pycom10g20800

Small RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
23593009 .. 23593473
465 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGCTGTGCCAACATATTCATAATGTTGGGCATAAAGAGACCGAAGATGGCAAATTAGCGTATTCCAACTCTGTGAATGCGTCATTTATTCTCTCTGGAATTGAACTTCGTTCTTTAAGTATAGAAGGACCGGATTCTGGTGTTTTTCCATCTATTGGGTCCTTAGCATGTGCGAGTTATTCTGTTTCTTTGGTGACTGAAGGAAAACATATGAAAGAACTTTTGGAAAGTAGTGATGACTTTGAGTTCGAGATAGCATCTGGAGCAGTGATTCCACATCTCGAGTCAGTTATCACACAAATGACTGTTGGTCAATCGGCTTTTTTCAGTATGGATTTGCCACCTCAAGAGTTGGTTTTTGCTGCAGCTGGCAATTCTGAAAAAAGCCTGCTCCATTCTATCCTCCAGTGGTTCGGTTACCTCCGGGAGATGATCTTCCTATGTTGCAGATTTAGAACATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001510 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009653 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010093 GO:0010305 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016740 GO:0016741 GO:0019222 GO:0022414 GO:0031047 GO:0031050 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035279 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048519 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0098542 GO:0098586 GO:0098795 GO:0099402 GO:0140098 GO:1901360 GO:1901698 GO:1901699 GO:1905392 GO:1905393 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKBP_C Methyltransf_11 Methyltransf_12 Methyltransf_31 dsrm

Protein Analysis

155

Amino Acids

16.87

Weight (kDa)

