pycom10g23930

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Forward (+)
25890973 .. 25892707
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGGGGGCGTACAGAGCCGACGACGATTACGATTACTTGTTCAAGGTGGTGCTGATCGGCGACTCTGGCGTCGGAAAATCGAACCTTTTGTCCCGGTTCACGAGGAACGAGTTCAGCCTCGAATCCAAGTCCACCATTGGTGTCGAGTTCGCCACCAGAAGCATTCGGGTCGACGATAAGGTCGTCAAGGCCCAGATTTGGGACACCGCCGGCCAGGAAAGGTATCGTGCAATCACAAGCGCATACTATCGAGGCGCGGTTGGGGCATTACTTGTCTATGACGTTACTCGTCACGTTACATTTGAGAATGTGGAGAGATGGCTGAAGGAGCTTCGCGATCACACAGATGCCAACATTGTGATCATGCTAGTGGGAAACAAGGGATCGATCTGCGTCACTTGCGAGCTGTTTCCACTGATGACGCTAAGGCGTTTGCTGAACGAGAGAACACCTTTTTCATGGAAACGTCTGCCCTCGAGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ras

Protein Analysis

162

Amino Acids

18.41

Weight (kDa)

8.9

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 127 2.9e-10 ADP-ribosylation factor family
Ras PF00071 15 - 127 4e-47 Ras family
Roc PF08477 15 - 127 8.6e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 15 - 121 3.1e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 68, 179
AccI GTMKAC 1 cut(s) 171
AccII CGCG 2 cut(s) 257, 336
AciI CCGC 2 cut(s) 207, 257
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 198, 199
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 213
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 2 cut(s) 331, 406
AluI AGCT 2 cut(s) 331, 406
AlwI GGATC 1 cut(s) 391
Ama87I CYCGRG 1 cut(s) 475
AoxI GGCC 2 cut(s) 189, 211
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp700I GAANNNNTTC 1 cut(s) 110
AspLEI GCGC 2 cut(s) 242, 257
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 475
BauI CACGAG 1 cut(s) 100
BccI CCATC 1 cut(s) 312
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 1 cut(s) 215
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 1 cut(s) 94
BfaI CTAG 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 94, 215
BmeT110I CYCGRG 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 94, 215
BmsI GCATC 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 69
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 199
Bse118I RCCGGY 1 cut(s) 209
BseBI CCWGG 1 cut(s) 215
BseCI ATCGAT 1 cut(s) 386
BseLI CCNNNNNNNGG 2 cut(s) 198, 199
Bsh1236I CGCG 2 cut(s) 257, 336
BshFI GGCC 2 cut(s) 191, 213
BshVI ATCGAT 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 475
BsiSI CCGG 2 cut(s) 94, 210
BslFI GGGAC 2 cut(s) 76, 215
BslI CCNNNNNNNGG 2 cut(s) 198, 199
BsmFI GGGAC 2 cut(s) 76, 215
BsmI GAATGC 1 cut(s) 162
BsnI GGCC 2 cut(s) 191, 213
BsoBI CYCGRG 1 cut(s) 475
Bsp143I GATC 5 cut(s) 54, 337, 360, 383, 387
Bsp68I TCGCGA 1 cut(s) 336
BspACI CCGC 2 cut(s) 207, 257
BspANI GGCC 2 cut(s) 191, 213
BspDI ATCGAT 1 cut(s) 386
BspFNI CGCG 2 cut(s) 257, 336
BspPI GGATC 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssMI GATC 5 cut(s) 54, 337, 360, 383, 387
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 215
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 211, 404
BstDEI CTNAG 1 cut(s) 425
BstFNI CGCG 2 cut(s) 257, 336
BstHHI GCGC 2 cut(s) 242, 257
BstKTI GATC 5 cut(s) 57, 340, 363, 386, 390
BstMBI GATC 5 cut(s) 54, 337, 360, 383, 387
BstMWI GCNNNNNNNGC 5 cut(s) 14, 66, 263, 328, 399
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 2 cut(s) 92, 213
BstUI CGCG 2 cut(s) 257, 336
Bsu15I ATCGAT 1 cut(s) 386
BsuRI GGCC 2 cut(s) 191, 213
BsuTUI ATCGAT 1 cut(s) 386
BtsIMutI CAGTG 1 cut(s) 413
BtuMI TCGCGA 1 cut(s) 336
Cac8I GCNNGC 2 cut(s) 211, 404
CfoI GCGC 2 cut(s) 242, 257
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 190
ClaI ATCGAT 1 cut(s) 386
CseI GACGC 3 cut(s) 58, 382, 430
Csp6I GTAC 1 cut(s) 10
CviAII CATG 2 cut(s) 364, 459
