pycom111g00400

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
N/A
Physical Location & Seq
Forward (+)
288170 .. 290035
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 990 bp
ATGGGGTGCGGCTCTTGTGTTTATGCTATTGGAGGAAAGAAGAAGTTGACTGTCCCTGAAGTCGCGGTCTTCGTTCCGTCTACGCGAATCCCGGCTCAGTCTGATGTTCAAAAGCGTCTTAGAGGCCTCATTCCAATAGATCTTGTTGACAGACTGCCATCGCTTCGCAATCAGATTGTCTTAGTTGCAGAGGACACCGGTATGCCCTACTCTCTTTTTATATTAAACCTCTGGGATAACCTGGAGTATTTGTCATTAATTTGTATATATGGGATAACCTGGAGTATTTCATGTGTGGCAATCTCCTGGTTTAAAGTACTATCTGTTGTGCACCTGATGGCTATGCTTACATTGTCGGAGGCTGACACCTTGATGATCCCATGCAACCATTCTGGTTCGACTATAGGGACAGTTTCTTCAGATTCCAAGAGAGATGCTATCGACTTATTGCTCAAGGCATCTGGCCACTTGGAATTCTGTGTCCGGGATGTCCTGGTTCGCATACCACCCGAAATCAAGTATGAAAAATTTGCCCAAAATTTTGCAGGATGGTTATTGGAGGCCATTTCTTATCAAACTCTAGGCCAGCCAGATCGGGCCTATCACTGCTTGTCAGGATGTGACATGAATAACGGGTGTGAGAAGAAGCATATCTGGTTCATCAATTGGAAATTTCTTGCGTCAAAGGCTGCATCGTATTACTATCACGGTCTGATCCTTGACAAGGGCAATGAACCGTCCTGCCACGTTAGCGCTCTATGCTGTTTTCTTGCGGCGGAAGAACTTCTATCAGAGAGCAAGAAGGCTTGCTTAACCTTTTGCCTTGCAGCTCCAGTTACCAGGGCTCCTCCACTCTGGGGAGCTATGAGGCATTTGCATCAGAAAATTCCTAAAGTTGCAACAGGGAAACCTCAGATGTATGGCTACCTTTTAGAACAAGAAAAGGTTCTACAAACATTGCCGACCTGCCAGAATTTCAACTGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

330

Amino Acids

36.66

Weight (kDa)

