pycom09g10620

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Forward (+)
8675160 .. 8676776
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGGCAGGTCTTGGTCCTGAAGAACAAGAGGTATCCTATGGTGAGGTTGATGACAATTTTTTCACACCATATGATGAGGTTCATGAAAATTTCGATTATATGGGGTTGCAAGAAAATCTTCTAAAGGGCATTTATGCTTATGGTTTTAAGAAGCCTTGTACAATTCAGAAAAGGGGTATTGTTCCATTCTGCAGAGGTCTTGATGTTATTGAACAGGCTCATCCTGGAACTGGGAAAACAGCAGCTCGCTGCACTGGAATTTTGCAGCGACTTGATTATGGTCTAAACCAGTGCCAGGCATTGGTGTTGACACCTAGGAACGATGTGGCGCTGCAGACGGAGAAGCTTATGCGAGCACTTGGCAAATATCTCGGCATGAGGGTTCTTGCTTGTGTAGGCGGAGCGAGTGTTCGTGAAGATAAACAAATTCTTCAAGCTGGTGTTCATGTTGTCGTTGGCACTCCCGGCTCTGTATTTGACACGTTTCACTGGGATCAGGCACTTTGCAGGGATCATATTAAGATTTTCATACTGGACGAGGCTGATGAATTGCTTACTGGTGGTTTGAAGACCAGATGGAAGCTTGAGAAAATTTCTGAGCTCTATAACTCCTCTATCACTCAAACTGTCATTTTTGTTAACACAACTGACGTGGTGGAGTGGCTTGCTGACAAGATGCGACACCGCAACCACGCTGTCTCGGCCATCCATGCTGACATGGACTGGTACACTTGTGATGCAATTGTACGTAAATTCCATGCCGGCTCTTGCTGTGTTCTCATCACCGACGATGTCAAGGTGGATTGTATAGATGCGCCGCATCAGCAGCAGCAGCAAGAATCTGTTACAGTAGTAAACCATGATTTGCCGGATGATTTGGAAGATTATGTTCATCGATTGGGACCGAGCTTAAGGTTTAGAAGAAAAGGTCATGTCATTTGCTTTGTGGCGAGGGAGGAGGAGAGAAAGCTGCATGACATTCAAAGACTTTACAATGTGGACATCAAGGAGCTGCTAAAATTGTACCCTCATGTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

347

Amino Acids

39.31

Weight (kDa)

