Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

9664756 .. 9667398

Gene Structure 2643 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGCTGCATAGACTGGTCAGCACTCAGCAGCACAAAGAATCCATCAGTTTCTTCTCTTATATTTGGTTTTTTCTTCACTCTCTCTTATGCTTTCATCATGAAGTTCTGCATGAAACTCTCTCTCGTGTGTTTGTTGTTCCAGCAACCGTGCTATCAGATTCCTCTCTGAGCTCAAATGGGGTCTCAGATATCCCTAAGAAGTTGCTCAGTTTTGCCCAGAACCCCCAGGTGTTTGATTGGATGGTGGGAATCAGGAGGAAAATACATGCAAACCCAGAACTGGGTTATGAGGAATTTGAGACCAGTGAGCTGATCAGAGCAGAATTGGATCAAATGAGTGTTGAATATAAACACCCAGTTGGGGTTACTGGTGTTGTAGGCTTCATTGGCACCCGAAAACCGCCTTTCGTTGCAATCAGAGCTGATATGGATGCTCTGACCATGCAGGAAATGGTGGAGTGGGAGCACAGGAGTAAAAATGCAGGGAAAATGCATGCTTGTGGGCATGATGCTCACGTTGCAATGCTTCTTGGTGCAGCAAAGATCCTTAAAGACCATGAGAAAGATTTGCAGGGAACAGTTGTTCTTGTATTTCAACCGGCGGAGGAAGGAGGAGCAGGGGCTAAGAAAATGATAGCTGGAAGAGCATTAGAGAATGTTAGCGCTATTTTTGGTTTGCATGTTGCAAACCACGTACCTATTGCTGAAGTGGCCTCCGGACCTGGTCCCTTTTTAGCTGGGGCTGGCTTCTTTGAAGCGACAATAAGTGGAAAAGGTGGTCATGCAGCCCTTCCTCACCACGTGGTTGACCCGATCCTGGCAGCTTCTAATGTGATTGTCAGCTTGCAACATCTTGTCTCGCGTGAAGCTCATCCACTCGACTCGCAGGTAATCACTGTTGGAAGATTTCAAGGAGGTGATGCATTCAATGTTATTCCAGATTCTGTCAAAATAGGTGGTACTTTCCGTGCATTTACAATGGAAAGCCTAATGCAACTCAAGCAGCGGATTAAGCAGGTTATAACAGGACAAGCATCGGTACACAGGTGCCTCGCAACTGTCAATTTCTTTGAAGATGAGAAACCTTTGTCCCCACCGACCATAAGCCATAAGGACTTGCACGAACACTTCAGAAACGTAGCTGGGATATGCTTGGCTTCGAAAAGGTCAAGGACCATCAACCGTGGATGGGATCCGAGGATTTTGCATACTAGCAAGAGGCAATACCTGGATACTTTTCCTTCCTTGGAATGGCAGACGATGCACTTGGACCCCTACCTACGCCACATTCACCTTACTTCTGTATCAACGAAGATGCACTTCCTTATGGTGCTGCACTCCATGCTTCTTTCGCTGCTAGGGATTGCTCGAGTTTCAACAGGAAGTTCCTTTGCCAGAACGAGAACACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006950 GO:0008150 GO:0009611 GO:0010112 GO:0010178 GO:0010179 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0031347 GO:0032101 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0071944 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

471

Amino Acids

52.04

Weight (kDa)

8.58

Isoelectric Point (pI)

36.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	138 - 381	2.5e-29	Peptidase family M20/M25/M40
M20_dimer	PF07687	247 - 342	6.7e-13	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1022
Acc36I	ACCTGC	2 cut(s)	877, 1006
AccB1I	GGYRCC	2 cut(s)	389, 1047
AccII	CGCG	1 cut(s)	862
AccIII	TCCGGA	1 cut(s)	716
