pycom10g09270

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
12276730 .. 12279507
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGAAGTTAAAACTGCCTATATTTGGTCGATTTGCAATACTGTTTTCAGTTGCAATTGTATGGATATATGCAGAAGTTTTGACTGCAGCTGATGCGTATAAGAAAAGACCTTATACAACTCAAACTATTTGCCGTACTGATCGTTCTGGGCTCATTAGTGCTTCTCCTTGGATAAGGATTCCCTATCCATTTCAATGGGGGCATCCTGATTTTAATGCTGGAGATACTTTTGCAATGATGGCTGCGGCTTTGGTTGCCATTATTGAGGGCGTAAGCACTTTTCTGGATGGCATATTTGGCTCAGTATCTGGCTCCTCTGCTTCAGTAGTAATCCAGATATCAGCAGCCTTTATGCTCTTCTTCTCTGTATTAGCTTCTGCTGGTCTTGGTTTCCTCCAGTTCTGCAACCTAAACAGCTTTCGGACAAAGTTCATTGTAGGCTTTTCTCTCTTCATGGGTCTTTCGGTGACGGAATTCTTCCCTGAATACCTCCTAATTTCCGATCGCGGTCCTGTTCACACTCGTTCCATTTGGGTGATTCAAGTAATTTTCTCGTCCCCAGCAACCGTTGGCATCATTGTTGCTTTCCTCTTGGACTTAACTGTCAGTCGTGGACACAGTGCAACTCGGAGAGACAGTGGCAGGCACTGGTGGGAAAAGTTCCAGAATTTGAACACAGATACCAGGAGTGAAGAGTTCTACTCACTGCCCTACAACCTCAACAGGCATTTCCCTTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

248

Amino Acids

27.61

Weight (kDa)

