pycom09g04520

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Forward (+)
3339136 .. 3340469
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGGCAAAAAAGTTGACAATCAAGGGTCTTTTCAAGCCTAAGGCGAGAACGCCGATAGAGCTTGTAAAGCACATGCGTGAGCTTCTAATGTTCGTTGATCAAAATAATGATGCGCGTGAGCAAAAGCGCAAAGAAAAGGATCGTAAAAACGCTACTCATGTCATTGCAAATTTACAAAGGCAAAAGGTTCAGTCGCGTTCGATCGCATTAGAATACTTGGAGAATAATATTGATATTATGGACATTCTGATAAAAGGCTATGAAGACAGTGGTGATATTGCTCTATCTTATGGTGCAATTTCAAGGGAATGCATTCGTCATCAGAATATTGCAAGGTATGTCCTCGAATCAGGTCACATGAAGAAGTTTTTTTATTACATTCAAACTCCAAATTTCGACATAGCATCAGATGCTGCGGCTACTTTCAAGGAGCTCATGACTAGGCACAAATCAACTGTTGCTGAATTCCTTTCTAAAAACTATGAGTGGGTAAGATTCATAATCTTCATGAAAACAAATTTCTGGTTGTTTGGAAGTTGGCTAGTATTTAGATGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

187

Amino Acids

22.01

Weight (kDa)

