pycom10g01320

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
1362877 .. 1363425
549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGACACATTGCAACTCAGAGCGATTAAAAACTTTCAGAGACGACCTAGAACTTGAAAGAGGCAAAAATGTTTCTCCAGATCTCGTGTGCAATAGAAAAATCAAGGTTGGGCATCGCTCTCCTCGAATTATGATTCCTGCGCCTGGTGCTTGGATGAACTTTCAAAGATTCTTGAATGCATGGAAGCTAGGAATGCAAGCTTGCCAATATTTTATGATGTGGATCCCTACAAATGTTCGAAACCAAATGGGGGACTTTTGTGAATTCTACAACAAGCACGAAAGAAACTTCAGAGAAGACTTAGCAAAGGCGCAACATTGGAGAGCTGCTTTATCAAAAGTGGGTAATATCGCTCGGTGTACTTCAGAGGATAGGCAAGCATCACCAAGGAGCAGCATCATCCAAGATGGGATGTTTGCAAGGATTTTCTCCTTAATCTTCACCCACAGGGCCAACAAAAGTACTGTCCATTTCATGTGTGCATTGGGACTCTTAGGTGTTGTCTTTGTGGCAATTGCAGCCAAGAGATGCTGCATATCTTTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM NB-ARC TIR

Protein Analysis

183

Amino Acids

21.02

Weight (kDa)

