pycom10g12660

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
16127476 .. 16127877
402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
ATGAGCGGTGATTGGCTATGCGCGGTGATACTCTCATATCTTGGGACAGATCTCGAAGCTATGGGGGCGACGATACTCCCAAATCTCGGGGCGGAGATTTTGATTCCACTGTGCGCGGTGATCAGAATCGCCTTCTCCTTGGTCCAGTGGATGTACGTTTCGCAGGTTAAGCTCTCCCTAGGTAGCGGTCGAGACTCCAATTCTAGTAACGCTGGCAAAAATGGGTACAACGATTACCTCATCGAGGAGGAAGAAGGAGTGATGATCACAATGTCATCCTCAGATGTGCCGAAATCCAAACCGCCATTTTCGAAGGTCCATTTCCACAACTCTGAAATTTTGATTGATAAAACGGCGTCAAATTTGTTCATTTGGTTTTCTGGGTTCGGTTTATGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

134

Amino Acids

14.46

Weight (kDa)

4.8

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 2 cut(s) 23, 116
AciI CCGC 6 cut(s) 6, 23, 92, 116, 186, 302
AcsI RAATTY 2 cut(s) 336, 361
AcyI GRCGYC 1 cut(s) 356
AfaI GTAC 2 cut(s) 155, 227
AfiI CCNNNNNNNGG 2 cut(s) 86, 244
AluBI AGCT 2 cut(s) 59, 172
AluI AGCT 2 cut(s) 59, 172
Alw26I GTCTC 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 86
ApoI RAATTY 2 cut(s) 336, 361
AspA2I CCTAGG 1 cut(s) 178
AspLEI GCGC 2 cut(s) 23, 116
AspS9I GGNCC 2 cut(s) 142, 316
AsuHPI GGTGA 3 cut(s) 20, 37, 130
AsuII TTCGAA 1 cut(s) 311
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 2 cut(s) 142, 316
AvrII CCTAGG 1 cut(s) 178
BceAI ACGGC 1 cut(s) 369
BclI TGATCA 2 cut(s) 120, 264
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 2 cut(s) 179, 204
BfuAI ACCTGC 1 cut(s) 154
BglII AGATCT 1 cut(s) 49
BlnI CCTAGG 1 cut(s) 178
Bme18I GGWCC 2 cut(s) 142, 316
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 2 cut(s) 142, 316
Bpu14I TTCGAA 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 2 cut(s) 138, 178
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 244
Bse1I ACTGG 1 cut(s) 145
Bse8I GATNNNNATC 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 138, 178
BseGI GGATG 2 cut(s) 156, 275
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 2 cut(s) 86, 244
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 1 cut(s) 260
Bsh1236I CGCG 2 cut(s) 23, 116
Bsh1285I CGRYCG 1 cut(s) 190
BsiEI CGRYCG 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 86
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 86, 244
BsmAI GTCTC 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 311
Bsp143I GATC 3 cut(s) 49, 120, 264
BspACI CCGC 6 cut(s) 6, 23, 92, 116, 186, 302
BspCNI CTCAG 1 cut(s) 293
BspFNI CGCG 2 cut(s) 23, 116
BspMI ACCTGC 1 cut(s) 154
BspT104I TTCGAA 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 6
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 138, 178
BssMI GATC 3 cut(s) 49, 120, 264
BssNI GRCGYC 1 cut(s) 356
BssT1I CCWWGG 2 cut(s) 138, 178
Bst4CI ACNGT 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 1 cut(s) 280
BstENI CCTNNNNNAGG 1 cut(s) 242
BstF5I GGATG 2 cut(s) 156, 275
BstFNI CGCG 2 cut(s) 23, 116
BstHHI GCGC 2 cut(s) 23, 116
BstKTI GATC 3 cut(s) 52, 123, 267
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 3 cut(s) 49, 120, 264
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 65, 169
BstUI CGCG 2 cut(s) 23, 116
