pycom09g17190

LisH domain and HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Reverse (-)
17587818 .. 17588677
860 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
ATGCTCCAAGAAAATGATTCTTTATCGAAAGAAAAGGAGAGTCTATATCTTGAAAAGCTATCCTTACTTAAAAACAAAGATTTGGCTGAAGGCCAAATAAGTACACTAATCAAATCAATGGAAGCTCTTCAGAAGGATCTTAAAGACAAAGAAAGCCTTGTACAAGATCTGAAGAAGTCCTTGGAACACCAGAGAAAGGAGCTCAATGATTGCAGAGCTGAAGTTACTGCATTGAAAATGCACATTGAAGGATATCGTTCTGGACGGAATATGGTAGCTGCTGAAACTGATCAAATCCAACCTCTATCCTTGGAAAAATACAAGGAAGAAATTAAATCACTACAGATGGAACTAGAAAGTTTTAAATCCAAAAACACAAAAGCTCATGATTATTCAGATTCCACCAATTTTGTAAAAGAGTCTGTGCAAATGGAAGAAAAGGTCGTTGTCGTGGATGAAGATAAAAGTGTAATCCCACCAGTTGATGTGGAATCGAGAGTTGTGGAAAAGGAAGAACTTAAACCGAAGGAAGTTTCACATGAGGTCTCCGTCAGTGTTTTAAGTGATAGCAGTAACTTGGTGAATGGTGATAGCGTCTCCAAACAAAATGGCGAATCATCAACAGGCAGCGGCCTACATTTAACGTCAGAGAATCTTAGTCCTGAAAATGTTTCTGAGAAAAGGGCAAGTTTCTTTTACATTTCTTTTATTGCATCAAAGCCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

27.24

Weight (kDa)