5.83

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 54 - 213 1.2e-25 DEAD/DEAH box helicase
Helicase_C PF00271 194 - 305 2.1e-10 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 301
AciI CCGC 3 cut(s) 399, 685, 818
AclWI GGATC 2 cut(s) 501, 519
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 5 cut(s) 88, 258, 426, 591, 752
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 4 cut(s) 160, 728, 747, 1025
AfiI CCNNNNNNNGG 3 cut(s) 230, 295, 301
AflII CTTAAG 1 cut(s) 910
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 4 cut(s) 212, 434, 568, 983
AjiI CACGTC 1 cut(s) 652
AjnI CCWGG 2 cut(s) 223, 294
AloI GAACNNNNNNTCC 2 cut(s) 393, 425
AluBI AGCT 8 cut(s) 245, 346, 437, 583, 601, 909, 970, 1012
AluI AGCT 8 cut(s) 245, 346, 437, 583, 601, 909, 970, 1012
Alw21I GWGCWC 2 cut(s) 358, 603
Alw26I GTCTC 1 cut(s) 703
AlwI GGATC 2 cut(s) 501, 519
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 9 cut(s) 242, 249, 265, 331, 826, 829, 832, 970, 1012
ApoI RAATTY 5 cut(s) 88, 258, 426, 591, 752
Asp700I GAANNNNTTC 1 cut(s) 117
AspA2I CCTAGG 1 cut(s) 314
AspLEI GCGC 2 cut(s) 331, 817
AspS9I GGNCC 2 cut(s) 14, 902
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 2 cut(s) 53, 775
AvaII GGWCC 2 cut(s) 14, 902
AvrII CCTAGG 1 cut(s) 314
BaeI ACNNNNGTAYC 2 cut(s) 718, 751
BanII GRGCYC 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 2 cut(s) 358, 603
BbvI GCAGC 9 cut(s) 236, 254, 277, 318, 838, 841, 844, 957, 999
BccI CCATC 2 cut(s) 570, 713
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BciT130I CCWGG 2 cut(s) 225, 296
BciVI GTATCC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 703
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 2 cut(s) 190, 332
BfoI RGCGCY 1 cut(s) 332
BfrI CTTAAG 1 cut(s) 910
BfuI GTATCC 1 cut(s) 43
BlnI CCTAGG 1 cut(s) 314
Bme1390I CCNGG 3 cut(s) 225, 296, 465
Bme18I GGWCC 2 cut(s) 14, 902
BmgBI CACGTC 1 cut(s) 652
BmgT120I GGNCC 2 cut(s) 14, 902
BmiI GGNNCC 1 cut(s) 903
BmrFI CCNGG 3 cut(s) 225, 296, 465
BmrI ACTGGG 2 cut(s) 240, 499
BmsI GCATC 4 cut(s) 666, 727, 802, 829
BmuI ACTGGG 2 cut(s) 240, 499
BpiI GAAGAC 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 465
Bsa29I ATCGAT 1 cut(s) 895
BsaAI YACGTR 1 cut(s) 749
BsaJI CCNNGG 1 cut(s) 314
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 295, 301
Bse118I RCCGGY 1 cut(s) 761
Bse1I ACTGG 7 cut(s) 235, 259, 289, 494, 537, 562, 728
BseBI CCWGG 2 cut(s) 225, 296
BseCI ATCGAT 1 cut(s) 895
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 3 cut(s) 220, 705, 877
BseLI CCNNNNNNNGG 3 cut(s) 230, 295, 301
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 7 cut(s) 235, 259, 289, 494, 537, 562, 728
BseRI GAGGAG 3 cut(s) 601, 971, 974
BseXI GCAGC 9 cut(s) 236, 254, 277, 318, 838, 841, 844, 957, 999
BsgI GTGCAG 1 cut(s) 235
BshFI GGCC 1 cut(s) 704
BshVI ATCGAT 1 cut(s) 895
BsiHKAI GWGCWC 2 cut(s) 358, 603
BsiSI CCGG 3 cut(s) 465, 762, 869
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 3 cut(s) 230, 295, 301
BsmAI GTCTC 1 cut(s) 703
BsmFI GGGAC 1 cut(s) 915
BsnI GGCC 1 cut(s) 704
Bsp1286I GDGCHC 2 cut(s) 358, 603
