pycom10g20440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
23330324 .. 23333339
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1620 bp
ATGATTATGGATGTTTTGTCAAACATGCACTCAACCTGTTCGATGAAAGTCCGCAACCAATTAGCAGTTGATGTAATTATTAAGCTCAAAGAATCCACATACAGTTTGTTGCAGTGCATATTTTGGATGATCAGATACTCAAACTATAATCTTCCCATACATTTTGTTCCCCTAAACCAGAGCTCCCCAACGGCACTGACTTTGGTTGCTGATTGCAGGTTCACATACAGATGTGAATGGCGGATCAAATTCCAAGATACCAACGGGGAAGCTACTCAGTTTACTGGTATACTTGCTTGTTGGAGGTTGTTAAGAGGGAATGCTTTACTAGCCAAGACTACGATCCATTGTATGATCTCAAGCTTGTGGCCTGTACTTTCAGAAACCACTGGTGCAGTAAGGAAGTCGGGGGGCAACTTTAAGGAAAAATTACGAGAGCTTGGAGGTCTTGATGCTGTATTTGAGGTGACCATGGGTTGCCATTCCAATATGGAGGGCTGGCTAAAAGATAGCTCGCATTCTATTTGGGAAAATGAAAACGGCATGGTGCGGAGCCTGGTTCTGCTTTTGAAATGTTTGAAGATCATGGATAATGCCATATTCCTAAGTAAAGAGAATCAGGTATTTAAATGTGGTTTAGCCTCCCTCTCTATCCCTCCCTCCTCAGTCCGCTGGTCTGCTTTCTCTCTTTCTCACGCTTTTCTTCTCCTTTTTAGACTTTACAATGAAGTGATTGAGCTTAAGGGCATTATCCGATTTTTCTGCAGATGTAGACCTTTGAATCAAAATGAAATTTCAAGTGGATCTGGTTTCGTTGTTGAATTCGAATCCTCCCTAAATAACGAGGAGATGATGGAACTGCAGATCATTTGTTCTGATTCCTCAAAAAAGCAGTTCAAGTTTGACCATACCGATTTTGTAATTGCACTGTCTAATACTCCACAGTTGATTTTGTTACGATGGATAACAGTGCTCTGTTTTTTTGTTGTTGATATTTTCGGTCTATGTGTGCAGCAAGCAAAATCCTTTCATTCATATGAGCTTCTTGGGCCTCTTTACCTTCACAGCAGCTTACCTAAAATGCTTTTTCAATATGTCGATGTGTCTGAAGCGTCTATGAGAATCTCAAACTTAATAAATAATTTCCTGCAGATCAAAAAGCTTGATGGCTCTGAGTTCCGAAATGCTTTTGACCGCCAATCTGTTCGTTGGGGCTTTTTAGGGTTCAGCTATTTGATTTCACAGATTCATTTCAGGCACAGTTGGTCAAGGACACAGGGAGAAGAGCACTTTGGAAATGGTAAGGTGCGCAAATCTGGGAAAAGCATGACAGGGGAAAGCTGGGATATTGTTTTGGATGAGGAGACCTATTACGAGGCTGAGCCGCATTACGGGTGGGCAGATGTCTTTGGTGACTCTAGCCAATTGCTGTCAATTAAACCTCGAGAATGGCCACCTGGCGTCTACCCTGCCCTTGATCTTGGATCATCGTCGCCTTCACTTGATGAACCACCAGATGAATTTTCTCCAATATCGTGCGGCTCAGAGTTTTTTGTATGTGTTTATCCGTATTTTTGTGCTCTGAATAATTCTAATCAAATTCCATTGCTCCTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

540

Amino Acids

61.45

Weight (kDa)

6.25

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 143 - 208 3e-06 Wings apart-like protein regulation of heterochromatin
Microtub_bd PF16796 239 - 303 1.8e-06 Microtubule binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1310
Acc36I ACCTGC 1 cut(s) 207
AccI GTMKAC 3 cut(s) 289, 772, 1464
AciI CCGC 7 cut(s) 52, 241, 550, 670, 1195, 1385, 1539
AclWI GGATC 4 cut(s) 251, 337, 811, 1492
AcoI YGGCCR 1 cut(s) 1451
AcsI RAATTY 5 cut(s) 248, 792, 821, 1520, 1599
AcuI CTGAAG 1 cut(s) 1128
AcyI GRCGYC 1 cut(s) 1461
