pycom10g00560

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
598096 .. 602008
3913 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1275 bp
ATGGTAATCATAAGGGCCACCATTGTCCATGATTCCATGGGGACACAGACAGTTAAAAAGAACTGTTGCCTTCCGCTCCCGCGAAAAAGCAGTTTGTTTATTTTATTTTATTTTTTTTCTTTTCCGTGGAACTGGAAAGCCAAGCCTGCAAAAGTCACTCACTGCAATGCCAATATTGATCGAGCTGATCAGCTGAAGCAAGAAATGGTGAAAGAAACTGAGTACTACGATGTCCTCAGCGTCACTCCCTCCGCTTCTGAGGAGGAAATTCGCAAAGCTTATTATCTTAAGCTTATATCTTCATATTCTAGTAAACTTCTATCAGAAATTCAAGTATTTAATAGGGAGAAGCTAATATTTGTGCAGGCAAAGCAAGTTCACCCGGATAAAAATCCGAATGATCCTCAGGCTGCCGAAAGATTTCAGGCAAGCGAAGCTTATCAAGTTTTGAGTGATCCGGTGCAAAGAGATGCGTATGATCGAAATGGAAAGCATTGCATATCTAGGGAAACCATGCTTGATCCCACAGCTGTTTTTGCTTTTCTTTTCGGAAGTGAATTATTTGAGGACTACATAGGACATGTAGCAGTAGCATCAATGGCTTCCTCTCAATTAGCTGGTGAAGGTGACAGCACTGAGAAGGCTGTTCAGAAAGAAAGAGAAGAAAAGCTCGCTGGCTCGCTCAAAGGTCTTCTTAACCAATATGTTCTAGGTGACAAAGCGGGATTCTTAGAGCATGCTGAATCCGAAGCTAAACGACTTTCTGATGCAGCCTTCGGAGTTGATATGCTGCACACTATTGGCTACGTATATTCGAGACAAGCAGCACAAGAGCTTGGAAAGAAAACCATATTCCTAGGGGTGCCATTCGTGGCTGAGTGGGTCCGTAATAAGGGACATTTCTGGAAGTCACAGATGACTGCTGCAAAGGGTGCTTATCAGTTGCTACAGCTTCAGGAAGAAATCCGTCGGCAATTTAAAATGGATGGTAGTGGCCCTGAAAACGATGTTGAATCGCATATACGCTTAAACAAAGACACACTTTTGAACTCATTGTGGAAACTCAATGTGGTGGACATCGAAGTAACGCTCTTGCATGTTTGCCAAATGGTTTTACGGGAAAACAATGTTAGGAAGGAGGAGCTTAAAGCTCGTGCTCTGGCGTTAAAACATCTGGGAAGGATATTTCAGGGTATTGGCAGACTCTTCCGATGCCTTTGTAATCCAGCATACGACGTTGACGACGATGAAGAGATTGTGTACAAAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

425

Amino Acids

48.33

Weight (kDa)