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 2 cut(s) 493, 511
BspACI CCGC 3 cut(s) 399, 685, 818
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 1 cut(s) 589
BspDI ATCGAT 1 cut(s) 895
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 903
BspMAI CTGCAG 2 cut(s) 194, 336
BspPI GGATC 2 cut(s) 501, 519
BspTI CTTAAG 1 cut(s) 910
BsrFI RCCGGY 1 cut(s) 761
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 7 cut(s) 235, 259, 289, 494, 537, 562, 728
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 2 cut(s) 493, 511
BssT1I CCWWGG 1 cut(s) 314
Bst2UI CCWGG 2 cut(s) 225, 296
Bst4CI ACNGT 2 cut(s) 628, 850
BstAFI CTTAAG 1 cut(s) 910
BstAUI TGTACA 1 cut(s) 158
BstBAI YACGTR 1 cut(s) 749
BstC8I GCNNGC 4 cut(s) 247, 354, 666, 763
BstDEI CTNAG 1 cut(s) 597
BstF5I GGATG 3 cut(s) 220, 705, 877
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 2 cut(s) 331, 817
BstKTI GATC 2 cut(s) 496, 514
BstMAI GTCTC 1 cut(s) 703
BstMBI GATC 2 cut(s) 493, 511
BstMWI GCNNNNNNNGC 6 cut(s) 465, 701, 710, 823, 826, 832
BstNI CCWGG 2 cut(s) 225, 296
BstSCI CCNGG 3 cut(s) 223, 294, 463
BstSFI CTRYAG 2 cut(s) 190, 332
BstSNI TACGTA 1 cut(s) 749
BstV1I GCAGC 9 cut(s) 236, 254, 277, 318, 838, 841, 844, 957, 999
BstV2I GAAGAC 1 cut(s) 575
Bsu15I ATCGAT 1 cut(s) 895
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 1 cut(s) 704
BsuTUI ATCGAT 1 cut(s) 895
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 3 cut(s) 220, 705, 877
BtsIMutI CAGTG 3 cut(s) 252, 296, 487
Cac8I GCNNGC 4 cut(s) 247, 354, 666, 763
CciI TCATGA 1 cut(s) 82
CfoI GCGC 2 cut(s) 331, 817
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 2 cut(s) 14, 902
ClaI ATCGAT 1 cut(s) 895
Csp6I GTAC 4 cut(s) 159, 727, 746, 1024
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviQI GTAC 4 cut(s) 159, 727, 746, 1024
DdeI CTNAG 1 cut(s) 597
DpnI GATC 2 cut(s) 495, 513
DpnII GATC 2 cut(s) 493, 511
EaeI YGGCCR 1 cut(s) 702
EciI GGCGGA 1 cut(s) 414
Ecl136II GAGCTC 1 cut(s) 601
Eco105I TACGTA 1 cut(s) 749
Eco130I CCWWGG 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 603
Eco47I GGWCC 2 cut(s) 14, 902
Eco53kI GAGCTC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 39
EcoICRI GAGCTC 1 cut(s) 601
EcoRII CCWGG 2 cut(s) 223, 294
EcoT14I CCWWGG 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 314
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FaqI GGGAC 1 cut(s) 915
FauNDI CATATG 1 cut(s) 70
FokI GGATG 3 cut(s) 207, 692, 884
FriOI GRGCYC 1 cut(s) 603
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 330, 816
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 1 cut(s) 704
HapII CCGG 3 cut(s) 465, 762, 869
HhaI GCGC 2 cut(s) 331, 817
Hin6I GCGC 2 cut(s) 329, 815
HinP1I GCGC 2 cut(s) 329, 815
HincII GTYRAC 2 cut(s) 309, 640
HindII GTYRAC 2 cut(s) 309, 640
HindIII AAGCTT 2 cut(s) 344, 581
HinfI GANTC 1 cut(s) 839
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 3 cut(s) 465, 762, 869
HphI GGTGA 2 cut(s) 53, 775
Hpy166II GTNNAC 5 cut(s) 309, 640, 729, 856, 1000
Hpy188I TCNGA 3 cut(s) 168, 598, 1040
Hpy188III TCNNGA 4 cut(s) 17, 83, 200, 413
Hpy8I GTNNAC 5 cut(s) 309, 640, 729, 856, 1000
