pycom10g26950
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
N/A
Physical Location & Seq
Reverse (-)
28088340 .. 28089278
939 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGAAAATAGCCGAAGGAGCAGCAAAAGGGCTTGAGTACTTGCACGAAACTGCCAATCCGCCAGTGATTTACCGTGATTTGAAAGCTTCAAACATTTTGCTAGACGAAAAATTATGTCCAAAGCTCTCTGATTTTGGACTTGCTAAGTTAGGTCCAACCGGAGACAAGGACCATGTGACTACTAGAGTGATGGGGACTTATGGCTACTGTGCACCGGAATATGCTTCAACAGGACAGCTGACAATAATGTCCGATGTCTACAGCTTCGGAGTTGTGTTTCTCGAGTTAATCACAGGAAGGAGAGCTATTGATGATCAAAGACCGGTTGGGGAGCAGATCCTAGTCAGTTGGGCAAAGCCAAAGCTAAAGGACAGAAAGAAATACGCATCGATGATTGACCCGTTACTTCGAGGAAGATATCCGGCGAAGGGTCTGAACCAAGCTGTTGCAATTGCAGCAATGTGTCTGAACGAGGAAGCTGATTACCGGCCTGCGATGGGTGATGTTGTGACCGCTCTCGGGCACCTATCGTTGCCAGTATATGACAGAAAAAGTGCCAAGGGTGCATTGGGAAACAGAGGCGCTCACGTTGAGTCGGTAAGCAAAGGAAGTTTCGGAGGTGACAAATAG

Protein Analysis

210

Amino Acids

22.63

Weight (kDa)

8.85

Isoelectric Point (pI)

