pycom09g17090

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Reverse (-)
17515725 .. 17516911
1187 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
ATGGTCAGTCTGTTGGTAGTGGTCCCACTGTTCGATCTTGCATCACGTCTAGCAACCTTGAGAGGTGTGGTGTTACACAGTCCCATTTTGTCTGGGCTGAGGGTATTGTACCCTGTCAAACGGACGTACTGGTTTGATATTTACAAGAATATCGACAAAATTTCAATGGTGAGCTGTCCTGTTCTGGTTATACATGGGACAGCAGACGAGGTCGTAGATTCGTCCCATGGGAAGCAACTATGGGTGCTTTGCAAGGAAAAGTATGAGCCTTTATGGCTCAGTGGAGGTGGACACTGCAATCTTGAGCTTTACCCAGAATTTATCAAACATCTAAAGAAATTCGTACACAGCCTCGGAAAGTCAAAATCGACCACAAATGGTTCTAGAACAAGTACAGTAGATTCTGAGAACAAGAACAAACCATCTGAAACCGGTACTTCAGATTCAACTGAAGTGGGTTCTGATCTGACAGAAGTTTCTAGAAACAGTTTAGATAGTCGACTGGAGAAATCTAAAAAGTCGAACAAGCCCGAAAAGTCTCGGATGAGCACTGACCGAGTCGACACGTTTAGGAGAAAGAAGGGGTTAGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1 Hydrolase_4

Protein Analysis

199

Amino Acids

22.25

Weight (kDa)