7.61

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 122 - 160 5.2e-11 DnaJ domain
DnaJ-X PF14308 217 - 401 2.8e-50 X-domain of DnaJ-containing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 862
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 1 cut(s) 82
AciI CCGC 4 cut(s) 74, 80, 252, 722
AclWI GGATC 3 cut(s) 395, 449, 515
AcsI RAATTY 2 cut(s) 267, 327
AcuI CTGAAG 2 cut(s) 215, 938
AfaI GTAC 2 cut(s) 224, 1262
AfiI CCNNNNNNNGG 1 cut(s) 892
AflII CTTAAG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 3 cut(s) 332, 1013, 1048
Alw21I GWGCWC 1 cut(s) 1159
Alw26I GTCTC 1 cut(s) 811
AlwI GGATC 3 cut(s) 395, 449, 515
AoxI GGCC 2 cut(s) 15, 994
ApeKI GCWGC 5 cut(s) 410, 770, 790, 824, 923
ApoI RAATTY 2 cut(s) 267, 327
Asp700I GAANNNNTTC 1 cut(s) 420
AspA2I CCTAGG 1 cut(s) 856
AspS9I GGNCC 3 cut(s) 15, 883, 995
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 5 cut(s) 220, 371, 632, 638, 725
AvaII GGWCC 1 cut(s) 883
AvrII CCTAGG 1 cut(s) 856
AxyI CCTNAGG 1 cut(s) 405
BanI GGYRCC 1 cut(s) 862
BauI CACGAG 1 cut(s) 1152
BbsI GAAGAC 1 cut(s) 683
Bbv12I GWGCWC 1 cut(s) 1159
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 5 cut(s) 397, 777, 782, 836, 910
BccI CCATC 1 cut(s) 980
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 1 cut(s) 811
BfaI CTAG 4 cut(s) 309, 504, 710, 857
BfmI CTRYAG 1 cut(s) 947
BfrI CTTAAG 1 cut(s) 287
BisI GCNGC 5 cut(s) 411, 771, 791, 825, 924
BlnI CCTAGG 1 cut(s) 856
BlsI GCNGC 5 cut(s) 412, 772, 792, 826, 925
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 1 cut(s) 383
Bme18I GGWCC 1 cut(s) 883
BmgT120I GGNCC 3 cut(s) 15, 883, 995
BmiI GGNNCC 2 cut(s) 864, 884
BmrFI CCNGG 1 cut(s) 383
BmsI GCATC 4 cut(s) 460, 602, 757, 1202
BpiI GAAGAC 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 383
BsaAI YACGTR 1 cut(s) 808
BsaBI GATNNNNATC 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 36, 125, 856
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse1I ACTGG 1 cut(s) 137
Bse21I CCTNAGG 1 cut(s) 405
Bse3DI GCAATG 2 cut(s) 172, 493
Bse8I GATNNNNATC 1 cut(s) 390
BseDI CCNNGG 3 cut(s) 36, 125, 856
BseGI GGATG 1 cut(s) 991
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 892
BseMI GCAATG 2 cut(s) 172, 493
BseMII CTCAG 6 cut(s) 210, 249, 250, 419, 627, 867
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 2 cut(s) 275, 1154
BseXI GCAGC 5 cut(s) 397, 777, 782, 836, 910
BsgI GTGCAG 2 cut(s) 383, 776
Bsh1236I CGCG 1 cut(s) 82
BshFI GGCC 2 cut(s) 17, 996
BshNI GGYRCC 1 cut(s) 862
BsiHKAI GWGCWC 1 cut(s) 1159
BsiSI CCGG 2 cut(s) 383, 458
BslFI GGGAC 2 cut(s) 55, 909
BslI CCNNNNNNNGG 1 cut(s) 892
BsmAI GTCTC 1 cut(s) 811
BsmFI GGGAC 2 cut(s) 55, 909
BsnI GGCC 2 cut(s) 17, 996
Bsp1286I GDGCHC 1 cut(s) 1159
Bsp1407I TGTACA 1 cut(s) 1260
Bsp143I GATC 6 cut(s) 178, 187, 400, 454, 478, 520
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 4 cut(s) 74, 80, 252, 722
BspANI GGCC 2 cut(s) 17, 996
BspCNI CTCAG 6 cut(s) 211, 249, 250, 418, 628, 868
BspFNI CGCG 1 cut(s) 82
BspLI GGNNCC 2 cut(s) 864, 884
BspPI GGATC 3 cut(s) 395, 449, 515
BspT107I GGYRCC 1 cut(s) 862
BspTI CTTAAG 1 cut(s) 287
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 2 cut(s) 172, 493
BsrGI TGTACA 1 cut(s) 1260
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 3 cut(s) 36, 125, 856