5.12

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 727
AciI CCGC 1 cut(s) 630
AclWI GGATC 1 cut(s) 144
AcuI CTGAAG 4 cut(s) 108, 113, 191, 240
AfaI GTAC 2 cut(s) 103, 162
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 3 cut(s) 53, 235, 248
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 6 cut(s) 58, 125, 202, 218, 278, 383
AluI AGCT 6 cut(s) 58, 125, 202, 218, 278, 383
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 550, 601
AlwI GGATC 1 cut(s) 144
AoxI GGCC 2 cut(s) 91, 631
ApeKI GCWGC 2 cut(s) 278, 627
Asp700I GAANNNNTTC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 592, 599
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 265, 639
BccI CCATC 1 cut(s) 340
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 550, 601
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 341
BglII AGATCT 1 cut(s) 166
BisI GCNGC 3 cut(s) 279, 628, 631
BlsI GCNGC 3 cut(s) 280, 629, 632
BmsI GCATC 1 cut(s) 722
BsaI GGTCTC 1 cut(s) 550
BsaJI CCNNGG 2 cut(s) 180, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 479
BseDI CCNNGG 2 cut(s) 180, 309
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 1 cut(s) 479
BseXI GCAGC 2 cut(s) 265, 639
BshFI GGCC 2 cut(s) 93, 633
BsiHKAI GWGCWC 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 2 cut(s) 550, 601
BsmBI CGTCTC 1 cut(s) 601
BsnI GGCC 2 cut(s) 93, 633
Bso31I GGTCTC 1 cut(s) 550
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 160
Bsp143I GATC 3 cut(s) 136, 166, 289
BspACI CCGC 1 cut(s) 630
BspANI GGCC 2 cut(s) 93, 633
BspCNI CTCAG 1 cut(s) 667
BspHI TCATGA 1 cut(s) 385
BspPI GGATC 1 cut(s) 144
BspQI GCTCTTC 1 cut(s) 132
BspTNI GGTCTC 1 cut(s) 550
BsrGI TGTACA 1 cut(s) 160
BsrI ACTGG 1 cut(s) 479
BssECI CCNNGG 2 cut(s) 180, 309
BssMI GATC 3 cut(s) 136, 166, 289
BssT1I CCWWGG 2 cut(s) 180, 309
Bst6I CTCTTC 1 cut(s) 132
BstAUI TGTACA 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 656, 675
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 3 cut(s) 139, 169, 292
BstMAI GTCTC 2 cut(s) 550, 601
BstMBI GATC 3 cut(s) 136, 166, 289
BstSFI CTRYAG 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 265, 639
BstX2I RGATCY 2 cut(s) 136, 166
BstYI RGATCY 2 cut(s) 136, 166
BsuRI GGCC 2 cut(s) 93, 633
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 559
CciI TCATGA 1 cut(s) 385
CseI GACGC 1 cut(s) 583
Csp6I GTAC 2 cut(s) 102, 161
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 2 cut(s) 386, 539
CviQI GTAC 2 cut(s) 102, 161
DdeI CTNAG 2 cut(s) 656, 675
DpnI GATC 3 cut(s) 138, 168, 291
DpnII GATC 3 cut(s) 136, 166, 289
DraI TTTAAA 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 180, 309
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 550
Eco32I GATATC 1 cut(s) 254
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 4 cut(s) 108, 113, 191, 240
EcoICRI GAGCTC 1 cut(s) 202
EcoRV GATATC 1 cut(s) 254
EcoT14I CCWWGG 2 cut(s) 180, 309
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 2 cut(s) 180, 309
Esp3I CGTCTC 1 cut(s) 601
FaeI CATG 2 cut(s) 389, 542
FaiI YATR 5 cut(s) 46, 272, 387, 540, 727
FalI AAGNNNNNCTT 6 cut(s) 47, 79, 141, 173, 164, 196
FatI CATG 2 cut(s) 385, 538
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 3 cut(s) 279, 628, 631
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 279, 628, 631
FspBI CTAG 1 cut(s) 353
GluI GCNGC 3 cut(s) 279, 628, 631
HaeIII GGCC 2 cut(s) 93, 633
HgaI GACGC 1 cut(s) 583
Hin1II CATG 2 cut(s) 389, 542
HinfI GANTC 7 cut(s) 17, 40, 398, 419, 491, 614, 652
HphI GGTGA 2 cut(s) 592, 599
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 5 cut(s) 132, 171, 397, 649, 676
Hpy188III TCNNGA 5 cut(s) 50, 261, 386, 495, 662
Hpy8I GTNNAC 1 cut(s) 104
HpyAV CCTTC 4 cut(s) 83, 127, 242, 520
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 5 cut(s) 213, 230, 241, 427, 713
HpyF3I CTNAG 2 cut(s) 656, 675
HpySE526I ACGT 1 cut(s) 644
Hsp92II CATG 2 cut(s) 389, 542
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 3 cut(s) 136, 166, 289
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 2 cut(s) 9, 199
LpnPI CCDG 5 cut(s) 203, 246, 492, 609, 675
Lsp1109I GCAGC 2 cut(s) 265, 639
LweI GCATC 1 cut(s) 722
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 2 cut(s) 223, 572
MalI GATC 3 cut(s) 138, 168, 291
MboI GATC 3 cut(s) 136, 166, 289
MboII GAAGA 6 cut(s) 119, 184, 338, 446, 470, 524
MflI RGATCY 2 cut(s) 136, 166
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 2 cut(s) 330, 406
MlyI GAGTC 2 cut(s) 49, 428
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 2 cut(s) 312, 535
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 7 cut(s) 69, 141, 333, 363, 519, 560, 641
MspA1I CMGCKG 1 cut(s) 630
NdeII GATC 3 cut(s) 136, 166, 289
NlaIII CATG 2 cut(s) 389, 542
PagI TCATGA 1 cut(s) 385
PciSI GCTCTTC 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 5 cut(s) 17, 398, 491, 614, 652
PkrI GCNGC 3 cut(s) 280, 629, 632
PleI GAGTC 2 cut(s) 48, 427
PpsI GAGTC 2 cut(s) 48, 427
PsiI TTATAA 1 cut(s) 727
Psp124BI GAGCTC 1 cut(s) 204
PsuI RGATCY 2 cut(s) 136, 166
RsaI GTAC 2 cut(s) 103, 162
RsaNI GTAC 2 cut(s) 102, 161
SacI GAGCTC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 7 cut(s) 69, 141, 333, 363, 519, 560, 641
SatI GCNGC 3 cut(s) 279, 628, 631
Sau3AI GATC 3 cut(s) 136, 166, 289
SchI GAGTC 2 cut(s) 49, 428
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 1 cut(s) 722
SfcI CTRYAG 1 cut(s) 341
Sse9I AATT 2 cut(s) 330, 406
SsiI CCGC 1 cut(s) 630
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 1 cut(s) 204
StyI CCWWGG 2 cut(s) 180, 309
TaiI ACGT 1 cut(s) 647
TaqI TCGA 2 cut(s) 26, 494
TasI AATT 2 cut(s) 330, 406
TatI WGTACW 2 cut(s) 101, 160
TauI GCSGC 1 cut(s) 633
TfiI GAWTC 5 cut(s) 17, 398, 491, 614, 652
Tru1I TTAA 7 cut(s) 69, 141, 333, 363, 519, 560, 641
Tru9I TTAA 7 cut(s) 69, 141, 333, 363, 519, 560, 641
TscAI CASTG 1 cut(s) 559
TseI GCWGC 2 cut(s) 278, 627
TspDTI ATGAA 1 cut(s) 471
TspGWI ACGGA 2 cut(s) 280, 538
TspRI CASTG 1 cut(s) 559
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.