pycom09g18610

Lipase 3 N-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Forward (+)
19396008 .. 19399284
3277 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGTATCATATTGTAGAGCGCAAATTTTGCAGATGTGGGAGGTTTCCTCCAGAGGTCCGAACTGCCATTCCAGTTGATGGGAGATTTGAACATATTGTCTTGTCATGTAATGCCACATCTGATCATGGAATTATCTGGATAGAAAGGGAAGCAGAGAAGGCTTTATTAGAAGAAAGTAGTTCTGAGACCGTAACAACTGCTCCAAAAATACAAAAACTTGAGAGGTTGCAGACCATTGAAAAAGAACACAAAGATGCATTGGAAAGAGCTGTCAGTTTGAATGTGCCTCATGCTGTAACATCCAACGAGGAAGCTCCTGAAGACCAAAAACCAGAGGAGGCTCCAGAGATCGAAAATCAGAGCGAAGACGCTGCAGATACAAAGTTGAAGTCCACTGGTGGAAGGGCAAGGTGGGATGATTTGGTTGAAAAGCTTTTCAAGAAAGATTCTGGGGATGTACTGCTAACACGAGATTCAAATCCCCCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase3_N Lipase_3

Protein Analysis

164

Amino Acids

18.42

Weight (kDa)

5.0

Isoelectric Point (pI)

58.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 77
AcsI RAATTY 1 cut(s) 23
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 1 cut(s) 459
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 7 cut(s) 89, 239, 280, 388, 428, 439, 477
AloI GAACNNNNNNTCC 2 cut(s) 52, 84
AluBI AGCT 3 cut(s) 269, 314, 433
AluI AGCT 3 cut(s) 269, 314, 433
Alw26I GTCTC 1 cut(s) 179
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 1 cut(s) 23
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 55
AvaII GGWCC 1 cut(s) 55
BauI CACGAG 1 cut(s) 468
BbsI GAAGAC 2 cut(s) 327, 372
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 71
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BclI TGATCA 1 cut(s) 121
BcoDI GTCTC 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 342
BmsI GCATC 1 cut(s) 244
BpiI GAAGAC 2 cut(s) 327, 372
BplI GAGNNNNNCTC 2 cut(s) 31, 63
BpmI CTGGAG 2 cut(s) 33, 327
BpuEI CTTGAG 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 477
BsaI GGTCTC 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 71, 400
Bse8I GATNNNNATC 1 cut(s) 477
BseGI GGATG 3 cut(s) 299, 421, 460
BseJI GATNNNNATC 1 cut(s) 477
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 71, 400
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 179
Bso31I GGTCTC 1 cut(s) 179
Bsp143I GATC 2 cut(s) 121, 348
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 1 cut(s) 342
BspMAI CTGCAG 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 179
BsrI ACTGG 2 cut(s) 71, 400
BssMI GATC 2 cut(s) 121, 348
BssSI CACGAG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 27
BstDEI CTNAG 1 cut(s) 183
BstF5I GGATG 3 cut(s) 299, 421, 460
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 2 cut(s) 124, 351
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 2 cut(s) 121, 348
BstMWI GCNNNNNNNGC 2 cut(s) 27, 158
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 2 cut(s) 327, 372
BtsCI GGATG 3 cut(s) 299, 421, 460
BtsIMutI CAGTG 1 cut(s) 393
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 55
CseI GACGC 1 cut(s) 377
Csp6I GTAC 1 cut(s) 458
CviAII CATG 3 cut(s) 105, 125, 290
CviJI RGCY 5 cut(s) 161, 269, 314, 341, 433
CviKI_1 RGCY 5 cut(s) 161, 269, 314, 341, 433
CviQI GTAC 1 cut(s) 458
DdeI CTNAG 1 cut(s) 183
DpnI GATC 2 cut(s) 123, 350
DpnII GATC 2 cut(s) 121, 348
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 259
FaeI CATG 3 cut(s) 108, 128, 293
FaiI YATR 5 cut(s) 9, 93, 106, 126, 291
FatI CATG 3 cut(s) 104, 124, 289
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 3 cut(s) 286, 428, 467
Fsp4HI GCNGC 1 cut(s) 372
GlaI GCGC 1 cut(s) 20
GluI GCNGC 1 cut(s) 372
GsuI CTGGAG 2 cut(s) 33, 327
HgaI GACGC 1 cut(s) 377
HhaI GCGC 1 cut(s) 21
Hin1II CATG 3 cut(s) 108, 128, 293
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 2 cut(s) 446, 473
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 4 cut(s) 59, 121, 184, 360
Hpy188III TCNNGA 5 cut(s) 50, 136, 317, 344, 439
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 2 cut(s) 151, 396
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4V TGCA 4 cut(s) 30, 229, 257, 374
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 158
HpyF3I CTNAG 1 cut(s) 183
Hsp92II CATG 3 cut(s) 108, 128, 293
HspAI GCGC 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 2 cut(s) 121, 348
LmnI GCTCC 3 cut(s) 205, 319, 346
LpnPI CCDG 8 cut(s) 63, 84, 121, 330, 345, 357, 381, 435
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 244
MaeIII GTNAC 2 cut(s) 190, 295
MalI GATC 2 cut(s) 123, 350
MboI GATC 2 cut(s) 121, 348
MboII GAAGA 3 cut(s) 182, 332, 377
MluCI AATT 2 cut(s) 23, 129
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 8 cut(s) 33, 46, 57, 216, 297, 301, 328, 331
Mph1103I ATGCAT 1 cut(s) 259
MslI CAYNNNNRTG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 27, 158
NdeII GATC 2 cut(s) 121, 348
NlaIII CATG 3 cut(s) 108, 128, 293
NlaIV GGNNCC 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 259
PfeI GAWTC 2 cut(s) 446, 473
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 55
PstI CTGCAG 1 cut(s) 376
RsaI GTAC 1 cut(s) 459
RsaNI GTAC 1 cut(s) 458
RseI CAYNNNNRTG 1 cut(s) 252
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 2 cut(s) 121, 348
Sau96I GGNCC 1 cut(s) 55
SetI ASST 7 cut(s) 44, 57, 227, 271, 316, 413, 435
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 2 cut(s) 23, 129
TaaI ACNGT 1 cut(s) 190
TaqI TCGA 1 cut(s) 351
TasI AATT 2 cut(s) 23, 129
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 2 cut(s) 446, 473
TscAI CASTG 1 cut(s) 400
TseI GCWGC 1 cut(s) 371
TspRI CASTG 1 cut(s) 400
Van91I CCANNNNNTGG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.