pycom10g12850

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
16326998 .. 16327608
611 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
ATGGGGACCCACCCCTTATCCCCCCACTTTTCTTCCCTCTTCCCGTTTTCCCCCACCATCCCTCCTCTCTTTCTCTCCCCTATCTCCCTTTCACGCTGCAACAACAGCCTCTTCGGCTCTGCCATTGTCCGCAAGCTCCAAGCCCTAAACTTCACAAACCTCGTCCTCTGTACCCACGTGCCTCGACGAGCTCGCCCTCTGCACCCACAGCTCCCCTTTAGTGATTTCTCCGCCTGCAGCAACGACATATCAGGAGAATTAAAATGGCTCGCAAGCTTGCCGTCGCCGGAAAACGCGCTGACCTCTGAATTATCTGAGGCTGCACCAGAACTGGAACCATGCCAGGGGTCTTTACATATGGAGAACTTCTCAACCGAGATCGATCGAGAGCATTTCGGTGGAAAATCTTCAGCCGACGATCAAGATCTGCGTCGACGGCCTCCAATCACCGTCGCTTGTCGTGAAGCAATATGCAGCGGACAAGCTCAAGCTTTTAACGAAGAATCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

171

Amino Acids

18.59

Weight (kDa)

5.63

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 433
AccII CGCG 1 cut(s) 296
AciI CCGC 3 cut(s) 130, 231, 477
AcuI CTGAAG 1 cut(s) 393
AcvI CACGTG 1 cut(s) 178
AfaI GTAC 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 344
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 6 cut(s) 136, 191, 211, 276, 485, 491
AluI AGCT 6 cut(s) 136, 191, 211, 276, 485, 491
Alw21I GWGCWC 1 cut(s) 193
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 4 cut(s) 96, 237, 320, 474
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 1 cut(s) 439
AvaII GGWCC 1 cut(s) 6
BanII GRGCYC 1 cut(s) 193
BbrPI CACGTG 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 4 cut(s) 83, 249, 307, 486
BccI CCATC 1 cut(s) 65
BceAI ACGGC 2 cut(s) 265, 452
BciT130I CCWGG 1 cut(s) 344
BfmI CTRYAG 1 cut(s) 235
BglI GCCNNNNNGGC 1 cut(s) 114
BglII AGATCT 1 cut(s) 424
BisI GCNGC 4 cut(s) 97, 238, 321, 475
BlsI GCNGC 4 cut(s) 98, 239, 322, 476
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 3 cut(s) 7, 8, 336
BmrFI CCNGG 1 cut(s) 344
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpuEI CTTGAG 1 cut(s) 471
Bsa29I ATCGAT 1 cut(s) 381
BsaAI YACGTR 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 1 cut(s) 344
Bse1I ACTGG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 423
BseBI CCWGG 1 cut(s) 344
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 1 cut(s) 57
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 344
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 54
BseXI GCAGC 4 cut(s) 83, 249, 307, 486
BsgI GTGCAG 2 cut(s) 185, 306
Bsh1236I CGCG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 385
BshFI GGCC 1 cut(s) 439
BshVI ATCGAT 1 cut(s) 381
BsiEI CGRYCG 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 4 cut(s) 378, 382, 418, 424
BspACI CCGC 3 cut(s) 130, 231, 477
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 1 cut(s) 307
BspDI ATCGAT 1 cut(s) 381
BspFNI CGCG 1 cut(s) 296
BspLI GGNNCC 3 cut(s) 7, 8, 336
BspMAI CTGCAG 1 cut(s) 239
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 4 cut(s) 378, 382, 418, 424
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 2 cut(s) 44, 116
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 6 cut(s) 134, 193, 235, 270, 274, 278
BstDEI CTNAG 1 cut(s) 315
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 4 cut(s) 381, 385, 421, 427
BstMBI GATC 4 cut(s) 378, 382, 418, 424
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 4 cut(s) 105, 114, 208, 436
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 235
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 4 cut(s) 83, 249, 307, 486
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
Bsu15I ATCGAT 1 cut(s) 381
BsuRI GGCC 1 cut(s) 439
BsuTUI ATCGAT 1 cut(s) 381
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 6 cut(s) 134, 193, 235, 270, 274, 278
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 6
