pycom111g05150

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
N/A
Physical Location & Seq
Reverse (-)
3309641 .. 3310313
673 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 633 bp
ATGGGTGTGCTGACATGCAACAGGTGGACCCGAGTTCTCCGGCGCCAGAACTCCCAGATCGACGACGCCGACAACAACAACAACTCCAACAACATTGAAGACATCATCAACGCCCCAATTATCAGCAACTCTGTCGAATGCTACGCCTGCACTCAGGTGGGGGTCCCGGCCTTCCATTCCACCAGCTGCGACGGCGCCCACCAGCCCCAGTGGGAGGCCTCCGCCGGCTCTTCCCTGATTCCCATCCAATCCCGCTCCAACCTTAAAAAAGGAATCTCCCGCCACCGCCCAGCCGGTCCCTTCGGCCCCGTCCTCGACCCCCGCAGTAAGCGCGTCCAGCGCTGGAACCGCGCGTTCCTCCTCGCACGTGGGATGGCGCTGGCCGTCGACCCGCTCTTCTTCTACGCCCTCTCTATCGGAAGAAGCGGAACCCCCTGCCTCTACATGGACGGAGGGCTGGCAGCAATTGTCACCGTGCTCCGCACGAGCGTGGACGCCGTGCACCTGTGCCACCTGTGGCTGCAGTTCCGGCTGGCGTACGTGTCGAGGGAGTCGCTGGTGGTCGGCTGCGGAAAGCTCGTGTGGGACCCACGTGCGATCTCCTACCACTACCTTCGATCTCTCAAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

211

Amino Acids

23.24

Weight (kDa)

9.5

Isoelectric Point (pI)

