pycom09g19670

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
N/A
Physical Location & Seq
Forward (+)
20531361 .. 20532598
1238 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 297 bp
ATGTGTATACATCAAAGCCAAGCCAGTGAAACCAGCGAAGCCAAGCCAGTGAGCCGAGTACCGGTTAGGGGCATAAGAGGCACTTGGGACATTTGTAGTCGATTGGGCTTCTTCCGCTTTCTCTGCATAAGCGGCTATTTCGAAAAGCACGGCCTCGTCCTTGTTCCCTCCAACTTCCTCCACTTCCAGATCGATCGCGCTTCAGAGAGAATGGAGACTTTCCTATTCACATCTGAATCCGTGAACGAGGGTCACCCCGACAAGCTGTGTGACCAGATCGATCTGATGCAGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 ko01230 map00270 map01100 map01110 map01230
Pfam Domains
Protein Families
S-AdoMet_synt_C S-AdoMet_synt_M S-AdoMet_synt_N

Protein Analysis

99

Amino Acids

11.23

Weight (kDa)

6.04

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 74 - 93 2e-08 S-adenosylmethionine synthetase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 7
AccII CGCG 1 cut(s) 198
AciI CCGC 2 cut(s) 115, 132
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 2 cut(s) 61, 68
AgeI ACCGGT 1 cut(s) 61
AluBI AGCT 1 cut(s) 265
AluI AGCT 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 209
AoxI GGCC 1 cut(s) 151
AsiGI ACCGGT 1 cut(s) 61
AspLEI GCGC 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 245
AsuII TTCGAA 1 cut(s) 141
BceAI ACGGC 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 1 cut(s) 295
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
BmsI GCATC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 141
Bsa29I ATCGAT 2 cut(s) 192, 279
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 68
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 24, 47
BseCI ATCGAT 2 cut(s) 192, 279
BseLI CCNNNNNNNGG 2 cut(s) 61, 68
BseNI ACTGG 2 cut(s) 24, 47
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 1 cut(s) 153
BshTI ACCGGT 1 cut(s) 61
BshVI ATCGAT 2 cut(s) 192, 279
BsiEI CGRYCG 1 cut(s) 196
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 61, 68
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 189, 193, 276, 280
BspACI CCGC 2 cut(s) 115, 132
BspANI GGCC 1 cut(s) 153
BspDI ATCGAT 2 cut(s) 192, 279
BspFNI CGCG 1 cut(s) 198
BspT104I TTCGAA 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 2 cut(s) 24, 47
BssAI RCCGGY 1 cut(s) 61
BssMI GATC 4 cut(s) 189, 193, 276, 280
BssNAI GTATAC 1 cut(s) 8
Bst1107I GTATAC 1 cut(s) 8
BstBI TTCGAA 1 cut(s) 141
BstEII GGTNACC 1 cut(s) 251
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 4 cut(s) 192, 196, 279, 283
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 4 cut(s) 189, 193, 276, 280
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 4 cut(s) 78, 114, 123, 132
BstPI GGTNACC 1 cut(s) 251
BstUI CGCG 1 cut(s) 198
BstZ17I GTATAC 1 cut(s) 8
Bsu15I ATCGAT 2 cut(s) 192, 279
BsuRI GGCC 1 cut(s) 153
BsuTUI ATCGAT 2 cut(s) 192, 279
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 3 cut(s) 31, 54, 296
CfoI GCGC 1 cut(s) 200
Cfr10I RCCGGY 1 cut(s) 61
ClaI ATCGAT 2 cut(s) 192, 279
Csp6I GTAC 1 cut(s) 59
CspAI ACCGGT 1 cut(s) 61
CviJI RGCY 9 cut(s) 18, 23, 41, 46, 54, 108, 135, 153, 265
CviKI_1 RGCY 9 cut(s) 18, 23, 41, 46, 54, 108, 135, 153, 265
CviQI GTAC 1 cut(s) 59
DpnI GATC 4 cut(s) 191, 195, 278, 282
DpnII GATC 4 cut(s) 189, 193, 276, 280
Eco57I CTGAAG 1 cut(s) 186
Eco91I GGTNACC 1 cut(s) 251
EcoO65I GGTNACC 1 cut(s) 251
FaiI YATR 3 cut(s) 8, 74, 128
FalI AAGNNNNNCTT 2 cut(s) 67, 99
FaqI GGGAC 1 cut(s) 101
FblI GTMKAC 1 cut(s) 7
Fnu4HI GCNGC 1 cut(s) 133
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 1 cut(s) 295
GlaI GCGC 1 cut(s) 199
GluI GCNGC 1 cut(s) 133
HaeIII GGCC 1 cut(s) 153
HapII CCGG 1 cut(s) 62
HhaI GCGC 1 cut(s) 200
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 1 cut(s) 236
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 2 cut(s) 8, 244
Hpy188I TCNGA 3 cut(s) 205, 235, 285
Hpy188III TCNNGA 1 cut(s) 187
Hpy8I GTNNAC 2 cut(s) 8, 244
HpyCH4V TGCA 2 cut(s) 126, 289
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 114, 123, 132
HspAI GCGC 1 cut(s) 198
Kzo9I GATC 4 cut(s) 189, 193, 276, 280
LpnPI CCDG 6 cut(s) 37, 46, 60, 75, 200, 287
LweI GCATC 1 cut(s) 276
MaeI CTAG 1 cut(s) 295
MaeIII GTNAC 2 cut(s) 251, 269
MalI GATC 4 cut(s) 191, 195, 278, 282
MboI GATC 4 cut(s) 189, 193, 276, 280
MboII GAAGA 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 5 cut(s) 71, 164, 178, 188, 241
MspI CCGG 1 cut(s) 62
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 4 cut(s) 78, 114, 123, 132
NdeII GATC 4 cut(s) 189, 193, 276, 280
NmeAIII GCCGAG 1 cut(s) 80
NmuCI GTSAC 2 cut(s) 251, 269
NspV TTCGAA 1 cut(s) 141
PfeI GAWTC 1 cut(s) 236
PinAI ACCGGT 1 cut(s) 61
PkrI GCNGC 1 cut(s) 134
Ple19I CGATCG 1 cut(s) 196
PspEI GGTNACC 1 cut(s) 251
PvuI CGATCG 1 cut(s) 196
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 4 cut(s) 189, 193, 276, 280
SetI ASST 1 cut(s) 267
SfaNI GCATC 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 141
SsiI CCGC 2 cut(s) 115, 132
SspMI CTAG 1 cut(s) 295
TaqI TCGA 4 cut(s) 100, 141, 192, 279
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 236
TscAI CASTG 3 cut(s) 31, 54, 296
TseFI GTSAC 2 cut(s) 251, 269
Tsp45I GTSAC 2 cut(s) 251, 269
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 3 cut(s) 31, 54, 296
XmiI GTMKAC 1 cut(s) 7
XspI CTAG 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.