pycom10g03150

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
3502458 .. 3502778
321 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 321 bp
ATGGTGGCATTGGGATGTGCATTTGTGATCGGCATCATCAACGTGACGTGGATAGTGATTTTAAGGGGGAGGTGGGCAGGCCTCTTCACCAAGGACGAGCTCGTCAAAGCCTTGGTTGCATCAGTCATGCCAATCATGGGGCTTCGTGAGTTAGGCAACTGCCCCCAAACCACTGGCTGTGGCATCCTACGTGTCATGGCACGCCCGGCTGTGGGTGCCCGCATTATTTTGGGGTCGTTCTACTTTGTGGGCACCCCTGTGGCAGTCGGGCTTGCCTTCTGGCTCAAGGTAGGGTTTGGCGGGCTCTGGTTCAGGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

107

Amino Acids

11.41

Weight (kDa)

10.43

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 3 - 88 1.3e-09 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 101
AccB1I GGYRCC 2 cut(s) 215, 251
AciI CCGC 2 cut(s) 220, 300
AfiI CCNNNNNNNGG 3 cut(s) 137, 211, 212
AflIII ACRYGT 1 cut(s) 190
AjiI CACGTC 1 cut(s) 48
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 1 cut(s) 100
AluI AGCT 1 cut(s) 100
Alw21I GWGCWC 1 cut(s) 102
AoxI GGCC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 79
BaeGI GKGCMC 2 cut(s) 220, 254
BanI GGYRCC 2 cut(s) 215, 251
BanII GRGCYC 2 cut(s) 102, 306
Bbv12I GWGCWC 1 cut(s) 102
BcnI CCSGG 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 206
BmgBI CACGTC 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 217, 253
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 3 cut(s) 42, 128, 192
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 206
BsaAI YACGTR 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 90, 111
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 211, 212
Bse1I ACTGG 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 90, 111
BseGI GGATG 2 cut(s) 20, 183
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 3 cut(s) 137, 211, 212
BseNI ACTGG 1 cut(s) 178
BseSI GKGCMC 2 cut(s) 220, 254
BshFI GGCC 1 cut(s) 81
BshNI GGYRCC 2 cut(s) 215, 251
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 3 cut(s) 137, 211, 212
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 4 cut(s) 102, 220, 254, 306
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 2 cut(s) 220, 300
BspANI GGCC 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 217, 253
BspT107I GGYRCC 2 cut(s) 215, 251
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 90, 111
BssMI GATC 1 cut(s) 27
BssT1I CCWWGG 2 cut(s) 90, 111
Bst6I CTCTTC 1 cut(s) 89
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 5 cut(s) 79, 202, 220, 273, 302
BstF5I GGATG 2 cut(s) 20, 183
BstKTI GATC 1 cut(s) 30
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 3 cut(s) 116, 206, 215
BstSCI CCNGG 1 cut(s) 204
BstSLI GKGCMC 2 cut(s) 220, 254
BstXI CCANNNNNNTGG 1 cut(s) 173
BsuRI GGCC 1 cut(s) 81
BtrI CACGTC 1 cut(s) 48
BtsCI GGATG 2 cut(s) 20, 183
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 5 cut(s) 79, 202, 220, 273, 302
CviAII CATG 3 cut(s) 127, 136, 196
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 90, 111
Eco147I AGGCCT 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 102, 306
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoT14I CCWWGG 2 cut(s) 90, 111
EcoT38I GRGCYC 2 cut(s) 102, 306
ErhI CCWWGG 2 cut(s) 90, 111
FaeI CATG 3 cut(s) 130, 139, 199
FaiI YATR 3 cut(s) 128, 137, 197
FatI CATG 3 cut(s) 126, 135, 195
FauI CCCGC 2 cut(s) 227, 293
FokI GGATG 2 cut(s) 27, 170
FriOI GRGCYC 2 cut(s) 102, 306
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 206
Hin1II CATG 3 cut(s) 130, 139, 199
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 79
Hpy188III TCNNGA 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 286
HpyCH4IV ACGT 3 cut(s) 42, 47, 190
HpyCH4V TGCA 2 cut(s) 20, 119
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 206, 215
HpySE526I ACGT 3 cut(s) 42, 47, 190
Hsp92II CATG 3 cut(s) 130, 139, 199
Kzo9I GATC 1 cut(s) 27
LpnPI CCDG 7 cut(s) 63, 159, 219, 265, 270, 292, 298
LweI GCATC 3 cut(s) 42, 128, 192
MaeII ACGT 3 cut(s) 42, 47, 190
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 1 cut(s) 76
MhlI GDGCHC 4 cut(s) 102, 220, 254, 306
MnlI CCTC 2 cut(s) 63, 92
MseI TTAA 2 cut(s) 62, 319
MslI CAYNNNNRTG 3 cut(s) 13, 41, 257
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 206
MwoI GCNNNNNNNGC 3 cut(s) 116, 206, 215
NciI CCSGG 1 cut(s) 206
NdeII GATC 1 cut(s) 27
NlaIII CATG 3 cut(s) 130, 139, 199
NlaIV GGNNCC 2 cut(s) 217, 253
NmuCI GTSAC 1 cut(s) 43
OliI CACNNNNGTG 1 cut(s) 257
PceI AGGCCT 1 cut(s) 81
Ppu21I YACGTR 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 217, 253
RseI CAYNNNNRTG 3 cut(s) 13, 41, 257
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 2 cut(s) 62, 319
Sau3AI GATC 1 cut(s) 27
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 4 cut(s) 102, 220, 254, 306
SetI ASST 6 cut(s) 45, 50, 74, 102, 193, 291
SfaNI GCATC 3 cut(s) 42, 128, 192
SmiMI CAYNNNNRTG 3 cut(s) 13, 41, 257
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SseBI AGGCCT 1 cut(s) 81
SsiI CCGC 2 cut(s) 220, 300
SstI GAGCTC 1 cut(s) 102
StuI AGGCCT 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 204
StyI CCWWGG 2 cut(s) 90, 111
TaiI ACGT 3 cut(s) 45, 50, 193
Tru1I TTAA 2 cut(s) 62, 319
Tru9I TTAA 2 cut(s) 62, 319
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 1 cut(s) 43
Tsp45I GTSAC 1 cut(s) 43
TspRI CASTG 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.