9.62

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 47 - 164 1.1e-40 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 115, 196
AciI CCGC 1 cut(s) 416
AclWI GGATC 1 cut(s) 147
AcsI RAATTY 4 cut(s) 169, 391, 464, 517
AgsI TTSAA 4 cut(s) 34, 303, 383, 427
AleI CACNNNNGTG 1 cut(s) 75
AluBI AGCT 3 cut(s) 61, 82, 433
AluI AGCT 3 cut(s) 61, 82, 433
Alw21I GWGCWC 1 cut(s) 435
AlwI GGATC 1 cut(s) 147
AlwNI CAGNNNCTG 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 4 cut(s) 169, 391, 464, 517
Asp700I GAANNNNTTC 1 cut(s) 312
AspLEI GCGC 2 cut(s) 115, 129
AsuHPI GGTGA 1 cut(s) 284
AxyI CCTNAGG 1 cut(s) 39
BanII GRGCYC 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 1 cut(s) 400
BclI TGATCA 1 cut(s) 97
BfaI CTAG 2 cut(s) 441, 542
BisI GCNGC 2 cut(s) 414, 417
BlsI GCNGC 2 cut(s) 415, 418
BmsI GCATC 4 cut(s) 100, 400, 413, 542
BpiI GAAGAC 1 cut(s) 270
Bse21I CCTNAGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 400
Bsh1236I CGCG 2 cut(s) 115, 196
Bsh1285I CGRYCG 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 435
BsmI GAATGC 2 cut(s) 312, 314
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 3 cut(s) 97, 139, 201
BspACI CCGC 1 cut(s) 416
BspFNI CGCG 2 cut(s) 115, 196
BspHI TCATGA 2 cut(s) 435, 507
BspPI GGATC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 162
BssMI GATC 3 cut(s) 97, 139, 201
Bst4CI ACNGT 2 cut(s) 269, 457
BstAPI GCANNNNNTGC 1 cut(s) 410
BstDEI CTNAG 1 cut(s) 39
BstFNI CGCG 2 cut(s) 115, 196
BstHHI GCGC 2 cut(s) 115, 129
BstKTI GATC 3 cut(s) 100, 142, 204
BstMBI GATC 3 cut(s) 97, 139, 201
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 58, 67, 410
BstNSI RCATGY 1 cut(s) 76
BstUI CGCG 2 cut(s) 115, 196
BstV1I GCAGC 1 cut(s) 400
BstV2I GAAGAC 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 274
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 2 cut(s) 435, 507
CfoI GCGC 2 cut(s) 115, 129
CviAII CATG 5 cut(s) 73, 158, 358, 436, 508
CviJI RGCY 7 cut(s) 37, 61, 82, 258, 419, 433, 541
CviKI_1 RGCY 7 cut(s) 37, 61, 82, 258, 419, 433, 541
DdeI CTNAG 1 cut(s) 39
DpnI GATC 3 cut(s) 99, 141, 203
DpnII GATC 3 cut(s) 97, 139, 201
Ecl136II GAGCTC 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 433
Eco81I CCTNAGG 1 cut(s) 39
EcoICRI GAGCTC 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 464
EcoT22I ATGCAT 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 435
FaeI CATG 5 cut(s) 76, 161, 361, 439, 511
FatI CATG 5 cut(s) 72, 157, 357, 435, 507
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 414, 417
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 2 cut(s) 414, 417
FspBI CTAG 2 cut(s) 441, 542
GlaI GCGC 2 cut(s) 114, 128
GluI GCNGC 2 cut(s) 414, 417
HhaI GCGC 2 cut(s) 115, 129
Hin1II CATG 5 cut(s) 76, 161, 361, 439, 511
Hin6I GCGC 2 cut(s) 113, 127
HinP1I GCGC 2 cut(s) 113, 127
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 2 cut(s) 347, 495
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 3 cut(s) 249, 324, 409
Hpy188III TCNNGA 2 cut(s) 436, 508
Hpy8I GTNNAC 1 cut(s) 15
HpyCH4III ACNGT 2 cut(s) 269, 457
HpyCH4V TGCA 4 cut(s) 167, 296, 312, 332
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 67, 410
HpyF3I CTNAG 1 cut(s) 39
Hsp92II CATG 5 cut(s) 76, 161, 361, 439, 511
HspAI GCGC 2 cut(s) 113, 127
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 3 cut(s) 97, 139, 201
LmnI GCTCC 1 cut(s) 430
LpnPI CCDG 2 cut(s) 336, 508
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 4 cut(s) 100, 400, 413, 542
MaeI CTAG 2 cut(s) 441, 542
MaeIII GTNAC 1 cut(s) 353
MalI GATC 3 cut(s) 99, 141, 203
MboI GATC 3 cut(s) 97, 139, 201
MboII GAAGA 3 cut(s) 275, 373, 496
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 5 cut(s) 169, 297, 391, 464, 517
MnlI CCTC 1 cut(s) 353
Mph1103I ATGCAT 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 312
MslI CAYNNNNRTG 1 cut(s) 75
Mva1269I GAATGC 2 cut(s) 312, 314
MvnI CGCG 2 cut(s) 115, 196
MwoI GCNNNNNNNGC 3 cut(s) 58, 67, 410
NdeII GATC 3 cut(s) 97, 139, 201
NlaIII CATG 5 cut(s) 76, 161, 361, 439, 511
NmuCI GTSAC 1 cut(s) 353
NsiI ATGCAT 1 cut(s) 314
NspI RCATGY 1 cut(s) 76
OliI CACNNNNGTG 1 cut(s) 75
PagI TCATGA 2 cut(s) 435, 507
PctI GAATGC 2 cut(s) 312, 314
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 347, 495
PkrI GCNGC 2 cut(s) 415, 418
Ple19I CGATCG 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 435
PstNI CAGNNNCTG 1 cut(s) 413
PvuI CGATCG 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 75
SacI GAGCTC 1 cut(s) 435
SatI GCNGC 2 cut(s) 414, 417
Sau3AI GATC 3 cut(s) 97, 139, 201
SduI GDGCHC 1 cut(s) 435
SetI ASST 6 cut(s) 63, 84, 189, 338, 355, 435
SfaNI GCATC 4 cut(s) 100, 400, 413, 542
SmiMI CAYNNNNRTG 1 cut(s) 75
Sse9I AATT 5 cut(s) 169, 297, 391, 464, 517
SsiI CCGC 1 cut(s) 416
SspI AATATT 2 cut(s) 229, 328
SspMI CTAG 2 cut(s) 441, 542
SstI GAGCTC 1 cut(s) 435
TaaI ACNGT 2 cut(s) 269, 457
TaqI TCGA 3 cut(s) 200, 345, 396
TasI AATT 5 cut(s) 169, 297, 391, 464, 517
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 347, 495
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 5 cut(s) 276, 374, 487, 496, 524
TspRI CASTG 1 cut(s) 274
XapI RAATTY 4 cut(s) 169, 391, 464, 517
XceI RCATGY 1 cut(s) 76
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 441, 542
Zsp2I ATGCAT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.