AciI	CCGC	3 cut(s)	401, 602, 1006
AclWI	GGATC	5 cut(s)	336, 538, 808, 1187, 1200
AcsI	RAATTY	1 cut(s)	293
AcuI	CTGAAG	2 cut(s)	726, 1114
AcvI	CACGTG	1 cut(s)	802
AdeI	CACNNNGTG	1 cut(s)	802
AfaI	GTAC	3 cut(s)	696, 961, 1041
AfeI	AGCGCT	1 cut(s)	664
AfiI	CCNNNNNNNGG	6 cut(s)	280, 281, 361, 817, 1189, 1251
AgsI	TTSAA	7 cut(s)	344, 596, 755, 911, 928, 1073, 1377
AjnI	CCWGG	4 cut(s)	225, 721, 816, 1227
AloI	GAACNNNNNNTCC	2 cut(s)	567, 599
AluBI	AGCT	9 cut(s)	171, 310, 422, 638, 737, 824, 843, 869, 1142
AluI	AGCT	9 cut(s)	171, 310, 422, 638, 737, 824, 843, 869, 1142
Alw21I	GWGCWC	2 cut(s)	173, 468
Alw26I	GTCTC	3 cut(s)	187, 293, 862
AlwI	GGATC	5 cut(s)	336, 538, 808, 1187, 1200
AlwNI	CAGNNNCTG	1 cut(s)	944
Ama87I	CYCGRG	1 cut(s)	1368
Aor13HI	TCCGGA	1 cut(s)	716
Aor51HI	AGCGCT	1 cut(s)	664
AoxI	GGCC	1 cut(s)	711
ApeKI	GCWGC	8 cut(s)	4, 28, 536, 785, 821, 1003, 1333, 1354
ApoI	RAATTY	1 cut(s)	293
AspLEI	GCGC	1 cut(s)	665
AspS9I	GGNCC	4 cut(s)	719, 725, 1173, 1270
AsuHPI	GGTGA	3 cut(s)	788, 929, 1283
AsuII	TTCGAA	1 cut(s)	1160
AvaI	CYCGRG	1 cut(s)	1368
AvaII	GGWCC	4 cut(s)	719, 725, 1173, 1270
BaeI	ACNNNNGTAYC	4 cut(s)	1031, 1064, 1287, 1320
BamHI	GGATCC	1 cut(s)	1192
BanI	GGYRCC	2 cut(s)	389, 1047
BanII	GRGCYC	1 cut(s)	173
BauI	CACGAG	1 cut(s)	123
BbrPI	CACGTG	1 cut(s)	802
Bbv12I	GWGCWC	2 cut(s)	173, 468
BbvI	GCAGC	7 cut(s)	40, 548, 797, 833, 1015, 1320, 1341
BccI	CCATC	4 cut(s)	50, 235, 1182, 1184
BcgI	CGANNNNNNTGC	2 cut(s)	1186, 1220
BciT130I	CCWGG	4 cut(s)	227, 723, 818, 1229
BciVI	GTATCC	1 cut(s)	1225
BclI	TGATCA	1 cut(s)	312
BcoDI	GTCTC	3 cut(s)	187, 293, 862
BfaI	CTAG	2 cut(s)	1212, 1358
BfoI	RGCGCY	1 cut(s)	666
BfuAI	ACCTGC	2 cut(s)	877, 1006
BfuI	GTATCC	1 cut(s)	1225
BisI	GCNGC	8 cut(s)	5, 29, 537, 786, 822, 1004, 1334, 1355
BlsI	GCNGC	8 cut(s)	6, 30, 538, 787, 823, 1005, 1335, 1356
Bme1390I	CCNGG	4 cut(s)	227, 723, 818, 1229
Bme18I	GGWCC	4 cut(s)	719, 725, 1173, 1270
BmeT110I	CYCGRG	1 cut(s)	1368
BmgT120I	GGNCC	4 cut(s)	719, 725, 1173, 1270
BmiI	GGNNCC	5 cut(s)	391, 727, 1049, 1194, 1272
BmrFI	CCNGG	4 cut(s)	227, 723, 818, 1229
BmrI	ACTGGG	2 cut(s)	290, 350
BmsI	GCATC	6 cut(s)	421, 499, 910, 1043, 1251, 1305
BmuI	ACTGGG	2 cut(s)	290, 350
Bpu14I	TTCGAA	1 cut(s)	1160
BpuEI	CTTGAG	1 cut(s)	983
