pycom10g25360

cellular response to nitrogen starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0028193
Physical Location & Seq
Forward (+)
27020206 .. 27021799
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGGCACAGAGCTCTTTTGCCGAATATGCTCGGGGAAACTTTGCGGTTTCAAGGACCATTATTGAGTTAGAGCCTTGGAAAGCCAGAGGATGGGGTGGGATATTTGAGAAGTTGCAGGAGACTGCCGTGAAGGAGAATGTGAAGGCTTGGATTGAGGCGAGGCCTGACATTCTTGACAAGGAGATTGCCATGAGAAAGATGGAGCCATATTTTAAGAGATGCAGACGTGAACTTCGTACTTGTCCAGAAGCTAGGGAATTGAGGCAAAAACGTCCAAACTTTGTACCAGTGATTGTGGAGAAGCATGGAAGGAGTGACATTCCTGACATTGAGTTGGTTAAATTCAATGTCCATGGTGAGCTGCCACTTGGAGAATTTGTTTATTATATTCGGGGACGGATCGGGTGGCTCAACGTAGACAACATAGACAAGCCTATATTTGTCTCTTTTAAGAACACAGAACCTCCTGCTGGTGCCTTGATGTCTGAAGTCGATGTGGAAAACAAGGGTGAAGATGGATTTCTTCACGTGACCTACAGTGGAGAAGGGAATGCCCCTGAATCCATCAATCATGAGCAAGAATGGAGAGAGAAGACAATGCCTGGACCTGACTTATTGTTGCGGAGAGGGAGAGGGCTTGCTGTGAAGACAATGCGGGAGCTCTCTCAGTCATTGATTCGACTACATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

231

Amino Acids

26.49

Weight (kDa)

7.78

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 82 - 182 3.9e-18 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028193)

