pycom10g08160

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
10536879 .. 10539362
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGAATAAGAACACCAGGCCTCAAGATCTTCTGGGGCCACGGCCTCATTGGCCGGCATGGCCCTCCTTGTCCTCCTCCCCCTCGCGGCCGCCATGGACCTGTCCATCATCGACTACAATGACAAGCACGTCCTGGTTCATCAAGCACGGCAAAAATTACAACGCTTTGGGCGAGAAGGAGCGGAGATTCGAGATTTTTAAGGATAATCTGAGGTTCATCGACGAGAGTAACGGTCTGTTTACTTGGGCGCCAAGGTGGACCGGGGTTCCAGGCTTTCTCGGTCGAGGAAGAGCGACCGGTACACTTTCCGGGCTGACGACGAGTTGCCGGAGTCCGTCGATTGGAGAGCTAAGGGCGCTGTTGCTGCGTGAAGGGAGTTGCTGGGCGTTCTCAACAGTCGGCACAGTGGAAGGAATAAACAAGATTGTGACTGGTGAGCTCATCTCTTTGTCTGAGCAAGAACTAGTGGATTGTGACAGATCATACAACCAGGGATGCAATGGAGGTCTTATGGACTATGCCTTCCAATTCATCATCAACAATGGCCGCATTGATGCTGAAGCAGATTACCCTTACCAGGCTCGAGATGGTTTGTGTGATCCTAACAGGGCCTGGGCTGTGTCCATTGATGGGTATGAAGATGTTCCAGAAAATGATGAGAAATCATTGAAGAAGGCTGTGGCACATCAACCAGTTAGTGTTGCCATTGAAGCTGGTGGCAGGGAATTCCAGCTTTACCAATCGGGTGTCTTCACTGGACGTTGTGGAACAGATCTAGATCACGGTGTGGTTGCTGTTGGTTATGGTACCGAAAATGGTGTAGACTACTCGATCGTGAGGAACTCATGGGGTCCCAGCTGGGGCGAGGCTGGTTACATCAGGTTGGAACGGAATGTGGCTAGCGCCAATACTGGCAAATGTGAACCCCCCAGACCTCCCCCATACCCACCATCTCCTCCTCCTGTGAAGCCCCCTACCGTGTGCGATGAGTACTACTCGTGCCCCGTGGGAACTACATGCTGTTGCCCTCTAGAGTCTGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCTCGTGAGTACACCGTTTGCGACCTTGATGCCGGAACTTGCCGATTGAGCAAGGACAACCCATTGAGCGTGAAAGCGCTAAAGCGAACTCCTGCTAGAAGTAGCCGGCGTTTCCGCTTTCCTAGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004842 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005764 GO:0005773 GO:0005829 GO:0005911 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009888 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010494 GO:0010498 GO:0010605 GO:0016020 GO:0016049 GO:0016567 GO:0016740 GO:0016787 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0030163 GO:0031146 GO:0031224 GO:0031225 GO:0031347 GO:0031348 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0035770 GO:0036211 GO:0036464 GO:0040007 GO:0042176 GO:0042177 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048046 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0060255 GO:0060560 GO:0065007 GO:0070011 GO:0070647 GO:0071695 GO:0071704 GO:0080090 GO:0080134 GO:0080147 GO:0090558 GO:0090627 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

401

Amino Acids

44.07

Weight (kDa)

6.54

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 44 - 77 7.1e-08 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 300 7.5e-68 Papain family cysteine protease
Granulin PF00396 337 - 371 2.9e-06 Granulin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
Acc36I ACCTGC 1 cut(s) 1052
Acc65I GGTACC 1 cut(s) 806
AccB1I GGYRCC 2 cut(s) 247, 806
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 1 cut(s) 822
