pycom10g22560

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
24907085 .. 24907905
821 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 435 bp
ATGCACATGGTATGGCCTGCCTATAGTGAAGAGTGGTATTATAATGGTGGCTACAGTCAATTCAATCGGAGCTGTTTTCCACTGAGGATATCGGGATTGCTCGCAGCAGTTTCTGTTCTTTTTGCGGCTGTTGTACTTGTGAGCTTAAGACTTGTAGGCCATGAAAGGGAACTGTTCGTTGGATATATATGTGCTTTTTCAGTAGTTTCAGTGTATGCTTCGCCGTTGTTTATCATCTTCATGCCATTTAATCTTTCTCTTGCAACTTTCTTGGTGAGTTTCTCTTTCTGTGCATACGGAGCGTTGCAGCGCGACCCCTTTGTCTATATCCCAAACAGTCTCGGTACAATTTTAGGGTTTGTCCAGTTGGTTCTGTACTCCTACTATAGCAAAATGTCTGGAGATGATGACTCGAGAGAACCACTGCTTGCTTAA

Protein Analysis

145

Amino Acids

16.19

Weight (kDa)

5.51

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 59 - 131 1.3e-11 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 42
AasI GACNNNNNNGTC 1 cut(s) 320
AccII CGCG 1 cut(s) 312
AciI CCGC 1 cut(s) 125
AfaI GTAC 3 cut(s) 135, 346, 377
AfiI CCNNNNNNNGG 1 cut(s) 166
AflII CTTAAG 1 cut(s) 145
AgsI TTSAA 1 cut(s) 64
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 2 cut(s) 72, 144
AluI AGCT 2 cut(s) 72, 144
Alw26I GTCTC 1 cut(s) 344
AlwNI CAGNNNCTG 1 cut(s) 113
Ama87I CYCGRG 1 cut(s) 412
AoxI GGCC 2 cut(s) 14, 157
ApeKI GCWGC 2 cut(s) 104, 307
AspLEI GCGC 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 412
BbvI GCAGC 2 cut(s) 116, 319
BceAI ACGGC 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 344
BfmI CTRYAG 3 cut(s) 22, 52, 385
BfrI CTTAAG 1 cut(s) 145
BisI GCNGC 3 cut(s) 105, 126, 308
BlsI GCNGC 3 cut(s) 106, 127, 309
BmeT110I CYCGRG 1 cut(s) 412
BpmI CTGGAG 1 cut(s) 420
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 364
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 364
BseXI GCAGC 2 cut(s) 116, 319
Bsh1236I CGCG 1 cut(s) 312
BshFI GGCC 2 cut(s) 16, 159
BsiHKCI CYCGRG 1 cut(s) 412
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 344
BsnI GGCC 2 cut(s) 16, 159
BsoBI CYCGRG 1 cut(s) 412
BspACI CCGC 1 cut(s) 125
BspANI GGCC 2 cut(s) 16, 159
BspCNI CTCAG 1 cut(s) 75
BspFNI CGCG 1 cut(s) 312
BspTI CTTAAG 1 cut(s) 145
BsrI ACTGG 1 cut(s) 364
Bst4CI ACNGT 3 cut(s) 56, 174, 338
Bst6I CTCTTC 1 cut(s) 24
BstAFI CTTAAG 1 cut(s) 145
BstC8I GCNNGC 3 cut(s) 18, 102, 429
BstDEI CTNAG 1 cut(s) 83
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 344
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstSFI CTRYAG 3 cut(s) 22, 52, 385
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 2 cut(s) 116, 319
BsuRI GGCC 2 cut(s) 16, 159
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 3 cut(s) 80, 216, 422
Cac8I GCNNGC 3 cut(s) 18, 102, 429
CaiI CAGNNNCTG 1 cut(s) 113
CfoI GCGC 1 cut(s) 312
Csp6I GTAC 3 cut(s) 134, 345, 376
CviAII CATG 3 cut(s) 7, 161, 241
CviJI RGCY 6 cut(s) 16, 51, 72, 128, 144, 159
CviKI_1 RGCY 6 cut(s) 16, 51, 72, 128, 144, 159
CviQI GTAC 3 cut(s) 134, 345, 376
DdeI CTNAG 1 cut(s) 83
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Eco32I GATATC 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 412
EcoRV GATATC 1 cut(s) 90
FaeI CATG 3 cut(s) 10, 164, 244
FatI CATG 3 cut(s) 6, 160, 240
Fnu4HI GCNGC 3 cut(s) 105, 126, 308
Fsp4HI GCNGC 3 cut(s) 105, 126, 308
GlaI GCGC 1 cut(s) 311
GluI GCNGC 3 cut(s) 105, 126, 308
GsuI CTGGAG 1 cut(s) 420
HaeIII GGCC 2 cut(s) 16, 159
HhaI GCGC 1 cut(s) 312
Hin1II CATG 3 cut(s) 10, 164, 244
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HinfI GANTC 1 cut(s) 410
HphI GGTGA 1 cut(s) 286
Hpy188I TCNGA 1 cut(s) 69
Hpy188III TCNNGA 3 cut(s) 93, 399, 414
HpyCH4III ACNGT 3 cut(s) 56, 174, 338
HpyCH4V TGCA 4 cut(s) 4, 263, 293, 307
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 1 cut(s) 83
Hsp92II CATG 3 cut(s) 10, 164, 244
HspAI GCGC 1 cut(s) 310
LmnI GCTCC 2 cut(s) 69, 299
LpnPI CCDG 3 cut(s) 30, 377, 384
Lsp1109I GCAGC 2 cut(s) 116, 319
MboII GAAGA 2 cut(s) 41, 229
MluCI AATT 2 cut(s) 59, 348
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 1 cut(s) 78
MseI TTAA 3 cut(s) 146, 249, 433
MslI CAYNNNNRTG 1 cut(s) 239
MspCI CTTAAG 1 cut(s) 145
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 1 cut(s) 299
NlaIII CATG 3 cut(s) 10, 164, 244
PaeR7I CTCGAG 1 cut(s) 412
PkrI GCNGC 3 cut(s) 106, 127, 309
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PsiI TTATAA 1 cut(s) 42
PstNI CAGNNNCTG 1 cut(s) 113
RsaI GTAC 3 cut(s) 135, 346, 377
RsaNI GTAC 3 cut(s) 134, 345, 376
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 3 cut(s) 146, 249, 433
SatI GCNGC 3 cut(s) 105, 126, 308
SchI GAGTC 1 cut(s) 404
SetI ASST 2 cut(s) 74, 146
SfcI CTRYAG 3 cut(s) 22, 52, 385
Sfr274I CTCGAG 1 cut(s) 412
SlaI CTCGAG 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 145, 412
SmoI CTYRAG 2 cut(s) 145, 412
Sse9I AATT 2 cut(s) 59, 348
SsiI CCGC 1 cut(s) 125
TaaI ACNGT 3 cut(s) 56, 174, 338
TaqI TCGA 1 cut(s) 413
TasI AATT 2 cut(s) 59, 348
TatI WGTACW 2 cut(s) 133, 375
TauI GCSGC 1 cut(s) 128
Tru1I TTAA 3 cut(s) 146, 249, 433
Tru9I TTAA 3 cut(s) 146, 249, 433
TscAI CASTG 3 cut(s) 87, 216, 429
TseI GCWGC 2 cut(s) 104, 307
TspDTI ATGAA 2 cut(s) 177, 229
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 3 cut(s) 87, 216, 429
Vha464I CTTAAG 1 cut(s) 145
XhoI CTCGAG 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.