cut(s) 294
BstENI CCTNNNNNAGG 1 cut(s) 660
BstF5I GGATG 2 cut(s) 284, 577
BstKTI GATC 5 cut(s) 13, 36, 319, 339, 804
BstMAI GTCTC 3 cut(s) 398, 410, 446
BstMBI GATC 5 cut(s) 10, 33, 316, 336, 801
BstMWI GCNNNNNNNGC 5 cut(s) 98, 184, 456, 683, 739
BstV1I GCAGC 1 cut(s) 484
BstX2I RGATCY 3 cut(s) 10, 336, 801
BstYI RGATCY 3 cut(s) 10, 336, 801
BsuRI GGCC 6 cut(s) 30, 50, 414, 432, 450, 459
BtgZI GCGATG 1 cut(s) 714
BtsCI GGATG 2 cut(s) 284, 577
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 353
BveI ACCTGC 2 cut(s) 668, 711
Cac8I GCNNGC 1 cut(s) 276
Cfr13I GGNCC 5 cut(s) 28, 48, 141, 301, 680
CsiI ACCWGGT 1 cut(s) 715
CviAII CATG 1 cut(s) 7
DdeI CTNAG 1 cut(s) 294
DpnI GATC 5 cut(s) 12, 35, 318, 338, 803
DpnII GATC 5 cut(s) 10, 33, 316, 336, 801
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
EciI GGCGGA 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 332
Eco147I AGGCCT 4 cut(s) 414, 432, 450, 459
Eco31I GGTCTC 3 cut(s) 398, 410, 446
Eco47I GGWCC 3 cut(s) 141, 301, 680
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoO109I RGGNCCY 1 cut(s) 141
EcoRI GAATTC 2 cut(s) 223, 477
EcoT14I CCWWGG 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 332
FaeI CATG 1 cut(s) 10
FaiI YATR 4 cut(s) 8, 26, 199, 713
FaqI GGGAC 2 cut(s) 154, 314
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 498, 740
FokI GGATG 2 cut(s) 271, 564
Fsp4HI GCNGC 2 cut(s) 498, 740
FspBI CTAG 2 cut(s) 333, 554
GluI GCNGC 2 cut(s) 498, 740
GsaI CCCAGC 2 cut(s) 216, 821
HaeIII GGCC 6 cut(s) 30, 50, 414, 432, 450, 459
HapII CCGG 2 cut(s) 31, 192
Hin1II CATG 1 cut(s) 10
HinfI GANTC 3 cut(s) 423, 694, 810
HpaII CCGG 2 cut(s) 31, 192
HphI GGTGA 3 cut(s) 160, 569, 837
Hpy188I TCNGA 2 cut(s) 222, 801
Hpy188III TCNNGA 1 cut(s) 581
Hpy99I CGWCG 1 cut(s) 739
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 6, 201
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 184, 456, 683, 739
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 1 cut(s) 10
KflI GGGWCCC 1 cut(s) 141
Kzo9I GATC 5 cut(s) 10, 33, 316, 336, 801
LmnI GCTCC 1 cut(s) 637
Lsp1109I GCAGC 1 cut(s) 484
MabI ACCWGGT 1 cut(s) 715
MaeI CTAG 2 cut(s) 333, 554
MaeII ACGT 1 cut(s) 147
MalI GATC 5 cut(s) 12, 35, 318, 338, 803
MboI GATC 5 cut(s) 10, 33, 316, 336, 801
MboII GAAGA 6 cut(s) 102, 157, 338, 650, 816, 819
MflI RGATCY 3 cut(s) 10, 336, 801
MluCI AATT 9 cut(s) 37, 65, 223, 233, 238, 477, 600, 639, 646
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 1 cut(s) 549
MroXI GAANNNNTTC 1 cut(s) 508
MseI TTAA 2 cut(s) 236, 638
MspI CCGG 2 cut(s) 31, 192
Mva1269I GAATGC 2 cut(s) 216, 278
MwoI GCNNNNNNNGC 5 cut(s) 98, 184, 456, 683, 739
NciI CCSGG 1 cut(s) 192
NdeII GATC 5 cut(s) 10, 33, 316, 336, 801
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 5 cut(s) 142, 143, 303, 338, 803
PceI AGGCCT 4 cut(s) 414, 432, 450, 459
PctI GAATGC 2 cut(s) 216, 278
PdmI GAANNNNTTC 1 cut(s) 508
PfeI GAWTC 2 cut(s) 694, 810
PfoI TCCNGGA 1 cut(s) 472
PkrI GCNGC 2 cut(s) 499, 741
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
Ppu21I YACGTR 1 cut(s) 148
PpuMI RGGWCCY 1 cut(s) 141
PshBI ATTAAT 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 141
PspFI CCCAGC 2 cut(s) 212, 817
PspN4I GGNNCC 5 cut(s) 142, 143, 303, 338, 803
PspPI GGNCC 5 cut(s) 28, 48, 141, 301, 680
PspPPI RGGWCCY 1 cut(s) 141
PsuI RGATCY 3 cut(s) 10, 336, 801
SaqAI TTAA 2 cut(s) 236, 638
SatI GCNGC 2 cut(s) 498, 740
Sau3AI GATC 5 cut(s) 10, 33, 316, 336, 801
Sau96I GGNCC 5 cut(s) 28, 48, 141, 301, 680
SchI GAGTC 1 cut(s) 417
SexAI ACCWGGT 1 cut(s) 715
SfiI GGCCNNNNNGGCC 1 cut(s) 456
SinI GGWCC 3 cut(s) 141, 301, 680
SmlI CTYRAG 2 cut(s) 122, 182
SmoI CTYRAG 2 cut(s) 122, 182
Sse9I AATT 9 cut(s) 37, 65, 223, 233, 238, 477, 600, 639, 646
SseBI AGGCCT 4 cut(s) 414, 432, 450, 459
SsiI CCGC 2 cut(s) 299, 740
SspMI CTAG 2 cut(s) 333, 554
StuI AGGCCT 4 cut(s) 414, 432, 450, 459
StyI CCWWGG 1 cut(s) 332
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 1 cut(s) 150
TasI AATT 9 cut(s) 37, 65, 223, 233, 238, 477, 600, 639, 646
TauI GCSGC 1 cut(s) 742
TfiI GAWTC 2 cut(s) 694, 810
Tru1I TTAA 2 cut(s) 236, 638
Tru9I TTAA 2 cut(s) 236, 638
TscAI CASTG 1 cut(s) 360
TseI GCWGC 1 cut(s) 497
TspDTI ATGAA 1 cut(s) 501
TspRI CASTG 1 cut(s) 360
VpaK11BI GGWCC 3 cut(s) 141, 301, 680
VspI ATTAAT 1 cut(s) 638
XagI CCTNNNNNAGG 1 cut(s) 660
XapI RAATTY 3 cut(s) 223, 238, 477
XmaJI CCTAGG 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 508
XspI CTAG 2 cut(s) 333, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.