pycom10g24680

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
26481139 .. 26482530
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1392 bp
ATGGAGATATTCAGTGGCGGGTCCTATCCGGGACGTTTGGCTGACTTCGGACCCACAATCCCCCCCCCACTTTCGCCGATATTTCCATCGTCCTCCACAACTCGCCTTCTGGCGCGTGTCTCCACGTCACATCAATCCCCCTTCCGATTGAATCAAATCAAAACATCCGCAGCGAATGGCGACAGCGTGCGTGTGAGTTACTTAAAACCCCTAAAAAAAACCCAGCAACCCGTCTGGTACACCCAGCAGCACCCCTCTAAAAACCCAGCAGCTTCCCAGTCTCTTCATTTCTCACTTCCACAAGCAACAAATGAAACCGAAACCGAAAATCTAAACGCCGTGAAATCTGAAAGAAGAGGTTATATGGATCTCCGGCAGCGTCCGCAGCCAGCCCGCGGCGGACGCGGACCGATACGCGCGCCGCTGCCGCCGATGCAACAGGCCTACAACATAATCCCCATCCACGACCTCCTCGCAGACCACCCCTCCCTCCGCTATCCCGAGGTACGCGCCGCCGCCGCAGTCCTACGCGCCGTTGGGGACCTTCGTAAGCCGCCTTTTATCCCCTGGAATCCGTCCTACGACCTGATGGACTGGCTGGGACTATTCTTCGGGTTCCAAATCGACAACGTTCGGAACCAGCGGGAGCATTTGGTCCTCCACCTCGCCAACTCCCAAATGCGCCTCCAGCCTCCGCCGAACATAGTTGACTCGCTCGAGCCCGCCGTCCTCAGGCGGTTCCGCCGCAAATTGCTCTACAACTACACCTCCTGGTGCTCGTACCTCGGCCGGAAGTCGAGCGTCATCTTCGCCAGGCGCCGCGGCGGCGACGACCTCCGGCGCGAGCTGCTGTACGTGGCGCTGTTCCTGCTGATTTGGGGTGAGTCCGGTAACGTTCGATTCGTACCCGAATGTGTATGTTATATTTATCATCATATGGCTATGGAACTGAACAAAGTGCTTGATGAGTATACGGACCCAGATACGGGTAGGCCATTTCTGCCCTCGGTTTCCGGGGACTGCGGGTATCTGAAGAGTGTGGTGATGCCAATTTACCAGACGATTAAAGCCGAGGCGGAGAGCAGCAGAAATGGCACCGCTCCCCACTCGGCGTGGCGAAATTATGATGATATAAACGAGTATTTTTGGTCTAGGAGGTGTTTTAAGAAGCTCAAATGGCCGATTAAGTTCGATAGTAACTTTTTCGCTACTACTCCGAAGGATCGGAGGGTTGGGAAGACGGGGTTTGTGGAGCAGAGGTCGTTTTGGAATCTGTTTAGGAGCTTTGATAAGATCTGGGTGATGTTGATTTTGTTTTTACAGGCCGCTATTATTGTTGCATGGAAGGAGACAGAGTATCCGTGGCAGGCGTTGGATCGGAGGATTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0007623 GO:0008150 GO:0009555 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048229 GO:0048511 GO:0048856 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase

Protein Analysis

464

Amino Acids

53.44

Weight (kDa)

9.81

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 282 - 394 7.9e-44 1,3-beta-glucan synthase subunit FKS1, domain-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 816, 1094
AccBSI CCGCTC 1 cut(s) 1100
AccI GTMKAC 1 cut(s) 971
AccII CGCG 9 cut(s) 115, 396, 405, 417, 419, 510, 531, 822, 843
AclI AACGTT 2 cut(s) 630, 894
AclWI GGATC 3 cut(s) 375, 1230, 1383
AcoI YGGCCR 2 cut(s) 787, 1178
AcuI CTGAAG 1 cut(s) 1052