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 1391
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 7 cut(s) 571, 580, 781, 798, 821, 898, 1091
AjnI CCWGG 2 cut(s) 555, 1456
AjuI GAANNNNNNNTTGG 2 cut(s) 1275, 1307
Alw21I GWGCWC 4 cut(s) 185, 975, 1290, 1582
Alw26I GTCTC 1 cut(s) 1358
AlwI GGATC 4 cut(s) 251, 337, 811, 1492
Ama87I CYCGRG 1 cut(s) 1443
AoxI GGCC 3 cut(s) 368, 1049, 1451
ApeKI GCWGC 2 cut(s) 1012, 1068
ApoI RAATTY 5 cut(s) 248, 792, 821, 1520, 1599
AspLEI GCGC 1 cut(s) 1311
AspS9I GGNCC 1 cut(s) 1049
AsuHPI GGTGA 2 cut(s) 478, 1424
AsuII TTCGAA 1 cut(s) 825
AvaI CYCGRG 1 cut(s) 1443
BalI TGGCCA 1 cut(s) 1453
BanII GRGCYC 1 cut(s) 185
Bbv12I GWGCWC 4 cut(s) 185, 975, 1290, 1582
BbvI GCAGC 2 cut(s) 1024, 1080
BccI CCATC 3 cut(s) 847, 954, 1160
BceAI ACGGC 2 cut(s) 207, 556
BcgI CGANNNNNNTGC 2 cut(s) 744, 778
BciT130I CCWGG 2 cut(s) 557, 1458
BclI TGATCA 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 1358
BfaI CTAG 2 cut(s) 329, 1419
BfmI CTRYAG 3 cut(s) 763, 860, 1148
BfrI CTTAAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 207
BisI GCNGC 4 cut(s) 1013, 1069, 1385, 1540
BlpI GCTNAGC 1 cut(s) 1380
BlsI GCNGC 4 cut(s) 1014, 1070, 1386, 1541
Bme1390I CCNGG 2 cut(s) 557, 1458
BmeT110I CYCGRG 1 cut(s) 1443
BmgT120I GGNCC 1 cut(s) 1049
BmiI GGNNCC 1 cut(s) 554
BmrFI CCNGG 2 cut(s) 557, 1458
BmsI GCATC 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 1613
Bpu1102I GCTNAGC 1 cut(s) 1380
Bpu14I TTCGAA 1 cut(s) 825
BpuEI CTTGAG 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 1461
BsaI GGTCTC 1 cut(s) 1358
BsaJI CCNNGG 1 cut(s) 471
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 1391
Bse1I ACTGG 2 cut(s) 289, 394
Bse3DI GCAATG 1 cut(s) 1604
BseBI CCWGG 2 cut(s) 557, 1458
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 3 cut(s) 16, 132, 1363
BseLI CCNNNNNNNGG 1 cut(s) 1391
BseMI GCAATG 1 cut(s) 1604
BseMII CTCAG 5 cut(s) 290, 678, 1164, 1371, 1557
BseNI ACTGG 2 cut(s) 289, 394
BseRI GAGGAG 3 cut(s) 652, 860, 1376
BseXI GCAGC 2 cut(s) 1024, 1080
BseYI CCCAGC 1 cut(s) 1341
BsgI GTGCAG 2 cut(s) 414, 1031
BshFI GGCC 3 cut(s) 370, 1051, 1453
BsiHKAI GWGCWC 4 cut(s) 185, 975, 1290, 1582
BsiHKCI CYCGRG 1 cut(s) 1443
BslI CCNNNNNNNGG 1 cut(s) 1391
BsmAI GTCTC 1 cut(s) 1358
BsmI GAATGC 2 cut(s) 325, 517
BsnI GGCC 3 cut(s) 370, 1051, 1453
Bso31I GGTCTC 1 cut(s) 1358
BsoBI CYCGRG 1 cut(s) 1443
Bsp119I TTCGAA 1 cut(s) 825
