pycom10g10430

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
13703785 .. 13704919
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGGGTTCCGAGGTCTCGAAACAGGTAGAACGGAGAAAGGCGATCGCGGCGGAGAAAAAAACACTGTGTGATCTTCACCAGAGCTGTGGTGAAGAGTTTCCATCATGTGCCTACAAGCCTTCAGACAGAAAAAATTGGATGGCTGGGGTCAACCCTGAGAAACTTCACATAAACAAGATCGTATGGCCGGGAACTCACGACTCTGCAACCAACAAGATTGGAGTTCCATGCATCACTAGGCCTTTTGCTCAATGCCAAAATCTCTCCATCTACCAACAGCTCGAGAAAGGCAATAGAGTTCTAGATATTCGAATCCAGGAGGACCGAAAAGTCTGCCACGGAATCCTCCTCACTTACAACATTGATGTTGTGATCAACGACGTCAAGAAGTTTTTATCGGAAACGCAGTATGAGATCATAATACTCGAAATCCGAACTGAATTTGGACATGAAGATCCACCTGAGTTTGATAAGTACTTGATCGATCAGCTCGGCGAGTTTTTAGTCCCCCAGGACGATCATGTTTTCGACAAGCCTATTTCAGAACTGCTGCCAAAGAGAGTGATATGCGTGTGGAAGCCGAGGAAGTCACCTCAGCCAAAGGCAGGGAGTCCACTGTGGAATGCAGGGCATTTGAAGGACGACTGGATTGACACGGATTTGCCATCCACCAAATTCGAGAGCAACCTGAAGAATTTGAGTAATCAGCCACCGGCTTCATCAAGGAAGTTCTTTTACAGGATGAGGATTTCGTGGATGCTTGCGTTGCGGTTACATATGCAAGAGTTGAAGGGAAAGCATGAAGTTTCCATGTCTGTGCAGTCTTGTTCTTGTTTTCGTGTTGATAAATCTTCCTTCCCACCGTGTAATTCAATCCTTTCTACTTTGTTACCATTTTCTGCATCCAAAGAGCTTGCATTGTTATTCTTAAGGGATAGCGAGGATGTCAAGGTTGCGTTGTTCCGAACATATCACGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

327

Amino Acids

37.46

Weight (kDa)

