pycom10g18280

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
21582732 .. 21584397
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
ATGACCCAATTGATCGATCACCTCCTTCACATTAAAGACATCCCATTTGAAACTTCCATAGCGCATTACCTTATACAGCAGTATTTGGCAGCCACGGGGTGCTTGAAGCAACAGAAGTGTAACAAAAACATGTATGCAAGTGGGAGCGTGAAGATGGTTTGCTGTGAAACGGAGGTTTCTGCAGGAAGAAATGTGAAGACATTGGGAACAAGAAGTGGGGAAAACGGGTGCTTTGTTCTTTGGCAGATGTTGCCTGGAATGTGGTCTTTGGAGTTGGTCGTTGGAGGCAACAAGGTGGTGGCTGGTAGTGATGGCAAGACGGTTTGGAGGCACACCCCTTGGCTGGGTACTCATGCAGCCAAAGGCCCCCAGCGCCCGTTGCGCCGCCTCATACAGGGCCTAGATCCAAAGAGCACAGCAAGCTTGTTCGCCAAGGCACAATGCTTGGGAGAGAAAAGAATCGGAGATGATGACTGCTTTGTACTAAAAGTCTCCGCCGATCGAGCGGCTGTGATGGAAAGAAGTGAGGGTCCTGCAGAGGTCGATCCGACATGCATTGTATGGATATTTTTGCCAAAAGAGTGGCCTCCTCATTTATTTAGAGGACTCACACCTCACCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

209

Amino Acids

23.01

Weight (kDa)

