pycom09g03920

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Forward (+)
2915787 .. 2916333
547 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 345 bp
ATGCGCCACTCCATTGTTATCAATCTTGGTGACCAACTTGAGGTGATCACCAACGGAAAGTACAAGAGTGTGGAACACAGGGTGATTGCCCAAACAGATGGCACCAGAATGTCAATAGCTTCATTCTACAACCCAGGCAGTGATGCGGTGATCTACCCAGCACCAACCCTAGTGGAGAAAGAAGCAGAGGAGAAGAATCAAGTGTACCCGAAGTTCGTGTTCGAAGACTACATGAAGCTCTATGCTGGGGTCAAGTTCGAGCCCAAGGAACCAAGATTTGAAGCCATGAAAGCAGTGGAAATTAAGGCCAGTTTTGGTTTGGGTCCAGTTATAAGTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009506 GO:0009593 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009735 GO:0009815 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010817 GO:0012505 GO:0016020 GO:0016491 GO:0016638 GO:0016641 GO:0030054 GO:0030312 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043449 GO:0043450 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048518 GO:0048580 GO:0048582 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0055114 GO:0065007 GO:0065008 GO:0070542 GO:0070887 GO:0071229 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071396 GO:0071398 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0097366 GO:1900140 GO:1900673 GO:1900674 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

115

Amino Acids

12.77

Weight (kDa)

6.11

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 2 - 45 1.3e-12 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 101
AciI CCGC 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 82
AfaI GTAC 3 cut(s) 62, 206, 337
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 1 cut(s) 281
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 2 cut(s) 119, 238
AluI AGCT 2 cut(s) 119, 238
AoxI GGCC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 5 cut(s) 40, 41, 55, 94, 160
AsuII TTCGAA 1 cut(s) 222
AvaII GGWCC 1 cut(s) 323
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 231
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 45
BfaI CTAG 1 cut(s) 170
BmcAI AGTACT 1 cut(s) 337
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 3 cut(s) 103, 270, 324
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 231
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 133, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 309, 326
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 133, 264
BseLI CCNNNNNNNGG 1 cut(s) 40
BseNI ACTGG 2 cut(s) 309, 326
BseRI GAGGAG 1 cut(s) 203
BseYI CCCAGC 2 cut(s) 157, 245
BshFI GGCC 1 cut(s) 308
BshNI GGYRCC 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 40
BsnI GGCC 1 cut(s) 308
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 2 cut(s) 45, 150
BspACI CCGC 1 cut(s) 146
BspANI GGCC 1 cut(s) 308
BspLI GGNNCC 3 cut(s) 103, 270, 324
BspT104I TTCGAA 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 101
BsrI ACTGG 2 cut(s) 309, 326
BssECI CCNNGG 2 cut(s) 133, 264
BssMI GATC 2 cut(s) 45, 150
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 135
BstBI TTCGAA 1 cut(s) 222
BstEII GGTNACC 1 cut(s) 29
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 2 cut(s) 48, 153
BstMBI GATC 2 cut(s) 45, 150
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstNI CCWGG 1 cut(s) 135
BstPI GGTNACC 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 133
BstV2I GAAGAC 1 cut(s) 231
BstXI CCANNNNNNTGG 1 cut(s) 98
BsuRI GGCC 1 cut(s) 308
BtsI GCAGTG 2 cut(s) 145, 300
BtsIMutI CAGTG 2 cut(s) 145, 300
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 3 cut(s) 61, 205, 336
CspCI CAANNNNNGTGG 2 cut(s) 153, 188
CviAII CATG 2 cut(s) 232, 286
CviJI RGCY 5 cut(s) 119, 238, 262, 284, 308
CviKI_1 RGCY 5 cut(s) 119, 238, 262, 284, 308
CviQI GTAC 3 cut(s) 61, 205, 336
DpnI GATC 2 cut(s) 47, 152
DpnII GATC 2 cut(s) 45, 150
DraIII CACNNNGTG 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 323
Eco91I GGTNACC 1 cut(s) 29
EcoO65I GGTNACC 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 2 cut(s) 235, 289
FaiI YATR 4 cut(s) 233, 243, 287, 332
FalI AAGNNNNNCTT 1 cut(s) 325
FatI CATG 2 cut(s) 231, 285
FbaI TGATCA 1 cut(s) 45
FriOI GRGCYC 1 cut(s) 264
FspBI CTAG 1 cut(s) 170
GlaI GCGC 1 cut(s) 5
GsaI CCCAGC 2 cut(s) 161, 249
HaeIII GGCC 1 cut(s) 308
HhaI GCGC 1 cut(s) 6
Hin1II CATG 2 cut(s) 235, 289
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 1 cut(s) 196
HphI GGTGA 5 cut(s) 40, 41, 55, 94, 160
Hpy166II GTNNAC 1 cut(s) 205
Hpy8I GTNNAC 1 cut(s) 205
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
Hsp92II CATG 2 cut(s) 235, 289
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 2 cut(s) 45, 150
LpnPI CCDG 8 cut(s) 64, 118, 120, 147, 171, 231, 322, 339
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 47, 152
MboI GATC 2 cut(s) 45, 150
MboII GAAGA 2 cut(s) 205, 236
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 1 cut(s) 300
MnlI CCTC 2 cut(s) 34, 181
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 290
NdeII GATC 2 cut(s) 45, 150
NlaIII CATG 2 cut(s) 235, 289
NlaIV GGNNCC 3 cut(s) 103, 270, 324
NmuCI GTSAC 1 cut(s) 29
NspV TTCGAA 1 cut(s) 222
PfeI GAWTC 1 cut(s) 196
PsiI TTATAA 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 133
PspEI GGTNACC 1 cut(s) 29
PspFI CCCAGC 2 cut(s) 157, 245
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 3 cut(s) 103, 270, 324
PspPI GGNCC 1 cut(s) 323
PsrI GAACNNNNNNTAC 2 cut(s) 197, 229
RsaI GTAC 3 cut(s) 62, 206, 337
RsaNI GTAC 3 cut(s) 61, 205, 336
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 1 cut(s) 303
Sau3AI GATC 2 cut(s) 45, 150
Sau96I GGNCC 1 cut(s) 323
ScaI AGTACT 1 cut(s) 337
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 264
SetI ASST 3 cut(s) 45, 121, 240
SfaNI GCATC 1 cut(s) 133
SfuI TTCGAA 1 cut(s) 222
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 1 cut(s) 300
SsiI CCGC 1 cut(s) 146
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 264
TaqI TCGA 2 cut(s) 222, 258
TasI AATT 1 cut(s) 300
TatI WGTACW 2 cut(s) 60, 335
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 2 cut(s) 145, 300
TseFI GTSAC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 111, 248, 302
TspGWI ACGGA 1 cut(s) 69
TspRI CASTG 2 cut(s) 145, 300
VpaK11BI GGWCC 1 cut(s) 323
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 316
XspI CTAG 1 cut(s) 170
ZrmI AGTACT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.