BsaAI	YACGTR	2 cut(s)	694, 802
BsaI	GGTCTC	2 cut(s)	187, 293
BsaJI	CCNNGG	4 cut(s)	225, 1183, 1196, 1245
BsaWI	WCCGGW	1 cut(s)	716
Bsc4I	CCNNNNNNNGG	6 cut(s)	280, 281, 361, 817, 1189, 1251
Bse118I	RCCGGY	1 cut(s)	598
Bse1I	ACTGG	5 cut(s)	18, 285, 303, 356, 373
Bse3DI	GCAATG	1 cut(s)	528
BseAI	TCCGGA	1 cut(s)	716
BseBI	CCWGG	4 cut(s)	227, 723, 818, 1229
BseDI	CCNNGG	4 cut(s)	225, 1183, 1196, 1245
BseGI	GGATG	4 cut(s)	246, 436, 871, 1193
BseLI	CCNNNNNNNGG	6 cut(s)	280, 281, 361, 817, 1189, 1251
BseMI	GCAATG	1 cut(s)	528
BseMII	CTCAG	4 cut(s)	38, 158, 198, 220
BseNI	ACTGG	5 cut(s)	18, 285, 303, 356, 373
BseRI	GAGGAG	1 cut(s)	627
BseXI	GCAGC	7 cut(s)	40, 548, 797, 833, 1015, 1320, 1341
BseYI	CCCAGC	2 cut(s)	737, 1142
BsgI	GTGCAG	2 cut(s)	555, 1319
Bsh1236I	CGCG	1 cut(s)	862
BshFI	GGCC	1 cut(s)	713
BshNI	GGYRCC	2 cut(s)	389, 1047
BsiHKAI	GWGCWC	2 cut(s)	173, 468
BsiHKCI	CYCGRG	1 cut(s)	1368
BsiSI	CCGG	2 cut(s)	599, 717
BslFI	GGGAC	2 cut(s)	711, 1075
BslI	CCNNNNNNNGG	6 cut(s)	280, 281, 361, 817, 1189, 1251
BsmAI	GTCTC	3 cut(s)	187, 293, 862
BsmFI	GGGAC	2 cut(s)	711, 1075
BsmI	GAATGC	1 cut(s)	923
BsnI	GGCC	1 cut(s)	713
Bso31I	GGTCTC	2 cut(s)	187, 293
BsoBI	CYCGRG	1 cut(s)	1368
Bsp119I	TTCGAA	1 cut(s)	1160
Bsp1286I	GDGCHC	2 cut(s)	173, 468
Bsp13I	TCCGGA	1 cut(s)	716
Bsp143I	GATC	5 cut(s)	312, 328, 543, 813, 1192
BspACI	CCGC	3 cut(s)	401, 602, 1006
BspANI	GGCC	1 cut(s)	713
BspCNI	CTCAG	4 cut(s)	37, 159, 197, 219
BspEI	TCCGGA	1 cut(s)	716
BspFNI	CGCG	1 cut(s)	862
BspHI	TCATGA	1 cut(s)	97
BspLI	GGNNCC	5 cut(s)	391, 727, 1049, 1194, 1272
BspMI	ACCTGC	2 cut(s)	877, 1006
BspPI	GGATC	5 cut(s)	336, 538, 808, 1187, 1200
BspQI	GCTCTTC	1 cut(s)	637
BspT104I	TTCGAA	1 cut(s)	1160
BspT107I	GGYRCC	2 cut(s)	389, 1047
BspTNI	GGTCTC	2 cut(s)	187, 293
BsrDI	GCAATG	1 cut(s)	528
BsrFI	RCCGGY	1 cut(s)	598
BsrI	ACTGG	5 cut(s)	18, 285, 303, 356, 373
BssAI	RCCGGY	1 cut(s)	598
BssECI	CCNNGG	4 cut(s)	225, 1183, 1196, 1245
BssMI	GATC	5 cut(s)	312, 328, 543, 813, 1192
BssSI	CACGAG	1 cut(s)	123
BssT1I	CCWWGG	1 cut(s)	1245
Bst2BI	CACGAG	1 cut(s)	123
Bst2UI	CCWGG	4 cut(s)	227, 723, 818, 1229
Bst4CI	ACNGT	5 cut(s)	148, 580, 898, 1060, 1184
Bst6I	CTCTTC	1 cut(s)	637
BstAPI	GCANNNNNTGC	2 cut(s)	1261, 1342
BstBAI	YACGTR	2 cut(s)	694, 802
BstBI	TTCGAA	1 cut(s)	1160