Hpy99I CGWCG 1 cut(s) 791
HpyCH4III ACNGT 2 cut(s) 628, 850
HpyCH4IV ACGT 3 cut(s) 482, 651, 748
HpyCH4V TGCA 8 cut(s) 109, 192, 252, 265, 334, 507, 740, 973
HpyF10VI GCNNNNNNNGC 6 cut(s) 465, 701, 710, 823, 826, 832
HpyF3I CTNAG 1 cut(s) 597
HpySE526I ACGT 3 cut(s) 482, 651, 748
HspAI GCGC 2 cut(s) 329, 815
KroI GCCGGC 1 cut(s) 761
KroNI GCCGGC 1 cut(s) 763
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 2 cut(s) 493, 511
LmnI GCTCC 2 cut(s) 401, 1009
Lsp1109I GCAGC 9 cut(s) 236, 254, 277, 318, 838, 841, 844, 957, 999
LweI GCATC 4 cut(s) 666, 727, 802, 829
MaeI CTAG 1 cut(s) 315
MaeII ACGT 3 cut(s) 482, 651, 748
MaeIII GTNAC 1 cut(s) 844
MalI GATC 2 cut(s) 495, 513
MboI GATC 2 cut(s) 493, 511
MboII GAAGA 7 cut(s) 32, 110, 422, 428, 580, 893, 933
MfeI CAATTG 1 cut(s) 741
MhlI GDGCHC 2 cut(s) 358, 603
MroNI GCCGGC 1 cut(s) 761
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 4 cut(s) 147, 519, 639, 911
MspCI CTTAAG 1 cut(s) 910
MspI CCGG 3 cut(s) 465, 762, 869
MspR9I CCNGG 3 cut(s) 225, 296, 465
MunI CAATTG 1 cut(s) 741
MvaI CCWGG 2 cut(s) 225, 296
MwoI GCNNNNNNNGC 6 cut(s) 465, 701, 710, 823, 826, 832
NaeI GCCGGC 1 cut(s) 763
NciI CCSGG 1 cut(s) 465
NdeI CATATG 1 cut(s) 70
NdeII GATC 2 cut(s) 493, 511
NgoMIV GCCGGC 1 cut(s) 761
NlaIV GGNNCC 1 cut(s) 903
NmeAIII GCCGAG 2 cut(s) 351, 680
PagI TCATGA 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 763
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 839
PflFI GACNNNGTC 1 cut(s) 791
PflMI CCANNNNNTGG 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 603
Psp6I CCWGG 2 cut(s) 223, 294
PspGI CCWGG 2 cut(s) 223, 294
PspN4I GGNNCC 1 cut(s) 903
PspPI GGNCC 2 cut(s) 14, 902
PstI CTGCAG 2 cut(s) 194, 336
PsyI GACNNNGTC 1 cut(s) 791
RsaI GTAC 4 cut(s) 160, 728, 747, 1025
RsaNI GTAC 4 cut(s) 159, 727, 746, 1024
SacI GAGCTC 1 cut(s) 603
SaqAI TTAA 4 cut(s) 147, 519, 639, 911
Sau3AI GATC 2 cut(s) 493, 511
Sau96I GGNCC 2 cut(s) 14, 902
ScrFI CCNGG 3 cut(s) 225, 296, 465
SduI GDGCHC 2 cut(s) 358, 603
SfaNI GCATC 4 cut(s) 666, 727, 802, 829
SfcI CTRYAG 2 cut(s) 190, 332
SinI GGWCC 2 cut(s) 14, 902
SmlI CTYRAG 2 cut(s) 584, 910
SmoI CTYRAG 2 cut(s) 584, 910
SnaBI TACGTA 1 cut(s) 749
SsiI CCGC 3 cut(s) 399, 685, 818
SspMI CTAG 1 cut(s) 315
SstI GAGCTC 1 cut(s) 603
StyD4I CCNGG 3 cut(s) 223, 294, 463
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 2 cut(s) 628, 850
TaiI ACGT 3 cut(s) 485, 654, 751
TaqI TCGA 2 cut(s) 93, 895
TaqII GACCGA 1 cut(s) 919
TatI WGTACW 1 cut(s) 158
TauI GCSGC 1 cut(s) 820
TfiI GAWTC 1 cut(s) 839
Tru1I TTAA 4 cut(s) 147, 519, 639, 911
Tru9I TTAA 4 cut(s) 147, 519, 639, 911
TscAI CASTG 3 cut(s) 259, 296, 494
TseI GCWGC 9 cut(s) 242, 249, 265, 331, 826, 829, 832, 970, 1012
TspDTI ATGAA 6 cut(s) 71, 99, 434, 517, 561, 881
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 3 cut(s) 259, 296, 494
Tth111I GACNNNGTC 1 cut(s) 791
Van91I CCANNNNNTGG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 910
VpaK11BI GGWCC 2 cut(s) 14, 902
XapI RAATTY 5 cut(s) 88, 258, 426, 591, 752
XmaJI CCTAGG 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.