CviJI RGCY 7 cut(s) 17, 117, 191, 213, 322, 331, 406
CviKI_1 RGCY 7 cut(s) 17, 117, 191, 213, 322, 331, 406
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 1 cut(s) 425
DpnI GATC 5 cut(s) 56, 339, 362, 385, 389
DpnII GATC 5 cut(s) 54, 337, 360, 383, 387
DrdI GACNNNNNNGTC 2 cut(s) 68, 179
DseDI GACNNNNNNGTC 2 cut(s) 68, 179
EaeI YGGCCR 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 213
FaeI CATG 2 cut(s) 367, 462
FaiI YATR 5 cut(s) 244, 279, 365, 460, 484
FaqI GGGAC 2 cut(s) 76, 215
FatI CATG 2 cut(s) 363, 458
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 1 cut(s) 171
FspBI CTAG 1 cut(s) 368
GlaI GCGC 2 cut(s) 241, 256
HaeIII GGCC 2 cut(s) 191, 213
HapII CCGG 2 cut(s) 94, 210
HgaI GACGC 3 cut(s) 58, 382, 430
HhaI GCGC 2 cut(s) 242, 257
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 2 cut(s) 367, 462
Hin6I GCGC 2 cut(s) 240, 255
HinP1I GCGC 2 cut(s) 240, 255
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 3 cut(s) 62, 122, 478
HpaII CCGG 2 cut(s) 94, 210
Hpy166II GTNNAC 3 cut(s) 99, 132, 172
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 2 cut(s) 100, 335
Hpy8I GTNNAC 3 cut(s) 99, 132, 172
Hpy99I CGWCG 4 cut(s) 23, 26, 74, 176
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 3 cut(s) 282, 294, 466
HpyCH4V TGCA 1 cut(s) 230
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 66, 263, 328, 399
HpyF3I CTNAG 1 cut(s) 425
HpySE526I ACGT 3 cut(s) 282, 294, 466
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 2 cut(s) 367, 462
HspAI GCGC 2 cut(s) 240, 255
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 5 cut(s) 54, 337, 360, 383, 387
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 7 cut(s) 51, 107, 169, 200, 206, 223, 227
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 368
MaeII ACGT 3 cut(s) 282, 294, 466
MaeIII GTNAC 4 cut(s) 283, 290, 295, 394
MalI GATC 5 cut(s) 56, 339, 362, 385, 389
MboI GATC 5 cut(s) 54, 337, 360, 383, 387
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 4 cut(s) 96, 128, 245, 484
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 110
MslI CAYNNNNRTG 2 cut(s) 345, 368
MspI CCGG 2 cut(s) 94, 210
MspR9I CCNGG 2 cut(s) 94, 215
Mva1269I GAATGC 1 cut(s) 162
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 2 cut(s) 257, 336
MwoI GCNNNNNNNGC 5 cut(s) 14, 66, 263, 328, 399
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 94
NdeII GATC 5 cut(s) 54, 337, 360, 383, 387
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 2 cut(s) 367, 462
NmuCI GTSAC 2 cut(s) 290, 394
NruI TCGCGA 1 cut(s) 336
PaeR7I CTCGAG 1 cut(s) 475
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 180
PctI GAATGC 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 122
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspPI GGNCC 1 cut(s) 190
PspXI VCTCGAGB 1 cut(s) 475
RruI TCGCGA 1 cut(s) 336
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 2 cut(s) 345, 368
SalI GTCGAC 1 cut(s) 170
Sau3AI GATC 5 cut(s) 54, 337, 360, 383, 387
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 2 cut(s) 94, 215
SfaNI GCATC 1 cut(s) 337
Sfr274I CTCGAG 1 cut(s) 475
SlaI CTCGAG 1 cut(s) 475
SmiMI CAYNNNNRTG 2 cut(s) 345, 368
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
SsiI CCGC 2 cut(s) 207, 257
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 2 cut(s) 92, 213
TaiI ACGT 3 cut(s) 285, 297, 469
TaqI TCGA 7 cut(s) 80, 120, 144, 171, 250, 386, 476
TfiI GAWTC 1 cut(s) 122
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 2 cut(s) 290, 394
Tsp45I GTSAC 2 cut(s) 290, 394
TspDTI ATGAA 1 cut(s) 447
TspRI CASTG 1 cut(s) 420
XhoI CTCGAG 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.