AccII CGCG 1 cut(s) 85
AciI CCGC 5 cut(s) 85, 89, 181, 547, 1186
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 3 cut(s) 50, 86, 544
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 1 cut(s) 579
AcyI GRCGYC 1 cut(s) 248
AdeI CACNNNGTG 1 cut(s) 787
AfaI GTAC 4 cut(s) 301, 808, 992, 1082
AfeI AGCGCT 1 cut(s) 1149
AfiI CCNNNNNNNGG 5 cut(s) 84, 341, 577, 630, 860
AgeI ACCGGT 1 cut(s) 296
AgsI TTSAA 2 cut(s) 670, 710
AhlI ACTAGT 1 cut(s) 463
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 6 cut(s) 14, 131, 268, 489, 576, 611
AluBI AGCT 5 cut(s) 349, 439, 713, 733, 858
AluI AGCT 5 cut(s) 349, 439, 713, 733, 858
Alw21I GWGCWC 1 cut(s) 441
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 2 cut(s) 612, 1068
Ama87I CYCGRG 1 cut(s) 582
Aor51HI AGCGCT 1 cut(s) 1149
AoxI GGCC 8 cut(s) 17, 35, 41, 50, 59, 86, 544, 609
ApeKI GCWGC 2 cut(s) 364, 1068
ApoI RAATTY 1 cut(s) 725
AsiGI ACCGGT 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 642
Asp718I GGTACC 1 cut(s) 806
AspA2I CCTAGG 1 cut(s) 1193
AspLEI GCGC 4 cut(s) 250, 358, 905, 1150
AspS9I GGNCC 6 cut(s) 35, 60, 96, 258, 609, 851
AsuC2I CCSGG 2 cut(s) 262, 310
AsuHPI GGTGA 1 cut(s) 446
AsuNHI GCTAGC 1 cut(s) 899
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 3 cut(s) 96, 258, 851
AvrII CCTAGG 1 cut(s) 1193
BaeGI GKGCMC 1 cut(s) 1004
BanI GGYRCC 2 cut(s) 247, 806
BanII GRGCYC 1 cut(s) 441
BauI CACGAG 2 cut(s) 997, 1074
BbsI GAAGAC 1 cut(s) 742
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 351, 1055
BccI CCATC 4 cut(s) 112, 581, 623, 958
BceAI ACGGC 2 cut(s) 56, 163
BcgI CGANNNNNNTGC 2 cut(s) 1082, 1116
BciT130I CCWGG 6 cut(s) 16, 133, 270, 491, 578, 613
BcnI CCSGG 2 cut(s) 262, 310
BcuI ACTAGT 1 cut(s) 463
BfaI CTAG 6 cut(s) 464, 776, 900, 1031, 1167, 1194
BfoI RGCGCY 4 cut(s) 251, 359, 906, 1151
BfuAI ACCTGC 1 cut(s) 1052
BglI GCCNNNNNGGC 2 cut(s) 49, 58
BglII AGATCT 2 cut(s) 25, 772
BisI GCNGC 5 cut(s) 86, 89, 365, 547, 1069
BlnI CCTAGG 1 cut(s) 1193
BlsI GCNGC 5 cut(s) 87, 90, 366, 548, 1070
BmcAI AGTACT 1 cut(s) 992
Bme1390I CCNGG 8 cut(s) 16, 133, 262, 270, 310, 491, 578, 613
Bme18I GGWCC 3 cut(s) 96, 258, 851
BmeT110I CYCGRG 1 cut(s) 582
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 6 cut(s) 35, 60, 96, 258, 609, 851
BmiI GGNNCC 6 cut(s) 36, 249, 267, 808, 852, 853
BmrFI CCNGG 8 cut(s) 16, 133, 262, 270, 310, 491, 578, 613
BmsI GCATC 3 cut(s) 485, 544, 1090
BmtI GCTAGC 1 cut(s) 903
BpiI GAAGAC 1 cut(s) 742
BplI GAGNNNNNCTC 4 cut(s) 428, 460, 980, 1012
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 6
BpuMI CCSGG 2 cut(s) 262, 310
BsaBI GATNNNNATC 1 cut(s) 777
BsaHI GRCGYC 1 cut(s) 248