BssMI GATC 6 cut(s) 178, 187, 400, 454, 478, 520
BssSI CACGAG 1 cut(s) 1152
BssT1I CCWWGG 2 cut(s) 36, 856
Bst2BI CACGAG 1 cut(s) 1152
Bst4CI ACNGT 2 cut(s) 52, 65
Bst6I CTCTTC 2 cut(s) 1211, 1245
BstAFI CTTAAG 1 cut(s) 287
BstAPI GCANNNNNTGC 1 cut(s) 932
BstAUI TGTACA 1 cut(s) 1260
BstBAI YACGTR 1 cut(s) 808
BstC8I GCNNGC 7 cut(s) 147, 366, 430, 672, 676, 680, 738
BstDEI CTNAG 7 cut(s) 219, 236, 258, 405, 636, 730, 876
BstDSI CCRYGG 2 cut(s) 36, 125
BstF5I GGATG 1 cut(s) 991
BstFNI CGCG 1 cut(s) 82
BstKTI GATC 6 cut(s) 181, 190, 403, 457, 481, 523
BstMAI GTCTC 1 cut(s) 811
BstMBI GATC 6 cut(s) 178, 187, 400, 454, 478, 520
BstMWI GCNNNNNNNGC 6 cut(s) 146, 370, 434, 536, 599, 932
BstNSI RCATGY 3 cut(s) 584, 740, 1100
BstSCI CCNGG 1 cut(s) 381
BstSFI CTRYAG 1 cut(s) 947
BstSNI TACGTA 1 cut(s) 808
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 5 cut(s) 397, 777, 782, 836, 910
BstV2I GAAGAC 1 cut(s) 683
Bsu36I CCTNAGG 1 cut(s) 405
BsuRI GGCC 2 cut(s) 17, 996
BtgI CCRYGG 2 cut(s) 36, 125
BtsCI GGATG 1 cut(s) 991
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 2 cut(s) 160, 633
Cac8I GCNNGC 7 cut(s) 147, 366, 430, 672, 676, 680, 738
Cfr13I GGNCC 3 cut(s) 15, 883, 995
CseI GACGC 1 cut(s) 229
Csp6I GTAC 2 cut(s) 223, 1261
CviAII CATG 6 cut(s) 29, 37, 514, 581, 737, 1097
CviQI GTAC 2 cut(s) 223, 1261
DdeI CTNAG 7 cut(s) 219, 236, 258, 405, 636, 730, 876
DpnI GATC 6 cut(s) 180, 189, 402, 456, 480, 522
DpnII GATC 6 cut(s) 178, 187, 400, 454, 478, 520
DraI TTTAAA 1 cut(s) 979
Eam1104I CTCTTC 2 cut(s) 1211, 1245
EarI CTCTTC 2 cut(s) 1211, 1245
Eco105I TACGTA 1 cut(s) 808
Eco130I CCWWGG 2 cut(s) 36, 856
Eco47I GGWCC 1 cut(s) 883
Eco57I CTGAAG 2 cut(s) 215, 938
Eco81I CCTNAGG 1 cut(s) 405
EcoT14I CCWWGG 2 cut(s) 36, 856
ErhI CCWWGG 2 cut(s) 36, 856
FaeI CATG 6 cut(s) 32, 40, 517, 584, 740, 1100
FalI AAGNNNNNCTT 6 cut(s) 421, 453, 678, 710, 1026, 1058
FaqI GGGAC 2 cut(s) 55, 909
FatI CATG 6 cut(s) 28, 36, 513, 580, 736, 1096
FauI CCCGC 2 cut(s) 87, 715
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 5 cut(s) 411, 771, 791, 825, 924
FokI GGATG 1 cut(s) 998
Fsp4HI GCNGC 5 cut(s) 411, 771, 791, 825, 924
FspBI CTAG 4 cut(s) 309, 504, 710, 857
GluI GCNGC 5 cut(s) 411, 771, 791, 825, 924
HaeIII GGCC 2 cut(s) 17, 996
HapII CCGG 2 cut(s) 383, 458
HgaI GACGC 1 cut(s) 229
Hin1II CATG 6 cut(s) 32, 40, 517, 584, 740, 1100
HincII GTYRAC 1 cut(s) 1240
HindII GTYRAC 1 cut(s) 1240
HindIII AAGCTT 3 cut(s) 276, 290, 435
HinfI GANTC 5 cut(s) 32, 726, 743, 1013, 1203
HpaII CCGG 2 cut(s) 383, 458
HphI GGTGA 5 cut(s) 220, 371, 632, 638, 725
Hpy166II GTNNAC 5 cut(s) 314, 379, 1075, 1240, 1261
Hpy188I TCNGA 9 cut(s) 259, 325, 396, 551, 651, 748, 766, 779, 1211
Hpy188III TCNNGA 3 cut(s) 816, 904, 956
Hpy8I GTNNAC 5 cut(s) 314, 379, 1075, 1240, 1261
Hpy99I CGWCG 3 cut(s) 972, 1238, 1247
HpyAV CCTTC 6 cut(s) 80, 617, 634, 784, 1129, 1173
HpyCH4III ACNGT 2 cut(s) 52, 65
HpyCH4IV ACGT 2 cut(s) 807, 1236
HpyCH4V TGCA 9 cut(s) 149, 165, 364, 463, 498, 770, 793, 926, 1096
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 370, 434, 536, 599, 932
HpyF3I CTNAG 7 cut(s) 219, 236, 258, 405, 636, 730, 876
HpySE526I ACGT 2 cut(s) 807, 1236
Hsp92II CATG 6 cut(s) 32, 40, 517, 584, 740, 1100
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 6 cut(s) 178, 187, 400, 454, 478, 520