Bsp1286I GDGCHC 4 cut(s) 185, 975, 1290, 1582
Bsp1720I GCTNAGC 1 cut(s) 1380
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 7 cut(s) 52, 241, 550, 670, 1195, 1385, 1539
BspANI GGCC 3 cut(s) 370, 1051, 1453
BspCNI CTCAG 5 cut(s) 289, 677, 1165, 1372, 1556
BspLI GGNNCC 1 cut(s) 554
BspMAI CTGCAG 3 cut(s) 767, 864, 1152
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 4 cut(s) 251, 337, 811, 1492
BspQI GCTCTTC 1 cut(s) 1278
BspT104I TTCGAA 1 cut(s) 825
BspTI CTTAAG 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 1358
BsrDI GCAATG 1 cut(s) 1604
BsrI ACTGG 2 cut(s) 289, 394
BssECI CCNNGG 1 cut(s) 471
BssNAI GTATAC 1 cut(s) 290
BssNI GRCGYC 1 cut(s) 1461
BssT1I CCWWGG 1 cut(s) 471
Bst1107I GTATAC 1 cut(s) 290
Bst2UI CCWGG 2 cut(s) 557, 1458
Bst4CI ACNGT 5 cut(s) 104, 930, 945, 970, 1262
Bst6I CTCTTC 1 cut(s) 1278
BstACI GRCGYC 1 cut(s) 1461
BstAFI CTTAAG 1 cut(s) 740
BstBI TTCGAA 1 cut(s) 825
BstC8I GCNNGC 3 cut(s) 500, 515, 1017
BstDEI CTNAG 7 cut(s) 276, 605, 664, 1173, 1380, 1543, 1613
BstDSI CCRYGG 1 cut(s) 471
BstEII GGTNACC 1 cut(s) 466
BstF5I GGATG 3 cut(s) 16, 132, 1363
BstHHI GCGC 1 cut(s) 1311
BstMAI GTCTC 1 cut(s) 1358
BstMWI GCNNNNNNNGC 2 cut(s) 329, 1048
BstNI CCWGG 2 cut(s) 557, 1458
BstNSI RCATGY 1 cut(s) 28
BstPI GGTNACC 1 cut(s) 466
BstSCI CCNGG 2 cut(s) 555, 1456
BstSFI CTRYAG 3 cut(s) 763, 860, 1148
BstV1I GCAGC 2 cut(s) 1024, 1080
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BstZ17I GTATAC 1 cut(s) 290
BsuRI GGCC 3 cut(s) 370, 1051, 1453
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 3 cut(s) 16, 132, 1363
BtsI GCAGTG 1 cut(s) 119
BtsIMutI CAGTG 5 cut(s) 119, 194, 387, 926, 975
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 3 cut(s) 500, 515, 1017
CfoI GCGC 1 cut(s) 1311
Cfr13I GGNCC 1 cut(s) 1049
CseI GACGC 2 cut(s) 1101, 1450
Csp6I GTAC 1 cut(s) 374
CviAII CATG 5 cut(s) 25, 472, 544, 586, 1327
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 7 cut(s) 276, 605, 664, 1173, 1380, 1543, 1613
DraI TTTAAA 1 cut(s) 628
EaeI YGGCCR 1 cut(s) 1451
Eam1104I CTCTTC 1 cut(s) 1278
EarI CTCTTC 1 cut(s) 1278
EciI GGCGGA 1 cut(s) 256
Ecl136II GAGCTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 1358
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 1128
Eco88I CYCGRG 1 cut(s) 1443
Eco91I GGTNACC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 183
EcoO65I GGTNACC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 821