9.47

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 499, 561
AcsI RAATTY 3 cut(s) 159, 317, 338
AcuI CTGAAG 2 cut(s) 423, 471
AfaI GTAC 5 cut(s) 110, 128, 345, 394, 436
AflIII ACRYGT 1 cut(s) 564
AgeI ACCGGT 1 cut(s) 431
AgsI TTSAA 2 cut(s) 165, 447
AjiI CACGTC 1 cut(s) 47
AluBI AGCT 2 cut(s) 174, 307
AluI AGCT 2 cut(s) 174, 307
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 1 cut(s) 543
ApoI RAATTY 3 cut(s) 159, 317, 338
AsiGI ACCGGT 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 551
BbvCI CCTCAGC 1 cut(s) 98
BccI CCATC 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 543
BfaI CTAG 3 cut(s) 50, 384, 480
BglI GCCNNNNNGGC 1 cut(s) 274
Bme18I GGWCC 1 cut(s) 22
BmgBI CACGTC 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 24
BmsI GCATC 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 524
Bpu10I CCTNAGC 1 cut(s) 98
BpuEI CTTGAG 2 cut(s) 79, 323
BsaJI CCNNGG 2 cut(s) 226, 352
BsaWI WCCGGW 1 cut(s) 431
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 134, 507
BseDI CCNNGG 2 cut(s) 226, 352
BseGI GGATG 1 cut(s) 549
BseMII CTCAG 3 cut(s) 89, 292, 396
BseNI ACTGG 2 cut(s) 134, 507
BshTI ACCGGT 1 cut(s) 431
BsiHKAI GWGCWC 1 cut(s) 551
BsiSI CCGG 1 cut(s) 432
BslFI GGGAC 4 cut(s) 8, 66, 208, 211
BsmAI GTCTC 1 cut(s) 543
BsmFI GGGAC 4 cut(s) 8, 66, 208, 211
Bsp1286I GDGCHC 1 cut(s) 551
Bsp143I GATC 2 cut(s) 34, 463
Bsp19I CCATGG 1 cut(s) 226
BspCNI CTCAG 3 cut(s) 90, 291, 397
BspLI GGNNCC 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 2 cut(s) 134, 507
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 226, 352
BssMI GATC 2 cut(s) 34, 463
BssT1I CCWWGG 1 cut(s) 226
Bst4CI ACNGT 4 cut(s) 30, 80, 397, 488
BstDEI CTNAG 3 cut(s) 98, 278, 405
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 549
BstKTI GATC 2 cut(s) 37, 466
BstMAI GTCTC 1 cut(s) 543
BstMBI GATC 2 cut(s) 34, 463
BstMWI GCNNNNNNNGC 1 cut(s) 274
BtgI CCRYGG 1 cut(s) 226
BtrI CACGTC 1 cut(s) 47
BtsCI GGATG 1 cut(s) 549
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 4 cut(s) 26, 286, 292, 549
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 22
Csp6I GTAC 5 cut(s) 109, 127, 344, 393, 435
CspAI ACCGGT 1 cut(s) 431
CspCI CAANNNNNGTGG 2 cut(s) 435, 470
CviAII CATG 2 cut(s) 194, 227
CviJI RGCY 7 cut(s) 97, 174, 268, 277, 307, 351, 529
CviKI_1 RGCY 7 cut(s) 97, 174, 268, 277, 307, 351, 529
CviQI GTAC 5 cut(s) 109, 127, 344, 393, 435
DdeI CTNAG 3 cut(s) 98, 278, 405
DpnI GATC 2 cut(s) 36, 465
DpnII GATC 2 cut(s) 34, 463
Eco130I CCWWGG 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 423, 471
EcoT14I CCWWGG 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 2 cut(s) 197, 230
FaiI YATR 6 cut(s) 191, 195, 228, 241, 264, 274
FaqI GGGAC 4 cut(s) 8, 66, 208, 211
FatI CATG 2 cut(s) 193, 226
FblI GTMKAC 2 cut(s) 499, 561
FokI GGATG 1 cut(s) 556
FspBI CTAG 3 cut(s) 50, 384, 480
GsuI CTGGAG 1 cut(s) 524
HapII CCGG 1 cut(s) 432
Hin1II CATG 2 cut(s) 197, 230
HincII GTYRAC 2 cut(s) 500, 562
HindII GTYRAC 2 cut(s) 500, 562
HinfI GANTC 4 cut(s) 218, 401, 443, 558
HpaII CCGG 1 cut(s) 432
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 4 cut(s) 290, 346, 500, 562
Hpy188I TCNGA 7 cut(s) 356, 406, 427, 442, 463, 468, 543
Hpy188III TCNNGA 3 cut(s) 302, 384, 480
Hpy8I GTNNAC 4 cut(s) 290, 346, 500, 562
HpyAV CCTTC 1 cut(s) 574
HpyCH4III ACNGT 4 cut(s) 30, 80, 397, 488
HpyCH4IV ACGT 3 cut(s) 46, 125, 566
HpyCH4V TGCA 3 cut(s) 41, 252, 297
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpyF3I CTNAG 3 cut(s) 98, 278, 405
HpySE526I ACGT 3 cut(s) 46, 125, 566
Hsp92II CATG 2 cut(s) 197, 230
Kzo9I GATC 2 cut(s) 34, 463
LpnPI CCDG 8 cut(s) 78, 115, 126, 170, 192, 327, 445, 488
LweI GCATC 1 cut(s) 50
MaeI CTAG 3 cut(s) 50, 384, 480
MaeII ACGT 3 cut(s) 46, 125, 566
MaeIII GTNAC 1 cut(s) 72
MalI GATC 2 cut(s) 36, 465
MboI GATC 2 cut(s) 34, 463
MhlI GDGCHC 1 cut(s) 551
MluCI AATT 3 cut(s) 159, 317, 338
MlyI GAGTC 1 cut(s) 567
MnlI CCTC 5 cut(s) 56, 93, 202, 278, 362
MspI CCGG 1 cut(s) 432
MwoI GCNNNNNNNGC 1 cut(s) 274
NcoI CCATGG 1 cut(s) 226
NdeII GATC 2 cut(s) 34, 463
NlaIII CATG 2 cut(s) 197, 230
NlaIV GGNNCC 1 cut(s) 24
PfeI GAWTC 3 cut(s) 218, 401, 443
PflFI GACNNNGTC 2 cut(s) 209, 557
PinAI ACCGGT 1 cut(s) 431
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
PspN4I GGNNCC 1 cut(s) 24
PspPI GGNCC 1 cut(s) 22
PsyI GACNNNGTC 2 cut(s) 209, 557
RsaI GTAC 5 cut(s) 110, 128, 345, 394, 436
RsaNI GTAC 5 cut(s) 109, 127, 344, 393, 435
SalI GTCGAC 2 cut(s) 498, 560
Sau3AI GATC 2 cut(s) 34, 463
Sau96I GGNCC 1 cut(s) 22
SchI GAGTC 1 cut(s) 567
SduI GDGCHC 1 cut(s) 551
SetI ASST 9 cut(s) 49, 59, 67, 128, 176, 213, 289, 309, 569
SfaNI GCATC 1 cut(s) 50
SinI GGWCC 1 cut(s) 22
SmlI CTYRAG 2 cut(s) 58, 302
SmoI CTYRAG 2 cut(s) 58, 302
Sse9I AATT 3 cut(s) 159, 317, 338
SspMI CTAG 3 cut(s) 50, 384, 480
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 4 cut(s) 30, 80, 397, 488
TaiI ACGT 3 cut(s) 49, 128, 569
TaqI TCGA 6 cut(s) 33, 153, 368, 499, 521, 561
TaqII GACCGA 1 cut(s) 570
TasI AATT 3 cut(s) 159, 317, 338
TatI WGTACW 1 cut(s) 392
TfiI GAWTC 3 cut(s) 218, 401, 443
TscAI CASTG 4 cut(s) 33, 286, 299, 556
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 4 cut(s) 33, 286, 299, 556
Tth111I GACNNNGTC 2 cut(s) 209, 557
VpaK11BI GGWCC 1 cut(s) 22
XapI RAATTY 3 cut(s) 159, 317, 338
XbaI TCTAGA 2 cut(s) 383, 479
XmiI GTMKAC 2 cut(s) 499, 561
XspI CTAG 3 cut(s) 50, 384, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.