9.84

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 217, 230
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 2 cut(s) 274, 348
AfaI GTAC 2 cut(s) 361, 463
AfiI CCNNNNNNNGG 2 cut(s) 143, 250
AgsI TTSAA 3 cut(s) 56, 164, 175
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 3 cut(s) 187, 200, 326
AluI AGCT 3 cut(s) 187, 200, 326
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 2 cut(s) 217, 230
AoxI GGCC 1 cut(s) 450
ApeKI GCWGC 4 cut(s) 326, 393, 518, 531
ApoI RAATTY 1 cut(s) 263
ArsI GACNNNNNNTTYG 2 cut(s) 450, 482
AspLEI GCGC 2 cut(s) 142, 313
AspS9I GGNCC 1 cut(s) 450
AsuHPI GGTGA 2 cut(s) 375, 433
AsuII TTCGAA 1 cut(s) 238
BamHI GGATCC 1 cut(s) 222
BauI CACGAG 1 cut(s) 83
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 4 cut(s) 313, 405, 518, 530
BccI CCATC 1 cut(s) 401
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 33
BfaI CTAG 2 cut(s) 47, 188
BglII AGATCT 1 cut(s) 79
BisI GCNGC 4 cut(s) 327, 394, 519, 532
BlsI GCNGC 4 cut(s) 328, 395, 520, 533
BmcAI AGTACT 1 cut(s) 463
Bme1390I CCNGG 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 4 cut(s) 121, 389, 405, 518
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 60
Bpu14I TTCGAA 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 386
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 250
Bse3DI GCAATG 1 cut(s) 7
Bse8I GATNNNNATC 1 cut(s) 221
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 3 cut(s) 159, 399, 417
BseJI GATNNNNATC 1 cut(s) 221
BseLI CCNNNNNNNGG 2 cut(s) 143, 250
BseMI GCAATG 1 cut(s) 7
BseMII CTCAG 1 cut(s) 30
BseRI GAGGAG 1 cut(s) 111
BseXI GCAGC 4 cut(s) 313, 405, 518, 530
BshFI GGCC 1 cut(s) 452
BslFI GGGAC 2 cut(s) 266, 501
BslI CCNNNNNNNGG 2 cut(s) 143, 250
BsmAI GTCTC 1 cut(s) 33
BsmBI CGTCTC 1 cut(s) 33
BsmFI GGGAC 2 cut(s) 266, 501
BsmI GAATGC 2 cut(s) 181, 198
BsnI GGCC 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 238
Bsp143I GATC 2 cut(s) 79, 222
BspANI GGCC 1 cut(s) 452
BspCNI CTCAG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 2 cut(s) 217, 230
BspT104I TTCGAA 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 2 cut(s) 79, 222
BssSI CACGAG 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 386
Bst2BI CACGAG 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 466
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 198, 202, 378
BstDEI CTNAG 3 cut(s) 16, 301, 493
BstF5I GGATG 3 cut(s) 159, 399, 417
BstHHI GCGC 2 cut(s) 142, 313
BstKTI GATC 2 cut(s) 82, 225
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 2 cut(s) 79, 222
BstMWI GCNNNNNNNGC 3 cut(s) 146, 193, 518
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstV1I GCAGC 4 cut(s) 313, 405, 518, 530
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 2 cut(s) 79, 222
BstYI RGATCY 2 cut(s) 79, 222
BsuRI GGCC 1 cut(s) 452
BtgZI GCGATG 1 cut(s) 98
BtsCI GGATG 3 cut(s) 159, 399, 417
Cac8I GCNNGC 3 cut(s) 198, 202, 378
CfoI GCGC 2 cut(s) 142, 313
Cfr13I GGNCC 1 cut(s) 450
Csp6I GTAC 2 cut(s) 360, 462
CviAII CATG 2 cut(s) 180, 475
CviJI RGCY 5 cut(s) 187, 200, 326, 452, 521
CviKI_1 RGCY 5 cut(s) 187, 200, 326, 452, 521
CviQI GTAC 2 cut(s) 360, 462
DdeI CTNAG 3 cut(s) 16, 301, 493
DpnI GATC 2 cut(s) 81, 224
DpnII GATC 2 cut(s) 79, 222
Eco130I CCWWGG 1 cut(s) 386
Eco57I CTGAAG 2 cut(s) 274, 348
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 386
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 2 cut(s) 183, 478
FaiI YATR 5 cut(s) 131, 181, 215, 476, 536
FaqI GGGAC 2 cut(s) 266, 501
FatI CATG 2 cut(s) 179, 474
Fnu4HI GCNGC 4 cut(s) 327, 394, 519, 532
FokI GGATG 3 cut(s) 166, 386, 424
Fsp4HI GCNGC 4 cut(s) 327, 394, 519, 532
FspBI CTAG 2 cut(s) 47, 188
GlaI GCGC 2 cut(s) 141, 312
GluI GCNGC 4 cut(s) 327, 394, 519, 532
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 1 cut(s) 452
HhaI GCGC 2 cut(s) 142, 313
Hin1II CATG 2 cut(s) 183, 478
Hin6I GCGC 2 cut(s) 140, 311
HinP1I GCGC 2 cut(s) 140, 311
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 3 cut(s) 133, 168, 489
HphI GGTGA 2 cut(s) 375, 433
Hpy166II GTNNAC 1 cut(s) 360
Hpy188I TCNGA 4 cut(s) 19, 38, 293, 367
Hpy188III TCNNGA 2 cut(s) 77, 172
Hpy8I GTNNAC 1 cut(s) 360
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 8 cut(s) 12, 90, 179, 196, 419, 482, 518, 534
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 193, 518
HpyF3I CTNAG 3 cut(s) 16, 301, 493
Hsp92II CATG 2 cut(s) 183, 478
HspAI GCGC 2 cut(s) 140, 311
Kzo9I GATC 2 cut(s) 79, 222
LmnI GCTCC 1 cut(s) 390
LpnPI CCDG 5 cut(s) 90, 129, 150, 156, 433
Lsp1109I GCAGC 4 cut(s) 313, 405, 518, 530
LweI GCATC 4 cut(s) 121, 389, 405, 518
MaeI CTAG 2 cut(s) 47, 188
MalI GATC 2 cut(s) 81, 224
MboI GATC 2 cut(s) 79, 222
MboII GAAGA 2 cut(s) 308, 430
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 79, 222
MluCI AATT 3 cut(s) 126, 263, 513
MlyI GAGTC 1 cut(s) 483
MnlI CCTC 3 cut(s) 53, 132, 361
Mph1103I ATGCAT 1 cut(s) 181
MseI TTAA 2 cut(s) 26, 434
MspR9I CCNGG 1 cut(s) 144
MunI CAATTG 1 cut(s) 513
Mva1269I GAATGC 2 cut(s) 181, 198
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 146, 193, 518
NdeII GATC 2 cut(s) 79, 222
NlaIII CATG 2 cut(s) 183, 478
NlaIV GGNNCC 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 181
NspV TTCGAA 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PctI GAATGC 2 cut(s) 181, 198
PfeI GAWTC 2 cut(s) 133, 168
PkrI GCNGC 4 cut(s) 328, 395, 520, 533
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 1 cut(s) 450
PsuI RGATCY 2 cut(s) 79, 222
RsaI GTAC 2 cut(s) 361, 463
RsaNI GTAC 2 cut(s) 360, 462
SaqAI TTAA 2 cut(s) 26, 434
SatI GCNGC 4 cut(s) 327, 394, 519, 532
Sau3AI GATC 2 cut(s) 79, 222
Sau96I GGNCC 1 cut(s) 450
ScaI AGTACT 1 cut(s) 463
SchI GAGTC 1 cut(s) 483
ScrFI CCNGG 1 cut(s) 144
SetI ASST 6 cut(s) 48, 108, 189, 202, 328, 499
SfaNI GCATC 4 cut(s) 121, 389, 405, 518
SfuI TTCGAA 1 cut(s) 238
Sse9I AATT 3 cut(s) 126, 263, 513
SspI AATATT 1 cut(s) 209
SspMI CTAG 2 cut(s) 47, 188
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 2 cut(s) 124, 238
TasI AATT 3 cut(s) 126, 263, 513
TatI WGTACW 2 cut(s) 359, 461
TfiI GAWTC 2 cut(s) 133, 168
Tru1I TTAA 2 cut(s) 26, 434
Tru9I TTAA 2 cut(s) 26, 434
TseI GCWGC 4 cut(s) 326, 393, 518, 531
TspDTI ATGAA 2 cut(s) 170, 463
XapI RAATTY 1 cut(s) 263
XspI CTAG 2 cut(s) 47, 188
ZrmI AGTACT 1 cut(s) 463
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.