9.2

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 6 - 93 1.8e-09 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 602
AccII CGCG 1 cut(s) 507
AciI CCGC 2 cut(s) 245, 507
AcsI RAATTY 2 cut(s) 473, 667
AcuI CTGAAG 2 cut(s) 306, 720
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 23
AgsI TTSAA 3 cut(s) 194, 542, 673
AjnI CCWGG 1 cut(s) 683
AluBI AGCT 3 cut(s) 89, 374, 417
AluI AGCT 3 cut(s) 89, 374, 417
Alw26I GTCTC 1 cut(s) 627
AlwNI CAGNNNCTG 2 cut(s) 308, 648
ApeKI GCWGC 3 cut(s) 86, 242, 344
ApoI RAATTY 2 cut(s) 473, 667
AspS9I GGNCC 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 478, 547
AvaII GGWCC 1 cut(s) 509
BanII GRGCYC 1 cut(s) 153
BbvI GCAGC 3 cut(s) 98, 229, 356
BccI CCATC 2 cut(s) 232, 281
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 1 cut(s) 685
BcoDI GTCTC 1 cut(s) 627
BfmI CTRYAG 1 cut(s) 84
BisI GCNGC 4 cut(s) 87, 243, 246, 345
BlsI GCNGC 4 cut(s) 88, 244, 247, 346
Bme1390I CCNGG 1 cut(s) 685
Bme18I GGWCC 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 509
BmiI GGNNCC 1 cut(s) 313
BmrFI CCNGG 1 cut(s) 685
BmsI GCATC 3 cut(s) 82, 211, 582
BplI GAGNNNNNCTC 2 cut(s) 686, 718
BpmI CTGGAG 2 cut(s) 240, 380
BsaJI CCNNGG 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse1I ACTGG 2 cut(s) 397, 653
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 2 cut(s) 202, 292
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 2 cut(s) 397, 653
BseRI GAGGAG 1 cut(s) 304
BseXI GCAGC 3 cut(s) 98, 229, 356
BseYI CCCAGC 1 cut(s) 559
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 505
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 1 cut(s) 23
BsmAI GTCTC 1 cut(s) 627
BsmFI GGGAC 1 cut(s) 541
Bsp1286I GDGCHC 1 cut(s) 153
Bsp143I GATC 2 cut(s) 139, 502
BspACI CCGC 2 cut(s) 245, 507
BspCNI CTCAG 1 cut(s) 314
BspFNI CGCG 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 313
BspMAI CTGCAG 1 cut(s) 88
BspQI GCTCTTC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 2 cut(s) 397, 653
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 2 cut(s) 139, 502
BssT1I CCWWGG 1 cut(s) 167
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 5 cut(s) 42, 568, 604, 620, 638
Bst6I CTCTTC 3 cut(s) 362, 455, 687
BstAPI GCANNNNNTGC 1 cut(s) 92
BstC8I GCNNGC 1 cut(s) 644
BstDEI CTNAG 1 cut(s) 301
BstF5I GGATG 2 cut(s) 202, 292
BstFNI CGCG 1 cut(s) 507
BstKTI GATC 2 cut(s) 142, 505
BstMAI GTCTC 1 cut(s) 627
BstMBI GATC 2 cut(s) 139, 502
BstMCI CGRYCG 1 cut(s) 505
BstMWI GCNNNNNNNGC 4 cut(s) 92, 239, 254, 297
BstNI CCWGG 1 cut(s) 685
BstSCI CCNGG 1 cut(s) 683
BstSFI CTRYAG 1 cut(s) 84
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 3 cut(s) 98, 229, 356
BstXI CCANNNNNNTGG 1 cut(s) 195
BtsCI GGATG 2 cut(s) 202, 292
BtsI GCAGTG 1 cut(s) 704
BtsIMutI CAGTG 4 cut(s) 625, 643, 646, 704
Cac8I GCNNGC 1 cut(s) 644
CaiI CAGNNNCTG 2 cut(s) 308, 648
Cfr13I GGNCC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 135
CviAII CATG 1 cut(s) 454
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 1 cut(s) 301
DpnI GATC 2 cut(s) 141, 504
DpnII GATC 2 cut(s) 139, 502
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
Eam1104I CTCTTC 3 cut(s) 362, 455, 687
EarI CTCTTC 3 cut(s) 362, 455, 687
Eco130I CCWWGG 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 153
Eco32I GATATC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 509
Eco57I CTGAAG 2 cut(s) 306, 720
EcoRI GAATTC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 683
EcoRV GATATC 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 1 cut(s) 457
FaiI YATR 9 cut(s) 20, 61, 67, 69, 99, 114, 293, 353, 455
FaqI GGGAC 1 cut(s) 541
FatI CATG 1 cut(s) 453
Fnu4HI GCNGC 4 cut(s) 87, 243, 246, 345
FokI GGATG 2 cut(s) 189, 299
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 4 cut(s) 87, 243, 246, 345
GluI GCNGC 4 cut(s) 87, 243, 246, 345
GsaI CCCAGC 1 cut(s) 563
GsuI CTGGAG 2 cut(s) 240, 380
Hin1II CATG 1 cut(s) 457
HinfI GANTC 2 cut(s) 178, 538
HphI GGTGA 2 cut(s) 478, 547
Hpy166II GTNNAC 2 cut(s) 517, 614
Hpy188I TCNGA 3 cut(s) 423, 502, 630
Hpy188III TCNNGA 4 cut(s) 206, 284, 334, 664
Hpy8I GTNNAC 2 cut(s) 517, 614
HpyAV CCTTC 1 cut(s) 744
HpyCH4III ACNGT 5 cut(s) 42, 568, 604, 620, 638
HpyCH4V TGCA 7 cut(s) 35, 53, 71, 86, 233, 405, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 239, 254, 297
HpyF3I CTNAG 1 cut(s) 301
Hsp92II CATG 1 cut(s) 457
Kzo9I GATC 2 cut(s) 139, 502
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 1 cut(s) 317
Lsp1109I GCAGC 3 cut(s) 98, 229, 356
LweI GCATC 3 cut(s) 82, 211, 582
MaeIII GTNAC 1 cut(s) 466
MalI GATC 2 cut(s) 141, 504
MboI GATC 2 cut(s) 139, 502
MboII GAAGA 5 cut(s) 349, 352, 442, 469, 704
MfeI CAATTG 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 153
MluCI AATT 5 cut(s) 54, 473, 495, 546, 667
MnlI CCTC 6 cut(s) 259, 325, 404, 500, 599, 728
MseI TTAA 3 cut(s) 8, 213, 599
MslI CAYNNNNRTG 2 cut(s) 193, 533
MspA1I CMGCKG 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 685
MunI CAATTG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 4 cut(s) 92, 239, 254, 297
NdeII GATC 2 cut(s) 139, 502
NlaIII CATG 1 cut(s) 457
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 362
PfeI GAWTC 2 cut(s) 178, 538
PkrI GCNGC 4 cut(s) 88, 244, 247, 346
Ple19I CGATCG 1 cut(s) 505
Psp6I CCWGG 1 cut(s) 683
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 1 cut(s) 509
PsrI GAACNNNNNNTAC 2 cut(s) 127, 159
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 2 cut(s) 308, 648
PvuI CGATCG 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 2 cut(s) 193, 533
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 3 cut(s) 8, 213, 599
SatI GCNGC 4 cut(s) 87, 243, 246, 345
Sau3AI GATC 2 cut(s) 139, 502
Sau96I GGNCC 1 cut(s) 509
ScrFI CCNGG 1 cut(s) 685
SduI GDGCHC 1 cut(s) 153
SetI ASST 7 cut(s) 91, 112, 376, 411, 419, 492, 720
SfaNI GCATC 3 cut(s) 82, 211, 582
SfcI CTRYAG 1 cut(s) 84
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 2 cut(s) 193, 533
Sse9I AATT 5 cut(s) 54, 473, 495, 546, 667
SsiI CCGC 2 cut(s) 245, 507
StyD4I CCNGG 1 cut(s) 683
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 5 cut(s) 42, 568, 604, 620, 638
TaqI TCGA 1 cut(s) 28
TasI AATT 5 cut(s) 54, 473, 495, 546, 667
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 2 cut(s) 178, 538
Tru1I TTAA 3 cut(s) 8, 213, 599
Tru9I TTAA 3 cut(s) 8, 213, 599
TscAI CASTG 4 cut(s) 625, 643, 653, 711
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 3 cut(s) 86, 242, 344
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 3 cut(s) 17, 421, 442
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 4 cut(s) 625, 643, 653, 711
VpaK11BI GGWCC 1 cut(s) 509
XapI RAATTY 2 cut(s) 473, 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.