BsaJI CCNNGG 8 cut(s) 38, 92, 251, 261, 490, 612, 1005, 1193
BsaWI WCCGGW 1 cut(s) 296
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 341, 577, 630, 860
Bse118I RCCGGY 3 cut(s) 52, 296, 1176
Bse1I ACTGG 4 cut(s) 436, 692, 760, 916
Bse3DI GCAATG 1 cut(s) 505
Bse8I GATNNNNATC 1 cut(s) 777
BseBI CCWGG 6 cut(s) 16, 133, 270, 491, 578, 613
BseDI CCNNGG 8 cut(s) 38, 92, 251, 261, 490, 612, 1005, 1193
BseGI GGATG 1 cut(s) 500
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 5 cut(s) 84, 341, 577, 630, 860
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 2 cut(s) 200, 444
BseNI ACTGG 4 cut(s) 436, 692, 760, 916
BseRI GAGGAG 3 cut(s) 64, 945, 948
BseSI GKGCMC 1 cut(s) 1004
BseX3I CGGCCG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 351, 1055
BseYI CCCAGC 3 cut(s) 381, 854, 858
Bsh1236I CGCG 1 cut(s) 85
Bsh1285I CGRYCG 4 cut(s) 89, 283, 297, 834
BshFI GGCC 8 cut(s) 19, 37, 43, 52, 61, 88, 546, 611
BshNI GGYRCC 2 cut(s) 247, 806
BshTI ACCGGT 1 cut(s) 296
BsiEI CGRYCG 4 cut(s) 89, 283, 297, 834
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 7 cut(s) 53, 261, 297, 309, 328, 1104, 1177
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 5 cut(s) 84, 341, 577, 630, 860
BsmFI GGGAC 1 cut(s) 837
BsnI GGCC 8 cut(s) 19, 37, 43, 52, 61, 88, 546, 611
BsoBI CYCGRG 1 cut(s) 582
Bsp1286I GDGCHC 2 cut(s) 441, 1004
Bsp143I GATC 6 cut(s) 25, 479, 598, 772, 778, 831
Bsp19I CCATGG 1 cut(s) 92
BspACI CCGC 5 cut(s) 85, 89, 181, 547, 1186
BspANI GGCC 8 cut(s) 19, 37, 43, 52, 61, 88, 546, 611
BspCNI CTCAG 2 cut(s) 201, 445
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 6 cut(s) 36, 249, 267, 808, 852, 853
BspMI ACCTGC 1 cut(s) 1052
BspOI GCTAGC 1 cut(s) 903
BspPI GGATC 1 cut(s) 593
BspQI GCTCTTC 1 cut(s) 283
BspT107I GGYRCC 2 cut(s) 247, 806
BsrBI CCGCTC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 505
BsrFI RCCGGY 3 cut(s) 52, 296, 1176
BsrI ACTGG 4 cut(s) 436, 692, 760, 916
BssAI RCCGGY 3 cut(s) 52, 296, 1176
BssECI CCNNGG 8 cut(s) 38, 92, 251, 261, 490, 612, 1005, 1193
BssMI GATC 6 cut(s) 25, 479, 598, 772, 778, 831
BssNI GRCGYC 1 cut(s) 248
BssSI CACGAG 2 cut(s) 997, 1074
BssT1I CCWWGG 3 cut(s) 92, 251, 1193
Bst2BI CACGAG 2 cut(s) 997, 1074
Bst2UI CCWGG 6 cut(s) 16, 133, 270, 491, 578, 613
Bst4CI ACNGT 7 cut(s) 233, 397, 406, 785, 979, 1064, 1087
Bst6I CTCTTC 1 cut(s) 283
BstACI GRCGYC 1 cut(s) 248
BstC8I GCNNGC 3 cut(s) 54, 901, 1178
BstDEI CTNAG 3 cut(s) 209, 350, 453
BstDSI CCRYGG 3 cut(s) 38, 92, 1005
BstF5I GGATG 1 cut(s) 500
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 4 cut(s) 251, 359, 906, 1151
BstHHI GCGC 4 cut(s) 250, 358, 905, 1150
BstKTI GATC 6 cut(s) 28, 482, 601, 775, 781, 834