21.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 2 - 172 2e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 3 - 165 1.2e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 247
AccB1I GGYRCC 1 cut(s) 522
AccBSI CCGCTC 1 cut(s) 515
AccI GTMKAC 1 cut(s) 258
AciI CCGC 2 cut(s) 59, 513
AclWI GGATC 1 cut(s) 331
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 3 cut(s) 428, 497, 519
AgeI ACCGGT 1 cut(s) 322
AgsI TTSAA 3 cut(s) 82, 90, 228
AluBI AGCT 8 cut(s) 86, 124, 238, 264, 305, 364, 443, 479
AluI AGCT 8 cut(s) 86, 124, 238, 264, 305, 364, 443, 479
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 156
Alw44I GTGCAC 1 cut(s) 210
AlwI GGATC 1 cut(s) 331
Ama87I CYCGRG 2 cut(s) 281, 518
AoxI GGCC 1 cut(s) 488
ApaLI GTGCAC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 20, 455
AsiGI ACCGGT 1 cut(s) 322
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 2 cut(s) 152, 169
AsuHPI GGTGA 1 cut(s) 512
AvaI CYCGRG 2 cut(s) 281, 518
AvaII GGWCC 2 cut(s) 152, 169
BaeGI GKGCMC 2 cut(s) 214, 525
BanI GGYRCC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 32, 467
BccI CCATC 2 cut(s) 184, 490
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 3 cut(s) 101, 183, 341
BfmI CTRYAG 1 cut(s) 259
BfoI RGCGCY 1 cut(s) 585
BisI GCNGC 2 cut(s) 21, 456
BlsI GCNGC 2 cut(s) 22, 457
BmcAI AGTACT 1 cut(s) 38
Bme18I GGWCC 2 cut(s) 152, 169
BmeT110I CYCGRG 2 cut(s) 281, 518
BmgT120I GGNCC 2 cut(s) 152, 169
BmiI GGNNCC 1 cut(s) 524
BmsI GCATC 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 558
BsaWI WCCGGW 3 cut(s) 158, 214, 322
Bsc4I CCNNNNNNNGG 3 cut(s) 428, 497, 519
Bse118I RCCGGY 2 cut(s) 322, 486
Bse1I ACTGG 2 cut(s) 62, 536
Bse3DI GCAATG 1 cut(s) 465
BseCI ATCGAT 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 558
BseLI CCNNNNNNNGG 3 cut(s) 428, 497, 519
BseMI GCAATG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 62, 536
BseSI GKGCMC 2 cut(s) 214, 525
BseXI GCAGC 2 cut(s) 32, 467
BshFI GGCC 1 cut(s) 490
BshNI GGYRCC 1 cut(s) 522
BshTI ACCGGT 1 cut(s) 322
BshVI ATCGAT 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 2 cut(s) 281, 518
BsiSI CCGG 5 cut(s) 159, 215, 323, 422, 487
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 3 cut(s) 428, 497, 519
BsmAI GTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 1 cut(s) 490
BsoBI CYCGRG 2 cut(s) 281, 518
Bsp1286I GDGCHC 2 cut(s) 214, 525
Bsp143I GATC 2 cut(s) 313, 336
BspACI CCGC 2 cut(s) 59, 513
BspANI GGCC 1 cut(s) 490
BspDI ATCGAT 1 cut(s) 389
BspLI GGNNCC 1 cut(s) 524
BspPI GGATC 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 522
BsrBI CCGCTC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 465
BsrFI RCCGGY 2 cut(s) 322, 486
BsrI ACTGG 2 cut(s) 62, 536
BssAI RCCGGY 2 cut(s) 322, 486
BssECI CCNNGG 1 cut(s) 558
BssMI GATC 2 cut(s) 313, 336
BssT1I CCWWGG 1 cut(s) 558
Bst4CI ACNGT 2 cut(s) 74, 209
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 2 cut(s) 316, 339
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 313, 336
BstMWI GCNNNNNNNGC 3 cut(s) 17, 455, 563
BstSFI CTRYAG 1 cut(s) 259
BstSLI GKGCMC 2 cut(s) 214, 525
BstV1I GCAGC 2 cut(s) 32, 467
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
Bsu15I ATCGAT 1 cut(s) 389
BsuRI GGCC 1 cut(s) 490
BsuTUI ATCGAT 1 cut(s) 389
BtgZI GCGATG 1 cut(s) 509
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 492
CfoI GCGC 1 cut(s) 584
Cfr10I RCCGGY 2 cut(s) 322, 486
Cfr13I GGNCC 2 cut(s) 152, 169
ClaI ATCGAT 1 cut(s) 389
Csp6I GTAC 1 cut(s) 37
CspAI ACCGGT 1 cut(s) 322
CviAII CATG 1 cut(s) 173
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 1 cut(s) 144
DpnI GATC 2 cut(s) 315, 338
DpnII GATC 2 cut(s) 313, 336
DrdI GACNNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 247
EciI GGCGGA 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 558
Eco32I GATATC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 152, 169
Eco88I CYCGRG 2 cut(s) 281, 518
EcoRV GATATC 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 1 cut(s) 176
FaiI YATR 6 cut(s) 115, 174, 201, 222, 541, 543
FaqI GGGAC 1 cut(s) 208
FatI CATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 21, 456
Fsp4HI GCNGC 2 cut(s) 21, 456
FspBI CTAG 3 cut(s) 101, 183, 341
GlaI GCGC 1 cut(s) 583
GluI GCNGC 2 cut(s) 21, 456
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 1 cut(s) 490
HapII CCGG 5 cut(s) 159, 215, 323, 422, 487
HhaI GCGC 1 cut(s) 584
Hin1II CATG 1 cut(s) 176
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 1 cut(s) 593
HpaII CCGG 5 cut(s) 159, 215, 323, 422, 487
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 2 cut(s) 212, 259
Hpy188I TCNGA 6 cut(s) 130, 253, 269, 435, 468, 617
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 2 cut(s) 212, 259
HpyAV CCTTC 3 cut(s) 8, 291, 421
HpyCH4III ACNGT 2 cut(s) 74, 209
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 5 cut(s) 43, 212, 449, 455, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 455, 563
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 1 cut(s) 176
HspAI GCGC 1 cut(s) 582
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 2 cut(s) 313, 336
LmnI GCTCC 2 cut(s) 17, 331
Lsp1109I GCAGC 2 cut(s) 32, 467
LweI GCATC 1 cut(s) 395
MaeI CTAG 3 cut(s) 101, 183, 341
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 4 cut(s) 175, 402, 508, 620
MalI GATC 2 cut(s) 315, 338
MbiI CCGCTC 1 cut(s) 515
MboI GATC 2 cut(s) 313, 336
MboII GAAGA 1 cut(s) 426
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 2 cut(s) 214, 525
MluCI AATT 2 cut(s) 110, 450
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 4 cut(s) 404, 466, 572, 611
MseI TTAA 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 5 cut(s) 159, 215, 323, 422, 487
MunI CAATTG 1 cut(s) 450
MwoI GCNNNNNNNGC 3 cut(s) 17, 455, 563
NdeII GATC 2 cut(s) 313, 336
NlaIII CATG 1 cut(s) 176
NlaIV GGNNCC 1 cut(s) 524
NmuCI GTSAC 3 cut(s) 175, 508, 620
PaeR7I CTCGAG 1 cut(s) 281
PinAI ACCGGT 1 cut(s) 322
PkrI GCNGC 2 cut(s) 22, 457
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PspN4I GGNNCC 1 cut(s) 524
PspPI GGNCC 2 cut(s) 152, 169
PsuI RGATCY 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 2 cut(s) 21, 456
Sau3AI GATC 2 cut(s) 313, 336
Sau96I GGNCC 2 cut(s) 152, 169
ScaI AGTACT 1 cut(s) 38
SchI GAGTC 1 cut(s) 602
SduI GDGCHC 2 cut(s) 214, 525
SfaNI GCATC 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 259
Sfr274I CTCGAG 1 cut(s) 281
SinI GGWCC 2 cut(s) 152, 169
SlaI CTCGAG 1 cut(s) 281
SmlI CTYRAG 2 cut(s) 32, 281
SmoI CTYRAG 2 cut(s) 32, 281
Sse9I AATT 2 cut(s) 110, 450
SsiI CCGC 2 cut(s) 59, 513
SspMI CTAG 3 cut(s) 101, 183, 341
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 2 cut(s) 74, 209
TaiI ACGT 1 cut(s) 591
TaqI TCGA 3 cut(s) 282, 389, 409
TasI AATT 2 cut(s) 110, 450
TatI WGTACW 1 cut(s) 36
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 1 cut(s) 69
TseFI GTSAC 3 cut(s) 175, 508, 620
TseI GCWGC 2 cut(s) 20, 455
Tsp45I GTSAC 3 cut(s) 175, 508, 620
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 69
VneI GTGCAC 1 cut(s) 210
VpaK11BI GGWCC 2 cut(s) 152, 169
XcmI CCANNNNNNNNNTGG 1 cut(s) 565
XhoI CTCGAG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 3 cut(s) 101, 183, 341
ZrmI AGTACT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.