8.44

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 63 - 174 3.4e-09 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AatII GACGTC 1 cut(s) 384
AccII CGCG 1 cut(s) 47
AciI CCGC 3 cut(s) 47, 50, 769
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 3 cut(s) 440, 674, 694
AcuI CTGAAG 2 cut(s) 105, 710
AcvI CACGTG 1 cut(s) 976
AcyI GRCGYC 1 cut(s) 381
AdeI CACNNNGTG 1 cut(s) 68
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 605
AflII CTTAAG 1 cut(s) 928
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 3 cut(s) 637, 790, 873
AjnI CCWGG 2 cut(s) 315, 510
AluBI AGCT 4 cut(s) 84, 280, 490, 913
AluI AGCT 4 cut(s) 84, 280, 490, 913
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 2 cut(s) 185, 239
ApeKI GCWGC 1 cut(s) 550
ApoI RAATTY 3 cut(s) 440, 674, 694
AsiSI GCGATCGC 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 96
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 3 cut(s) 68, 101, 582
AsuII TTCGAA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 322
BarI GAAGNNNNNNTAC 2 cut(s) 719, 751
BbrPI CACGTG 1 cut(s) 976
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 1 cut(s) 537
BccI CCATC 4 cut(s) 109, 133, 275, 673
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BciT130I CCWGG 2 cut(s) 317, 512
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 2 cut(s) 237, 302
BfrI CTTAAG 1 cut(s) 928
BisI GCNGC 2 cut(s) 48, 551
BlsI GCNGC 2 cut(s) 49, 552
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 3 cut(s) 189, 317, 512
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 3 cut(s) 189, 317, 512
BmsI GCATC 3 cut(s) 240, 747, 911
Bpu10I CCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 310
BpuMI CCSGG 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 483
BsaAI YACGTR 1 cut(s) 976
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 4 cut(s) 9, 337, 510, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 1 cut(s) 650
BseBI CCWGG 2 cut(s) 317, 512
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 4 cut(s) 9, 337, 510, 581
BseGI GGATG 6 cut(s) 144, 665, 747, 762, 902, 949
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 3 cut(s) 147, 453, 608
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 537
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 839
Bsh1236I CGCG 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 187, 241
BshVI ATCGAT 1 cut(s) 483
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 188, 713
BslFI GGGAC 1 cut(s) 491
BslI CCNNNNNNNGG 1 cut(s) 605
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 491
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 2 cut(s) 187, 241
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 310
Bsp143I GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
BspACI CCGC 3 cut(s) 47, 50, 769
BspANI GGCC 2 cut(s) 187, 241
BspCNI CTCAG 3 cut(s) 148, 454, 607
BspDI ATCGAT 1 cut(s) 483
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 1 cut(s) 449
BspT104I TTCGAA 1 cut(s) 310
BspTI CTTAAG 1 cut(s) 928
BspTNI GGTCTC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 4 cut(s) 9, 337, 510, 581
BssMI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 2 cut(s) 317, 512
Bst4CI ACNGT 3 cut(s) 66, 618, 864
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 381
BstAFI CTTAAG 1 cut(s) 928
BstBAI YACGTR 1 cut(s) 976
BstBI TTCGAA 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 762, 915
BstDEI CTNAG 3 cut(s) 156, 462, 594
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 6 cut(s) 144, 665, 747, 762, 902, 949
BstFNI CGCG 1 cut(s) 47
BstKTI GATC 9 cut(s) 45, 73, 180, 375, 417, 457, 483, 487, 520
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 47, 766
BstNI CCWGG 2 cut(s) 317, 512
BstSCI CCNGG 3 cut(s) 187, 315, 510
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 86
BstYI RGATCY 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 483
BsuRI GGCC 2 cut(s) 187, 241
BsuTUI ATCGAT 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 6 cut(s) 144, 665, 747, 762, 902, 949
BtsIMutI CAGTG 2 cut(s) 62, 614
Cac8I GCNNGC 2 cut(s) 762, 915
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 322
ClaI ATCGAT 1 cut(s) 483
Csp6I GTAC 1 cut(s) 475
CviAII CATG 6 cut(s) 105, 228, 449, 521, 800, 811
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 3 cut(s) 156, 462, 594
DpnI GATC 9 cut(s) 44, 72, 179, 374, 416, 456, 482, 486, 519
DpnII GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
DraIII CACNNNGTG 1 cut(s) 68
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 65
Eco147I AGGCCT 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 105, 710
Eco72I CACGTG 1 cut(s) 976
Eco88I CYCGRG 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 315, 510