BstC8I	GCNNGC	3 cut(s)	495, 745, 845
BstDEI	CTNAG	6 cut(s)	24, 167, 184, 195, 206, 624
BstDSI	CCRYGG	1 cut(s)	1183
BstF5I	GGATG	4 cut(s)	246, 436, 871, 1193
BstFNI	CGCG	1 cut(s)	862
BstH2I	RGCGCY	1 cut(s)	666
BstHHI	GCGC	1 cut(s)	665
BstKTI	GATC	5 cut(s)	315, 331, 546, 816, 1195
BstMAI	GTCTC	3 cut(s)	187, 293, 862
BstMBI	GATC	5 cut(s)	312, 328, 543, 813, 1192
BstNI	CCWGG	4 cut(s)	227, 723, 818, 1229
BstNSI	RCATGY	3 cut(s)	269, 497, 683
BstSCI	CCNGG	4 cut(s)	225, 721, 816, 1227
BstUI	CGCG	1 cut(s)	862
BstV1I	GCAGC	7 cut(s)	40, 548, 797, 833, 1015, 1320, 1341
BstX2I	RGATCY	2 cut(s)	543, 1192
BstYI	RGATCY	2 cut(s)	543, 1192
BsuI	GTATCC	1 cut(s)	1225
BsuRI	GGCC	1 cut(s)	713
BtgI	CCRYGG	1 cut(s)	1183
BtsCI	GGATG	4 cut(s)	246, 436, 871, 1193
BtsIMutI	CAGTG	2 cut(s)	310, 894
BveI	ACCTGC	2 cut(s)	877, 1006
Cac8I	GCNNGC	3 cut(s)	495, 745, 845
CaiI	CAGNNNCTG	1 cut(s)	944
CciI	TCATGA	1 cut(s)	97
CfoI	GCGC	1 cut(s)	665
Cfr10I	RCCGGY	1 cut(s)	598
Cfr13I	GGNCC	4 cut(s)	719, 725, 1173, 1270
CsiI	ACCWGGT	1 cut(s)	721
Csp6I	GTAC	3 cut(s)	695, 960, 1040
CspCI	CAANNNNNGTGG	2 cut(s)	937, 972
CviQI	GTAC	3 cut(s)	695, 960, 1040
DdeI	CTNAG	6 cut(s)	24, 167, 184, 195, 206, 624
DpnI	GATC	5 cut(s)	314, 330, 545, 815, 1194
DpnII	GATC	5 cut(s)	312, 328, 543, 813, 1192
DraIII	CACNNNGTG	1 cut(s)	802
Eam1104I	CTCTTC	1 cut(s)	637
EarI	CTCTTC	1 cut(s)	637
EciI	GGCGGA	1 cut(s)	617
Ecl136II	GAGCTC	1 cut(s)	171
Eco130I	CCWWGG	1 cut(s)	1245
Eco24I	GRGCYC	1 cut(s)	173
Eco31I	GGTCTC	2 cut(s)	187, 293
Eco32I	GATATC	1 cut(s)	190
Eco47I	GGWCC	4 cut(s)	719, 725, 1173, 1270
Eco47III	AGCGCT	1 cut(s)	664
Eco53kI	GAGCTC	1 cut(s)	171
Eco57I	CTGAAG	2 cut(s)	726, 1114
Eco72I	CACGTG	1 cut(s)	802
Eco88I	CYCGRG	1 cut(s)	1368
EcoICRI	GAGCTC	1 cut(s)	171
EcoRII	CCWGG	4 cut(s)	225, 721, 816, 1227
EcoRV	GATATC	1 cut(s)	190
EcoT14I	CCWWGG	1 cut(s)	1245
EcoT22I	ATGCAT	2 cut(s)	495, 925
EcoT38I	GRGCYC	1 cut(s)	173
ErhI	CCWWGG	1 cut(s)	1245
FalI	AAGNNNNNCTT	2 cut(s)	1304, 1336
FaqI	GGGAC	2 cut(s)	711, 1075
FbaI	TGATCA	1 cut(s)	312
Fnu4HI	GCNGC	8 cut(s)	5, 29, 537, 786, 822, 1004, 1334, 1355
FokI	GGATG	4 cut(s)	253, 443, 858, 1200
FriOI	GRGCYC	1 cut(s)	173
Fsp4HI	GCNGC	8 cut(s)	5, 29, 537, 786, 822, 1004, 1334, 1355
FspBI	CTAG	2 cut(s)	1212, 1358