BstMBI GATC 6 cut(s) 25, 479, 598, 772, 778, 831
BstMCI CGRYCG 4 cut(s) 89, 283, 297, 834
BstMWI GCNNNNNNNGC 6 cut(s) 49, 58, 355, 364, 710, 1119
BstNI CCWGG 6 cut(s) 16, 133, 270, 491, 578, 613
BstNSI RCATGY 1 cut(s) 1020
BstSCI CCNGG 8 cut(s) 14, 131, 260, 268, 308, 489, 576, 611
BstSLI GKGCMC 1 cut(s) 1004
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 351, 1055
BstV2I GAAGAC 1 cut(s) 742
BstX2I RGATCY 2 cut(s) 25, 772
BstYI RGATCY 2 cut(s) 25, 772
BstZI CGGCCG 1 cut(s) 86
BsuRI GGCC 8 cut(s) 19, 37, 43, 52, 61, 88, 546, 611
BtgI CCRYGG 3 cut(s) 38, 92, 1005
BtgZI GCGATG 1 cut(s) 999
BtrI CACGTC 1 cut(s) 129
BtsCI GGATG 1 cut(s) 500
BtsIMutI CAGTG 2 cut(s) 411, 753
BveI ACCTGC 1 cut(s) 1052
Cac8I GCNNGC 3 cut(s) 54, 901, 1178
CaiI CAGNNNCTG 2 cut(s) 612, 1068
CciNI GCGGCCGC 1 cut(s) 86
CfoI GCGC 4 cut(s) 250, 358, 905, 1150
Cfr10I RCCGGY 3 cut(s) 52, 296, 1176
Cfr13I GGNCC 6 cut(s) 35, 60, 96, 258, 609, 851
Csp6I GTAC 4 cut(s) 300, 807, 991, 1081
CspAI ACCGGT 1 cut(s) 296
CviAII CATG 4 cut(s) 57, 93, 846, 1017
CviQI GTAC 4 cut(s) 300, 807, 991, 1081
DdeI CTNAG 3 cut(s) 209, 350, 453
DinI GGCGCC 1 cut(s) 249
DpnI GATC 6 cut(s) 27, 481, 600, 774, 780, 833
DpnII GATC 6 cut(s) 25, 479, 598, 772, 778, 831
DraIII CACNNNGTG 1 cut(s) 787
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 3 cut(s) 50, 86, 544
EagI CGGCCG 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Ecl136II GAGCTC 1 cut(s) 439
EclXI CGGCCG 1 cut(s) 86
Eco130I CCWWGG 3 cut(s) 92, 251, 1193
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 441
Eco47I GGWCC 3 cut(s) 96, 258, 851
Eco47III AGCGCT 1 cut(s) 1149
Eco52I CGGCCG 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 579
Eco88I CYCGRG 1 cut(s) 582
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 2 cut(s) 609, 851
EcoRI GAATTC 1 cut(s) 725
EcoRII CCWGG 6 cut(s) 14, 131, 268, 489, 576, 611
EcoT14I CCWWGG 3 cut(s) 92, 251, 1193
EcoT38I GRGCYC 1 cut(s) 441
EgeI GGCGCC 1 cut(s) 249
EheI GGCGCC 1 cut(s) 249
ErhI CCWWGG 3 cut(s) 92, 251, 1193
FaeI CATG 4 cut(s) 60, 96, 849, 1020
FaqI GGGAC 1 cut(s) 837
FatI CATG 4 cut(s) 56, 92, 845, 1016
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 5 cut(s) 86, 89, 365, 547, 1069
FokI GGATG 1 cut(s) 507
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 5 cut(s) 86, 89, 365, 547, 1069
FspBI CTAG 6 cut(s) 464, 776, 900, 1031, 1167, 1194
GlaI GCGC 4 cut(s) 249, 357, 904, 1149
GluI GCNGC 5 cut(s) 86, 89, 365, 547, 1069
GsaI CCCAGC 3 cut(s) 385, 858, 862
HaeII RGCGCY 4 cut(s) 251, 359, 906, 1151
HaeIII GGCC 8 cut(s) 19, 37, 43, 52, 61, 88, 546, 611
HapII CCGG 7 cut(s) 53, 261, 297, 309, 328, 1104, 1177
HhaI GCGC 4 cut(s) 250, 358, 905, 1150
