pycom111g00160

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
N/A
Physical Location & Seq
Reverse (-)
146918 .. 148956
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 849 bp
ATGCCATTGCCTTTGACTGTCTTTGTAATTCTTGCTAACTGGAACCTGGTTCTCAAATATTCTCTAGAGGAAAGTTCTGATCGCGCTGAGTTTCTCAAGCTCTGTAAGAGAGTCGAGTACACAATTCGGGCTTGGTATCTCCTTCAATTTGAGGATTTGATGCTTGACAATAAACTTTTGAGGAAATACTTTAACACGCATCCTTGCCCAAACATTCCAGATTTTGCTGATAAGTACGTCATCTTCCGACGCGGCATTGGACTTGATAAGACTTCAGATTACTTTATCATGGAGAAAGTGGACATGATCATTGCACGTTGCTGGAAATATCTTTTAAGACTAACCAAGACAGAAAATTTCTTTTCAAAAAAGAAAGAGGCACAGAGCAAGATAGATCCAAAGAAGGATGATAATATTCGCCTCCCTGACCAAGATCCAGAGGACTTGTTTGTTGAACGAATCCGTATTGAAAAAATGGAACTGAGCATTCAAAATTTGCTGTGCAAGAACACAATCCAAGAGCCAACTTTTGATAGGATTATTGTTGTTTACAGGCGAGCAAGTACCGAAGATAATGAAGAGCGGGGAATATATGTTAAACATTTTAAAAACATTCCAATGGCCGATATGGAGATTGTTCTTGTGAGTATCTCTATCTATCACTGTCTCCCTCAGGGCATTTGTACACCTGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGCTCACGTTCGTTGGCTCTGCCGTAGTAGGGCTGGTCAGTTTCTTGATAGCAGCAATGAAGTTTGTACTGCTGCTGCACAGTAATCTTGTCATTTTTCTTTTATATTGTGAAAATGCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

283

Amino Acids

33.03

Weight (kDa)