LmnI GCTCC 2 cut(s) 81, 1141
Lsp1109I GCAGC 5 cut(s) 397, 777, 782, 836, 910
LweI GCATC 4 cut(s) 460, 602, 757, 1202
MaeI CTAG 4 cut(s) 309, 504, 710, 857
MaeII ACGT 2 cut(s) 807, 1236
MaeIII GTNAC 6 cut(s) 154, 241, 626, 713, 909, 1084
MalI GATC 6 cut(s) 180, 189, 402, 456, 480, 522
MbiI CCGCTC 1 cut(s) 76
MboI GATC 6 cut(s) 178, 187, 400, 454, 478, 520
MboII GAAGA 6 cut(s) 291, 674, 683, 971, 1198, 1262
MhlI GDGCHC 1 cut(s) 1159
MluCI AATT 5 cut(s) 267, 327, 557, 611, 974
MlyI GAGTC 1 cut(s) 1197
MnlI CCTC 8 cut(s) 245, 253, 256, 259, 414, 559, 616, 1132
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 8 cut(s) 54, 288, 339, 696, 978, 1028, 1146, 1166
MslI CAYNNNNRTG 1 cut(s) 165
MspA1I CMGCKG 2 cut(s) 193, 530
MspCI CTTAAG 1 cut(s) 287
MspI CCGG 2 cut(s) 383, 458
MspR9I CCNGG 1 cut(s) 383
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 6 cut(s) 146, 370, 434, 536, 599, 932
NciI CCSGG 1 cut(s) 383
NcoI CCATGG 1 cut(s) 36
NdeII GATC 6 cut(s) 178, 187, 400, 454, 478, 520
NlaIII CATG 6 cut(s) 32, 40, 517, 584, 740, 1100
NlaIV GGNNCC 2 cut(s) 864, 884
NmuCI GTSAC 5 cut(s) 154, 241, 626, 713, 909
NspI RCATGY 3 cut(s) 584, 740, 1100
PaeI GCATGC 1 cut(s) 740
PciI ACATGT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 2 cut(s) 1239, 1242
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 4 cut(s) 32, 726, 743, 1013
PkrI GCNGC 5 cut(s) 412, 772, 792, 826, 925
PleI GAGTC 1 cut(s) 1197
PpsI GAGTC 1 cut(s) 1197
Ppu21I YACGTR 1 cut(s) 808
PscI ACATGT 1 cut(s) 580
PspN4I GGNNCC 2 cut(s) 864, 884
PspPI GGNCC 3 cut(s) 15, 883, 995
PvuII CAGCTG 2 cut(s) 193, 530
RsaI GTAC 2 cut(s) 224, 1262
RsaNI GTAC 2 cut(s) 223, 1261
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 8 cut(s) 54, 288, 339, 696, 978, 1028, 1146, 1166
SatI GCNGC 5 cut(s) 411, 771, 791, 825, 924
Sau3AI GATC 6 cut(s) 178, 187, 400, 454, 478, 520
Sau96I GGNCC 3 cut(s) 15, 883, 995
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 1 cut(s) 1197
ScrFI CCNGG 1 cut(s) 383
SduI GDGCHC 1 cut(s) 1159
SfaNI GCATC 4 cut(s) 460, 602, 757, 1202
SfcI CTRYAG 1 cut(s) 947
SinI GGWCC 1 cut(s) 883
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SnaBI TACGTA 1 cut(s) 808
SphI GCATGC 1 cut(s) 740
Sse9I AATT 5 cut(s) 267, 327, 557, 611, 974
SsiI CCGC 4 cut(s) 74, 80, 252, 722
SspI AATATT 2 cut(s) 175, 357
SspMI CTAG 4 cut(s) 309, 504, 710, 857
StyD4I CCNGG 1 cut(s) 381
StyI CCWWGG 2 cut(s) 36, 856
TaaI ACNGT 2 cut(s) 52, 65
TaiI ACGT 2 cut(s) 810, 1239
TaqI TCGA 4 cut(s) 181, 481, 815, 1080
TasI AATT 5 cut(s) 267, 327, 557, 611, 974
TatI WGTACW 2 cut(s) 222, 1260
TfiI GAWTC 4 cut(s) 32, 726, 743, 1013
Tru1I TTAA 8 cut(s) 54, 288, 339, 696, 978, 1028, 1146, 1166
Tru9I TTAA 8 cut(s) 54, 288, 339, 696, 978, 1028, 1146, 1166
TscAI CASTG 2 cut(s) 167, 640
TseFI GTSAC 5 cut(s) 154, 241, 626, 713, 909
TseI GCWGC 5 cut(s) 410, 770, 790, 824, 923
Tsp45I GTSAC 5 cut(s) 154, 241, 626, 713, 909
TspDTI ATGAA 2 cut(s) 291, 1263
TspGWI ACGGA 3 cut(s) 114, 875, 956
TspRI CASTG 2 cut(s) 167, 640
Vha464I CTTAAG 1 cut(s) 287
VpaK11BI GGWCC 1 cut(s) 883
XapI RAATTY 2 cut(s) 267, 327
XceI RCATGY 3 cut(s) 584, 740, 1100
XmaJI CCTAGG 1 cut(s) 856
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 4 cut(s) 309, 504, 710, 857
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.