pycom111g00810

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
N/A
Physical Location & Seq
Reverse (-)
552150 .. 552936
787 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGGCGGTTGCGTCCATGGCTTTCAACGTCGTCGGGGCTTTCAGAGGGCTGTCTCTCCGCCCAATCTCCACCTCCACCTCCAGCTCGTCCTCCTCGTCCTTCCTCAGAGGCGACTTGGGTTCTCTGCAAGTGGGTCCCAAGCTGTCGTTAGTGTCGTCGGGGCCCTCAGCGACTGCCATTGACGATTCAAAACGCGCACAAGAAAGGAGGCGGGAGTACCAAGAACGGCCGCGATTCCAAAGGACAGAGGCTCGGCGTCAAGATTTACGGCGACCAGGTCGCCAAGGCTGGCTCCATCATTGTCCGCCAGCGCGGCACCATGTTCCATGCAGGGAAGAATGTGGGGCTTGGCAAGGACCACACCATCTTCTCTTTGATCGATGGCATGGTGAAGTTCGAGAAGTTTGGACCCGACAAGAAGGTATGATCTGGAGTTCCGAAACCCGTCGTAGCTGGTGGTTTGATTGGCTGCGTGTTGCATACTAA

Protein Analysis

162

Amino Acids

18.63

Weight (kDa)

10.85

Isoelectric Point (pI)