8.79

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 44 - 181 2e-57 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 506
AciI CCGC 3 cut(s) 385, 495, 506
AclWI GGATC 2 cut(s) 398, 539
AdeI CACNNNGTG 1 cut(s) 99
AfaI GTAC 2 cut(s) 349, 483
AfiI CCNNNNNNNGG 3 cut(s) 343, 344, 394
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 2 cut(s) 50, 106
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 1 cut(s) 423
AluI AGCT 1 cut(s) 423
Alw21I GWGCWC 1 cut(s) 416
Alw26I GTCTC 1 cut(s) 496
AlwI GGATC 2 cut(s) 398, 539
AoxI GGCC 3 cut(s) 364, 397, 584
ApeKI GCWGC 2 cut(s) 89, 356
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
AspLEI GCGC 3 cut(s) 64, 375, 384
AspS9I GGNCC 3 cut(s) 365, 397, 530
AsuHPI GGTGA 2 cut(s) 11, 608
AvaII GGWCC 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 416
BbvI GCAGC 2 cut(s) 101, 368
BccI CCATC 3 cut(s) 148, 305, 508
BciT130I CCWGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 496
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 2 cut(s) 180, 534
BfoI RGCGCY 1 cut(s) 376
BisI GCNGC 4 cut(s) 90, 357, 385, 507
BlsI GCNGC 4 cut(s) 91, 358, 386, 508
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 3 cut(s) 365, 397, 530
BmiI GGNNCC 2 cut(s) 367, 531
BmrFI CCNGG 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 15
BsaJI CCNNGG 3 cut(s) 93, 338, 432
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 344, 394
BseBI CCWGG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 3 cut(s) 93, 338, 432
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 3 cut(s) 343, 344, 394
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 2 cut(s) 101, 368
BseYI CCCAGC 2 cut(s) 343, 369
Bsh1285I CGRYCG 1 cut(s) 502
BshFI GGCC 3 cut(s) 366, 399, 586
BshVI ATCGAT 1 cut(s) 15
BsiEI CGRYCG 1 cut(s) 502
BsiHKAI GWGCWC 1 cut(s) 416
BslI CCNNNNNNNGG 3 cut(s) 343, 344, 394
BsmAI GTCTC 1 cut(s) 496
BsnI GGCC 3 cut(s) 366, 399, 586
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 5 cut(s) 12, 16, 403, 499, 544
BspACI CCGC 3 cut(s) 385, 495, 506
BspANI GGCC 3 cut(s) 366, 399, 586
BspDI ATCGAT 1 cut(s) 15
BspLI GGNNCC 2 cut(s) 367, 531
BspMAI CTGCAG 2 cut(s) 184, 538
BspPI GGATC 2 cut(s) 398, 539
BsrBI CCGCTC 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 93, 338, 432
BssMI GATC 5 cut(s) 12, 16, 403, 499, 544
BssT1I CCWWGG 2 cut(s) 338, 432
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 322
BstAPI GCANNNNNTGC 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 421
BstDSI CCRYGG 1 cut(s) 93
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 3 cut(s) 64, 375, 384
BstKTI GATC 5 cut(s) 15, 19, 406, 502, 547
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 5 cut(s) 12, 16, 403, 499, 544
BstMCI CGRYCG 1 cut(s) 502
BstMWI GCNNNNNNNGC 6 cut(s) 250, 372, 379, 381, 420, 503
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 2 cut(s) 133, 555
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 2 cut(s) 180, 534
BstV1I GCAGC 2 cut(s) 101, 368
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 403
BstXI CCANNNNNNTGG 1 cut(s) 582
BstYI RGATCY 1 cut(s) 403
Bsu15I ATCGAT 1 cut(s) 15
BsuRI GGCC 3 cut(s) 366, 399, 586
BsuTUI ATCGAT 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 421
CfoI GCGC 3 cut(s) 64, 375, 384
Cfr13I GGNCC 3 cut(s) 365, 397, 530
ClaI ATCGAT 1 cut(s) 15
Csp6I GTAC 2 cut(s) 348, 482
CviAII CATG 3 cut(s) 130, 353, 552
CviJI RGCY 9 cut(s) 92, 302, 343, 359, 366, 399, 423, 509, 586
CviKI_1 RGCY 9 cut(s) 92, 302, 343, 359, 366, 399, 423, 509, 586
CviQI GTAC 2 cut(s) 348, 482
DpnI GATC 5 cut(s) 14, 18, 405, 501, 546
DpnII GATC 5 cut(s) 12, 16, 403, 499, 544
DraIII CACNNNGTG 1 cut(s) 99
EciI GGCGGA 1 cut(s) 484
Eco130I CCWWGG 2 cut(s) 338, 432
Eco47I GGWCC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoO109I RGGNCCY 3 cut(s) 365, 397, 530
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 338, 432
EcoT22I ATGCAT 1 cut(s) 557
ErhI CCWWGG 2 cut(s) 338, 432
FaeI CATG 3 cut(s) 133, 356, 555
FaiI YATR 8 cut(s) 59, 74, 131, 135, 354, 392, 553, 562
FatI CATG 3 cut(s) 129, 352, 551
Fnu4HI GCNGC 4 cut(s) 90, 357, 385, 507
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 4 cut(s) 90, 357, 385, 507
FspBI CTAG 1 cut(s) 401
GlaI GCGC 3 cut(s) 63, 374, 383
GluI GCNGC 4 cut(s) 90, 357, 385, 507
GsaI CCCAGC 2 cut(s) 347, 373
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 3 cut(s) 366, 399, 586
HhaI GCGC 3 cut(s) 64, 375, 384
Hin1II CATG 3 cut(s) 133, 356, 555
Hin6I GCGC 3 cut(s) 62, 373, 382
HinP1I GCGC 3 cut(s) 62, 373, 382
HindIII AAGCTT 1 cut(s) 421
HinfI GANTC 2 cut(s) 459, 606
HphI GGTGA 2 cut(s) 11, 608
Hpy188I TCNGA 2 cut(s) 464, 549
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4V TGCA 5 cut(s) 137, 182, 356, 536, 555
HpyF10VI GCNNNNNNNGC 6 cut(s) 250, 372, 379, 381, 420, 503
Hsp92II CATG 3 cut(s) 133, 356, 555
HspAI GCGC 3 cut(s) 62, 373, 382
Kzo9I GATC 5 cut(s) 12, 16, 403, 499, 544
LmnI GCTCC 1 cut(s) 144
LpnPI CCDG 8 cut(s) 168, 240, 267, 288, 329, 380, 383, 546
Lsp1109I GCAGC 2 cut(s) 101, 368
MaeI CTAG 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 119
MalI GATC 5 cut(s) 14, 18, 405, 501, 546
MbiI CCGCTC 1 cut(s) 506
MboI GATC 5 cut(s) 12, 16, 403, 499, 544
MboII GAAGA 3 cut(s) 163, 198, 208
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 1 cut(s) 8
MlyI GAGTC 1 cut(s) 600
MmeI TCCRAC 2 cut(s) 262, 572
Mph1103I ATGCAT 1 cut(s) 557
MseI TTAA 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 255
MunI CAATTG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 6 cut(s) 250, 372, 379, 381, 420, 503
NdeII GATC 5 cut(s) 12, 16, 403, 499, 544
NlaIII CATG 3 cut(s) 133, 356, 555
NlaIV GGNNCC 2 cut(s) 367, 531
NsiI ATGCAT 1 cut(s) 557
NspI RCATGY 2 cut(s) 133, 555
PciI ACATGT 1 cut(s) 129
PfeI GAWTC 1 cut(s) 459
PkrI GCNGC 4 cut(s) 91, 358, 386, 508
Ple19I CGATCG 1 cut(s) 502
PleI GAGTC 1 cut(s) 600
PpsI GAGTC 1 cut(s) 600
PpuMI RGGWCCY 1 cut(s) 530
PscI ACATGT 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 530
Psp6I CCWGG 1 cut(s) 253
PspFI CCCAGC 2 cut(s) 343, 369
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 367, 531
PspPI GGNCC 3 cut(s) 365, 397, 530
PspPPI RGGWCCY 1 cut(s) 530
PstI CTGCAG 2 cut(s) 184, 538
PsuI RGATCY 1 cut(s) 403
PvuI CGATCG 1 cut(s) 502
RsaI GTAC 2 cut(s) 349, 483
RsaNI GTAC 2 cut(s) 348, 482
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 4 cut(s) 90, 357, 385, 507
Sau3AI GATC 5 cut(s) 12, 16, 403, 499, 544
Sau96I GGNCC 3 cut(s) 365, 397, 530
SchI GAGTC 1 cut(s) 600
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 416
SetI ASST 7 cut(s) 24, 72, 177, 297, 425, 543, 616
SfcI CTRYAG 2 cut(s) 180, 534
SinI GGWCC 1 cut(s) 530
Sse9I AATT 1 cut(s) 8
SsiI CCGC 3 cut(s) 385, 495, 506
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 2 cut(s) 338, 432
TaaI ACNGT 1 cut(s) 322
TaqI TCGA 3 cut(s) 15, 502, 543
TasI AATT 1 cut(s) 8
TatI WGTACW 1 cut(s) 481
TauI GCSGC 2 cut(s) 387, 509
TfiI GAWTC 1 cut(s) 459
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseI GCWGC 2 cut(s) 89, 356
TspGWI ACGGA 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 530
XagI CCTNNNNNAGG 1 cut(s) 392
XceI RCATGY 2 cut(s) 133, 555
XspI CTAG 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.