7.54

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 177 - 214 9.6e-06 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 583
AccII CGCG 2 cut(s) 84, 252
AciI CCGC 2 cut(s) 252, 583
AclWI GGATC 2 cut(s) 389, 428
AcoI YGGCCR 1 cut(s) 621
AcsI RAATTY 2 cut(s) 355, 493
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 5 cut(s) 119, 236, 565, 685, 792
AfiI CCNNNNNNNGG 1 cut(s) 753
AgsI TTSAA 5 cut(s) 146, 366, 455, 470, 491
AjnI CCWGG 2 cut(s) 45, 703
AluBI AGCT 2 cut(s) 100, 845
AluI AGCT 2 cut(s) 100, 845
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 2 cut(s) 389, 428
AoxI GGCC 1 cut(s) 621
ApeKI GCWGC 4 cut(s) 776, 796, 799, 842
ApoI RAATTY 2 cut(s) 355, 493
AspLEI GCGC 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 672
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BbvI GCAGC 3 cut(s) 783, 786, 788
BceAI ACGGC 1 cut(s) 731
BciT130I CCWGG 2 cut(s) 47, 705
BclI TGATCA 1 cut(s) 306
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 1 cut(s) 65
BisI GCNGC 5 cut(s) 253, 777, 797, 800, 843
BlsI GCNGC 5 cut(s) 254, 778, 798, 801, 844
Bme1390I CCNGG 2 cut(s) 47, 705
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 2 cut(s) 47, 705
BmsI GCATC 2 cut(s) 150, 208
BpuEI CTTGAG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 703
Bsc4I CCNNNNNNNGG 1 cut(s) 753
Bse1I ACTGG 2 cut(s) 44, 728
Bse21I CCTNAGG 1 cut(s) 672
Bse3DI GCAATG 3 cut(s) 5, 309, 786
BseBI CCWGG 2 cut(s) 47, 705
BseDI CCNNGG 1 cut(s) 703
BseGI GGATG 2 cut(s) 199, 412
BseLI CCNNNNNNNGG 1 cut(s) 753
BseMI GCAATG 3 cut(s) 5, 309, 786
BseMII CTCAG 4 cut(s) 78, 473, 681, 686
BseNI ACTGG 2 cut(s) 44, 728
BseXI GCAGC 3 cut(s) 783, 786, 788
BsgI GTGCAG 1 cut(s) 785
Bsh1236I CGCG 2 cut(s) 84, 252
BshFI GGCC 1 cut(s) 623
BslI CCNNNNNNNGG 1 cut(s) 753
BsmAI GTCTC 1 cut(s) 671
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 1 cut(s) 623
Bsp1407I TGTACA 1 cut(s) 683
Bsp143I GATC 4 cut(s) 79, 306, 394, 433
BspACI CCGC 2 cut(s) 252, 583
BspANI GGCC 1 cut(s) 623
BspCNI CTCAG 4 cut(s) 79, 474, 682, 685
BspFNI CGCG 2 cut(s) 84, 252
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 2 cut(s) 389, 428
BspQI GCTCTTC 1 cut(s) 573
BsrBI CCGCTC 1 cut(s) 583
BsrDI GCAATG 3 cut(s) 5, 309, 786
BsrGI TGTACA 1 cut(s) 683
BsrI ACTGG 2 cut(s) 44, 728
BssECI CCNNGG 1 cut(s) 703
BssMI GATC 4 cut(s) 79, 306, 394, 433
Bst2UI CCWGG 2 cut(s) 47, 705
Bst4CI ACNGT 3 cut(s) 19, 665, 806
Bst6I CTCTTC 1 cut(s) 573
BstAUI TGTACA 1 cut(s) 683
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 4 cut(s) 87, 482, 672, 690
BstF5I GGATG 2 cut(s) 199, 412
BstFNI CGCG 2 cut(s) 84, 252
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 4 cut(s) 82, 309, 397, 436
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 4 cut(s) 79, 306, 394, 433
BstNI CCWGG 2 cut(s) 47, 705
BstSCI CCNGG 2 cut(s) 45, 703
BstUI CGCG 2 cut(s) 84, 252
BstV1I GCAGC 3 cut(s) 783, 786, 788
BstX2I RGATCY 2 cut(s) 394, 433
BstXI CCANNNNNNTGG 1 cut(s) 723
BstYI RGATCY 2 cut(s) 394, 433
Bsu36I CCTNAGG 1 cut(s) 672
BsuRI GGCC 1 cut(s) 623
BtsCI GGATG 2 cut(s) 199, 412
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 1 cut(s) 558
CfoI GCGC 1 cut(s) 86
CseI GACGC 1 cut(s) 258
CsiI ACCWGGT 1 cut(s) 45
Csp6I GTAC 5 cut(s) 118, 235, 564, 684, 791
CviAII CATG 2 cut(s) 289, 304
CviJI RGCY 8 cut(s) 100, 131, 523, 623, 727, 741, 757, 845
CviKI_1 RGCY 8 cut(s) 100, 131, 523, 623, 727, 741, 757, 845
CviQI GTAC 5 cut(s) 118, 235, 564, 684, 791
DdeI CTNAG 4 cut(s) 87, 482, 672, 690
DpnI GATC 4 cut(s) 81, 308, 396, 435
DpnII GATC 4 cut(s) 79, 306, 394, 433
DraI TTTAAA 1 cut(s) 607
EaeI YGGCCR 1 cut(s) 621