79.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 315
AccII CGCG 3 cut(s) 195, 232, 313
AciI CCGC 6 cut(s) 5, 58, 211, 230, 305, 313
AcoI YGGCCR 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 256
AfaI GTAC 1 cut(s) 218
AgsI TTSAA 2 cut(s) 25, 189
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 3 cut(s) 84, 142, 453
AluI AGCT 3 cut(s) 84, 142, 453
Alw26I GTCTC 1 cut(s) 57
AlwNI CAGNNNCTG 1 cut(s) 173
AoxI GGCC 2 cut(s) 161, 227
ApaI GGGCCC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 469
AspLEI GCGC 2 cut(s) 197, 313
AspS9I GGNCC 5 cut(s) 134, 161, 162, 356, 408
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 3 cut(s) 134, 356, 408
BaeGI GKGCMC 1 cut(s) 165
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 165
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 456
BccI CCATC 3 cut(s) 303, 372, 375
BceAI ACGGC 2 cut(s) 242, 284
BciT130I CCWGG 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 57
BglI GCCNNNNNGGC 1 cut(s) 313
BisI GCNGC 3 cut(s) 230, 314, 470
BlsI GCNGC 3 cut(s) 231, 315, 471
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 3 cut(s) 134, 356, 408
BmgT120I GGNCC 5 cut(s) 134, 161, 162, 356, 408
BmiI GGNNCC 7 cut(s) 135, 136, 162, 163, 293, 317, 410
BmrFI CCNGG 1 cut(s) 276
BpmI CTGGAG 2 cut(s) 64, 451
Bpu10I CCTNAGC 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 379
BsaHI GRCGYC 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 15, 283
BseBI CCWGG 1 cut(s) 276
BseCI ATCGAT 1 cut(s) 379
BseDI CCNNGG 2 cut(s) 15, 283
BseMII CTCAG 2 cut(s) 118, 180
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 227
BseXI GCAGC 1 cut(s) 456
Bsh1236I CGCG 3 cut(s) 195, 232, 313
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 2 cut(s) 163, 229
BshNI GGYRCC 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 379
BsiEI CGRYCG 1 cut(s) 230
BslFI GGGAC 1 cut(s) 120
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 120
BsnI GGCC 2 cut(s) 163, 229
Bsp120I GGGCCC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 2 cut(s) 376, 426
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 6 cut(s) 5, 58, 211, 230, 305, 313
BspANI GGCC 2 cut(s) 163, 229
BspCNI CTCAG 2 cut(s) 117, 179
BspDI ATCGAT 1 cut(s) 379
BspFNI CGCG 3 cut(s) 195, 232, 313
BspLI GGNNCC 7 cut(s) 135, 136, 162, 163, 293, 317, 410
BspT107I GGYRCC 1 cut(s) 315
BssECI CCNNGG 2 cut(s) 15, 283
BssMI GATC 2 cut(s) 376, 426
BssNI GRCGYC 1 cut(s) 256
BssT1I CCWWGG 2 cut(s) 15, 283
Bst2UI CCWGG 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 290, 309
BstDEI CTNAG 2 cut(s) 104, 166
BstDSI CCRYGG 1 cut(s) 15
BstFNI CGCG 3 cut(s) 195, 232, 313
BstHHI GCGC 2 cut(s) 197, 313
BstKTI GATC 2 cut(s) 379, 429
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 2 cut(s) 376, 426
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 2 cut(s) 17, 313
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 1 cut(s) 274
BstSLI GKGCMC 1 cut(s) 165
BstUI CGCG 3 cut(s) 195, 232, 313
BstV1I GCAGC 1 cut(s) 456
BstZI CGGCCG 1 cut(s) 227
Bsu15I ATCGAT 1 cut(s) 379
BsuRI GGCC 2 cut(s) 163, 229
BsuTUI ATCGAT 1 cut(s) 379
BtgI CCRYGG 1 cut(s) 15
Cac8I GCNNGC 2 cut(s) 290, 309
CaiI CAGNNNCTG 1 cut(s) 173
CfoI GCGC 2 cut(s) 197, 313
Cfr13I GGNCC 5 cut(s) 134, 161, 162, 356, 408
ClaI ATCGAT 1 cut(s) 379
CseI GACGC 1 cut(s) 245
CsiI ACCWGGT 1 cut(s) 274
Csp6I GTAC 1 cut(s) 217
CviAII CATG 4 cut(s) 16, 320, 327, 386
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 2 cut(s) 104, 166
DpnI GATC 2 cut(s) 378, 428
DpnII GATC 2 cut(s) 376, 426
EaeI YGGCCR 1 cut(s) 227
EagI CGGCCG 1 cut(s) 227
EciI GGCGGA 2 cut(s) 47, 294
EclXI CGGCCG 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 15, 283
Eco24I GRGCYC 1 cut(s) 165
Eco47I GGWCC 3 cut(s) 134, 356, 408
Eco52I CGGCCG 1 cut(s) 227
EcoO109I RGGNCCY 3 cut(s) 134, 161, 162
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 2 cut(s) 15, 283
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 2 cut(s) 15, 283
FaeI CATG 4 cut(s) 19, 323, 330, 389
FaiI YATR 6 cut(s) 17, 321, 328, 387, 425, 481
FaqI GGGAC 1 cut(s) 120
FatI CATG 4 cut(s) 15, 319, 326, 385
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 230, 314, 470
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 3 cut(s) 230, 314, 470
GlaI GCGC 2 cut(s) 196, 312
GluI GCNGC 3 cut(s) 230, 314, 470
GsuI CTGGAG 2 cut(s) 64, 451
HaeIII GGCC 2 cut(s) 163, 229
HgaI GACGC 1 cut(s) 245
HhaI GCGC 2 cut(s) 197, 313
Hin1I GRCGYC 1 cut(s) 256
Hin1II CATG 4 cut(s) 19, 323, 330, 389
Hin6I GCGC 2 cut(s) 195, 311
HinP1I GCGC 2 cut(s) 195, 311
HinfI GANTC 2 cut(s) 185, 234
HphI GGTGA 1 cut(s) 401
Hpy188I TCNGA 3 cut(s) 44, 107, 439
Hpy188III TCNNGA 3 cut(s) 260, 398, 430
Hpy99I CGWCG 4 cut(s) 32, 35, 160, 450
HpyAV CCTTC 2 cut(s) 109, 413
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 3 cut(s) 127, 330, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 313
HpyF3I CTNAG 2 cut(s) 104, 166
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 256
Hsp92II CATG 4 cut(s) 19, 323, 330, 389
HspAI GCGC 2 cut(s) 195, 311
KflI GGGWCCC 1 cut(s) 134
Kzo9I GATC 2 cut(s) 376, 426
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 8 cut(s) 94, 261, 274, 288, 316, 321, 415, 439
Lsp1109I GCAGC 1 cut(s) 456
MabI ACCWGGT 1 cut(s) 274
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 378, 428
MboI GATC 2 cut(s) 376, 426
MboII GAAGA 2 cut(s) 347, 359
MhlI GDGCHC 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 276
MvaI CCWGG 1 cut(s) 276
MvnI CGCG 3 cut(s) 195, 232, 313
MwoI GCNNNNNNNGC 2 cut(s) 17, 313
NcoI CCATGG 1 cut(s) 15
NdeII GATC 2 cut(s) 376, 426
NlaIII CATG 4 cut(s) 19, 323, 330, 389
NlaIV GGNNCC 7 cut(s) 135, 136, 162, 163, 293, 317, 410
NmeAIII GCCGAG 1 cut(s) 232
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 152
PfeI GAWTC 2 cut(s) 185, 234
PflFI GACNNNGTC 1 cut(s) 276
PkrI GCNGC 3 cut(s) 231, 315, 471
PpuMI RGGWCCY 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 7 cut(s) 135, 136, 162, 163, 293, 317, 410
PspOMI GGGCCC 1 cut(s) 161
PspPI GGNCC 5 cut(s) 134, 161, 162, 356, 408
PspPPI RGGWCCY 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 276
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SatI GCNGC 3 cut(s) 230, 314, 470
Sau3AI GATC 2 cut(s) 376, 426
Sau96I GGNCC 5 cut(s) 134, 161, 162, 356, 408
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 1 cut(s) 165
SetI ASST 8 cut(s) 30, 74, 80, 86, 144, 280, 424, 455
SexAI ACCWGGT 1 cut(s) 274
SinI GGWCC 3 cut(s) 134, 356, 408
SsiI CCGC 6 cut(s) 5, 58, 211, 230, 305, 313
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 2 cut(s) 15, 283
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 379, 397
TauI GCSGC 2 cut(s) 232, 316
TfiI GAWTC 2 cut(s) 185, 234
TseI GCWGC 1 cut(s) 469
Tth111I GACNNNGTC 1 cut(s) 276
VpaK11BI GGWCC 3 cut(s) 134, 356, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.