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 4 cut(s) 60, 96, 849, 1020
Hin6I GCGC 4 cut(s) 248, 356, 903, 1148
HinP1I GCGC 4 cut(s) 248, 356, 903, 1148
HinfI GANTC 3 cut(s) 186, 331, 1034
HpaII CCGG 7 cut(s) 53, 261, 297, 309, 328, 1104, 1177
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 6 cut(s) 240, 258, 302, 823, 923, 1083
Hpy188I TCNGA 2 cut(s) 210, 454
Hpy188III TCNNGA 8 cut(s) 23, 190, 584, 647, 776, 835, 1031, 1076
Hpy8I GTNNAC 6 cut(s) 240, 258, 302, 823, 923, 1083
Hpy99I CGWCG 3 cut(s) 224, 322, 340
HpyAV CCTTC 5 cut(s) 169, 365, 404, 532, 667
HpyCH4III ACNGT 7 cut(s) 233, 397, 406, 785, 979, 1064, 1087
HpyCH4IV ACGT 2 cut(s) 128, 760
HpyCH4V TGCA 2 cut(s) 498, 1040
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 58, 355, 364, 710, 1119
HpyF3I CTNAG 3 cut(s) 209, 350, 453
HpySE526I ACGT 2 cut(s) 128, 760
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 4 cut(s) 60, 96, 849, 1020
HspAI GCGC 4 cut(s) 248, 356, 903, 1148
KasI GGCGCC 1 cut(s) 247
KflI GGGWCCC 1 cut(s) 851
KpnI GGTACC 1 cut(s) 810
KroI GCCGGC 2 cut(s) 52, 1176
KroNI GCCGGC 2 cut(s) 54, 1178
Kzo9I GATC 6 cut(s) 25, 479, 598, 772, 778, 831
LguI GCTCTTC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 2 cut(s) 351, 1055
LweI GCATC 3 cut(s) 485, 544, 1090
MaeI CTAG 6 cut(s) 464, 776, 900, 1031, 1167, 1194
MaeII ACGT 2 cut(s) 128, 760
MaeIII GTNAC 4 cut(s) 227, 427, 473, 872
MalI GATC 6 cut(s) 27, 481, 600, 774, 780, 833
MbiI CCGCTC 1 cut(s) 181
MboI GATC 6 cut(s) 25, 479, 598, 772, 778, 831
MboII GAAGA 5 cut(s) 20, 300, 650, 682, 742
MflI RGATCY 2 cut(s) 25, 772
MhlI GDGCHC 2 cut(s) 441, 1004
MluCI AATT 3 cut(s) 154, 527, 725
Mly113I GGCGCC 1 cut(s) 248
MlyI GAGTC 2 cut(s) 340, 1043
MmeI TCCRAC 1 cut(s) 864
MroNI GCCGGC 2 cut(s) 52, 1176
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 858
MspI CCGG 7 cut(s) 53, 261, 297, 309, 328, 1104, 1177
MspR9I CCNGG 8 cut(s) 16, 133, 262, 270, 310, 491, 578, 613
MvaI CCWGG 6 cut(s) 16, 133, 270, 491, 578, 613
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 6 cut(s) 49, 58, 355, 364, 710, 1119
NaeI GCCGGC 2 cut(s) 54, 1178
NarI GGCGCC 1 cut(s) 248
NciI CCSGG 2 cut(s) 262, 310
NcoI CCATGG 1 cut(s) 92
NdeII GATC 6 cut(s) 25, 479, 598, 772, 778, 831
NgoMIV GCCGGC 2 cut(s) 52, 1176
NheI GCTAGC 1 cut(s) 899
NlaIII CATG 4 cut(s) 60, 96, 849, 1020
NlaIV GGNNCC 6 cut(s) 36, 249, 267, 808, 852, 853
NmuCI GTSAC 2 cut(s) 427, 473
NotI GCGGCCGC 1 cut(s) 86
NspI RCATGY 1 cut(s) 1020
PaeR7I CTCGAG 1 cut(s) 582
PceI AGGCCT 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdiI GCCGGC 2 cut(s) 54, 1178
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 186
PinAI ACCGGT 1 cut(s) 296
