pycom10g16750

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029382
Physical Location & Seq
Forward (+)
19893067 .. 19893519
453 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
ATGGCTGCCGCGAAGCGCATTTTGAAGGAGCTCAAAGATTTACAGAAGGATCCTCCTAATCATATCAGTGCAGGTCCTCAATCCTCCGGAAACCTATTCAAATGGCAAGCTACAATTATGGGACCCCCTGCTAGCCCATATGAAGGAGGTGTATTCTACCTTGAGATTAACTTCCCCGCTGATTATCCATTTAAACCGCCAAGGGTTCAGTTTAAGACTAAGGTCTACCACCCAAATATCAGTAGCGATGGAACCATCTGTCTCGATACCCTAAAAAAAAATTGGAGCGCTGCTCTTAGTATTTCTAAAGTGTTGATCTCAATATGCTCATTGCTAACTGATCCAAATCCCGACGACCCTCTCGAAAAGGAGATTGCTCGGCTCTATAAAAATAATCGAGGCAAGTACGAAGAAAATGCACGTATCTGGACTCAGAAGCATGCAATGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

151

Amino Acids

16.84

Weight (kDa)

9.19

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 6 - 142 8.6e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029382)

Species Orthologous Gene IDs
pyrus_communis pycom10g16740 pycom10g16750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 86
AciI CCGC 3 cut(s) 9, 177, 197
AclWI GGATC 3 cut(s) 44, 57, 335
AfaI GTAC 1 cut(s) 407
AfeI AGCGCT 1 cut(s) 289
AfiI CCNNNNNNNGG 1 cut(s) 143
AgsI TTSAA 2 cut(s) 25, 100
AluBI AGCT 2 cut(s) 31, 110
AluI AGCT 2 cut(s) 31, 110
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 3 cut(s) 44, 57, 335
Aor13HI TCCGGA 1 cut(s) 86
Aor51HI AGCGCT 1 cut(s) 289
ApeKI GCWGC 2 cut(s) 5, 290
AspLEI GCGC 2 cut(s) 18, 290
AspS9I GGNCC 2 cut(s) 74, 122
AsuNHI GCTAGC 1 cut(s) 131
AvaII GGWCC 2 cut(s) 74, 122
BamHI GGATCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 242, 263
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 62
BisI GCNGC 3 cut(s) 6, 9, 291
BlsI GCNGC 3 cut(s) 7, 10, 292
Bme18I GGWCC 2 cut(s) 74, 122
BmgT120I GGNCC 2 cut(s) 74, 122
BmiI GGNNCC 4 cut(s) 51, 123, 124, 253
BmtI GCTAGC 1 cut(s) 135
BoxI GACNNNNGTC 1 cut(s) 221
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpuEI CTTGAG 1 cut(s) 182
BsaAI YACGTR 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse3DI GCAATG 2 cut(s) 329, 450
Bse8I GATNNNNATC 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 200
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMI GCAATG 2 cut(s) 329, 450
BseMII CTCAG 1 cut(s) 446
BseXI GCAGC 1 cut(s) 277
BsgI GTGCAG 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 33
Bsp13I TCCGGA 1 cut(s) 86
Bsp143I GATC 3 cut(s) 49, 315, 340
BspACI CCGC 3 cut(s) 9, 177, 197
BspCNI CTCAG 1 cut(s) 445
BspEI TCCGGA 1 cut(s) 86
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 4 cut(s) 51, 123, 124, 253
BspMI ACCTGC 1 cut(s) 62
BspOI GCTAGC 1 cut(s) 135
BspPI GGATC 3 cut(s) 44, 57, 335
BsrDI GCAATG 2 cut(s) 329, 450
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 3 cut(s) 49, 315, 340
BssT1I CCWWGG 1 cut(s) 200
BstBAI YACGTR 1 cut(s) 422
BstC8I GCNNGC 3 cut(s) 108, 133, 441
BstDEI CTNAG 3 cut(s) 219, 296, 432
BstFNI CGCG 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 18, 290
BstKTI GATC 3 cut(s) 52, 318, 343
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 3 cut(s) 49, 315, 340
BstNSI RCATGY 1 cut(s) 443
BstPAI GACNNNNGTC 1 cut(s) 221
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BtgZI GCGATG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 73
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 3 cut(s) 108, 133, 441
CfoI GCGC 2 cut(s) 18, 290
Cfr13I GGNCC 2 cut(s) 74, 122
Csp6I GTAC 1 cut(s) 406
CviAII CATG 1 cut(s) 440
CviJI RGCY 5 cut(s) 5, 31, 110, 135, 382
CviKI_1 RGCY 5 cut(s) 5, 31, 110, 135, 382
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 3 cut(s) 219, 296, 432