8.09

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 974
AccI GTMKAC 1 cut(s) 80
AccII CGCG 2 cut(s) 65, 85
AciI CCGC 4 cut(s) 9, 65, 773, 776
AclWI GGATC 2 cut(s) 370, 709
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 6 cut(s) 473, 527, 538, 671, 885, 973
AcuI CTGAAG 2 cut(s) 78, 402
AfaI GTAC 1 cut(s) 318
AfeI AGCGCT 1 cut(s) 754
AfiI CCNNNNNNNGG 2 cut(s) 724, 857
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 2 cut(s) 110, 979
AjnI CCWGG 5 cut(s) 240, 278, 305, 492, 839
AluBI AGCT 2 cut(s) 830, 863
AluI AGCT 2 cut(s) 830, 863
Alw21I GWGCWC 1 cut(s) 333
Alw44I GTGCAC 1 cut(s) 329
AlwI GGATC 2 cut(s) 370, 709
Aor51HI AGCGCT 1 cut(s) 754
AoxI GGCC 5 cut(s) 124, 463, 561, 583, 597
ApaLI GTGCAC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 689, 827
ApoI RAATTY 6 cut(s) 473, 527, 538, 671, 885, 973
AseI ATTAAT 1 cut(s) 257
AsiGI ACCGGT 1 cut(s) 197
Asp700I GAANNNNTTC 2 cut(s) 783, 945
AspLEI GCGC 1 cut(s) 755
AspS9I GGNCC 1 cut(s) 597
AsuC2I CCSGG 2 cut(s) 92, 485
BaeGI GKGCMC 1 cut(s) 333
BalI TGGCCA 1 cut(s) 465
BanII GRGCYC 1 cut(s) 847
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 2 cut(s) 676, 839
BccI CCATC 3 cut(s) 166, 331, 543
BciT130I CCWGG 5 cut(s) 242, 280, 307, 494, 841
BcnI CCSGG 2 cut(s) 92, 485
BfaI CTAG 1 cut(s) 581
BfmI CTRYAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 756
BfuAI ACCTGC 1 cut(s) 974
BglII AGATCT 1 cut(s) 139
BisI GCNGC 4 cut(s) 10, 690, 774, 828
BlsI GCNGC 4 cut(s) 11, 691, 775, 829
BmcAI AGTACT 1 cut(s) 318
Bme1390I CCNGG 7 cut(s) 92, 242, 280, 307, 485, 494, 841
BmgT120I GGNCC 1 cut(s) 597
BmiI GGNNCC 1 cut(s) 846
BmrFI CCNGG 7 cut(s) 92, 242, 280, 307, 485, 494, 841
BmsI GCATC 4 cut(s) 424, 467, 701, 886
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 3 cut(s) 263, 301, 816
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 2 cut(s) 92, 485
BsaJI CCNNGG 1 cut(s) 840
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 829, 859
Bsc4I CCNNNNNNNGG 2 cut(s) 724, 857
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 833
Bse3DI GCAATG 2 cut(s) 736, 956
BseBI CCWGG 5 cut(s) 242, 280, 307, 494, 841
BseDI CCNNGG 1 cut(s) 840
BseGI GGATG 3 cut(s) 493, 554, 623
BseLI CCNNNNNNNGG 2 cut(s) 724, 857
BseMI GCAATG 2 cut(s) 736, 956
BseMII CTCAG 2 cut(s) 110, 926
BseNI ACTGG 1 cut(s) 833
BseRI GAGGAG 1 cut(s) 837
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 2 cut(s) 676, 839
Bsh1236I CGCG 2 cut(s) 65, 85
BshFI GGCC 5 cut(s) 126, 465, 563, 585, 599
BshTI ACCGGT 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 3 cut(s) 92, 198, 484
BslFI GGGAC 2 cut(s) 38, 421
BslI CCNNNNNNNGG 2 cut(s) 724, 857
BsmFI GGGAC 2 cut(s) 38, 421
BsnI GGCC 5 cut(s) 126, 465, 563, 585, 599
Bsp1286I GDGCHC 2 cut(s) 333, 847
Bsp143I GATC 4 cut(s) 139, 375, 592, 714
BspACI CCGC 4 cut(s) 9, 65, 773, 776
BspANI GGCC 5 cut(s) 126, 465, 563, 585, 599
BspCNI CTCAG 2 cut(s) 109, 925
BspFNI CGCG 2 cut(s) 65, 85
BspLI GGNNCC 1 cut(s) 846
BspMI ACCTGC 1 cut(s) 974
BspPI GGATC 2 cut(s) 370, 709
BsrDI GCAATG 2 cut(s) 736, 956
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 1 cut(s) 833
