pycom10g13700

ER lumen protein retaining

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
17157753 .. 17158407
655 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGTACGTTGTAACTTTGGAAAAGGTGCAAGATTATTATTTTACCACAAAAACCGCAACGTACGTAATTTCATTTGAATTGCAGGTGGTGCCTGCTGCTATCCTCGGAGTGCTTGTCCACCCATATACACTATATAACCCCATCGGTCGAATCCTATGGGCATTTGGTGGATACATGGAAGCCGTTTCAGTACTGCCTCAACTCCGTTTAATGAAGAATGCAAAGATGATCGAACCATTTACCTCCCATTACGTATTTGCATTGGGCATTTCGAGGTTCTTGGCGTGCGCTCATTGGATTATTCGGATCTACGAGACTAGAGGAAGATATATATTTTTAGTCGGGAGTGGATATTTCTGGATTTTGGCAGCTTTCCTTTCTGAGATGGTTCAAACATTCATCTTAGCTGACTTTTGTTACTACTACATAAAGAGGTAA

Protein Analysis

146

Amino Acids

16.92

Weight (kDa)

9.06

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 2 - 102 3e-22 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 88
AciI CCGC 1 cut(s) 54
AclWI GGATC 1 cut(s) 314
AfaI GTAC 3 cut(s) 5, 62, 192
AgsI TTSAA 2 cut(s) 77, 392
AluBI AGCT 2 cut(s) 371, 407
AluI AGCT 2 cut(s) 371, 407
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 1 cut(s) 314
ApeKI GCWGC 2 cut(s) 95, 368
AspLEI GCGC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 82, 380
BccI CCATC 2 cut(s) 149, 379
BceAI ACGGC 1 cut(s) 167
BciVI GTATCC 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 164
BisI GCNGC 2 cut(s) 96, 369
BlsI GCNGC 2 cut(s) 97, 370
BmcAI AGTACT 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 90
BsaAI YACGTR 2 cut(s) 64, 253
BsaJI CCNNGG 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
BseDI CCNNGG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 372
BseXI GCAGC 2 cut(s) 82, 380
Bsh1285I CGRYCG 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 88
BsiEI CGRYCG 1 cut(s) 148
BsiWI CGTACG 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 223
Bsp143I GATC 2 cut(s) 228, 306
BspACI CCGC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 90
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 2 cut(s) 228, 306
BstAPI GCANNNNNTGC 1 cut(s) 88
BstBAI YACGTR 2 cut(s) 64, 253
BstC8I GCNNGC 2 cut(s) 93, 286
BstDEI CTNAG 2 cut(s) 381, 403
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 2 cut(s) 231, 309
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 2 cut(s) 228, 306
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstSNI TACGTA 2 cut(s) 64, 253
BstV1I GCAGC 2 cut(s) 82, 380
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BsuI GTATCC 1 cut(s) 164
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 93, 286
CfoI GCGC 1 cut(s) 290
Csp6I GTAC 3 cut(s) 4, 61, 191
CviAII CATG 1 cut(s) 175
CviJI RGCY 3 cut(s) 182, 371, 407
CviKI_1 RGCY 3 cut(s) 182, 371, 407
CviQI GTAC 3 cut(s) 4, 61, 191
DdeI CTNAG 2 cut(s) 381, 403
DpnI GATC 2 cut(s) 230, 308
DpnII GATC 2 cut(s) 228, 306
Eco105I TACGTA 2 cut(s) 64, 253
FaeI CATG 1 cut(s) 178
FaiI YATR 9 cut(s) 124, 126, 133, 135, 157, 176, 330, 332, 428
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 96, 369
Fsp4HI GCNGC 2 cut(s) 96, 369
FspBI CTAG 1 cut(s) 318
GlaI GCGC 1 cut(s) 289
GluI GCNGC 2 cut(s) 96, 369
HhaI GCGC 1 cut(s) 290
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HinfI GANTC 1 cut(s) 150
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 3 cut(s) 107, 306, 382
Hpy188III TCNNGA 2 cut(s) 343, 358
Hpy8I GTNNAC 1 cut(s) 118
HpyCH4IV ACGT 4 cut(s) 6, 59, 63, 252
HpyCH4V TGCA 4 cut(s) 28, 82, 221, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 2 cut(s) 381, 403
HpySE526I ACGT 4 cut(s) 6, 59, 63, 252
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 1 cut(s) 288
Kzo9I GATC 2 cut(s) 228, 306
LpnPI CCDG 3 cut(s) 68, 105, 343
Lsp1109I GCAGC 2 cut(s) 82, 380
MaeI CTAG 1 cut(s) 318
MaeII ACGT 4 cut(s) 6, 59, 63, 252
MaeIII GTNAC 2 cut(s) 10, 416
MalI GATC 2 cut(s) 230, 308
MboI GATC 2 cut(s) 228, 306
MboII GAAGA 2 cut(s) 226, 336
MflI RGATCY 1 cut(s) 306
MluCI AATT 2 cut(s) 66, 77
MnlI CCTC 6 cut(s) 113, 207, 253, 267, 314, 426
MseI TTAA 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 88
NdeII GATC 2 cut(s) 228, 306
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 90
PaqCI CACCTGC 1 cut(s) 73
PctI GAATGC 1 cut(s) 223
PfeI GAWTC 1 cut(s) 150
Pfl23II CGTACG 1 cut(s) 60
PkrI GCNGC 2 cut(s) 97, 370
Ppu21I YACGTR 2 cut(s) 64, 253
PspLI CGTACG 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 90
PsuI RGATCY 1 cut(s) 306
RsaI GTAC 3 cut(s) 5, 62, 192
RsaNI GTAC 3 cut(s) 4, 61, 191
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 2 cut(s) 96, 369
Sau3AI GATC 2 cut(s) 228, 306
ScaI AGTACT 1 cut(s) 192
SnaBI TACGTA 2 cut(s) 64, 253
Sse9I AATT 2 cut(s) 66, 77
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 318
TaiI ACGT 4 cut(s) 9, 62, 66, 255
TaqI TCGA 3 cut(s) 148, 231, 272
TaqII GACCGA 1 cut(s) 134
TasI AATT 2 cut(s) 66, 77
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TseI GCWGC 2 cut(s) 95, 368
TspDTI ATGAA 3 cut(s) 60, 227, 388
TspGWI ACGGA 1 cut(s) 194
XspI CTAG 1 cut(s) 318
ZrmI AGTACT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.