66.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 42, 194
AccBSI CCGCTC 2 cut(s) 255, 394
AccI GTMKAC 1 cut(s) 387
AccII CGCG 3 cut(s) 333, 351, 353
AcoI YGGCCR 1 cut(s) 381
AcvI CACGTG 2 cut(s) 368, 593
AcyI GRCGYC 4 cut(s) 43, 66, 195, 495
AfaI GTAC 1 cut(s) 539
AfeI AGCGCT 1 cut(s) 341
AfiI CCNNNNNNNGG 2 cut(s) 214, 445
AflIII ACRYGT 1 cut(s) 540
AgsI TTSAA 1 cut(s) 98
AleI CACNNNNGTG 2 cut(s) 155, 488
AluBI AGCT 2 cut(s) 186, 577
AluI AGCT 2 cut(s) 186, 577
Alw21I GWGCWC 2 cut(s) 480, 504
Alw44I GTGCAC 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 30
Aor51HI AGCGCT 1 cut(s) 341
AoxI GGCC 4 cut(s) 168, 216, 304, 381
ApaLI GTGCAC 1 cut(s) 500
ApeKI GCWGC 4 cut(s) 186, 461, 520, 567
AspLEI GCGC 6 cut(s) 45, 197, 333, 342, 353, 379
AspS9I GGNCC 5 cut(s) 27, 163, 296, 305, 586
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 463
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 4 cut(s) 27, 163, 296, 586
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 2 cut(s) 42, 194
BauI CACGAG 2 cut(s) 484, 578
BbrPI CACGTG 2 cut(s) 368, 593
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 2 cut(s) 480, 504
BbvI GCAGC 4 cut(s) 173, 473, 507, 554
BccI CCATC 2 cut(s) 251, 367
BceAI ACGGC 3 cut(s) 208, 368, 482
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BcnI CCSGG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 521
BfoI RGCGCY 4 cut(s) 46, 198, 343, 380
BisI GCNGC 4 cut(s) 187, 462, 521, 568
BlsI GCNGC 4 cut(s) 188, 463, 522, 569
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 4 cut(s) 27, 163, 296, 586
BmeT110I CYCGRG 1 cut(s) 30
BmgT120I GGNCC 5 cut(s) 27, 163, 296, 305, 586
BmrFI CCNGG 1 cut(s) 167
BmrI ACTGGG 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 167
BsaAI YACGTR 3 cut(s) 368, 541, 593
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 4 cut(s) 43, 66, 195, 495
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 445
Bse118I RCCGGY 2 cut(s) 224, 293
Bse1I ACTGG 1 cut(s) 208
Bse8I GATNNNNATC 1 cut(s) 242
BseGI GGATG 2 cut(s) 243, 378
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 214, 445
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 4 cut(s) 173, 473, 507, 554
BseYI CCCAGC 1 cut(s) 289
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 3 cut(s) 333, 351, 353
BshFI GGCC 4 cut(s) 170, 218, 306, 383
BshNI GGYRCC 2 cut(s) 42, 194
BsiHKAI GWGCWC 2 cut(s) 480, 504
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 5 cut(s) 40, 167, 225, 294, 529
BsiWI CGTACG 1 cut(s) 537
BslFI GGGAC 3 cut(s) 149, 282, 599
BslI CCNNNNNNNGG 2 cut(s) 214, 445
BsmFI GGGAC 3 cut(s) 149, 282, 599
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 4 cut(s) 170, 218, 306, 383
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 480, 504
Bsp143I GATC 3 cut(s) 57, 597, 617
BspANI GGCC 4 cut(s) 170, 218, 306, 383
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 3 cut(s) 333, 351, 353
BspMAI CTGCAG 1 cut(s) 525
BspQI GCTCTTC 2 cut(s) 235, 401
BspT107I GGYRCC 2 cut(s) 42, 194
BsrBI CCGCTC 2 cut(s) 255, 394
BsrFI RCCGGY 2 cut(s) 224, 293
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 2 cut(s) 224, 293
BssMI GATC 3 cut(s) 57, 597, 617
BssNI GRCGYC 4 cut(s) 43, 66, 195, 495
BssSI CACGAG 2 cut(s) 484, 578
Bst2BI CACGAG 2 cut(s) 484, 578
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 2 cut(s) 235, 401
BstACI GRCGYC 4 cut(s) 43, 66, 195, 495
BstBAI YACGTR 3 cut(s) 368, 541, 593
BstC8I GCNNGC 5 cut(s) 148, 226, 381, 459, 534
BstDEI CTNAG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 243, 378
BstFNI CGCG 3 cut(s) 333, 351, 353
BstH2I RGCGCY 4 cut(s) 46, 198, 343, 380
BstHHI GCGC 6 cut(s) 45, 197, 333, 342, 353, 379
BstKTI GATC 3 cut(s) 60, 600, 620
BstMBI GATC 3 cut(s) 57, 597, 617
BstMWI GCNNNNNNNGC 7 cut(s) 147, 192, 330, 337, 339, 348, 529
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 521
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 3 cut(s) 333, 351, 353
BstV1I GCAGC 4 cut(s) 173, 473, 507, 554
BstV2I GAAGAC 1 cut(s) 105
BsuRI GGCC 4 cut(s) 170, 218, 306, 383
BtsCI GGATG 2 cut(s) 243, 378
BtsIMutI CAGTG 1 cut(s) 215
Cac8I GCNNGC 5 cut(s) 148, 226, 381, 459, 534
CfoI GCGC 6 cut(s) 45, 197, 333, 342, 353, 379
Cfr10I RCCGGY 2 cut(s) 224, 293
Cfr13I GGNCC 5 cut(s) 27, 163, 296, 305, 586
