pycom10g01310

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
1361611 .. 1362287
677 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGCTTGTTCTAATTAGTGAAGAGCGTTCACTCGCTCTGAAATGTGACTTGTATAAAAAACAAGAAGAAATTGAGATGTTGCCAGCTTGCAAATTCGAGGTGCCTAACATCCGATCTTGTAGGAATATCTGGAGTAATCTTGAAATTTTTTCACTCTTTAGACTACTGCTACTTGCTACCAAGGCATATCCATCAGCCACCCACTTGCTACTACTTGCTCCAGTGGTCTCACTATCCATCCTTCTCCAGATAGGTATTCATTTATCCTTCCCATCCACCATCTTCTTCTGCTGCACATATCTTAACTTACATTGTCTTTCCAGTTTCGAGTTTCAAAAATTTGGTATATCTGTTGCTCAACTTTTAATTACCAGATATCTCTGCCGCAACCTGGGTCGGCTAATGGCGATGACAGATCCAAGAGCCTCCGGGTCTGGTTCATCTTCACCTCCCCAATGGAAGCACGACGTTTTCTTGAGTTTCAGGGGTGAAGACACCAGCAAGAATTTCATAGGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

174

Amino Acids

19.62

Weight (kDa)

8.72

Isoelectric Point (pI)

60.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 100
AciI CCGC 1 cut(s) 385
AclWI GGATC 1 cut(s) 410
AcsI RAATTY 4 cut(s) 92, 144, 338, 505
AfiI CCNNNNNNNGG 1 cut(s) 391
AgsI TTSAA 2 cut(s) 143, 335
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 232
AlwI GGATC 1 cut(s) 410
AoxI GGCC 1 cut(s) 514
ApeKI GCWGC 1 cut(s) 291
ApoI RAATTY 4 cut(s) 92, 144, 338, 505
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 2 cut(s) 438, 500
BanI GGYRCC 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 498
BbvI GCAGC 1 cut(s) 278
BccI CCATC 4 cut(s) 199, 245, 280, 287
BciT130I CCWGG 1 cut(s) 392
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 232
BisI GCNGC 2 cut(s) 292, 385
BlsI GCNGC 2 cut(s) 293, 386
Bme1390I CCNGG 2 cut(s) 392, 430
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 392, 430
BpiI GAAGAC 1 cut(s) 498
BpmI CTGGAG 3 cut(s) 151, 204, 230
BpuEI CTTGAG 1 cut(s) 496
BpuMI CCSGG 1 cut(s) 430
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 180, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 391
Bse1I ACTGG 2 cut(s) 221, 321
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 2 cut(s) 180, 391
BseGI GGATG 3 cut(s) 108, 237, 272
BseLI CCNNNNNNNGG 1 cut(s) 391
BseNI ACTGG 2 cut(s) 221, 321
BseXI GCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 277
BshFI GGCC 1 cut(s) 516
BshNI GGYRCC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 391
BsmAI GTCTC 1 cut(s) 232
BsnI GGCC 1 cut(s) 516
Bso31I GGTCTC 1 cut(s) 232
Bsp143I GATC 2 cut(s) 113, 415
BspACI CCGC 1 cut(s) 385
BspANI GGCC 1 cut(s) 516
BspLI GGNNCC 1 cut(s) 102
BspPI GGATC 1 cut(s) 410
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 100
BspTNI GGTCTC 1 cut(s) 232
BsrI ACTGG 2 cut(s) 221, 321
BssECI CCNNGG 2 cut(s) 180, 391
BssMI GATC 2 cut(s) 113, 415
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 392
Bst6I CTCTTC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 84, 88
BstF5I GGATG 3 cut(s) 108, 237, 272
BstKTI GATC 2 cut(s) 116, 418
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 2 cut(s) 113, 415
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 390, 428
BstV1I GCAGC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 422
BtsCI GGATG 3 cut(s) 108, 237, 272
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 2 cut(s) 84, 88
CviJI RGCY 5 cut(s) 86, 197, 400, 425, 516
CviKI_1 RGCY 5 cut(s) 86, 197, 400, 425, 516
DpnI GATC 2 cut(s) 115, 417
DpnII GATC 2 cut(s) 113, 415
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 180
Eco31I GGTCTC 1 cut(s) 232
Eco32I GATATC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 390
EcoRV GATATC 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaiI YATR 5 cut(s) 54, 187, 298, 347, 512
Fnu4HI GCNGC 2 cut(s) 292, 385
FokI GGATG 3 cut(s) 95, 224, 259
Fsp4HI GCNGC 2 cut(s) 292, 385
GluI GCNGC 2 cut(s) 292, 385
GsuI CTGGAG 3 cut(s) 151, 204, 230
HaeIII GGCC 1 cut(s) 516
HapII CCGG 1 cut(s) 429
HpaII CCGG 1 cut(s) 429
HphI GGTGA 2 cut(s) 438, 500
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 2 cut(s) 39, 113
Hpy188III TCNNGA 4 cut(s) 130, 140, 247, 475
Hpy8I GTNNAC 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 251, 277
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 2 cut(s) 90, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpySE526I ACGT 1 cut(s) 468
Kzo9I GATC 2 cut(s) 113, 415
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 278
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 1 cut(s) 44
MalI GATC 2 cut(s) 115, 417
MboI GATC 2 cut(s) 113, 415
MboII GAAGA 6 cut(s) 32, 77, 274, 277, 435, 503
MflI RGATCY 1 cut(s) 415
MluCI AATT 7 cut(s) 12, 69, 92, 144, 338, 366, 505
MnlI CCTC 3 cut(s) 91, 436, 459
MseI TTAA 2 cut(s) 303, 365
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 2 cut(s) 392, 430
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 1 cut(s) 430
NdeII GATC 2 cut(s) 113, 415
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 44
PciSI GCTCTTC 1 cut(s) 15
PkrI GCNGC 2 cut(s) 293, 386
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 102
PsuI RGATCY 1 cut(s) 415
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 303, 365
SatI GCNGC 2 cut(s) 292, 385
Sau3AI GATC 2 cut(s) 113, 415
ScrFI CCNGG 2 cut(s) 392, 430
SetI ASST 6 cut(s) 88, 102, 256, 393, 451, 471
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 7 cut(s) 12, 69, 92, 144, 338, 366, 505
SsiI CCGC 1 cut(s) 385
StyD4I CCNGG 2 cut(s) 390, 428
StyI CCWWGG 1 cut(s) 180
TaiI ACGT 1 cut(s) 471
TaqI TCGA 2 cut(s) 96, 327
TasI AATT 7 cut(s) 12, 69, 92, 144, 338, 366, 505
TauI GCSGC 1 cut(s) 387
Tru1I TTAA 2 cut(s) 303, 365
Tru9I TTAA 2 cut(s) 303, 365
TscAI CASTG 1 cut(s) 228
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 1 cut(s) 291
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 3 cut(s) 248, 429, 499
TspRI CASTG 1 cut(s) 228
XapI RAATTY 4 cut(s) 92, 144, 338, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.