GlaI	GCGC	1 cut(s)	664
GluI	GCNGC	8 cut(s)	5, 29, 537, 786, 822, 1004, 1334, 1355
GsaI	CCCAGC	2 cut(s)	741, 1146
HaeII	RGCGCY	1 cut(s)	666
HaeIII	GGCC	1 cut(s)	713
HapII	CCGG	2 cut(s)	599, 717
HhaI	GCGC	1 cut(s)	665
Hin6I	GCGC	1 cut(s)	663
HinP1I	GCGC	1 cut(s)	663
HincII	GTYRAC	1 cut(s)	808
HindII	GTYRAC	1 cut(s)	808
HinfI	GANTC	5 cut(s)	38, 158, 249, 881, 941
HpaII	CCGG	2 cut(s)	599, 717
HphI	GGTGA	3 cut(s)	788, 929, 1283
Hpy166II	GTNNAC	2 cut(s)	808, 1042
Hpy188I	TCNGA	8 cut(s)	157, 168, 187, 317, 419, 438, 1133, 1197
Hpy188III	TCNNGA	4 cut(s)	98, 253, 717, 938
Hpy8I	GTNNAC	2 cut(s)	808, 1042
HpyAV	CCTTC	3 cut(s)	602, 800, 1251
HpyCH4III	ACNGT	5 cut(s)	148, 580, 898, 1060, 1184
HpyCH4IV	ACGT	4 cut(s)	516, 693, 801, 1137
HpyF3I	CTNAG	6 cut(s)	24, 167, 184, 195, 206, 624
HpySE526I	ACGT	4 cut(s)	516, 693, 801, 1137
HspAI	GCGC	1 cut(s)	663
Kpn2I	TCCGGA	1 cut(s)	716
Ksp22I	TGATCA	1 cut(s)	312
Kzo9I	GATC	5 cut(s)	312, 328, 543, 813, 1192
LguI	GCTCTTC	1 cut(s)	637
LmnI	GCTCC	2 cut(s)	463, 614
Lsp1109I	GCAGC	7 cut(s)	40, 548, 797, 833, 1015, 1320, 1341
LweI	GCATC	6 cut(s)	421, 499, 910, 1043, 1251, 1305
MabI	ACCWGGT	1 cut(s)	721
MaeI	CTAG	2 cut(s)	1212, 1358
MaeII	ACGT	4 cut(s)	516, 693, 801, 1137
MaeIII	GTNAC	1 cut(s)	364
MalI	GATC	5 cut(s)	314, 330, 545, 815, 1194
MboI	GATC	5 cut(s)	312, 328, 543, 813, 1192
MboII	GAAGA	6 cut(s)	43, 65, 654, 915, 1085, 1324
MflI	RGATCY	2 cut(s)	543, 1192
MhlI	GDGCHC	2 cut(s)	173, 468
MluCI	AATT	3 cut(s)	293, 323, 1063
MlyI	GAGTC	1 cut(s)	875
MmeI	TCCRAC	1 cut(s)	880
Mph1103I	ATGCAT	2 cut(s)	495, 925
MroI	TCCGGA	1 cut(s)	716
MseI	TTAA	2 cut(s)	549, 1011
MslI	CAYNNNNRTG	1 cut(s)	498
MspA1I	CMGCKG	1 cut(s)	1006
MspI	CCGG	2 cut(s)	599, 717
MspR9I	CCNGG	4 cut(s)	227, 723, 818, 1229
Mva1269I	GAATGC	1 cut(s)	923
MvaI	CCWGG	4 cut(s)	227, 723, 818, 1229
MvnI	CGCG	1 cut(s)	862
NdeII	GATC	5 cut(s)	312, 328, 543, 813, 1192
NlaIV	GGNNCC	5 cut(s)	391, 727, 1049, 1194, 1272
NsiI	ATGCAT	2 cut(s)	495, 925
NspI	RCATGY	3 cut(s)	269, 497, 683
NspV	TTCGAA	1 cut(s)	1160
PaeI	GCATGC	1 cut(s)	497
PaeR7I	CTCGAG	1 cut(s)	1368
PagI	TCATGA	1 cut(s)	97
PciSI	GCTCTTC	1 cut(s)	637
PctI	GAATGC	1 cut(s)	923
PfeI	GAWTC	4 cut(s)	38, 158, 249, 941
PflFI	GACNNNGTC	1 cut(s)	723
PkrI	GCNGC	8 cut(s)	6, 30, 538, 787, 823, 1005, 1335, 1356
