pycom111g03130

Chloroplastic group IIA intron splicing facilitator CRS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0028459
Physical Location & Seq
Reverse (-)
1898471 .. 1899071
601 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 387 bp
ATGAGAGGCTTCTTCTGCAAAACAAGCCCTCAAGATGAAAAATCGCCCTGTGATTACAGTCGCTTCTGTTATCACCTTTTAGGAAGGAAAAATATTGTAGCTGGTGTCGCTAAGACAATCAAGCAGCACTTTGAGAAGCACCCTCTTGCCATAGTGAACGTCAAAGGCATAGCAAAAGAAACTTCGGCCTTGGAAGTGGTTTTCAAACTCGAGCAAGCAACAGGTGCAGTGTTAGTCTCCCACGAGCCTAGCAAAGTTGTACTTTACAGGGGTTGGGGCGCAGGAGATGATCCCGGCCTTAATGATAGGAACGGAATTAACACAGGGAAAAAAGGCTTCTTCTCAGGGTGTTGTGTCTCCCGAACTCCTGGGAGCAATAAGACTTGA

Protein Analysis

129

Amino Acids

13.94

Weight (kDa)

9.42

Isoelectric Point (pI)

19.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 26 - 90 1.1e-10 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028459)

Species Orthologous Gene IDs
malus_domestica MD09G1040000.v1.1
pyrus_communis pycom111g03130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 284
AfaI GTAC 1 cut(s) 261
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 1 cut(s) 101
AluI AGCT 1 cut(s) 101
Alw26I GTCTC 2 cut(s) 241, 361
AlwI GGATC 1 cut(s) 284
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 2 cut(s) 186, 295
ApeKI GCWGC 1 cut(s) 124
AspLEI GCGC 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 209
BauI CACGAG 1 cut(s) 242
BbvI GCAGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 369
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 241, 361
BfaI CTAG 1 cut(s) 249
BisI GCNGC 1 cut(s) 125
BlsI GCNGC 1 cut(s) 126
Bme1390I CCNGG 2 cut(s) 294, 369
BmeT110I CYCGRG 1 cut(s) 209
BmrFI CCNGG 2 cut(s) 294, 369
BpuEI CTTGAG 1 cut(s) 15
BpuMI CCSGG 1 cut(s) 294
BsaJI CCNNGG 2 cut(s) 189, 368
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 189, 368
BseMII CTCAG 1 cut(s) 357
BseXI GCAGC 1 cut(s) 136
BsgI GTGCAG 1 cut(s) 246
BshFI GGCC 2 cut(s) 188, 297
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 1 cut(s) 294
BsmAI GTCTC 2 cut(s) 241, 361
BsnI GGCC 2 cut(s) 188, 297
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 1 cut(s) 289
BspANI GGCC 2 cut(s) 188, 297
BspCNI CTCAG 1 cut(s) 356
BspPI GGATC 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 189, 368
BssMI GATC 1 cut(s) 289
BssSI CACGAG 1 cut(s) 242
BssT1I CCWWGG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 111, 343
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 1 cut(s) 292
BstMAI GTCTC 2 cut(s) 241, 361
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 4 cut(s) 15, 24, 107, 224
BstNI CCWGG 1 cut(s) 369
BstSCI CCNGG 2 cut(s) 292, 367
BstV1I GCAGC 1 cut(s) 136
BsuRI GGCC 2 cut(s) 188, 297
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 260
CviJI RGCY 7 cut(s) 9, 27, 101, 188, 247, 297, 336
CviKI_1 RGCY 7 cut(s) 9, 27, 101, 188, 247, 297, 336
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 2 cut(s) 111, 343
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
FaiI YATR 2 cut(s) 152, 170
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 246, 278
Fnu4HI GCNGC 1 cut(s) 125
Fsp4HI GCNGC 1 cut(s) 125
FspBI CTAG 1 cut(s) 249
GlaI GCGC 1 cut(s) 280
GluI GCNGC 1 cut(s) 125
HaeIII GGCC 2 cut(s) 188, 297
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 281
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HpaII CCGG 1 cut(s) 294
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 1 cut(s) 157
Hpy188III TCNNGA 2 cut(s) 32, 360
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 78
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 2 cut(s) 18, 227
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 24, 107, 224
HpyF3I CTNAG 2 cut(s) 111, 343
HpySE526I ACGT 1 cut(s) 159
HspAI GCGC 1 cut(s) 279
Kzo9I GATC 1 cut(s) 289
LmnI GCTCC 1 cut(s) 372
Lsp1109I GCAGC 1 cut(s) 136
MaeI CTAG 1 cut(s) 249
MaeII ACGT 1 cut(s) 159
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 2 cut(s) 4, 331
MluCI AATT 1 cut(s) 315
MnlI CCTC 2 cut(s) 39, 153
MseI TTAA 2 cut(s) 300, 318
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 294, 369
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 4 cut(s) 15, 24, 107, 224
NciI CCSGG 1 cut(s) 294
NdeII GATC 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 209
PkrI GCNGC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspXI VCTCGAGB 1 cut(s) 209
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
SaqAI TTAA 2 cut(s) 300, 318
SatI GCNGC 1 cut(s) 125
Sau3AI GATC 1 cut(s) 289
ScrFI CCNGG 2 cut(s) 294, 369
SetI ASST 4 cut(s) 78, 103, 162, 226
Sfr274I CTCGAG 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 209
SmlI CTYRAG 2 cut(s) 30, 209
SmoI CTYRAG 2 cut(s) 30, 209
Sse9I AATT 1 cut(s) 315
SspI AATATT 1 cut(s) 94
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 292, 367
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 315
TatI WGTACW 1 cut(s) 259
Tru1I TTAA 2 cut(s) 300, 318
Tru9I TTAA 2 cut(s) 300, 318
TscAI CASTG 1 cut(s) 234
TseI GCWGC 1 cut(s) 124
TspDTI ATGAA 1 cut(s) 51
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 1 cut(s) 234
XhoI CTCGAG 1 cut(s) 209
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.