AcyI GRCGYC 1 cut(s) 817
AfaI GTAC 5 cut(s) 239, 507, 782, 854, 906
AfiI CCNNNNNNNGG 4 cut(s) 395, 732, 985, 986
AgsI TTSAA 1 cut(s) 151
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 3 cut(s) 566, 770, 812
AluBI AGCT 4 cut(s) 272, 847, 1171, 1284
AluI AGCT 4 cut(s) 272, 847, 1171, 1284
Alw21I GWGCWC 1 cut(s) 779
Alw26I GTCTC 3 cut(s) 124, 285, 1343
AlwI GGATC 3 cut(s) 375, 1230, 1383
Ama87I CYCGRG 2 cut(s) 500, 716
AoxI GGCC 5 cut(s) 441, 787, 992, 1178, 1323
ApeKI GCWGC 8 cut(s) 170, 247, 269, 376, 385, 424, 847, 1083
AspLEI GCGC 9 cut(s) 115, 419, 421, 512, 533, 684, 819, 843, 862
AspS9I GGNCC 6 cut(s) 21, 50, 407, 541, 655, 976
AsuC2I CCSGG 2 cut(s) 30, 1015
AsuHPI GGTGA 3 cut(s) 893, 1054, 1312
AvaI CYCGRG 2 cut(s) 500, 716
AvaII GGWCC 6 cut(s) 21, 50, 407, 541, 655, 976
AxyI CCTNAGG 1 cut(s) 731
BanI GGYRCC 2 cut(s) 816, 1094
BanII GRGCYC 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 1244
Bbv12I GWGCWC 1 cut(s) 779
BbvI GCAGC 8 cut(s) 182, 259, 281, 388, 397, 411, 834, 1095
BccI CCATC 3 cut(s) 94, 467, 583
BceAI ACGGC 3 cut(s) 323, 518, 710
BciT130I CCWGG 3 cut(s) 568, 772, 814
BciVI GTATCC 1 cut(s) 1368
BcnI CCSGG 2 cut(s) 30, 1015
BcoDI GTCTC 3 cut(s) 124, 285, 1343
BfaI CTAG 1 cut(s) 1152
BfoI RGCGCY 2 cut(s) 820, 863
BfuI GTATCC 1 cut(s) 1368
BglI GCCNNNNNGGC 1 cut(s) 825
BglII AGATCT 1 cut(s) 1293
Bme1390I CCNGG 5 cut(s) 30, 568, 772, 814, 1015
Bme18I GGWCC 6 cut(s) 21, 50, 407, 541, 655, 976
BmeT110I CYCGRG 2 cut(s) 500, 716
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 6 cut(s) 21, 50, 407, 541, 655, 976
BmiI GGNNCC 9 cut(s) 22, 52, 542, 617, 638, 740, 818, 978, 1096
BmrFI CCNGG 5 cut(s) 30, 568, 772, 814, 1015
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 2 cut(s) 423, 1035
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 1244
BpmI CTGGAG 1 cut(s) 671
BpuMI CCSGG 2 cut(s) 30, 1015
BsaAI YACGTR 1 cut(s) 856
BsaHI GRCGYC 1 cut(s) 817
BsaJI CCNNGG 9 cut(s) 394, 501, 566, 784, 820, 1005, 1014, 1071, 1361
BsaWI WCCGGW 1 cut(s) 887
BsaXI ACNNNNNCTCC 8 cut(s) 470, 500, 752, 782, 1244, 1274, 1340, 1370
Bsc4I CCNNNNNNNGG 4 cut(s) 395, 732, 985, 986
Bse1I ACTGG 2 cut(s) 277, 599
Bse21I CCTNAGG 1 cut(s) 731
BseBI CCWGG 3 cut(s) 568, 772, 814