Species Orthologous Gene IDs
malus_domestica MD10G1300700.v1.1
pyrus_communis pycom10g25360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 473
AccB7I CCANNNNNTGG 1 cut(s) 90
AccI GTMKAC 1 cut(s) 417
AciI CCGC 3 cut(s) 44, 622, 655
AclWI GGATC 1 cut(s) 407
AcsI RAATTY 2 cut(s) 341, 374
AcuI CTGAAG 1 cut(s) 507
AcvI CACGTG 1 cut(s) 529
AfaI GTAC 2 cut(s) 238, 285
AfiI CCNNNNNNNGG 2 cut(s) 90, 470
AgsI TTSAA 2 cut(s) 51, 346
AjiI CACGTC 1 cut(s) 227
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 4 cut(s) 12, 251, 361, 661
AluI AGCT 4 cut(s) 12, 251, 361, 661
Alw21I GWGCWC 2 cut(s) 14, 663
Alw26I GTCTC 2 cut(s) 113, 448
AlwI GGATC 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 361
ApoI RAATTY 2 cut(s) 341, 374
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
AspS9I GGNCC 2 cut(s) 54, 605
AsuHPI GGTGA 2 cut(s) 368, 521
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 2 cut(s) 54, 605
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 2 cut(s) 14, 663
BbrPI CACGTG 1 cut(s) 529
BbsI GAAGAC 2 cut(s) 599, 653
Bbv12I GWGCWC 2 cut(s) 14, 663
BbvI GCAGC 1 cut(s) 348
BccI CCATC 4 cut(s) 84, 193, 509, 572
BceAI ACGGC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 603
BcoDI GTCTC 2 cut(s) 113, 448
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 535
BisI GCNGC 1 cut(s) 362
BlsI GCNGC 1 cut(s) 363
Bme1390I CCNGG 1 cut(s) 603
Bme18I GGWCC 2 cut(s) 54, 605
BmeT110I CYCGRG 1 cut(s) 30
BmgBI CACGTC 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 54, 605
BmiI GGNNCC 2 cut(s) 204, 475
BmrFI CCNGG 1 cut(s) 603
BmsI GCATC 1 cut(s) 209
BpiI GAAGAC 2 cut(s) 599, 653
BsaAI YACGTR 1 cut(s) 529
BsaJI CCNNGG 2 cut(s) 74, 352
BsaXI ACNNNNNCTCC 4 cut(s) 363, 393, 448, 478
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 470
Bse1I ACTGG 1 cut(s) 287
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 2 cut(s) 74, 352
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 90, 470
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 348
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 2 cut(s) 14, 663
BsiHKCI CYCGRG 1 cut(s) 30
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 2 cut(s) 90, 470
BsmAI GTCTC 2 cut(s) 113, 448
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 1 cut(s) 163
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 14, 663
Bsp143I GATC 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 352
BspACI CCGC 3 cut(s) 44, 622, 655
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 1 cut(s) 679
BspHI TCATGA 1 cut(s) 571
BspLI GGNNCC 2 cut(s) 204, 475
BspPI GGATC 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 473
BsrI ACTGG 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 74, 352
BssMI GATC 1 cut(s) 399
BssT1I CCWWGG 2 cut(s) 74, 352
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 1 cut(s) 539
BstBAI YACGTR 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 2 cut(s) 666, 690
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 2 cut(s) 113, 448
BstMBI GATC 1 cut(s) 399
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 1 cut(s) 603
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 601
BstSFI CTRYAG 1 cut(s) 535
BstV1I GCAGC 1 cut(s) 348
BstV2I GAAGAC 2 cut(s) 599, 653
BsuRI GGCC 1 cut(s) 163
BtgI CCRYGG 1 cut(s) 352
BtrI CACGTC 1 cut(s) 227
BtsCI GGATG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 294, 544
Cac8I GCNNGC 1 cut(s) 639
CciI TCATGA 1 cut(s) 571
Cfr13I GGNCC 2 cut(s) 54, 605
Csp6I GTAC 2 cut(s) 237, 284
CviAII CATG 5 cut(s) 190, 305, 353, 572, 686
CviQI GTAC 2 cut(s) 237, 284
DdeI CTNAG 2 cut(s) 666, 690
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
Ecl136II GAGCTC 2 cut(s) 12, 661
Eco130I CCWWGG 2 cut(s) 74, 352
Eco147I AGGCCT 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 14, 663
Eco47I GGWCC 2 cut(s) 54, 605
Eco53kI GAGCTC 2 cut(s) 12, 661
Eco57I CTGAAG 1 cut(s) 507
Eco72I CACGTG 1 cut(s) 529
Eco88I CYCGRG 1 cut(s) 30
EcoICRI GAGCTC 2 cut(s) 12, 661
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 2 cut(s) 74, 352
EcoT38I GRGCYC 2 cut(s) 14, 663
ErhI CCWWGG 2 cut(s) 74, 352
FaeI CATG 5 cut(s) 193, 308, 356, 575, 689
FaqI GGGAC 1 cut(s) 408
FatI CATG 5 cut(s) 189, 304, 352, 571, 685
FauI CCCGC 1 cut(s) 648
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 1 cut(s) 362
FokI GGATG 1 cut(s) 102
FriOI GRGCYC 2 cut(s) 14, 663
Fsp4HI GCNGC 1 cut(s) 362
FspBI CTAG 1 cut(s) 252
GluI GCNGC 1 cut(s) 362
HaeIII GGCC 1 cut(s) 163
Hin1II CATG 5 cut(s) 193, 308, 356, 575, 689
HinfI GANTC 2 cut(s) 560, 676
HphI GGTGA 2 cut(s) 368, 521
Hpy166II GTNNAC 2 cut(s) 230, 418
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 4 cut(s) 173, 245, 323, 572
Hpy8I GTNNAC 2 cut(s) 230, 418
HpyAV CCTTC 4 cut(s) 124, 136, 303, 539
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 4 cut(s) 226, 271, 414, 528
HpyCH4V TGCA 2 cut(s) 115, 222
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 2 cut(s) 666, 690
HpySE526I ACGT 4 cut(s) 226, 271, 414, 528
Hsp92II CATG 5 cut(s) 193, 308, 356, 575, 689
Kzo9I GATC 1 cut(s) 399
LmnI GCTCC 2 cut(s) 202, 658
Lsp1109I GCAGC 1 cut(s) 348
LweI GCATC 1 cut(s) 209
MaeI CTAG 1 cut(s) 252
MaeII ACGT 4 cut(s) 226, 271, 414, 528
MaeIII GTNAC 2 cut(s) 314, 529
MalI GATC 1 cut(s) 401
MboI GATC 1 cut(s) 399
MboII GAAGA 4 cut(s) 515, 524, 604, 658
MhlI GDGCHC 2 cut(s) 14, 663
MluCI AATT 3 cut(s) 257, 341, 374
MnlI CCTC 7 cut(s) 80, 148, 153, 255, 474, 620, 626
MseI TTAA 3 cut(s) 213, 339, 450
MspR9I CCNGG 1 cut(s) 603
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 1 cut(s) 26
NcoI CCATGG 1 cut(s) 352
NdeII GATC 1 cut(s) 399
NlaIII CATG 5 cut(s) 193, 308, 356, 575, 689
NlaIV GGNNCC 2 cut(s) 204, 475
NmuCI GTSAC 2 cut(s) 314, 529
NspI RCATGY 1 cut(s) 689
PagI TCATGA 1 cut(s) 571
PceI AGGCCT 1 cut(s) 163
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 2 cut(s) 560, 676
PflMI CCANNNNNTGG 1 cut(s) 90
PkrI GCNGC 1 cut(s) 363
PmaCI CACGTG 1 cut(s) 529
PmlI CACGTG 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 529
Psp124BI GAGCTC 2 cut(s) 14, 663
Psp6I CCWGG 1 cut(s) 601
PspCI CACGTG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 2 cut(s) 204, 475
PspPI GGNCC 2 cut(s) 54, 605
RsaI GTAC 2 cut(s) 238, 285
RsaNI GTAC 2 cut(s) 237, 284
SacI GAGCTC 2 cut(s) 14, 663
SaqAI TTAA 3 cut(s) 213, 339, 450
SatI GCNGC 1 cut(s) 362
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 2 cut(s) 54, 605
ScrFI CCNGG 1 cut(s) 603
SduI GDGCHC 2 cut(s) 14, 663
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 535
SinI GGWCC 2 cut(s) 54, 605
Sse9I AATT 3 cut(s) 257, 341, 374
SseBI AGGCCT 1 cut(s) 163
SsiI CCGC 3 cut(s) 44, 622, 655
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 2 cut(s) 14, 663
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 1 cut(s) 601
StyI CCWWGG 2 cut(s) 74, 352
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 4 cut(s) 229, 274, 417, 531
TaqI TCGA 2 cut(s) 492, 679
TasI AATT 3 cut(s) 257, 341, 374
TfiI GAWTC 2 cut(s) 560, 676
Tru1I TTAA 3 cut(s) 213, 339, 450
Tru9I TTAA 3 cut(s) 213, 339, 450
TscAI CASTG 2 cut(s) 294, 544
TseFI GTSAC 2 cut(s) 314, 529
TseI GCWGC 1 cut(s) 361
Tsp45I GTSAC 2 cut(s) 314, 529
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 2 cut(s) 294, 544
Van91I CCANNNNNTGG 1 cut(s) 90
VpaK11BI GGWCC 2 cut(s) 54, 605
XapI RAATTY 2 cut(s) 341, 374
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 417
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.