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 1 cut(s) 840
BssMI GATC 4 cut(s) 139, 375, 592, 714
Bst2UI CCWGG 5 cut(s) 242, 280, 307, 494, 841
Bst4CI ACNGT 5 cut(s) 52, 412, 710, 738, 983
BstC8I GCNNGC 2 cut(s) 587, 808
BstDEI CTNAG 4 cut(s) 96, 119, 181, 912
BstENI CCTNNNNNAGG 1 cut(s) 722
BstF5I GGATG 3 cut(s) 493, 554, 623
BstFNI CGCG 2 cut(s) 65, 85
BstH2I RGCGCY 1 cut(s) 756
BstHHI GCGC 1 cut(s) 755
BstKTI GATC 4 cut(s) 142, 378, 595, 717
BstMBI GATC 4 cut(s) 139, 375, 592, 714
BstMWI GCNNNNNNNGC 3 cut(s) 686, 750, 759
BstNI CCWGG 5 cut(s) 242, 280, 307, 494, 841
BstSCI CCNGG 7 cut(s) 90, 240, 278, 305, 483, 492, 839
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 333
BstUI CGCG 2 cut(s) 65, 85
BstV1I GCAGC 2 cut(s) 676, 839
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 5 cut(s) 126, 465, 563, 585, 599
BtgZI GCGATG 1 cut(s) 144
BtsCI GGATG 3 cut(s) 493, 554, 623
BtsI GCAGTG 1 cut(s) 604
BtsIMutI CAGTG 1 cut(s) 604
BveI ACCTGC 1 cut(s) 974
Cac8I GCNNGC 2 cut(s) 587, 808
CfoI GCGC 1 cut(s) 755
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 597
CseI GACGC 2 cut(s) 104, 669
Csp6I GTAC 1 cut(s) 317
CspAI ACCGGT 1 cut(s) 197
CviAII CATG 3 cut(s) 291, 381, 625
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 4 cut(s) 96, 119, 181, 912
DpnI GATC 4 cut(s) 141, 377, 594, 716
DpnII GATC 4 cut(s) 139, 375, 592, 714
DraI TTTAAA 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 463
EciI GGCGGA 1 cut(s) 791
Eco147I AGGCCT 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 847
Eco47III AGCGCT 1 cut(s) 754
Eco57I CTGAAG 2 cut(s) 78, 402
EcoNI CCTNNNNNAGG 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 473
EcoRII CCWGG 5 cut(s) 240, 278, 305, 492, 839
EcoT38I GRGCYC 1 cut(s) 847
FaeI CATG 3 cut(s) 294, 384, 628
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 2 cut(s) 38, 421
FatI CATG 3 cut(s) 290, 380, 624
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 4 cut(s) 10, 690, 774, 828
FokI GGATG 3 cut(s) 500, 561, 630
FriOI GRGCYC 1 cut(s) 847
Fsp4HI GCNGC 4 cut(s) 10, 690, 774, 828
FspBI CTAG 1 cut(s) 581
GlaI GCGC 1 cut(s) 754
GluI GCNGC 4 cut(s) 10, 690, 774, 828
GsuI CTGGAG 3 cut(s) 263, 301, 816
HaeII RGCGCY 1 cut(s) 756
HaeIII GGCC 5 cut(s) 126, 465, 563, 585, 599
HapII CCGG 3 cut(s) 92, 198, 484
HgaI GACGC 2 cut(s) 104, 669
HhaI GCGC 1 cut(s) 755
Hin1II CATG 3 cut(s) 294, 384, 628
Hin6I GCGC 1 cut(s) 753
HinP1I GCGC 1 cut(s) 753
HincII GTYRAC 2 cut(s) 48, 148
HindII GTYRAC 2 cut(s) 48, 148
HinfI GANTC 2 cut(s) 87, 422
HpaII CCGG 3 cut(s) 92, 198, 484
Hpy166II GTNNAC 4 cut(s) 48, 81, 148, 331
Hpy188I TCNGA 8 cut(s) 103, 174, 358, 421, 714, 793, 882, 915
Hpy188III TCNNGA 1 cut(s) 615
Hpy8I GTNNAC 4 cut(s) 48, 81, 148, 331
HpyAV CCTTC 1 cut(s) 796
HpyCH4III ACNGT 5 cut(s) 52, 412, 710, 738, 983
HpyCH4IV ACGT 1 cut(s) 747
HpyCH4V TGCA 8 cut(s) 188, 331, 384, 545, 692, 827, 877, 899
HpyF10VI GCNNNNNNNGC 3 cut(s) 686, 750, 759
HpyF3I CTNAG 4 cut(s) 96, 119, 181, 912
HpySE526I ACGT 1 cut(s) 747
Hsp92II CATG 3 cut(s) 294, 384, 628
HspAI GCGC 1 cut(s) 753
Kzo9I GATC 4 cut(s) 139, 375, 592, 714