DpnI GATC 3 cut(s) 51, 317, 342
DpnII GATC 3 cut(s) 49, 315, 340
DraI TTTAAA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 2 cut(s) 74, 122
Eco47III AGCGCT 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 31
EcoO109I RGGNCCY 2 cut(s) 74, 122
EcoT14I CCWWGG 1 cut(s) 200
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 1 cut(s) 443
FaiI YATR 7 cut(s) 63, 119, 139, 141, 325, 387, 441
FaqI GGGAC 1 cut(s) 135
FatI CATG 1 cut(s) 439
FauI CCCGC 1 cut(s) 184
FauNDI CATATG 1 cut(s) 139
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 6, 9, 291
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 6, 9, 291
FspBI CTAG 1 cut(s) 132
GlaI GCGC 2 cut(s) 17, 289
GluI GCNGC 3 cut(s) 6, 9, 291
HaeII RGCGCY 1 cut(s) 291
HapII CCGG 1 cut(s) 87
HhaI GCGC 2 cut(s) 18, 290
Hin1II CATG 1 cut(s) 443
Hin6I GCGC 2 cut(s) 16, 288
HinP1I GCGC 2 cut(s) 16, 288
HinfI GANTC 1 cut(s) 430
HpaII CCGG 1 cut(s) 87
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 435
Hpy188III TCNNGA 5 cut(s) 87, 263, 350, 362, 427
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 3 cut(s) 19, 40, 137
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 3 cut(s) 71, 419, 443
HpyF3I CTNAG 3 cut(s) 219, 296, 432
HpySE526I ACGT 1 cut(s) 421
Hsp92II CATG 1 cut(s) 443
HspAI GCGC 2 cut(s) 16, 288
KflI GGGWCCC 1 cut(s) 122
Kpn2I TCCGGA 1 cut(s) 86
Kzo9I GATC 3 cut(s) 49, 315, 340
LmnI GCTCC 2 cut(s) 28, 285
LpnPI CCDG 4 cut(s) 57, 100, 141, 412
Lsp1109I GCAGC 1 cut(s) 277
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 421
MalI GATC 3 cut(s) 51, 317, 342
MboI GATC 3 cut(s) 49, 315, 340
MboII GAAGA 1 cut(s) 422
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 2 cut(s) 114, 280
MlyI GAGTC 1 cut(s) 424
MnlI CCTC 6 cut(s) 63, 87, 94, 140, 369, 392
MroI TCCGGA 1 cut(s) 86
MseI TTAA 3 cut(s) 168, 192, 213
MslI CAYNNNNRTG 1 cut(s) 66
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 11
NdeI CATATG 1 cut(s) 139
NdeII GATC 3 cut(s) 49, 315, 340
NheI GCTAGC 1 cut(s) 131
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 4 cut(s) 51, 123, 124, 253
NmeAIII GCCGAG 1 cut(s) 358
NspI RCATGY 1 cut(s) 443
PaeI GCATGC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PkrI GCNGC 3 cut(s) 7, 10, 292
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Ppu21I YACGTR 1 cut(s) 422
PpuMI RGGWCCY 2 cut(s) 74, 122
PshAI GACNNNNGTC 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 33
Psp5II RGGWCCY 2 cut(s) 74, 122
PspN4I GGNNCC 4 cut(s) 51, 123, 124, 253
PspPI GGNCC 2 cut(s) 74, 122
PspPPI RGGWCCY 2 cut(s) 74, 122
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 66
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 3 cut(s) 168, 192, 213
SatI GCNGC 3 cut(s) 6, 9, 291
Sau3AI GATC 3 cut(s) 49, 315, 340
Sau96I GGNCC 2 cut(s) 74, 122
SchI GAGTC 1 cut(s) 424
SduI GDGCHC 1 cut(s) 33
SetI ASST 8 cut(s) 33, 76, 96, 112, 151, 162, 225, 424
SinI GGWCC 2 cut(s) 74, 122
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SphI GCATGC 1 cut(s) 443
Sse9I AATT 2 cut(s) 114, 280
SsiI CCGC 3 cut(s) 9, 177, 197
SspMI CTAG 1 cut(s) 132
SstI GAGCTC 1 cut(s) 33
StyI CCWWGG 1 cut(s) 200
TaiI ACGT 1 cut(s) 424
TaqI TCGA 3 cut(s) 264, 363, 397
TasI AATT 2 cut(s) 114, 280
TauI GCSGC 1 cut(s) 11
Tru1I TTAA 3 cut(s) 168, 192, 213
Tru9I TTAA 3 cut(s) 168, 192, 213
TscAI CASTG 1 cut(s) 73
TseI GCWGC 2 cut(s) 5, 290
TspDTI ATGAA 1 cut(s) 156
TspRI CASTG 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 74, 122
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.