BseDI CCNNGG 9 cut(s) 394, 501, 566, 784, 820, 1005, 1014, 1071, 1361
BseGI GGATG 2 cut(s) 164, 459
BseLI CCNNNNNNNGG 4 cut(s) 395, 732, 985, 986
BseMII CTCAG 1 cut(s) 745
BseNI ACTGG 2 cut(s) 277, 599
BsePI GCGCGC 1 cut(s) 417
BseRI GAGGAG 1 cut(s) 461
BseX3I CGGCCG 1 cut(s) 787
BseXI GCAGC 8 cut(s) 182, 259, 281, 388, 397, 411, 834, 1095
BseYI CCCAGC 4 cut(s) 222, 243, 265, 598
Bsh1236I CGCG 9 cut(s) 115, 396, 405, 417, 419, 510, 531, 822, 843
Bsh1285I CGRYCG 1 cut(s) 790
BshFI GGCC 5 cut(s) 443, 789, 994, 1180, 1325
BshNI GGYRCC 2 cut(s) 816, 1094
BsiEI CGRYCG 1 cut(s) 790
BsiHKAI GWGCWC 1 cut(s) 779
BsiHKCI CYCGRG 2 cut(s) 500, 716
BsiSI CCGG 6 cut(s) 29, 373, 790, 838, 888, 1014
BslFI GGGAC 4 cut(s) 45, 554, 615, 1031
BslI CCNNNNNNNGG 4 cut(s) 395, 732, 985, 986
BsmAI GTCTC 3 cut(s) 124, 285, 1343
BsmFI GGGAC 4 cut(s) 45, 554, 615, 1031
BsnI GGCC 5 cut(s) 443, 789, 994, 1180, 1325
BsoBI CYCGRG 2 cut(s) 500, 716
Bsp1286I GDGCHC 2 cut(s) 723, 779
Bsp143I GATC 4 cut(s) 367, 1222, 1293, 1375
BspANI GGCC 5 cut(s) 443, 789, 994, 1180, 1325
BspCNI CTCAG 1 cut(s) 744
BspFNI CGCG 9 cut(s) 115, 396, 405, 417, 419, 510, 531, 822, 843
BspLI GGNNCC 9 cut(s) 22, 52, 542, 617, 638, 740, 818, 978, 1096
BspPI GGATC 3 cut(s) 375, 1230, 1383
BspT107I GGYRCC 2 cut(s) 816, 1094
BsrBI CCGCTC 1 cut(s) 1100
BsrI ACTGG 2 cut(s) 277, 599
BssECI CCNNGG 9 cut(s) 394, 501, 566, 784, 820, 1005, 1014, 1071, 1361
BssHII GCGCGC 1 cut(s) 417
BssMI GATC 4 cut(s) 367, 1222, 1293, 1375
BssNAI GTATAC 1 cut(s) 972
BssNI GRCGYC 1 cut(s) 817
Bst1107I GTATAC 1 cut(s) 972
Bst2UI CCWGG 3 cut(s) 568, 772, 814
Bst6I CTCTTC 3 cut(s) 288, 349, 1028
BstACI GRCGYC 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 856
BstC8I GCNNGC 7 cut(s) 188, 390, 394, 419, 723, 845, 1368
BstDEI CTNAG 1 cut(s) 731
BstDSI CCRYGG 3 cut(s) 394, 820, 1361
BstF5I GGATG 2 cut(s) 164, 459
BstFNI CGCG 9 cut(s) 115, 396, 405, 417, 419, 510, 531, 822, 843
BstH2I RGCGCY 2 cut(s) 820, 863
BstHHI GCGC 9 cut(s) 115, 419, 421, 512, 533, 684, 819, 843, 862
BstKTI GATC 4 cut(s) 370, 1225, 1296, 1378
BstMAI GTCTC 3 cut(s) 124, 285, 1343
BstMBI GATC 4 cut(s) 367, 1222, 1293, 1375
BstMCI CGRYCG 1 cut(s) 790
BstNI CCWGG 3 cut(s) 568, 772, 814
BstSCI CCNGG 5 cut(s) 28, 566, 770, 812, 1013
