pycom09g06980

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Forward (+)
5160236 .. 5160989
754 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 309 bp
ATGATCAACGGCCATGAAACACTGGAGGCAGCACATAAAATTGATCCTCAAAATGTCAAGAAGGAGCAAAGGTTTCAAAAGCTTAAGATTGACCACAGAGATCAGGGTTGGAGGGAGAAGGTAGTCAGACTTCATTTGCTTTTGACCGTCAAGGAGTCTGCCATCAATGTCCCTCAAAACTTGGATGCTCGCAGGCGCATCACCTTCTTTGCAAACTCCTTATTCATGAACATGCCACGTGCTCCCAAAGTTCGTGACATGCTCTCATTCAGTGTTCTGACTCCTTATTACAAAGAAGATGTGCTCTAG

Protein Analysis

103

Amino Acids

12.07

Weight (kDa)

9.91

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CALS1 PF25968 8 - 50 2.4e-06 Callose synthase 1 domain
Glucan_synthase PF02364 57 - 102 7.6e-16 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 10
AcvI CACGTG 1 cut(s) 239
AflII CTTAAG 1 cut(s) 83
AgsI TTSAA 1 cut(s) 77
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw21I GWGCWC 2 cut(s) 244, 306
AlwI GGATC 1 cut(s) 38
AoxI GGCC 1 cut(s) 10
ApeKI GCWGC 1 cut(s) 29
AspLEI GCGC 1 cut(s) 198
AsuHPI GGTGA 1 cut(s) 193
BarI GAAGNNNNNNTAC 2 cut(s) 114, 146
BbrPI CACGTG 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 244, 306
BbvI GCAGC 1 cut(s) 41
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 25
BclI TGATCA 1 cut(s) 3
BfaI CTAG 1 cut(s) 307
BfrI CTTAAG 1 cut(s) 83
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmsI GCATC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 44
BsaAI YACGTR 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bse1I ACTGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 190
BseNI ACTGG 1 cut(s) 27
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 244, 306
BslFI GGGAC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 244, 306
Bsp143I GATC 3 cut(s) 3, 43, 100
BspANI GGCC 1 cut(s) 12
BspHI TCATGA 1 cut(s) 225
BspPI GGATC 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 83
BsrI ACTGG 1 cut(s) 27
BssMI GATC 3 cut(s) 3, 43, 100
Bst4CI ACNGT 1 cut(s) 148
BstAFI CTTAAG 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 190, 194
BstF5I GGATG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 3 cut(s) 6, 46, 103
BstMBI GATC 3 cut(s) 3, 43, 100
BstNSI RCATGY 2 cut(s) 235, 262
BstV1I GCAGC 1 cut(s) 41
BsuRI GGCC 1 cut(s) 12
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 20, 277
Cac8I GCNNGC 2 cut(s) 190, 194
CciI TCATGA 1 cut(s) 225
CfoI GCGC 1 cut(s) 198
CviAII CATG 4 cut(s) 14, 226, 232, 259
CviJI RGCY 2 cut(s) 12, 82
CviKI_1 RGCY 2 cut(s) 12, 82
DpnI GATC 3 cut(s) 5, 45, 102
DpnII GATC 3 cut(s) 3, 43, 100
EaeI YGGCCR 1 cut(s) 10
Eco72I CACGTG 1 cut(s) 239
FaeI CATG 4 cut(s) 17, 229, 235, 262
FaiI YATR 5 cut(s) 15, 36, 227, 233, 260
FaqI GGGAC 1 cut(s) 155
FatI CATG 4 cut(s) 13, 225, 231, 258
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 307
GlaI GCGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 1 cut(s) 12
HhaI GCGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 17, 229, 235, 262
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 2 cut(s) 155, 280
HphI GGTGA 1 cut(s) 193
Hpy188I TCNGA 2 cut(s) 128, 279
Hpy188III TCNNGA 3 cut(s) 58, 226, 254
HpyAV CCTTC 3 cut(s) 55, 112, 214
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 4 cut(s) 17, 229, 235, 262
HspAI GCGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 43, 100
LmnI GCTCC 2 cut(s) 64, 247
LpnPI CCDG 3 cut(s) 8, 89, 178
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 175, 207
MaeI CTAG 1 cut(s) 307
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 1 cut(s) 254
MalI GATC 3 cut(s) 5, 45, 102
MboI GATC 3 cut(s) 3, 43, 100
MboII GAAGA 1 cut(s) 308
MhlI GDGCHC 2 cut(s) 244, 306
MluCI AATT 1 cut(s) 39
MlyI GAGTC 2 cut(s) 164, 274
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 4 cut(s) 19, 57, 105, 183
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 230
MspCI CTTAAG 1 cut(s) 83
NdeII GATC 3 cut(s) 3, 43, 100
NlaIII CATG 4 cut(s) 17, 229, 235, 262
NmuCI GTSAC 1 cut(s) 254
NspI RCATGY 2 cut(s) 235, 262
PagI TCATGA 1 cut(s) 225
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 2 cut(s) 163, 274
PmaCI CACGTG 1 cut(s) 239
PmlI CACGTG 1 cut(s) 239
PpsI GAGTC 2 cut(s) 163, 274
Ppu21I YACGTR 1 cut(s) 239
PspCI CACGTG 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 3 cut(s) 3, 43, 100
SchI GAGTC 2 cut(s) 164, 274
SduI GDGCHC 2 cut(s) 244, 306
SetI ASST 5 cut(s) 74, 84, 123, 206, 241
SfaNI GCATC 2 cut(s) 175, 207
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 1 cut(s) 39
SspMI CTAG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 241
TasI AATT 1 cut(s) 39
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 2 cut(s) 27, 277
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 4 cut(s) 30, 122, 214, 242
TspRI CASTG 2 cut(s) 27, 277
Vha464I CTTAAG 1 cut(s) 83
XceI RCATGY 2 cut(s) 235, 262
XspI CTAG 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.