pycom10g12040

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
15651441 .. 15651941
501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ATGGAACATCTGAACTGTCTTAGTTTGAAAAAGACAGCGGTCAAAGAGCTCCCTTCGTCTATTGGAAATCTAATTGGGCTTGAAACAGTAGATCTTGATATGTGCAGGAACCTTAATGTTGTCCCAAGAAGTATCTACAGTTTAACCAACCTTAAAACTCTCAGATTTTGTTGCTGTCACGAAATCAAAGAATTTCCGTCTGGCTCTATTCATTTGCTATCTTTGAAAGTTCTAGACCTCCATGGCACCCGCATATCAGAAATCCCTGATGGTCTCGTTGGCTCAACCTCATTGCAAGATTTGGATCTAAGTAGAAGCACGATCAGGAGCATACCTGCAAGCATCAAACATGCTTCTCAGTTGCATAGACTAAGCTTAATTGGGTGCAAGCGGCTTCAATCATTACCAGAGCTCCCAGTGGATCAAGTTATAAGAATAAAGCAAATGAAAGTGATGATGATTCAGAAGCCAGTGAATAAAGTTATGATCAGTTTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

167

Amino Acids

18.49

Weight (kDa)

9.28

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 3 - 75 2.8e-10 Leucine-rich repeat region
LRR_13 PF23286 9 - 145 5e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 49 - 105 1.6e-06 Leucine rich repeat
LRR_14 PF23598 71 - 148 1.8e-07 Leucine-rich repeat region
LRR_8 PF13855 74 - 129 1.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 245
AciI CCGC 3 cut(s) 38, 250, 391
AclWI GGATC 2 cut(s) 312, 429
AcsI RAATTY 1 cut(s) 191
AgsI TTSAA 4 cut(s) 28, 83, 226, 398
AluBI AGCT 3 cut(s) 49, 375, 412
AluI AGCT 3 cut(s) 49, 375, 412
Alw21I GWGCWC 2 cut(s) 51, 414
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 2 cut(s) 312, 429
ApoI RAATTY 1 cut(s) 191
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 2 cut(s) 51, 414
Bbv12I GWGCWC 2 cut(s) 51, 414
BccI CCATC 1 cut(s) 263
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 343
BglII AGATCT 1 cut(s) 91
BisI GCNGC 1 cut(s) 392
BlsI GCNGC 1 cut(s) 393
BmiI GGNNCC 2 cut(s) 110, 247
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 1 cut(s) 351
BmuI ACTGGG 1 cut(s) 410
BoxI GACNNNNGTC 1 cut(s) 38
BsaBI GATNNNNATC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bse1I ACTGG 2 cut(s) 416, 470
Bse3DI GCAATG 1 cut(s) 290
Bse8I GATNNNNATC 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 241
BseJI GATNNNNATC 1 cut(s) 303
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 175, 371
BseNI ACTGG 2 cut(s) 416, 470
BsgI GTGCAG 1 cut(s) 124
BshNI GGYRCC 1 cut(s) 245
BsiHKAI GWGCWC 2 cut(s) 51, 414
BslFI GGGAC 1 cut(s) 107
BsmAI GTCTC 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 107
Bso31I GGTCTC 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 51, 414
Bsp143I GATC 5 cut(s) 91, 304, 321, 421, 486
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 3 cut(s) 38, 250, 391
BspCNI CTCAG 2 cut(s) 174, 370
BspLI GGNNCC 2 cut(s) 110, 247
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 2 cut(s) 312, 429
BspT107I GGYRCC 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 290
BsrI ACTGG 2 cut(s) 416, 470
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 5 cut(s) 91, 304, 321, 421, 486
BssT1I CCWWGG 1 cut(s) 241
Bst4CI ACNGT 3 cut(s) 17, 88, 140
BstC8I GCNNGC 2 cut(s) 340, 389
BstDEI CTNAG 5 cut(s) 20, 161, 308, 357, 371
BstDSI CCRYGG 1 cut(s) 241
BstKTI GATC 5 cut(s) 94, 307, 324, 424, 489
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 5 cut(s) 91, 304, 321, 421, 486
BstNSI RCATGY 1 cut(s) 353
BstPAI GACNNNNGTC 1 cut(s) 38
BstSFI CTRYAG 1 cut(s) 136
BstX2I RGATCY 2 cut(s) 91, 304
BstYI RGATCY 2 cut(s) 91, 304