PleI	GAGTC	1 cut(s)	875
PmaCI	CACGTG	1 cut(s)	802
PmlI	CACGTG	1 cut(s)	802
PpsI	GAGTC	1 cut(s)	875
Ppu21I	YACGTR	2 cut(s)	694, 802
PsiI	TTATAA	1 cut(s)	1022
Psp124BI	GAGCTC	1 cut(s)	173
Psp6I	CCWGG	4 cut(s)	225, 721, 816, 1227
PspCI	CACGTG	1 cut(s)	802
PspFI	CCCAGC	2 cut(s)	737, 1142
PspGI	CCWGG	4 cut(s)	225, 721, 816, 1227
PspN4I	GGNNCC	5 cut(s)	391, 727, 1049, 1194, 1272
PspPI	GGNCC	4 cut(s)	719, 725, 1173, 1270
PspXI	VCTCGAGB	1 cut(s)	1368
PstNI	CAGNNNCTG	1 cut(s)	944
PsuI	RGATCY	2 cut(s)	543, 1192
PsyI	GACNNNGTC	1 cut(s)	723
RsaI	GTAC	3 cut(s)	696, 961, 1041
RsaNI	GTAC	3 cut(s)	695, 960, 1040
RseI	CAYNNNNRTG	1 cut(s)	498
SacI	GAGCTC	1 cut(s)	173
SapI	GCTCTTC	1 cut(s)	637
SaqAI	TTAA	2 cut(s)	549, 1011
SatI	GCNGC	8 cut(s)	5, 29, 537, 786, 822, 1004, 1334, 1355
Sau3AI	GATC	5 cut(s)	312, 328, 543, 813, 1192
Sau96I	GGNCC	4 cut(s)	719, 725, 1173, 1270
SchI	GAGTC	1 cut(s)	875
ScrFI	CCNGG	4 cut(s)	227, 723, 818, 1229
SduI	GDGCHC	2 cut(s)	173, 468
SexAI	ACCWGGT	1 cut(s)	721
SfaNI	GCATC	6 cut(s)	421, 499, 910, 1043, 1251, 1305
Sfr274I	CTCGAG	1 cut(s)	1368
SfuI	TTCGAA	1 cut(s)	1160
SinI	GGWCC	4 cut(s)	719, 725, 1173, 1270
SlaI	CTCGAG	1 cut(s)	1368
SmiMI	CAYNNNNRTG	1 cut(s)	498
SmlI	CTYRAG	2 cut(s)	998, 1368
SmoI	CTYRAG	2 cut(s)	998, 1368
SphI	GCATGC	1 cut(s)	497
Sse9I	AATT	3 cut(s)	293, 323, 1063
SsiI	CCGC	3 cut(s)	401, 602, 1006
SspMI	CTAG	2 cut(s)	1212, 1358
SstI	GAGCTC	1 cut(s)	173
StyD4I	CCNGG	4 cut(s)	225, 721, 816, 1227
StyI	CCWWGG	1 cut(s)	1245
TaaI	ACNGT	5 cut(s)	148, 580, 898, 1060, 1184
TaiI	ACGT	4 cut(s)	519, 696, 804, 1140
TaqI	TCGA	3 cut(s)	879, 1160, 1369
TasI	AATT	3 cut(s)	293, 323, 1063
TfiI	GAWTC	4 cut(s)	38, 158, 249, 941
Tru1I	TTAA	2 cut(s)	549, 1011
Tru9I	TTAA	2 cut(s)	549, 1011
TscAI	CASTG	2 cut(s)	310, 901
TseI	GCWGC	8 cut(s)	4, 28, 536, 785, 821, 1003, 1333, 1354
TspDTI	ATGAA	4 cut(s)	83, 114, 126, 373
TspGWI	ACGGA	1 cut(s)	956
TspRI	CASTG	2 cut(s)	310, 901
Tth111I	GACNNNGTC	1 cut(s)	723
VpaK11BI	GGWCC	4 cut(s)	719, 725, 1173, 1270
XapI	RAATTY	1 cut(s)	293
XceI	RCATGY	3 cut(s)	269, 497, 683
XcmI	CCANNNNNNNNNTGG	1 cut(s)	448
XhoI	CTCGAG	1 cut(s)	1368
XspI	CTAG	2 cut(s)	1212, 1358
Zsp2I	ATGCAT	2 cut(s)	495, 925

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.