Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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No orthologs found for this gene in the current database.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 67 |
| AcsI | RAATTY | 1 cut(s) | 54 |
| AcuI | CTGAAG | 1 cut(s) | 182 |
| AleI | CACNNNNGTG | 1 cut(s) | 24 |
| AluBI | AGCT | 2 cut(s) | 34, 127 |
| AluI | AGCT | 2 cut(s) | 34, 127 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 135 |
| AoxI | GGCC | 1 cut(s) | 170 |
| ApoI | RAATTY | 1 cut(s) | 54 |
| BfaI | CTAG | 1 cut(s) | 48 |
| BfmI | CTRYAG | 1 cut(s) | 61 |
| BmsI | GCATC | 1 cut(s) | 98 |
| BseMII | CTCAG | 1 cut(s) | 142 |
| Bsh1285I | CGRYCG | 1 cut(s) | 121 |
| BshFI | GGCC | 1 cut(s) | 172 |
| BsiEI | CGRYCG | 1 cut(s) | 121 |
| BsnI | GGCC | 1 cut(s) | 172 |
| Bsp143I | GATC | 1 cut(s) | 118 |
| BspANI | GGCC | 1 cut(s) | 172 |
| BspCNI | CTCAG | 1 cut(s) | 141 |
| BssMI | GATC | 1 cut(s) | 118 |
| Bst4CI | ACNGT | 1 cut(s) | 26 |
| Bst6I | CTCTTC | 1 cut(s) | 158 |
| BstC8I | GCNNGC | 3 cut(s) | 125, 143, 174 |
| BstDEI | CTNAG | 1 cut(s) | 128 |
| BstKTI | GATC | 1 cut(s) | 121 |
| BstMBI | GATC | 1 cut(s) | 118 |
| BstMCI | CGRYCG | 1 cut(s) | 121 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 95 |
| BstSFI | CTRYAG | 1 cut(s) | 61 |
| BsuRI | GGCC | 1 cut(s) | 172 |
| Cac8I | GCNNGC | 3 cut(s) | 125, 143, 174 |
| CaiI | CAGNNNCTG | 1 cut(s) | 135 |
| CviJI | RGCY | 5 cut(s) | 34, 98, 127, 158, 172 |
| CviKI_1 | RGCY | 5 cut(s) | 34, 98, 127, 158, 172 |
| DdeI | CTNAG | 1 cut(s) | 128 |
| DpnI | GATC | 1 cut(s) | 120 |
| DpnII | GATC | 1 cut(s) | 118 |
| Eam1104I | CTCTTC | 1 cut(s) | 158 |
| EarI | CTCTTC | 1 cut(s) | 158 |
| Eco57I | CTGAAG | 1 cut(s) | 182 |
| FaiI | YATR | 2 cut(s) | 178, 180 |
| FauNDI | CATATG | 1 cut(s) | 178 |
| FblI | GTMKAC | 1 cut(s) | 67 |
| FspBI | CTAG | 1 cut(s) | 48 |
| HaeIII | GGCC | 1 cut(s) | 172 |
| HincII | GTYRAC | 1 cut(s) | 68 |
| HindII | GTYRAC | 1 cut(s) | 68 |
| HindIII | AAGCTT | 1 cut(s) | 32 |
| HinfI | GANTC | 1 cut(s) | 132 |
| Hpy166II | GTNNAC | 1 cut(s) | 68 |
| Hpy188I | TCNGA | 1 cut(s) | 131 |
| Hpy8I | GTNNAC | 1 cut(s) | 68 |
| HpyAV | CCTTC | 1 cut(s) | 162 |
| HpyCH4III | ACNGT | 1 cut(s) | 26 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 95 |
| HpyF3I | CTNAG | 1 cut(s) | 128 |
| Kzo9I | GATC | 1 cut(s) | 118 |
| LpnPI | CCDG | 3 cut(s) | 121, 137, 173 |
| LweI | GCATC | 1 cut(s) | 98 |
| MaeI | CTAG | 1 cut(s) | 48 |
| MaeIII | GTNAC | 1 cut(s) | 40 |
| MalI | GATC | 1 cut(s) | 120 |
| MboI | GATC | 1 cut(s) | 118 |
| MboII | GAAGA | 1 cut(s) | 175 |
| MluCI | AATT | 1 cut(s) | 54 |
| MnlI | CCTC | 1 cut(s) | 24 |
| MseI | TTAA | 1 cut(s) | 5 |
| MslI | CAYNNNNRTG | 1 cut(s) | 24 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 95 |
| NdeI | CATATG | 1 cut(s) | 178 |
| NdeII | GATC | 1 cut(s) | 118 |
| OliI | CACNNNNGTG | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 132 |
| Ple19I | CGATCG | 1 cut(s) | 121 |
| PstNI | CAGNNNCTG | 1 cut(s) | 135 |
| PvuI | CGATCG | 1 cut(s) | 121 |
| RseI | CAYNNNNRTG | 1 cut(s) | 24 |
| SalI | GTCGAC | 1 cut(s) | 66 |
| SaqAI | TTAA | 1 cut(s) | 5 |
| Sau3AI | GATC | 1 cut(s) | 118 |
| SetI | ASST | 2 cut(s) | 36, 129 |
| SfaNI | GCATC | 1 cut(s) | 98 |
| SfcI | CTRYAG | 1 cut(s) | 61 |
| SgeI | CNNG | 9 cut(s) | 27, 60, 98, 136, 148, 154, 158, 172, 185 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 24 |
| Sse9I | AATT | 1 cut(s) | 54 |
| SspMI | CTAG | 1 cut(s) | 48 |
| TaaI | ACNGT | 1 cut(s) | 26 |
| TaqI | TCGA | 1 cut(s) | 67 |
| TasI | AATT | 1 cut(s) | 54 |
| TfiI | GAWTC | 1 cut(s) | 132 |
| Tru1I | TTAA | 1 cut(s) | 5 |
| Tru9I | TTAA | 1 cut(s) | 5 |
| XapI | RAATTY | 1 cut(s) | 54 |
| XmiI | GTMKAC | 1 cut(s) | 67 |
| XspI | CTAG | 1 cut(s) | 48 |