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 1 cut(s) 419
Csp6I GTAC 1 cut(s) 171
CviAII CATG 1 cut(s) 339
CviQI GTAC 1 cut(s) 171
DdeI CTNAG 1 cut(s) 315
DpnI GATC 4 cut(s) 380, 384, 420, 426
DpnII GATC 4 cut(s) 378, 382, 418, 424
Eam1104I CTCTTC 2 cut(s) 44, 116
EarI CTCTTC 2 cut(s) 44, 116
EciI GGCGGA 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 393
Eco72I CACGTG 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 193
FaeI CATG 1 cut(s) 342
FaiI YATR 5 cut(s) 248, 340, 357, 359, 472
FaqI GGGAC 1 cut(s) 19
FatI CATG 1 cut(s) 338
FauNDI CATATG 1 cut(s) 357
FblI GTMKAC 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 97, 238, 321, 475
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 4 cut(s) 97, 238, 321, 475
GlaI GCGC 1 cut(s) 297
GluI GCNGC 4 cut(s) 97, 238, 321, 475
HaeIII GGCC 1 cut(s) 439
HapII CCGG 1 cut(s) 287
HgaI GACGC 1 cut(s) 419
HhaI GCGC 1 cut(s) 298
Hin1II CATG 1 cut(s) 342
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 434
HindII GTYRAC 1 cut(s) 434
HindIII AAGCTT 2 cut(s) 274, 489
HinfI GANTC 1 cut(s) 503
HpaII CCGG 1 cut(s) 287
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 2 cut(s) 307, 316
Hpy188III TCNNGA 4 cut(s) 252, 386, 422, 461
Hpy8I GTNNAC 1 cut(s) 434
Hpy99I CGWCG 6 cut(s) 189, 286, 419, 435, 438, 455
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 99, 202, 237, 323, 474
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 114, 208, 436
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 1 cut(s) 342
HspAI GCGC 1 cut(s) 296
KflI GGGWCCC 1 cut(s) 6
Kzo9I GATC 4 cut(s) 378, 382, 418, 424
LmnI GCTCC 2 cut(s) 141, 216
LpnPI CCDG 8 cut(s) 237, 247, 300, 317, 329, 339, 356, 492
Lsp1109I GCAGC 4 cut(s) 83, 249, 307, 486
MaeII ACGT 1 cut(s) 177
MalI GATC 4 cut(s) 380, 384, 420, 426
MboI GATC 4 cut(s) 378, 382, 418, 424
MboII GAAGA 5 cut(s) 24, 31, 103, 399, 512
MflI RGATCY 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 2 cut(s) 257, 308
MroXI GAANNNNTTC 1 cut(s) 406
MseI TTAA 2 cut(s) 260, 495
MslI CAYNNNNRTG 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 477
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 4 cut(s) 105, 114, 208, 436
NdeI CATATG 1 cut(s) 357
NdeII GATC 4 cut(s) 378, 382, 418, 424
NlaIII CATG 1 cut(s) 342
NlaIV GGNNCC 3 cut(s) 7, 8, 336
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 503
PkrI GCNGC 4 cut(s) 98, 239, 322, 476
Ple19I CGATCG 1 cut(s) 385
PmaCI CACGTG 1 cut(s) 178
PmlI CACGTG 1 cut(s) 178
Ppu21I YACGTR 1 cut(s) 178
PpuMI RGGWCCY 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 342
PspCI CACGTG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 3 cut(s) 7, 8, 336
PspPI GGNCC 1 cut(s) 6
PspPPI RGGWCCY 1 cut(s) 6
PstI CTGCAG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 424
PvuI CGATCG 1 cut(s) 385
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
RseI CAYNNNNRTG 1 cut(s) 396
SacI GAGCTC 1 cut(s) 193
SalI GTCGAC 1 cut(s) 432
SaqAI TTAA 2 cut(s) 260, 495
SatI GCNGC 4 cut(s) 97, 238, 321, 475
Sau3AI GATC 4 cut(s) 378, 382, 418, 424
Sau96I GGNCC 1 cut(s) 6
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 235
SgrDI CGTCGACG 1 cut(s) 432
SinI GGWCC 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 486
SmoI CTYRAG 1 cut(s) 486
Sse9I AATT 2 cut(s) 257, 308
SsiI CCGC 3 cut(s) 130, 231, 477
SstI GAGCTC 1 cut(s) 193
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 1 cut(s) 180
TaqI TCGA 4 cut(s) 184, 381, 385, 433
TasI AATT 2 cut(s) 257, 308
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 260, 495
Tru9I TTAA 2 cut(s) 260, 495
TseI GCWGC 4 cut(s) 96, 237, 320, 474
VpaK11BI GGWCC 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.