pycom10g18560

Belongs to the isocitrate and isopropylmalate dehydrogenases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
21806801 .. 21807438
638 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 309 bp
ATGAAAACTTGGATTTGGTTCCAGGTGTGCCGAGATGGAAAGACCATCGAAGCTGAAGCAGCCCATGGTACAGTAACCCACCATTACCGGGTCCACCAGAAAGGAGGCGAAACAAGTACAAACAGCATAGCTTCGATCTTTGCATGGTCACGAGGTCTTGCACACAGGGTAACTAGGTCTCATCATCTGAATACCGAAGAATTCATTGACGCCGTGGCTGATGAGTTGAGAGTTAGATTGTCTAGCAGAGACAAAGTTGCAATCTCCGTTTGGTTACAAGTACACGTTACCAGGCCTGGAAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146
Pfam Domains
Protein Families
Iso_dh

Protein Analysis

103

Amino Acids

11.61

Weight (kDa)

9.51

Isoelectric Point (pI)

23.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 13 - 84 7.4e-06 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 3 cut(s) 70, 118, 282
AfiI CCNNNNNNNGG 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 283
AjnI CCWGG 3 cut(s) 21, 290, 295
AluBI AGCT 2 cut(s) 53, 131
AluI AGCT 2 cut(s) 53, 131
Alw26I GTCTC 2 cut(s) 183, 243
AoxI GGCC 1 cut(s) 293
ApeKI GCWGC 1 cut(s) 59
ApoI RAATTY 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 91
AsuC2I CCSGG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 91
BauI CACGAG 1 cut(s) 150
BbvI GCAGC 1 cut(s) 71
BccI CCATC 2 cut(s) 29, 53
BceAI ACGGC 1 cut(s) 197
BciT130I CCWGG 3 cut(s) 23, 292, 297
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 2 cut(s) 183, 243
BfaI CTAG 2 cut(s) 174, 243
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
Bme1390I CCNGG 4 cut(s) 23, 89, 292, 297
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 20, 92
BmrFI CCNGG 4 cut(s) 23, 89, 292, 297
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 64, 213
Bsc4I CCNNNNNNNGG 1 cut(s) 88
BseBI CCWGG 3 cut(s) 23, 292, 297
BseDI CCNNGG 2 cut(s) 64, 213
BseLI CCNNNNNNNGG 1 cut(s) 88
BseXI GCAGC 1 cut(s) 71
BshFI GGCC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 2 cut(s) 183, 243
BsnI GGCC 1 cut(s) 295
Bso31I GGTCTC 1 cut(s) 183
Bsp143I GATC 1 cut(s) 135
Bsp19I CCATGG 1 cut(s) 64
BspANI GGCC 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 20, 92
BspTNI GGTCTC 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 64, 213
BssMI GATC 1 cut(s) 135
BssNI GRCGYC 1 cut(s) 210
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 64
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 3 cut(s) 23, 292, 297
Bst4CI ACNGT 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 210
BstDSI CCRYGG 2 cut(s) 64, 213
BstKTI GATC 1 cut(s) 138
BstMAI GTCTC 2 cut(s) 183, 243
BstMBI GATC 1 cut(s) 135
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 3 cut(s) 23, 292, 297
BstSCI CCNGG 4 cut(s) 21, 87, 290, 295
BstV1I GCAGC 1 cut(s) 71
BsuRI GGCC 1 cut(s) 295
BtgI CCRYGG 2 cut(s) 64, 213
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 1 cut(s) 218
Csp6I GTAC 3 cut(s) 69, 117, 281
CviAII CATG 2 cut(s) 65, 144
CviJI RGCY 5 cut(s) 53, 62, 131, 218, 295
CviKI_1 RGCY 5 cut(s) 53, 62, 131, 218, 295
CviQI GTAC 3 cut(s) 69, 117, 281
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 64
Eco147I AGGCCT 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 75
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 3 cut(s) 21, 290, 295
EcoT14I CCWWGG 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 2 cut(s) 68, 147
FaiI YATR 3 cut(s) 66, 128, 145
FatI CATG 2 cut(s) 64, 143
Fnu4HI GCNGC 1 cut(s) 60
Fsp4HI GCNGC 1 cut(s) 60
FspBI CTAG 2 cut(s) 174, 243
GluI GCNGC 1 cut(s) 60
HaeIII GGCC 1 cut(s) 295
HapII CCGG 1 cut(s) 88
HgaI GACGC 1 cut(s) 218
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 2 cut(s) 68, 147
HpaII CCGG 1 cut(s) 88
Hpy166II GTNNAC 2 cut(s) 94, 283
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 2 cut(s) 94, 283
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 3 cut(s) 143, 161, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 2 cut(s) 68, 147
Kzo9I GATC 1 cut(s) 135
LpnPI CCDG 8 cut(s) 8, 35, 101, 110, 151, 277, 282, 304
Lsp1109I GCAGC 1 cut(s) 71
MaeI CTAG 2 cut(s) 174, 243
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 5 cut(s) 73, 147, 169, 273, 286
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 1 cut(s) 209
MluCI AATT 2 cut(s) 200, 304
MnlI CCTC 2 cut(s) 98, 146
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 4 cut(s) 23, 89, 292, 297
MvaI CCWGG 3 cut(s) 23, 292, 297
MwoI GCNNNNNNNGC 1 cut(s) 59
NciI CCSGG 1 cut(s) 89
NcoI CCATGG 1 cut(s) 64
NdeII GATC 1 cut(s) 135
NlaIII CATG 2 cut(s) 68, 147
NlaIV GGNNCC 2 cut(s) 20, 92
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 147
PceI AGGCCT 1 cut(s) 295
PkrI GCNGC 1 cut(s) 61
Psp6I CCWGG 3 cut(s) 21, 290, 295
PspGI CCWGG 3 cut(s) 21, 290, 295
PspN4I GGNNCC 2 cut(s) 20, 92
PspPI GGNCC 1 cut(s) 91
RsaI GTAC 3 cut(s) 70, 118, 282
RsaNI GTAC 3 cut(s) 69, 117, 281
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 1 cut(s) 91
ScrFI CCNGG 4 cut(s) 23, 89, 292, 297
SetI ASST 6 cut(s) 27, 55, 133, 157, 179, 288
SinI GGWCC 1 cut(s) 91
Sse9I AATT 2 cut(s) 200, 304
SseBI AGGCCT 1 cut(s) 295
SspMI CTAG 2 cut(s) 174, 243
StuI AGGCCT 1 cut(s) 295
StyD4I CCNGG 4 cut(s) 21, 87, 290, 295
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 288
TaqI TCGA 2 cut(s) 48, 134
TasI AATT 2 cut(s) 200, 304
TatI WGTACW 2 cut(s) 116, 280
TseFI GTSAC 1 cut(s) 147
TseI GCWGC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 147
TspDTI ATGAA 2 cut(s) 17, 193
TspGWI ACGGA 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 1 cut(s) 200
XspI CTAG 2 cut(s) 174, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.