BstUI CGCG 9 cut(s) 115, 396, 405, 417, 419, 510, 531, 822, 843
BstV1I GCAGC 8 cut(s) 182, 259, 281, 388, 397, 411, 834, 1095
BstV2I GAAGAC 1 cut(s) 1244
BstX2I RGATCY 2 cut(s) 367, 1293
BstYI RGATCY 2 cut(s) 367, 1293
BstZ17I GTATAC 1 cut(s) 972
BstZI CGGCCG 1 cut(s) 787
Bsu36I CCTNAGG 1 cut(s) 731
BsuI GTATCC 1 cut(s) 1368
BsuRI GGCC 5 cut(s) 443, 789, 994, 1180, 1325
BtgI CCRYGG 3 cut(s) 394, 820, 1361
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 2 cut(s) 164, 459
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 7 cut(s) 188, 390, 394, 419, 723, 845, 1368
CfoI GCGC 9 cut(s) 115, 419, 421, 512, 533, 684, 819, 843, 862
Cfr13I GGNCC 6 cut(s) 21, 50, 407, 541, 655, 976
Cfr42I CCGCGG 2 cut(s) 397, 823
CpoI CGGWCCG 1 cut(s) 407
CseI GACGC 3 cut(s) 368, 411, 790
Csp6I GTAC 5 cut(s) 238, 506, 781, 853, 905
CspI CGGWCCG 1 cut(s) 407
CviAII CATG 1 cut(s) 1341
CviQI GTAC 5 cut(s) 238, 506, 781, 853, 905
DdeI CTNAG 1 cut(s) 731
DinI GGCGCC 1 cut(s) 818
DpnI GATC 4 cut(s) 369, 1224, 1295, 1377
DpnII GATC 4 cut(s) 367, 1222, 1293, 1375
EaeI YGGCCR 2 cut(s) 787, 1178
EagI CGGCCG 1 cut(s) 787
Eam1104I CTCTTC 3 cut(s) 288, 349, 1028
EarI CTCTTC 3 cut(s) 288, 349, 1028
EciI GGCGGA 4 cut(s) 414, 684, 731, 1091
EclXI CGGCCG 1 cut(s) 787
Eco147I AGGCCT 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 723
Eco47I GGWCC 6 cut(s) 21, 50, 407, 541, 655, 976
Eco52I CGGCCG 1 cut(s) 787
Eco57I CTGAAG 1 cut(s) 1052
Eco81I CCTNAGG 1 cut(s) 731
Eco88I CYCGRG 2 cut(s) 500, 716
EcoO109I RGGNCCY 2 cut(s) 21, 541
EcoRII CCWGG 3 cut(s) 566, 770, 812
EcoT38I GRGCYC 1 cut(s) 723
EgeI GGCGCC 1 cut(s) 818
EheI GGCGCC 1 cut(s) 818
FaeI CATG 1 cut(s) 1344
FaqI GGGAC 4 cut(s) 45, 554, 615, 1031
FatI CATG 1 cut(s) 1340
FauI CCCGC 5 cut(s) 11, 401, 636, 730, 1016
FauNDI CATATG 1 cut(s) 936
FblI GTMKAC 1 cut(s) 971
FokI GGATG 2 cut(s) 151, 446
FriOI GRGCYC 1 cut(s) 723
FspBI CTAG 1 cut(s) 1152
GlaI GCGC 9 cut(s) 114, 418, 420, 511, 532, 683, 818, 842, 861
GsaI CCCAGC 4 cut(s) 226, 247, 269, 602
GsuI CTGGAG 1 cut(s) 671
HaeII RGCGCY 2 cut(s) 820, 863
HaeIII GGCC 5 cut(s) 443, 789, 994, 1180, 1325
HapII CCGG 6 cut(s) 29, 373, 790, 838, 888, 1014
HgaI GACGC 3 cut(s) 368, 411, 790
