pycom10g14580

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
17956229 .. 17959643
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGAGAGCTTGCTCTAAACCAACAATGGTGGTCTCTTTGAACTTGTTTTTGTTACTTTCGACAATGGCGGTTCTTCTAGCTCTGGTTGCAAGCACAAATGCCTCTGCAGAGACTGACAAGAATGTAAATTCTAGGTTTGTGGGACCAAAGGAGTCATTGAGCTCCAAGAACTCATCAATGGCAGAAAGGTCTGATGAAGCTTTGGATGAGCATGCAGTAGATAATCCGGATGAAATAGCCTCCATGGTTGACATGACCATTCGTAATAGCACAGAGAGAAGAAATTTGGGATTCTTTTCATGTGCGACGGGGAATCCAATTGATGATTGTTGGAGATGTGACCCACAGTGGCAGCTCCACCGCAAGCGCCTTGCAAACTGCGGCATAGGGTTTGGGCGCAACGCCGTGGGTGGCCGCGACGGGAGATACTACGTCGTAAACGACCCTGGCAATGATGACCCCGTCAACCCTAGACCCGGAACCCTCCGCCACGCCGTCATCCAGGACAGGCCGTTGTGGATTGTGTTCAAGCGTGACATGGTGATCGATCAAAGTAGCAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

189

Amino Acids

20.83

Weight (kDa)