EcoRII CCWGG 2 cut(s) 555, 1456
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 5 cut(s) 28, 475, 547, 589, 1330
FatI CATG 5 cut(s) 24, 471, 543, 585, 1326
FauNDI CATATG 1 cut(s) 1036
FbaI TGATCA 1 cut(s) 129
FblI GTMKAC 3 cut(s) 289, 772, 1464
Fnu4HI GCNGC 4 cut(s) 1013, 1069, 1385, 1540
FokI GGATG 3 cut(s) 23, 139, 1370
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 4 cut(s) 1013, 1069, 1385, 1540
FspBI CTAG 2 cut(s) 329, 1419
FspI TGCGCA 1 cut(s) 1310
GlaI GCGC 1 cut(s) 1310
GluI GCNGC 4 cut(s) 1013, 1069, 1385, 1540
GsaI CCCAGC 1 cut(s) 1345
HaeIII GGCC 3 cut(s) 370, 1051, 1453
HgaI GACGC 2 cut(s) 1101, 1450
HhaI GCGC 1 cut(s) 1311
Hin1I GRCGYC 1 cut(s) 1461
Hin1II CATG 5 cut(s) 28, 475, 547, 589, 1330
Hin6I GCGC 1 cut(s) 1309
HinP1I GCGC 1 cut(s) 1309
HindIII AAGCTT 2 cut(s) 361, 1160
HinfI GANTC 8 cut(s) 92, 616, 781, 827, 878, 1122, 1246, 1415
HphI GGTGA 2 cut(s) 478, 1424
Hpy166II GTNNAC 5 cut(s) 222, 282, 290, 773, 1465
Hpy188I TCNGA 9 cut(s) 134, 382, 755, 877, 1108, 1174, 1181, 1546, 1584
Hpy188III TCNNGA 2 cut(s) 449, 1445
Hpy8I GTNNAC 5 cut(s) 222, 282, 290, 773, 1465
Hpy99I CGWCG 1 cut(s) 1495
HpyAV CCTTC 2 cut(s) 1070, 1506
HpyCH4III ACNGT 5 cut(s) 104, 930, 945, 970, 1262
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 1048
HpyF3I CTNAG 7 cut(s) 276, 605, 664, 1173, 1380, 1543, 1613
Hsp92I GRCGYC 1 cut(s) 1461
Hsp92II CATG 5 cut(s) 28, 475, 547, 589, 1330
HspAI GCGC 1 cut(s) 1309
Ksp22I TGATCA 1 cut(s) 129
LguI GCTCTTC 1 cut(s) 1278
LmnI GCTCC 3 cut(s) 188, 552, 1614
Lsp1109I GCAGC 2 cut(s) 1024, 1080
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 329, 1419
MaeIII GTNAC 3 cut(s) 466, 954, 1412
MboII GAAGA 4 cut(s) 143, 592, 695, 1295
MfeI CAATTG 1 cut(s) 1424
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 4 cut(s) 185, 975, 1290, 1582
MlsI TGGCCA 1 cut(s) 1453
MluNI TGGCCA 1 cut(s) 1453
MlyI GAGTC 1 cut(s) 1409
MmeI TCCRAC 1 cut(s) 281
Mox20I TGGCCA 1 cut(s) 1453
MscI TGGCCA 1 cut(s) 1453
MseI TTAA 7 cut(s) 81, 311, 420, 627, 741, 1133, 1437
MslI CAYNNNNRTG 2 cut(s) 229, 1035
Msp20I TGGCCA 1 cut(s) 1453
MspA1I CMGCKG 1 cut(s) 672
MspCI CTTAAG 1 cut(s) 740
MspR9I CCNGG 2 cut(s) 557, 1458
MunI CAATTG 1 cut(s) 1424
Mva1269I GAATGC 2 cut(s) 325, 517
MvaI CCWGG 2 cut(s) 557, 1458
MwoI GCNNNNNNNGC 2 cut(s) 329, 1048
NcoI CCATGG 1 cut(s) 471
NdeI CATATG 1 cut(s) 1036
NlaIII CATG 5 cut(s) 28, 475, 547, 589, 1330