BtgI CCRYGG 1 cut(s) 241
BtsIMutI CAGTG 2 cut(s) 423, 477
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 2 cut(s) 340, 389
CviAII CATG 2 cut(s) 242, 350
CviJI RGCY 8 cut(s) 49, 79, 204, 282, 375, 394, 412, 469
CviKI_1 RGCY 8 cut(s) 49, 79, 204, 282, 375, 394, 412, 469
DdeI CTNAG 5 cut(s) 20, 161, 308, 357, 371
DpnI GATC 5 cut(s) 93, 306, 323, 423, 488
DpnII GATC 5 cut(s) 91, 304, 321, 421, 486
Ecl136II GAGCTC 2 cut(s) 49, 412
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 2 cut(s) 51, 414
Eco31I GGTCTC 1 cut(s) 278
Eco53kI GAGCTC 2 cut(s) 49, 412
EcoICRI GAGCTC 2 cut(s) 49, 412
EcoT14I CCWWGG 1 cut(s) 241
EcoT38I GRGCYC 2 cut(s) 51, 414
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 2 cut(s) 245, 353
FaiI YATR 8 cut(s) 101, 243, 254, 332, 351, 366, 431, 485
FaqI GGGAC 1 cut(s) 107
FatI CATG 2 cut(s) 241, 349
FauI CCCGC 1 cut(s) 257
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 1 cut(s) 392
FriOI GRGCYC 2 cut(s) 51, 414
Fsp4HI GCNGC 1 cut(s) 392
FspBI CTAG 1 cut(s) 233
GluI GCNGC 1 cut(s) 392
Hin1II CATG 2 cut(s) 245, 353
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 1 cut(s) 460
Hpy188I TCNGA 4 cut(s) 12, 164, 259, 465
Hpy188III TCNNGA 4 cut(s) 95, 179, 233, 325
HpyAV CCTTC 1 cut(s) 63
HpyCH4III ACNGT 3 cut(s) 17, 88, 140
HpyCH4V TGCA 5 cut(s) 105, 295, 338, 364, 387
HpyF3I CTNAG 5 cut(s) 20, 161, 308, 357, 371
Hsp92II CATG 2 cut(s) 245, 353
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 5 cut(s) 91, 304, 321, 421, 486
LmnI GCTCC 3 cut(s) 54, 327, 417
LpnPI CCDG 8 cut(s) 91, 186, 279, 310, 348, 420, 429, 483
LweI GCATC 1 cut(s) 351
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 176
MalI GATC 5 cut(s) 93, 306, 323, 423, 488
MboI GATC 5 cut(s) 91, 304, 321, 421, 486
MflI RGATCY 2 cut(s) 91, 304
MhlI GDGCHC 2 cut(s) 51, 414
MluCI AATT 3 cut(s) 72, 191, 378
MnlI CCTC 2 cut(s) 248, 298
MseI TTAA 5 cut(s) 114, 143, 153, 377, 499
MspA1I CMGCKG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 241
NdeII GATC 5 cut(s) 91, 304, 321, 421, 486
NlaIII CATG 2 cut(s) 245, 353
NlaIV GGNNCC 2 cut(s) 110, 247
NmuCI GTSAC 1 cut(s) 176
NspI RCATGY 1 cut(s) 353
PfeI GAWTC 1 cut(s) 460
PkrI GCNGC 1 cut(s) 393
PshAI GACNNNNGTC 1 cut(s) 38
PsiI TTATAA 1 cut(s) 431
Psp124BI GAGCTC 2 cut(s) 51, 414
PspN4I GGNNCC 2 cut(s) 110, 247
PsuI RGATCY 2 cut(s) 91, 304
SacI GAGCTC 2 cut(s) 51, 414
SaqAI TTAA 5 cut(s) 114, 143, 153, 377, 499
SatI GCNGC 1 cut(s) 392
Sau3AI GATC 5 cut(s) 91, 304, 321, 421, 486
SduI GDGCHC 2 cut(s) 51, 414
SetI ASST 8 cut(s) 51, 114, 153, 240, 290, 337, 377, 414
SfaNI GCATC 1 cut(s) 351
SfcI CTRYAG 1 cut(s) 136
Sse9I AATT 3 cut(s) 72, 191, 378
SsiI CCGC 3 cut(s) 38, 250, 391
SspMI CTAG 1 cut(s) 233
SstI GAGCTC 2 cut(s) 51, 414
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 3 cut(s) 17, 88, 140
TasI AATT 3 cut(s) 72, 191, 378
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 5 cut(s) 114, 143, 153, 377, 499
Tru9I TTAA 5 cut(s) 114, 143, 153, 377, 499
TscAI CASTG 2 cut(s) 423, 477
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 2 cut(s) 200, 461
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 2 cut(s) 423, 477
XapI RAATTY 1 cut(s) 191
XbaI TCTAGA 1 cut(s) 232
XceI RCATGY 1 cut(s) 353
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.