EcoT22I ATGCAT 1 cut(s) 233
FaeI CATG 6 cut(s) 108, 231, 452, 524, 803, 814
FaqI GGGAC 1 cut(s) 491
FatI CATG 6 cut(s) 104, 227, 448, 520, 799, 810
FauNDI CATATG 1 cut(s) 777
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 48, 551
FokI GGATG 6 cut(s) 151, 652, 754, 769, 889, 956
Fsp4HI GCNGC 2 cut(s) 48, 551
FspBI CTAG 2 cut(s) 237, 302
GluI GCNGC 2 cut(s) 48, 551
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 187, 241
HapII CCGG 2 cut(s) 188, 713
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 6 cut(s) 108, 231, 452, 524, 803, 814
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 4 cut(s) 200, 312, 342, 610
HpaII CCGG 2 cut(s) 188, 713
HphI GGTGA 3 cut(s) 68, 101, 582
Hpy166II GTNNAC 2 cut(s) 151, 614
Hpy188I TCNGA 6 cut(s) 10, 124, 400, 434, 544, 965
Hpy188III TCNNGA 6 cut(s) 16, 197, 283, 302, 385, 679
Hpy8I GTNNAC 2 cut(s) 151, 614
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 4 cut(s) 129, 631, 784, 865
HpyCH4III ACNGT 3 cut(s) 66, 618, 864
HpyCH4IV ACGT 2 cut(s) 381, 975
HpyCH4V TGCA 7 cut(s) 206, 231, 626, 781, 820, 902, 917
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 766
HpyF3I CTNAG 3 cut(s) 156, 462, 594
HpySE526I ACGT 2 cut(s) 381, 975
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 6 cut(s) 108, 231, 452, 524, 803, 814
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
Lsp1109I GCAGC 1 cut(s) 537
LweI GCATC 3 cut(s) 240, 747, 911
MaeI CTAG 2 cut(s) 237, 302
MaeII ACGT 2 cut(s) 381, 975
MaeIII GTNAC 3 cut(s) 588, 771, 888
MalI GATC 9 cut(s) 44, 72, 179, 374, 416, 456, 482, 486, 519
MboI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
MboII GAAGA 5 cut(s) 65, 104, 464, 703, 843
MflI RGATCY 1 cut(s) 454
MluCI AATT 5 cut(s) 133, 440, 674, 694, 868
MlyI GAGTC 2 cut(s) 194, 619
MnlI CCTC 8 cut(s) 4, 313, 356, 359, 576, 603, 738, 934
Mph1103I ATGCAT 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 1 cut(s) 929
MslI CAYNNNNRTG 1 cut(s) 815
MspCI CTTAAG 1 cut(s) 928
MspI CCGG 2 cut(s) 188, 713
MspR9I CCNGG 3 cut(s) 189, 317, 512
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 2 cut(s) 317, 512
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 2 cut(s) 47, 766
NciI CCSGG 1 cut(s) 189
NdeI CATATG 1 cut(s) 777
NdeII GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
NlaIII CATG 6 cut(s) 108, 231, 452, 524, 803, 814
NlaIV GGNNCC 1 cut(s) 7
NmeAIII GCCGAG 2 cut(s) 471, 606
NmuCI GTSAC 1 cut(s) 588
NsiI ATGCAT 1 cut(s) 233
NspV TTCGAA 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 281
PceI AGGCCT 1 cut(s) 241
PctI GAATGC 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 2 cut(s) 312, 342
PfoI TCCNGGA 1 cut(s) 315
PkrI GCNGC 2 cut(s) 49, 552
Ple19I CGATCG 1 cut(s) 45
PleI GAGTC 2 cut(s) 194, 618
PmaCI CACGTG 1 cut(s) 976
PmlI CACGTG 1 cut(s) 976
PpsI GAGTC 2 cut(s) 194, 618
Ppu21I YACGTR 1 cut(s) 976
Psp6I CCWGG 2 cut(s) 315, 510
PspCI CACGTG 1 cut(s) 976
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 315, 510
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 322
PsuI RGATCY 1 cut(s) 454
PvuI CGATCG 1 cut(s) 45
RgaI GCGATCGC 1 cut(s) 45
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 815
SaqAI TTAA 1 cut(s) 929
SatI GCNGC 2 cut(s) 48, 551
Sau3AI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 480, 484, 517
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 2 cut(s) 194, 619
ScrFI CCNGG 3 cut(s) 189, 317, 512
SfaAI GCGATCGC 1 cut(s) 45
SfaNI GCATC 3 cut(s) 240, 747, 911
Sfr274I CTCGAG 1 cut(s) 281
SfuI TTCGAA 1 cut(s) 310
SgfI GCGATCGC 1 cut(s) 45
SinI GGWCC 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 815
SmlI CTYRAG 2 cut(s) 281, 928
SmoI CTYRAG 2 cut(s) 281, 928
Sse9I AATT 5 cut(s) 133, 440, 674, 694, 868
SseBI AGGCCT 1 cut(s) 241
SsiI CCGC 3 cut(s) 47, 50, 769
SspMI CTAG 2 cut(s) 237, 302
StuI AGGCCT 1 cut(s) 241
StyD4I CCNGG 3 cut(s) 187, 315, 510
TaaI ACNGT 3 cut(s) 66, 618, 864
TaiI ACGT 2 cut(s) 384, 978
TaqI TCGA 7 cut(s) 17, 282, 310, 426, 483, 528, 678
TaqII GACCGA 1 cut(s) 339
TasI AATT 5 cut(s) 133, 440, 674, 694, 868
TatI WGTACW 1 cut(s) 474
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 312, 342
Tru1I TTAA 1 cut(s) 929
Tru9I TTAA 1 cut(s) 929
TscAI CASTG 2 cut(s) 69, 621
TseFI GTSAC 1 cut(s) 588
TseI GCWGC 1 cut(s) 550
Tsp45I GTSAC 1 cut(s) 588
TspDTI ATGAA 3 cut(s) 465, 708, 816
TspGWI ACGGA 3 cut(s) 46, 354, 671
TspRI CASTG 2 cut(s) 69, 621
Vha464I CTTAAG 1 cut(s) 928
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 3 cut(s) 440, 674, 694
XbaI TCTAGA 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 2 cut(s) 237, 302
ZraI GACGTC 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 476
Zsp2I ATGCAT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.