NlaIV GGNNCC 1 cut(s) 554
NmuCI GTSAC 2 cut(s) 466, 1412
NsbI TGCGCA 1 cut(s) 1310
NspI RCATGY 1 cut(s) 28
NspV TTCGAA 1 cut(s) 825
PaeR7I CTCGAG 1 cut(s) 1443
PciSI GCTCTTC 1 cut(s) 1278
PctI GAATGC 2 cut(s) 325, 517
PfeI GAWTC 7 cut(s) 92, 616, 781, 827, 878, 1122, 1246
PkrI GCNGC 4 cut(s) 1014, 1070, 1386, 1541
PleI GAGTC 1 cut(s) 1409
PpsI GAGTC 1 cut(s) 1409
Psp124BI GAGCTC 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 555, 1456
PspEI GGTNACC 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 1341
PspGI CCWGG 2 cut(s) 555, 1456
PspN4I GGNNCC 1 cut(s) 554
PspPI GGNCC 1 cut(s) 1049
PstI CTGCAG 3 cut(s) 767, 864, 1152
PsuI RGATCY 1 cut(s) 803
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 2 cut(s) 229, 1035
SacI GAGCTC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 1278
SaqAI TTAA 7 cut(s) 81, 311, 420, 627, 741, 1133, 1437
SatI GCNGC 4 cut(s) 1013, 1069, 1385, 1540
Sau96I GGNCC 1 cut(s) 1049
SchI GAGTC 1 cut(s) 1409
ScrFI CCNGG 2 cut(s) 557, 1458
SduI GDGCHC 4 cut(s) 185, 975, 1290, 1582
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 3 cut(s) 763, 860, 1148
Sfr274I CTCGAG 1 cut(s) 1443
SfuI TTCGAA 1 cut(s) 825
SlaI CTCGAG 1 cut(s) 1443
SmiI ATTTAAAT 1 cut(s) 628
SmiMI CAYNNNNRTG 2 cut(s) 229, 1035
SmlI CTYRAG 3 cut(s) 358, 740, 1443
SmoI CTYRAG 3 cut(s) 358, 740, 1443
SsiI CCGC 7 cut(s) 52, 241, 550, 670, 1195, 1385, 1539
SspMI CTAG 2 cut(s) 329, 1419
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 555, 1456
StyI CCWWGG 1 cut(s) 471
SwaI ATTTAAAT 1 cut(s) 628
TaaI ACNGT 5 cut(s) 104, 930, 945, 970, 1262
TaqI TCGA 4 cut(s) 41, 825, 1098, 1444
TaqII GACCGA 1 cut(s) 989
TatI WGTACW 1 cut(s) 373
TauI GCSGC 2 cut(s) 1387, 1542
TfiI GAWTC 7 cut(s) 92, 616, 781, 827, 878, 1122, 1246
Tru1I TTAA 7 cut(s) 81, 311, 420, 627, 741, 1133, 1437
Tru9I TTAA 7 cut(s) 81, 311, 420, 627, 741, 1133, 1437
TscAI CASTG 5 cut(s) 119, 201, 394, 933, 975
TseFI GTSAC 2 cut(s) 466, 1412
TseI GCWGC 2 cut(s) 1012, 1068
Tsp45I GTSAC 2 cut(s) 466, 1412
TspDTI ATGAA 9 cut(s) 59, 549, 741, 804, 1019, 1023, 1238, 1521, 1533
TspGWI ACGGA 1 cut(s) 1557
TspRI CASTG 5 cut(s) 119, 201, 394, 933, 975
Vha464I CTTAAG 1 cut(s) 740
XapI RAATTY 5 cut(s) 248, 792, 821, 1520, 1599
XceI RCATGY 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 1443
XmiI GTMKAC 3 cut(s) 289, 772, 1464
XspI CTAG 2 cut(s) 329, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.