HhaI GCGC 9 cut(s) 115, 419, 421, 512, 533, 684, 819, 843, 862
Hin1I GRCGYC 1 cut(s) 817
Hin1II CATG 1 cut(s) 1344
Hin6I GCGC 9 cut(s) 113, 417, 419, 510, 531, 682, 817, 841, 860
HinP1I GCGC 9 cut(s) 113, 417, 419, 510, 531, 682, 817, 841, 860
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HinfI GANTC 7 cut(s) 151, 571, 710, 884, 900, 1270, 1384
HpaII CCGG 6 cut(s) 29, 373, 790, 838, 888, 1014
HphI GGTGA 3 cut(s) 893, 1054, 1312
Hpy166II GTNNAC 3 cut(s) 240, 709, 972
Hpy188I TCNGA 8 cut(s) 50, 146, 349, 636, 1032, 1218, 1227, 1380
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 3 cut(s) 240, 709, 972
Hpy99I CGWCG 1 cut(s) 833
HpyAV CCTTC 5 cut(s) 116, 151, 554, 1213, 1339
HpyCH4IV ACGT 5 cut(s) 34, 125, 630, 855, 894
HpyCH4V TGCA 2 cut(s) 436, 1340
HpyF3I CTNAG 1 cut(s) 731
HpySE526I ACGT 5 cut(s) 34, 125, 630, 855, 894
Hsp92I GRCGYC 1 cut(s) 817
Hsp92II CATG 1 cut(s) 1344
HspAI GCGC 9 cut(s) 113, 417, 419, 510, 531, 682, 817, 841, 860
KasI GGCGCC 1 cut(s) 816
KspI CCGCGG 2 cut(s) 397, 823
Kzo9I GATC 4 cut(s) 367, 1222, 1293, 1375
LmnI GCTCC 4 cut(s) 646, 1105, 1252, 1281
Lsp1109I GCAGC 8 cut(s) 182, 259, 281, 388, 397, 411, 834, 1095
LweI GCATC 2 cut(s) 423, 1035
MaeI CTAG 1 cut(s) 1152
MaeII ACGT 5 cut(s) 34, 125, 630, 855, 894
MaeIII GTNAC 4 cut(s) 126, 197, 890, 1196
MalI GATC 4 cut(s) 369, 1224, 1295, 1377
MbiI CCGCTC 1 cut(s) 1100
MboI GATC 4 cut(s) 367, 1222, 1293, 1375
MboII GAAGA 6 cut(s) 275, 366, 601, 799, 1045, 1249
MflI RGATCY 2 cut(s) 367, 1293
MhlI GDGCHC 2 cut(s) 723, 779
MluCI AATT 3 cut(s) 749, 1050, 1120
Mly113I GGCGCC 1 cut(s) 817
MlyI GAGTC 2 cut(s) 704, 893
MmeI TCCRAC 1 cut(s) 1353
MseI TTAA 4 cut(s) 203, 1065, 1164, 1185
MspA1I CMGCKG 4 cut(s) 396, 424, 643, 822
MspI CCGG 6 cut(s) 29, 373, 790, 838, 888, 1014
MspR9I CCNGG 5 cut(s) 30, 568, 772, 814, 1015
MvaI CCWGG 3 cut(s) 568, 772, 814
MvnI CGCG 9 cut(s) 115, 396, 405, 417, 419, 510, 531, 822, 843
NarI GGCGCC 1 cut(s) 817
NciI CCSGG 2 cut(s) 30, 1015
NdeI CATATG 1 cut(s) 936
NdeII GATC 4 cut(s) 367, 1222, 1293, 1375
NlaIII CATG 1 cut(s) 1344
NlaIV GGNNCC 9 cut(s) 22, 52, 542, 617, 638, 740, 818, 978, 1096
NmeAIII GCCGAG 3 cut(s) 765, 1088, 1096
NmuCI GTSAC 1 cut(s) 126
PaeR7I CTCGAG 1 cut(s) 716
PauI GCGCGC 1 cut(s) 417