PkrI GCNGC 5 cut(s) 87, 90, 366, 548, 1070
Ple19I CGATCG 1 cut(s) 834
PleI GAGTC 2 cut(s) 339, 1042
PluTI GGCGCC 1 cut(s) 251
PpsI GAGTC 2 cut(s) 339, 1042
PpuMI RGGWCCY 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 851
Psp6I CCWGG 6 cut(s) 14, 131, 268, 489, 576, 611
PspFI CCCAGC 3 cut(s) 381, 854, 858
PspGI CCWGG 6 cut(s) 14, 131, 268, 489, 576, 611
PspN4I GGNNCC 6 cut(s) 36, 249, 267, 808, 852, 853
PspPI GGNCC 6 cut(s) 35, 60, 96, 258, 609, 851
PspPPI RGGWCCY 1 cut(s) 851
PstNI CAGNNNCTG 2 cut(s) 612, 1068
PsuI RGATCY 2 cut(s) 25, 772
PvuI CGATCG 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 858
RsaI GTAC 4 cut(s) 301, 808, 992, 1082
RsaNI GTAC 4 cut(s) 300, 807, 991, 1081
SacI GAGCTC 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 283
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 5 cut(s) 86, 89, 365, 547, 1069
Sau3AI GATC 6 cut(s) 25, 479, 598, 772, 778, 831
Sau96I GGNCC 6 cut(s) 35, 60, 96, 258, 609, 851
ScaI AGTACT 1 cut(s) 992
SchI GAGTC 2 cut(s) 340, 1043
ScrFI CCNGG 8 cut(s) 16, 133, 262, 270, 310, 491, 578, 613
SduI GDGCHC 2 cut(s) 441, 1004
SfaNI GCATC 3 cut(s) 485, 544, 1090
SfiI GGCCNNNNNGGCC 2 cut(s) 49, 58
SfoI GGCGCC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 582
SinI GGWCC 3 cut(s) 96, 258, 851
SlaI CTCGAG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 21, 582
SmoI CTYRAG 2 cut(s) 21, 582
SpeI ACTAGT 1 cut(s) 463
Sse9I AATT 3 cut(s) 154, 527, 725
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 5 cut(s) 85, 89, 181, 547, 1186
SspDI GGCGCC 1 cut(s) 247
SspMI CTAG 6 cut(s) 464, 776, 900, 1031, 1167, 1194
SstI GAGCTC 1 cut(s) 441
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 8 cut(s) 14, 131, 260, 268, 308, 489, 576, 611
StyI CCWWGG 3 cut(s) 92, 251, 1193
TaaI ACNGT 7 cut(s) 233, 397, 406, 785, 979, 1064, 1087
TaiI ACGT 2 cut(s) 131, 763
TaqI TCGA 7 cut(s) 110, 189, 219, 283, 338, 583, 830
TaqII GACCGA 1 cut(s) 269
TasI AATT 3 cut(s) 154, 527, 725
TatI WGTACW 2 cut(s) 990, 1080
TauI GCSGC 3 cut(s) 88, 91, 549
TfiI GAWTC 1 cut(s) 186
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 2 cut(s) 411, 760
TseFI GTSAC 2 cut(s) 427, 473
TseI GCWGC 2 cut(s) 364, 1068
Tsp45I GTSAC 2 cut(s) 427, 473
TspDTI ATGAA 5 cut(s) 17, 127, 205, 520, 651
TspGWI ACGGA 2 cut(s) 324, 904
TspRI CASTG 2 cut(s) 411, 760
VpaK11BI GGWCC 3 cut(s) 96, 258, 851
XapI RAATTY 1 cut(s) 725
XbaI TCTAGA 2 cut(s) 775, 1030
XceI RCATGY 1 cut(s) 1020
XcmI CCANNNNNNNNNTGG 3 cut(s) 45, 497, 584
XhoI CTCGAG 1 cut(s) 582
XmaJI CCTAGG 1 cut(s) 1193
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 6 cut(s) 464, 776, 900, 1031, 1167, 1194
ZrmI AGTACT 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.