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BtsCI GGATG 2 cut(s) 156, 275
BtsIMutI CAGTG 2 cut(s) 107, 152
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 214
CfoI GCGC 2 cut(s) 23, 116
Cfr13I GGNCC 2 cut(s) 142, 316
CseI GACGC 1 cut(s) 345
Csp6I GTAC 2 cut(s) 154, 226
CviJI RGCY 3 cut(s) 16, 59, 172
CviKI_1 RGCY 3 cut(s) 16, 59, 172
CviQI GTAC 2 cut(s) 154, 226
DdeI CTNAG 1 cut(s) 280
DpnI GATC 3 cut(s) 51, 122, 266
DpnII GATC 3 cut(s) 49, 120, 264
EciI GGCGGA 1 cut(s) 107
Eco130I CCWWGG 2 cut(s) 138, 178
Eco47I GGWCC 2 cut(s) 142, 316
Eco88I CYCGRG 1 cut(s) 86
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 138, 178
ErhI CCWWGG 2 cut(s) 138, 178
FaiI YATR 4 cut(s) 19, 37, 62, 394
FaqI GGGAC 1 cut(s) 58
FbaI TGATCA 2 cut(s) 120, 264
FokI GGATG 2 cut(s) 163, 262
FspBI CTAG 2 cut(s) 179, 204
GlaI GCGC 2 cut(s) 22, 115
HgaI GACGC 1 cut(s) 345
HhaI GCGC 2 cut(s) 23, 116
Hin1I GRCGYC 1 cut(s) 356
Hin6I GCGC 2 cut(s) 21, 114
HinP1I GCGC 2 cut(s) 21, 114
HinfI GANTC 3 cut(s) 103, 126, 194
HphI GGTGA 3 cut(s) 20, 37, 130
Hpy188I TCNGA 3 cut(s) 125, 283, 334
Hpy188III TCNNGA 2 cut(s) 53, 191
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 142, 248, 307
HpyCH4III ACNGT 1 cut(s) 111
HpyCH4IV ACGT 1 cut(s) 156
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 169
HpyF3I CTNAG 1 cut(s) 280
HpySE526I ACGT 1 cut(s) 156
Hsp92I GRCGYC 1 cut(s) 356
HspAI GCGC 2 cut(s) 21, 114
Ksp22I TGATCA 2 cut(s) 120, 264
Kzo9I GATC 3 cut(s) 49, 120, 264
LpnPI CCDG 4 cut(s) 149, 158, 198, 366
MaeI CTAG 2 cut(s) 179, 204
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 206
MalI GATC 3 cut(s) 51, 122, 266
MbiI CCGCTC 1 cut(s) 6
MboI GATC 3 cut(s) 49, 120, 264
MboII GAAGA 1 cut(s) 263
MflI RGATCY 1 cut(s) 49
MluCI AATT 3 cut(s) 199, 336, 361
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 4 cut(s) 238, 241, 248, 289
MseI TTAA 2 cut(s) 168, 400
MvnI CGCG 2 cut(s) 23, 116
MwoI GCNNNNNNNGC 2 cut(s) 65, 169
NdeII GATC 3 cut(s) 49, 120, 264
NspV TTCGAA 1 cut(s) 311
PfeI GAWTC 2 cut(s) 103, 126
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PspPI GGNCC 2 cut(s) 142, 316
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 2 cut(s) 155, 227
RsaNI GTAC 2 cut(s) 154, 226
SaqAI TTAA 2 cut(s) 168, 400
Sau3AI GATC 3 cut(s) 49, 120, 264
Sau96I GGNCC 2 cut(s) 142, 316
SchI GAGTC 1 cut(s) 188
SetI ASST 7 cut(s) 61, 159, 168, 174, 184, 240, 318
SfuI TTCGAA 1 cut(s) 311
SinI GGWCC 2 cut(s) 142, 316
Sse9I AATT 3 cut(s) 199, 336, 361
SsiI CCGC 6 cut(s) 6, 23, 92, 116, 186, 302
SspMI CTAG 2 cut(s) 179, 204
StyI CCWWGG 2 cut(s) 138, 178
TaaI ACNGT 1 cut(s) 111
TaiI ACGT 1 cut(s) 159
TaqI TCGA 4 cut(s) 54, 190, 243, 311
TasI AATT 3 cut(s) 199, 336, 361
TfiI GAWTC 2 cut(s) 103, 126
Tru1I TTAA 2 cut(s) 168, 400
Tru9I TTAA 2 cut(s) 168, 400
TscAI CASTG 2 cut(s) 114, 152
TspDTI ATGAA 1 cut(s) 358
TspRI CASTG 2 cut(s) 114, 152
VpaK11BI GGWCC 2 cut(s) 142, 316
XagI CCTNNNNNAGG 1 cut(s) 242
XapI RAATTY 2 cut(s) 336, 361
XmaJI CCTAGG 1 cut(s) 178
XspI CTAG 2 cut(s) 179, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.