6.82

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 417
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 5 cut(s) 68, 361, 381, 415, 487
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 127, 283
AfiI CCNNNNNNNGG 1 cut(s) 476
AgsI TTSAA 2 cut(s) 40, 529
AjnI CCWGG 2 cut(s) 445, 501
AluBI AGCT 6 cut(s) 8, 80, 162, 200, 355, 564
AluI AGCT 6 cut(s) 8, 80, 162, 200, 355, 564
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 37, 104
AlwNI CAGNNNCTG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 2 cut(s) 412, 509
ApeKI GCWGC 1 cut(s) 352
ApoI RAATTY 2 cut(s) 127, 283
AspLEI GCGC 2 cut(s) 369, 399
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 1 cut(s) 553
AvaII GGWCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 364
BceAI ACGGC 3 cut(s) 389, 479, 496
BciT130I CCWGG 2 cut(s) 447, 503
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 2 cut(s) 37, 104
BfaI CTAG 3 cut(s) 77, 132, 471
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 1 cut(s) 370
BisI GCNGC 3 cut(s) 353, 382, 415
BlsI GCNGC 3 cut(s) 354, 383, 416
Bme1390I CCNGG 3 cut(s) 447, 477, 503
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 144, 481
BmrFI CCNGG 3 cut(s) 447, 477, 503
BpuMI CCSGG 1 cut(s) 477
Bsa29I ATCGAT 1 cut(s) 546
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 243, 405, 445
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 457
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 2 cut(s) 447, 503
BseCI ATCGAT 1 cut(s) 546
BseDI CCNNGG 3 cut(s) 243, 405, 445
BseGI GGATG 3 cut(s) 211, 235, 498
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMI GCAATG 1 cut(s) 457
BseXI GCAGC 1 cut(s) 364
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 2 cut(s) 414, 511
BshVI ATCGAT 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 2 cut(s) 227, 477
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 476
BsmAI GTCTC 2 cut(s) 37, 104
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 2 cut(s) 414, 511
Bso31I GGTCTC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 164
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 2 cut(s) 543, 547
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 5 cut(s) 68, 361, 381, 415, 487
BspANI GGCC 2 cut(s) 414, 511
BspDI ATCGAT 1 cut(s) 546
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 144, 481
BspMAI CTGCAG 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 457
BssECI CCNNGG 3 cut(s) 243, 405, 445
BssMI GATC 2 cut(s) 543, 547
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 447, 503
Bst4CI ACNGT 1 cut(s) 348
BstC8I GCNNGC 4 cut(s) 10, 91, 213, 365
BstDSI CCRYGG 2 cut(s) 243, 405
BstF5I GGATG 3 cut(s) 211, 235, 498
BstFNI CGCG 1 cut(s) 417
BstH2I RGCGCY 1 cut(s) 370
BstHHI GCGC 2 cut(s) 369, 399
BstKTI GATC 2 cut(s) 546, 550
BstMAI GTCTC 2 cut(s) 37, 104
BstMBI GATC 2 cut(s) 543, 547
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstNI CCWGG 2 cut(s) 447, 503
BstNSI RCATGY 1 cut(s) 215
BstSCI CCNGG 3 cut(s) 445, 475, 501
BstSFI CTRYAG 1 cut(s) 105
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 364
Bsu15I ATCGAT 1 cut(s) 546
BsuRI GGCC 2 cut(s) 414, 511
BsuTUI ATCGAT 1 cut(s) 546
BtgI CCRYGG 2 cut(s) 243, 405
BtsCI GGATG 3 cut(s) 211, 235, 498
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 4 cut(s) 10, 91, 213, 365
CaiI CAGNNNCTG 1 cut(s) 113
CfoI GCGC 2 cut(s) 369, 399
Cfr13I GGNCC 1 cut(s) 143
ClaI ATCGAT 1 cut(s) 546
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 5 cut(s) 212, 244, 253, 300, 538
CviJI RGCY 9 cut(s) 8, 80, 162, 200, 239, 355, 414, 511, 564
CviKI_1 RGCY 9 cut(s) 8, 80, 162, 200, 239, 355, 414, 511, 564
DpnI GATC 2 cut(s) 545, 549
DpnII GATC 2 cut(s) 543, 547
EaeI YGGCCR 1 cut(s) 412
EciI GGCGGA 1 cut(s) 476
Ecl136II GAGCTC 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 143
Eco53kI GAGCTC 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 445, 501
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 5 cut(s) 215, 247, 256, 303, 541
FaiI YATR 6 cut(s) 213, 245, 254, 301, 386, 539
FaqI GGGAC 1 cut(s) 156
FatI CATG 5 cut(s) 211, 243, 252, 299, 537
Fnu4HI GCNGC 3 cut(s) 353, 382, 415
FokI GGATG 3 cut(s) 218, 242, 485
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 353, 382, 415
FspBI CTAG 3 cut(s) 77, 132, 471
GlaI GCGC 2 cut(s) 368, 398
GluI GCNGC 3 cut(s) 353, 382, 415
HaeII RGCGCY 1 cut(s) 370
HaeIII GGCC 2 cut(s) 414, 511
HapII CCGG 2 cut(s) 227, 477
HhaI GCGC 2 cut(s) 369, 399
Hin1II CATG 5 cut(s) 215, 247, 256, 303, 541
Hin6I GCGC 2 cut(s) 367, 397
HinP1I GCGC 2 cut(s) 367, 397
HincII GTYRAC 2 cut(s) 250, 466
HindII GTYRAC 2 cut(s) 250, 466
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 3 cut(s) 152, 291, 313
HpaII CCGG 2 cut(s) 227, 477
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 3 cut(s) 250, 439, 466
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 3 cut(s) 250, 439, 466
Hpy99I CGWCG 3 cut(s) 310, 422, 437
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 4 cut(s) 89, 107, 215, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 5 cut(s) 215, 247, 256, 303, 541
HspAI GCGC 2 cut(s) 367, 397
Kpn2I TCCGGA 1 cut(s) 226
Kzo9I GATC 2 cut(s) 543, 547
LmnI GCTCC 2 cut(s) 167, 360
LpnPI CCDG 8 cut(s) 68, 240, 432, 459, 488, 490, 493, 515
Lsp1109I GCAGC 1 cut(s) 364
MaeI CTAG 3 cut(s) 77, 132, 471
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 3 cut(s) 51, 338, 533
MalI GATC 2 cut(s) 545, 549
MboI GATC 2 cut(s) 543, 547
MboII GAAGA 2 cut(s) 65, 291
MfeI CAATTG 1 cut(s) 318
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 3 cut(s) 127, 283, 318
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 3 cut(s) 112, 250, 494
MroI TCCGGA 1 cut(s) 226
MspI CCGG 2 cut(s) 227, 477
MspR9I CCNGG 3 cut(s) 447, 477, 503
MunI CAATTG 1 cut(s) 318
MvaI CCWGG 2 cut(s) 447, 503
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 1 cut(s) 86
NciI CCSGG 1 cut(s) 477
NcoI CCATGG 1 cut(s) 243
NdeII GATC 2 cut(s) 543, 547
NlaIII CATG 5 cut(s) 215, 247, 256, 303, 541
NlaIV GGNNCC 2 cut(s) 144, 481
NmuCI GTSAC 2 cut(s) 338, 533
NspI RCATGY 1 cut(s) 215
PaeI GCATGC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PfeI GAWTC 2 cut(s) 291, 313
PflFI GACNNNGTC 1 cut(s) 461
PfoI TCCNGGA 1 cut(s) 501
PkrI GCNGC 3 cut(s) 354, 383, 416
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 164
Psp6I CCWGG 2 cut(s) 445, 501
PspGI CCWGG 2 cut(s) 445, 501
PspN4I GGNNCC 2 cut(s) 144, 481
PspPI GGNCC 1 cut(s) 143
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 113
PsyI GACNNNGTC 1 cut(s) 461
SacI GAGCTC 1 cut(s) 164
SatI GCNGC 3 cut(s) 353, 382, 415
Sau3AI GATC 2 cut(s) 543, 547
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 3 cut(s) 447, 477, 503
SduI GDGCHC 1 cut(s) 164
SetI ASST 9 cut(s) 10, 82, 137, 164, 191, 202, 357, 435, 566
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 143
SphI GCATGC 1 cut(s) 215
Sse9I AATT 3 cut(s) 127, 283, 318
SsiI CCGC 5 cut(s) 68, 361, 381, 415, 487
SspMI CTAG 3 cut(s) 77, 132, 471
SstI GAGCTC 1 cut(s) 164
StyD4I CCNGG 3 cut(s) 445, 475, 501
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 348
TaiI ACGT 1 cut(s) 435
TaqI TCGA 2 cut(s) 59, 546
TasI AATT 3 cut(s) 127, 283, 318
TauI GCSGC 2 cut(s) 384, 417
TfiI GAWTC 2 cut(s) 291, 313
TscAI CASTG 1 cut(s) 353
TseFI GTSAC 2 cut(s) 338, 533
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 2 cut(s) 338, 533
TspDTI ATGAA 3 cut(s) 210, 246, 288
TspRI CASTG 1 cut(s) 353
Tth111I GACNNNGTC 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 2 cut(s) 127, 283
XceI RCATGY 1 cut(s) 215
XspI CTAG 3 cut(s) 77, 132, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.