4.98

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 155
AcuI CTGAAG 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 137, 155
AgsI TTSAA 1 cut(s) 104
AhdI GACNNNNNGTC 1 cut(s) 309
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 1 cut(s) 368
AluI AGCT 1 cut(s) 368
Alw26I GTCTC 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 281
ApeKI GCWGC 2 cut(s) 362, 365
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AspS9I GGNCC 2 cut(s) 128, 159
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 2 cut(s) 128, 159
BbvI GCAGC 2 cut(s) 349, 377
BccI CCATC 2 cut(s) 41, 157
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 2 cut(s) 363, 366
BlsI GCNGC 2 cut(s) 364, 367
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 2 cut(s) 128, 159
BmeRI GACNNNNNGTC 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 128, 159
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 266
BpmI CTGGAG 2 cut(s) 282, 389
Bpu10I CCTNAGC 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 32
BsaWI WCCGGW 1 cut(s) 130
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 155
Bse1I ACTGG 1 cut(s) 406
BseLI CCNNNNNNNGG 2 cut(s) 137, 155
BseNI ACTGG 1 cut(s) 406
BseXI GCAGC 2 cut(s) 349, 377
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 131, 424
BslI CCNNNNNNNGG 2 cut(s) 137, 155
BsmAI GTCTC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 281
Bsp143I GATC 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 160
BspMAI CTGCAG 1 cut(s) 367
BspTNI GGTCTC 1 cut(s) 32
BsrI ACTGG 1 cut(s) 406
BssMI GATC 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 307
BstC8I GCNNGC 2 cut(s) 370, 389
BstDEI CTNAG 1 cut(s) 163
BstEII GGTNACC 1 cut(s) 416
BstKTI GATC 1 cut(s) 435
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 1 cut(s) 432
BstMWI GCNNNNNNNGC 1 cut(s) 263
BstNSI RCATGY 1 cut(s) 171
BstPI GGTNACC 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 423
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 2 cut(s) 349, 377
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 273, 413
Cac8I GCNNGC 2 cut(s) 370, 389
Cfr13I GGNCC 2 cut(s) 128, 159
CseI GACGC 1 cut(s) 69
CviAII CATG 1 cut(s) 168
CviJI RGCY 3 cut(s) 320, 368, 387
CviKI_1 RGCY 3 cut(s) 320, 368, 387
DdeI CTNAG 1 cut(s) 163
DpnI GATC 1 cut(s) 434
DpnII GATC 1 cut(s) 432
DriI GACNNNNNGTC 1 cut(s) 309
Eam1105I GACNNNNNGTC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 2 cut(s) 128, 159
Eco57I CTGAAG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 281
Eco91I GGTNACC 1 cut(s) 416
EcoO109I RGGNCCY 1 cut(s) 159
EcoO65I GGTNACC 1 cut(s) 416
FaeI CATG 1 cut(s) 171
FaiI YATR 9 cut(s) 15, 21, 33, 122, 169, 210, 212, 332, 442
FatI CATG 1 cut(s) 167
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 2 cut(s) 363, 366
Fsp4HI GCNGC 2 cut(s) 363, 366
GluI GCNGC 2 cut(s) 363, 366
GsuI CTGGAG 2 cut(s) 282, 389
HapII CCGG 2 cut(s) 131, 424
HgaI GACGC 1 cut(s) 69
Hin1II CATG 1 cut(s) 171
HinfI GANTC 3 cut(s) 134, 271, 284
HpaII CCGG 2 cut(s) 131, 424
HphI GGTGA 1 cut(s) 205
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 5 cut(s) 96, 250, 261, 281, 347
HpyAV CCTTC 2 cut(s) 119, 194
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4V TGCA 2 cut(s) 365, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 263
HpyF3I CTNAG 1 cut(s) 163
Hsp92II CATG 1 cut(s) 171
Kzo9I GATC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 263, 396
LpnPI CCDG 8 cut(s) 81, 123, 144, 246, 354, 401, 419, 437
Lsp1109I GCAGC 2 cut(s) 349, 377
LweI GCATC 1 cut(s) 266
MaeIII GTNAC 2 cut(s) 193, 416
MalI GATC 1 cut(s) 434
MboI GATC 1 cut(s) 432
MboII GAAGA 2 cut(s) 56, 427
MluCI AATT 3 cut(s) 53, 99, 373
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 3 cut(s) 354, 413, 431
MseI TTAA 2 cut(s) 116, 463
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 2 cut(s) 131, 424
MspR9I CCNGG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 82
MwoI GCNNNNNNNGC 1 cut(s) 263
NciI CCSGG 1 cut(s) 425
NdeI CATATG 1 cut(s) 210
NdeII GATC 1 cut(s) 432
NlaIII CATG 1 cut(s) 171
NlaIV GGNNCC 1 cut(s) 160
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 281
PctI GAATGC 1 cut(s) 82
PfeI GAWTC 2 cut(s) 134, 271
PflMI CCANNNNNTGG 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 423
PkrI GCNGC 2 cut(s) 364, 367
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PpuMI RGGWCCY 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 159
PspEI GGTNACC 1 cut(s) 416
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 2 cut(s) 128, 159
PspPPI RGGWCCY 1 cut(s) 159
PstI CTGCAG 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 368
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 2 cut(s) 116, 463
SatI GCNGC 2 cut(s) 363, 366
Sau3AI GATC 1 cut(s) 432
Sau96I GGNCC 2 cut(s) 128, 159
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 425
SetI ASST 3 cut(s) 346, 370, 423
SfaNI GCATC 1 cut(s) 266
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 281
SinI GGWCC 2 cut(s) 128, 159
SlaI CTCGAG 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 281, 345
SmoI CTYRAG 2 cut(s) 281, 345
Sse9I AATT 3 cut(s) 53, 99, 373
StyD4I CCNGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 307
TaqI TCGA 2 cut(s) 249, 282
TaqII GACCGA 1 cut(s) 56
TasI AATT 3 cut(s) 53, 99, 373
TfiI GAWTC 2 cut(s) 134, 271
Tru1I TTAA 2 cut(s) 116, 463
Tru9I TTAA 2 cut(s) 116, 463
TscAI CASTG 2 cut(s) 273, 413
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 2 cut(s) 362, 365
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 8, 227
TspRI CASTG 2 cut(s) 273, 413
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 2 cut(s) 128, 159
XceI RCATGY 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.