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
Eco57I CTGAAG 1 cut(s) 258
Eco81I CCTNAGG 1 cut(s) 672
EcoRII CCWGG 2 cut(s) 45, 703
FaeI CATG 2 cut(s) 292, 307
FaiI YATR 6 cut(s) 290, 305, 592, 594, 629, 829
FatI CATG 2 cut(s) 288, 303
FauI CCCGC 1 cut(s) 576
FbaI TGATCA 1 cut(s) 306
Fnu4HI GCNGC 5 cut(s) 253, 777, 797, 800, 843
FokI GGATG 2 cut(s) 186, 419
Fsp4HI GCNGC 5 cut(s) 253, 777, 797, 800, 843
FspBI CTAG 1 cut(s) 65
GlaI GCGC 1 cut(s) 85
GluI GCNGC 5 cut(s) 253, 777, 797, 800, 843
HaeIII GGCC 1 cut(s) 623
HgaI GACGC 1 cut(s) 258
HhaI GCGC 1 cut(s) 86
Hin1II CATG 2 cut(s) 292, 307
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 2 cut(s) 111, 459
Hpy166II GTNNAC 4 cut(s) 120, 301, 550, 686
Hpy188I TCNGA 3 cut(s) 79, 248, 277
Hpy188III TCNNGA 4 cut(s) 65, 218, 437, 769
Hpy8I GTNNAC 4 cut(s) 120, 301, 550, 686
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 2 cut(s) 152, 397
HpyCH4III ACNGT 3 cut(s) 19, 665, 806
HpyCH4IV ACGT 3 cut(s) 237, 316, 731
HpyCH4V TGCA 4 cut(s) 314, 504, 802, 842
HpyF3I CTNAG 4 cut(s) 87, 482, 672, 690
HpySE526I ACGT 3 cut(s) 237, 316, 731
Hsp92II CATG 2 cut(s) 292, 307
HspAI GCGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 306
Kzo9I GATC 4 cut(s) 79, 306, 394, 433
LguI GCTCTTC 1 cut(s) 573
Lsp1109I GCAGC 3 cut(s) 783, 786, 788
LweI GCATC 2 cut(s) 150, 208
MabI ACCWGGT 1 cut(s) 45
MaeI CTAG 1 cut(s) 65
MaeII ACGT 3 cut(s) 237, 316, 731
MalI GATC 4 cut(s) 81, 308, 396, 435
MbiI CCGCTC 1 cut(s) 583
MboI GATC 4 cut(s) 79, 306, 394, 433
MboII GAAGA 5 cut(s) 235, 581, 590, 706, 709
MflI RGATCY 2 cut(s) 394, 433
MluCI AATT 5 cut(s) 27, 123, 146, 355, 493
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 7 cut(s) 61, 145, 174, 370, 431, 433, 681
MseI TTAA 6 cut(s) 192, 335, 597, 606, 710, 847
MslI CAYNNNNRTG 1 cut(s) 617
MspR9I CCNGG 2 cut(s) 47, 705
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 2 cut(s) 47, 705
MvnI CGCG 2 cut(s) 84, 252
NdeII GATC 4 cut(s) 79, 306, 394, 433
NlaIII CATG 2 cut(s) 292, 307
NlaIV GGNNCC 1 cut(s) 44
PciSI GCTCTTC 1 cut(s) 573
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 459
PkrI GCNGC 5 cut(s) 254, 778, 798, 801, 844
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
Psp6I CCWGG 2 cut(s) 45, 703
PspGI CCWGG 2 cut(s) 45, 703
PspN4I GGNNCC 1 cut(s) 44
PsuI RGATCY 2 cut(s) 394, 433
RsaI GTAC 5 cut(s) 119, 236, 565, 685, 792
RsaNI GTAC 5 cut(s) 118, 235, 564, 684, 791
RseI CAYNNNNRTG 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 6 cut(s) 192, 335, 597, 606, 710, 847
SatI GCNGC 5 cut(s) 253, 777, 797, 800, 843
Sau3AI GATC 4 cut(s) 79, 306, 394, 433
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 47, 705
SetI ASST 7 cut(s) 48, 102, 240, 319, 691, 734, 847
SexAI ACCWGGT 1 cut(s) 45
SfaNI GCATC 2 cut(s) 150, 208
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 5 cut(s) 27, 123, 146, 355, 493
SsiI CCGC 2 cut(s) 252, 583
SspI AATATT 2 cut(s) 59, 415
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 45, 703
TaaI ACNGT 3 cut(s) 19, 665, 806
TaiI ACGT 3 cut(s) 240, 319, 734
TaqI TCGA 1 cut(s) 114
TasI AATT 5 cut(s) 27, 123, 146, 355, 493
TatI WGTACW 3 cut(s) 117, 683, 790
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 1 cut(s) 459
Tru1I TTAA 6 cut(s) 192, 335, 597, 606, 710, 847
Tru9I TTAA 6 cut(s) 192, 335, 597, 606, 710, 847
TscAI CASTG 1 cut(s) 668
TseI GCWGC 4 cut(s) 776, 796, 799, 842
TspDTI ATGAA 2 cut(s) 591, 797
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 1 cut(s) 668
XapI RAATTY 2 cut(s) 355, 493
XbaI TCTAGA 1 cut(s) 64
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.