PceI AGGCCT 1 cut(s) 443
PcsI WCGNNNNNNNCGW 3 cut(s) 723, 900, 1384
PfeI GAWTC 5 cut(s) 151, 571, 900, 1270, 1384
PfoI TCCNGGA 1 cut(s) 28
PleI GAGTC 2 cut(s) 704, 892
PluTI GGCGCC 1 cut(s) 820
PpsI GAGTC 2 cut(s) 704, 892
Ppu21I YACGTR 1 cut(s) 856
PpuMI RGGWCCY 2 cut(s) 21, 541
Psp1406I AACGTT 2 cut(s) 630, 894
Psp5II RGGWCCY 2 cut(s) 21, 541
Psp6I CCWGG 3 cut(s) 566, 770, 812
PspFI CCCAGC 4 cut(s) 222, 243, 265, 598
PspGI CCWGG 3 cut(s) 566, 770, 812
PspN4I GGNNCC 9 cut(s) 22, 52, 542, 617, 638, 740, 818, 978, 1096
PspPI GGNCC 6 cut(s) 21, 50, 407, 541, 655, 976
PspPPI RGGWCCY 2 cut(s) 21, 541
PspXI VCTCGAGB 1 cut(s) 716
PsuI RGATCY 2 cut(s) 367, 1293
PteI GCGCGC 1 cut(s) 417
RsaI GTAC 5 cut(s) 239, 507, 782, 854, 906
RsaNI GTAC 5 cut(s) 238, 506, 781, 853, 905
Rsr2I CGGWCCG 1 cut(s) 407
RsrII CGGWCCG 1 cut(s) 407
SacII CCGCGG 2 cut(s) 397, 823
SaqAI TTAA 4 cut(s) 203, 1065, 1164, 1185
Sau3AI GATC 4 cut(s) 367, 1222, 1293, 1375
Sau96I GGNCC 6 cut(s) 21, 50, 407, 541, 655, 976
SchI GAGTC 2 cut(s) 704, 893
ScrFI CCNGG 5 cut(s) 30, 568, 772, 814, 1015
SduI GDGCHC 2 cut(s) 723, 779
SfaNI GCATC 2 cut(s) 423, 1035
SfoI GGCGCC 1 cut(s) 818
Sfr274I CTCGAG 1 cut(s) 716
Sfr303I CCGCGG 2 cut(s) 397, 823
SgrBI CCGCGG 2 cut(s) 397, 823
SinI GGWCC 6 cut(s) 21, 50, 407, 541, 655, 976
SlaI CTCGAG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 3 cut(s) 749, 1050, 1120
SseBI AGGCCT 1 cut(s) 443
SspDI GGCGCC 1 cut(s) 816
SspMI CTAG 1 cut(s) 1152
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 5 cut(s) 28, 566, 770, 812, 1013
TaiI ACGT 5 cut(s) 37, 128, 633, 858, 897
TaqI TCGA 6 cut(s) 624, 717, 797, 898, 1191, 1387
TaqII GACCGA 1 cut(s) 424
TasI AATT 3 cut(s) 749, 1050, 1120
TfiI GAWTC 5 cut(s) 151, 571, 900, 1270, 1384
Tru1I TTAA 4 cut(s) 203, 1065, 1164, 1185
Tru9I TTAA 4 cut(s) 203, 1065, 1164, 1185
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 126
TseI GCWGC 8 cut(s) 170, 247, 269, 376, 385, 424, 847, 1083
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 2 cut(s) 275, 327
TspGWI ACGGA 3 cut(s) 564, 989, 1350
TspRI CASTG 1 cut(s) 19
VpaK11BI GGWCC 6 cut(s) 21, 50, 407, 541, 655, 976
XhoI CTCGAG 1 cut(s) 716
XmiI GTMKAC 1 cut(s) 971
XspI CTAG 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.