pycom10g14590

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
17959823 .. 17960798
976 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGGCGGACGGGGATGGCATTTCCATTTTTGGATCTAGCCACATTTGGATTGACCACAATTCCCTTTCTAATTGTGCTGATGGGCTCATTGATGCTATTATGGGCTCTACTGCAATTACCATTTCCAACAACTACTTCACCCATCATAATGAGGTTATGTTACTGGGTCACAGTGACTCCTATGTAAGGGACAAGCTTATGCAAGTGACAATTGCTTACAATCATTTTGGTGAGGGACTTATTCAAAGGATGCCAAGGTGTAGGCACGGGTATTTCCATGTGGTGAACAATGACTACACACATTGGGAAATGTATGCCATTGGTGGAAGTGCTGAACCCACAATTAACAGCCAGGGCAACAGATATCTGGCCCCTAACAATGCTTTTGCAAAGGAGGTCACACATAGAGTTGATCCAAATGACTGGACGCACTGGAACTGGATCGATCAGAAGGAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

154

Amino Acids

17.41

Weight (kDa)

5.66

Isoelectric Point (pI)

19.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 3 - 143 2.3e-18 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 40, 407, 449
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 1 cut(s) 245
AjnI CCWGG 1 cut(s) 351
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 3 cut(s) 40, 407, 449
AoxI GGCC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 3 cut(s) 130, 242, 295
BanII GRGCYC 2 cut(s) 87, 107
BccI CCATC 3 cut(s) 8, 74, 150
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 1 cut(s) 353
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 2 cut(s) 82, 240
BmuI ACTGGG 1 cut(s) 173
Bsa29I ATCGAT 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 254, 352
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 4 cut(s) 168, 428, 437, 443
BseBI CCWGG 1 cut(s) 353
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 254, 352
BseGI GGATG 2 cut(s) 19, 255
BseLI CCNNNNNNNGG 1 cut(s) 186
BseNI ACTGG 4 cut(s) 168, 428, 437, 443
BshFI GGCC 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 444
BslFI GGGAC 2 cut(s) 203, 249
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 2 cut(s) 203, 249
BsnI GGCC 1 cut(s) 371
Bso31I GGTCTC 1 cut(s) 449
Bsp1286I GDGCHC 2 cut(s) 87, 107
Bsp143I GATC 4 cut(s) 32, 412, 441, 445
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 371
BspDI ATCGAT 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 372
BspPI GGATC 3 cut(s) 40, 407, 449
BspTNI GGTCTC 1 cut(s) 449
BsrI ACTGG 4 cut(s) 168, 428, 437, 443
BssECI CCNNGG 2 cut(s) 254, 352
BssMI GATC 4 cut(s) 32, 412, 441, 445
BssT1I CCWWGG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 173
BstENI CCTNNNNNAGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 19, 255
BstKTI GATC 4 cut(s) 35, 415, 444, 448
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 4 cut(s) 32, 412, 441, 445
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 32
BstXI CCANNNNNNTGG 1 cut(s) 423
BstYI RGATCY 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 1 cut(s) 371
BsuTUI ATCGAT 1 cut(s) 444
BtsCI GGATG 2 cut(s) 19, 255
BtsIMutI CAGTG 2 cut(s) 178, 430
Cfr13I GGNCC 1 cut(s) 370
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 1 cut(s) 436
CviAII CATG 1 cut(s) 278
CviJI RGCY 6 cut(s) 39, 85, 105, 196, 351, 371
CviKI_1 RGCY 6 cut(s) 39, 85, 105, 196, 351, 371
DpnI GATC 4 cut(s) 34, 414, 443, 447
DpnII GATC 4 cut(s) 32, 412, 441, 445
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 254
Eco24I GRGCYC 2 cut(s) 87, 107
Eco31I GGTCTC 1 cut(s) 449
Eco32I GATATC 1 cut(s) 365
EcoNI CCTNNNNNAGG 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 351
EcoRV GATATC 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 254
EcoT38I GRGCYC 2 cut(s) 87, 107
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 1 cut(s) 281
FaiI YATR 8 cut(s) 101, 147, 158, 183, 200, 279, 315, 405
FaqI GGGAC 2 cut(s) 203, 249
FatI CATG 1 cut(s) 277
FokI GGATG 2 cut(s) 26, 262
FriOI GRGCYC 2 cut(s) 87, 107
FspBI CTAG 1 cut(s) 36
HaeIII GGCC 1 cut(s) 371
HgaI GACGC 1 cut(s) 436
Hin1II CATG 1 cut(s) 281
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 1 cut(s) 176
HphI GGTGA 3 cut(s) 130, 242, 295
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 1 cut(s) 450
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 1 cut(s) 445
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 3 cut(s) 113, 202, 389
Hsp92II CATG 1 cut(s) 281
Kzo9I GATC 4 cut(s) 32, 412, 441, 445
LpnPI CCDG 7 cut(s) 149, 338, 353, 365, 409, 418, 424
LweI GCATC 2 cut(s) 82, 240
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 5 cut(s) 159, 167, 173, 205, 397
MalI GATC 4 cut(s) 34, 414, 443, 447
MboI GATC 4 cut(s) 32, 412, 441, 445
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 87, 107
MluCI AATT 5 cut(s) 58, 70, 114, 210, 342
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 3 cut(s) 145, 226, 388
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 2 cut(s) 147, 228
MspR9I CCNGG 1 cut(s) 353
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 1 cut(s) 353
NdeII GATC 4 cut(s) 32, 412, 441, 445
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 4 cut(s) 167, 173, 205, 397
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 370
PsrI GAACNNNNNNTAC 2 cut(s) 278, 310
PsuI RGATCY 1 cut(s) 32
RseI CAYNNNNRTG 2 cut(s) 147, 228
SaqAI TTAA 1 cut(s) 345
Sau3AI GATC 4 cut(s) 32, 412, 441, 445
Sau96I GGNCC 1 cut(s) 370
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 2 cut(s) 87, 107
SetI ASST 5 cut(s) 156, 198, 260, 399, 461
SfaNI GCATC 2 cut(s) 82, 240
SmiMI CAYNNNNRTG 2 cut(s) 147, 228
Sse9I AATT 5 cut(s) 58, 70, 114, 210, 342
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 351
StyI CCWWGG 1 cut(s) 254
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 1 cut(s) 444
TasI AATT 5 cut(s) 58, 70, 114, 210, 342
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 2 cut(s) 178, 437
TseFI GTSAC 4 cut(s) 167, 173, 205, 397
Tsp45I GTSAC 4 cut(s) 167, 173, 205, 397
TspRI CASTG 2 cut(s) 178, 437
XagI CCTNNNNNAGG 1 cut(s) 184
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.