LmnI GCTCC 3 cut(s) 835, 850, 860
Lsp1109I GCAGC 2 cut(s) 676, 839
LweI GCATC 4 cut(s) 424, 467, 701, 886
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 747
MaeIII GTNAC 2 cut(s) 620, 835
MalI GATC 4 cut(s) 141, 377, 594, 716
MboI GATC 4 cut(s) 139, 375, 592, 714
MboII GAAGA 5 cut(s) 52, 61, 408, 655, 791
MfeI CAATTG 1 cut(s) 664
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 2 cut(s) 333, 847
MlsI TGGCCA 1 cut(s) 465
MluCI AATT 8 cut(s) 258, 473, 527, 538, 664, 671, 885, 973
MluNI TGGCCA 1 cut(s) 465
MmeI TCCRAC 1 cut(s) 336
Mox20I TGGCCA 1 cut(s) 465
MroXI GAANNNNTTC 2 cut(s) 783, 945
MscI TGGCCA 1 cut(s) 465
MseI TTAA 5 cut(s) 224, 257, 312, 812, 988
MslI CAYNNNNRTG 2 cut(s) 200, 371
Msp20I TGGCCA 1 cut(s) 465
MspI CCGG 3 cut(s) 92, 198, 484
MspR9I CCNGG 7 cut(s) 92, 242, 280, 307, 485, 494, 841
MunI CAATTG 1 cut(s) 664
MvaI CCWGG 5 cut(s) 242, 280, 307, 494, 841
MvnI CGCG 2 cut(s) 65, 85
MwoI GCNNNNNNNGC 3 cut(s) 686, 750, 759
NciI CCSGG 2 cut(s) 92, 485
NdeII GATC 4 cut(s) 139, 375, 592, 714
NlaIII CATG 3 cut(s) 294, 384, 628
NlaIV GGNNCC 1 cut(s) 846
NmuCI GTSAC 1 cut(s) 620
PceI AGGCCT 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdmI GAANNNNTTC 2 cut(s) 783, 945
PfeI GAWTC 2 cut(s) 87, 422
PfoI TCCNGGA 1 cut(s) 483
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 4 cut(s) 11, 691, 775, 829
PshBI ATTAAT 1 cut(s) 257
Psp6I CCWGG 5 cut(s) 240, 278, 305, 492, 839
PspGI CCWGG 5 cut(s) 240, 278, 305, 492, 839
PspN4I GGNNCC 1 cut(s) 846
PspPI GGNCC 1 cut(s) 597
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
RseI CAYNNNNRTG 2 cut(s) 200, 371
SaqAI TTAA 5 cut(s) 224, 257, 312, 812, 988
SatI GCNGC 4 cut(s) 10, 690, 774, 828
Sau3AI GATC 4 cut(s) 139, 375, 592, 714
Sau96I GGNCC 1 cut(s) 597
ScaI AGTACT 1 cut(s) 318
ScrFI CCNGG 7 cut(s) 92, 242, 280, 307, 485, 494, 841
SduI GDGCHC 2 cut(s) 333, 847
SfaNI GCATC 4 cut(s) 424, 467, 701, 886
SfcI CTRYAG 1 cut(s) 402
SmiMI CAYNNNNRTG 2 cut(s) 200, 371
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 8 cut(s) 258, 473, 527, 538, 664, 671, 885, 973
SseBI AGGCCT 1 cut(s) 126
SsiI CCGC 4 cut(s) 9, 65, 773, 776
SspMI CTAG 1 cut(s) 581
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 7 cut(s) 90, 240, 278, 305, 483, 492, 839
TaaI ACNGT 5 cut(s) 52, 412, 710, 738, 983
TaiI ACGT 1 cut(s) 750
TaqI TCGA 2 cut(s) 398, 441
TasI AATT 8 cut(s) 258, 473, 527, 538, 664, 671, 885, 973
TatI WGTACW 1 cut(s) 316
TauI GCSGC 2 cut(s) 12, 776
TfiI GAWTC 2 cut(s) 87, 422
Tru1I TTAA 5 cut(s) 224, 257, 312, 812, 988
Tru9I TTAA 5 cut(s) 224, 257, 312, 812, 988
TscAI CASTG 1 cut(s) 611
TseFI GTSAC 1 cut(s) 620
TseI GCWGC 2 cut(s) 689, 827
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 5 cut(s) 279, 537, 641, 649, 747
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 1 cut(s) 611
VneI GTGCAC 1 cut(s) 329
VspI ATTAAT 1 cut(s) 257
XagI CCTNNNNNAGG 1 cut(s) 722
XapI RAATTY 6 cut(s) 473, 527, 538, 671, 885, 973
XmiI GTMKAC 1 cut(s) 80
XmnI GAANNNNTTC 2 cut(s) 783, 945
XspI CTAG 1 cut(s) 581
ZrmI AGTACT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.