CseI GACGC 3 cut(s) 74, 322, 503
Csp6I GTAC 1 cut(s) 538
CviAII CATG 2 cut(s) 15, 445
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 1 cut(s) 153
DinI GGCGCC 2 cut(s) 44, 196
DpnI GATC 3 cut(s) 59, 599, 619
DpnII GATC 3 cut(s) 57, 597, 617
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 2 cut(s) 235, 401
EarI CTCTTC 2 cut(s) 235, 401
EciI GGCGGA 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 218
Eco47I GGWCC 4 cut(s) 27, 163, 296, 586
Eco47III AGCGCT 1 cut(s) 341
Eco72I CACGTG 2 cut(s) 368, 593
Eco88I CYCGRG 1 cut(s) 30
EcoO109I RGGNCCY 2 cut(s) 163, 586
EgeI GGCGCC 2 cut(s) 44, 196
EheI GGCGCC 2 cut(s) 44, 196
FaeI CATG 2 cut(s) 18, 448
FaiI YATR 2 cut(s) 16, 446
FaqI GGGAC 3 cut(s) 149, 282, 599
FatI CATG 2 cut(s) 14, 444
FauI CCCGC 4 cut(s) 260, 287, 329, 399
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 4 cut(s) 187, 462, 521, 568
FokI GGATG 2 cut(s) 230, 385
Fsp4HI GCNGC 4 cut(s) 187, 462, 521, 568
GlaI GCGC 6 cut(s) 44, 196, 332, 341, 352, 378
GluI GCNGC 4 cut(s) 187, 462, 521, 568
GsaI CCCAGC 1 cut(s) 293
HaeII RGCGCY 4 cut(s) 46, 198, 343, 380
HaeIII GGCC 4 cut(s) 170, 218, 306, 383
HapII CCGG 5 cut(s) 40, 167, 225, 294, 529
HgaI GACGC 3 cut(s) 74, 322, 503
HhaI GCGC 6 cut(s) 45, 197, 333, 342, 353, 379
Hin1I GRCGYC 4 cut(s) 43, 66, 195, 495
Hin1II CATG 2 cut(s) 18, 448
Hin6I GCGC 6 cut(s) 43, 195, 331, 340, 351, 377
HinP1I GCGC 6 cut(s) 43, 195, 331, 340, 351, 377
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 3 cut(s) 238, 273, 551
HpaII CCGG 5 cut(s) 40, 167, 225, 294, 529
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 4 cut(s) 27, 388, 493, 502
Hpy188I TCNGA 1 cut(s) 419
Hpy8I GTNNAC 4 cut(s) 27, 388, 493, 502
Hpy99I CGWCG 4 cut(s) 65, 68, 194, 389
HpyAV CCTTC 3 cut(s) 181, 310, 623
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 3 cut(s) 367, 540, 592
HpyCH4V TGCA 4 cut(s) 18, 150, 502, 523
HpyF10VI GCNNNNNNNGC 7 cut(s) 147, 192, 330, 337, 339, 348, 529
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 3 cut(s) 367, 540, 592
Hsp92I GRCGYC 4 cut(s) 43, 66, 195, 495
Hsp92II CATG 2 cut(s) 18, 448
HspAI GCGC 6 cut(s) 43, 195, 331, 340, 351, 377
KasI GGCGCC 2 cut(s) 42, 194
KflI GGGWCCC 2 cut(s) 163, 586
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 57, 597, 617
LguI GCTCTTC 2 cut(s) 235, 401
LmnI GCTCC 2 cut(s) 260, 483
Lsp1109I GCAGC 4 cut(s) 173, 473, 507, 554
MaeII ACGT 3 cut(s) 367, 540, 592
MaeIII GTNAC 1 cut(s) 469
MalI GATC 3 cut(s) 59, 599, 619
MbiI CCGCTC 2 cut(s) 255, 394
MboI GATC 3 cut(s) 57, 597, 617
MboII GAAGA 5 cut(s) 110, 222, 388, 391, 432
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 2 cut(s) 480, 504
MluCI AATT 2 cut(s) 117, 465
Mly113I GGCGCC 2 cut(s) 43, 195
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 2 cut(s) 111, 282
MnlI CCTC 9 cut(s) 208, 229, 323, 368, 371, 419, 446, 449, 540
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 2 cut(s) 155, 488
MspA1I CMGCKG 1 cut(s) 186
MspI CCGG 5 cut(s) 40, 167, 225, 294, 529
MspR9I CCNGG 1 cut(s) 167
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 1 cut(s) 143
MvnI CGCG 3 cut(s) 333, 351, 353
MwoI GCNNNNNNNGC 7 cut(s) 147, 192, 330, 337, 339, 348, 529
NaeI GCCGGC 1 cut(s) 226
NarI GGCGCC 2 cut(s) 43, 195
NciI CCSGG 1 cut(s) 167
NdeII GATC 3 cut(s) 57, 597, 617
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 2 cut(s) 18, 448
NmuCI GTSAC 1 cut(s) 469
NspI RCATGY 1 cut(s) 18
OliI CACNNNNGTG 2 cut(s) 155, 488
PceI AGGCCT 1 cut(s) 218
PciSI GCTCTTC 2 cut(s) 235, 401
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 143
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 238, 273
Pfl23II CGTACG 1 cut(s) 537
PkrI GCNGC 4 cut(s) 188, 463, 522, 569
PleI GAGTC 1 cut(s) 559
PluTI GGCGCC 2 cut(s) 46, 198
PmaCI CACGTG 2 cut(s) 368, 593
PmlI CACGTG 2 cut(s) 368, 593
PpsI GAGTC 1 cut(s) 559
Ppu21I YACGTR 3 cut(s) 368, 541, 593
PpuMI RGGWCCY 2 cut(s) 163, 586
Psp5II RGGWCCY 2 cut(s) 163, 586
PspCI CACGTG 2 cut(s) 368, 593
PspFI CCCAGC 1 cut(s) 289
PspLI CGTACG 1 cut(s) 537
PspPI GGNCC 5 cut(s) 27, 163, 296, 305, 586
PspPPI RGGWCCY 2 cut(s) 163, 586
PstI CTGCAG 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 186
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 2 cut(s) 155, 488
SalI GTCGAC 1 cut(s) 386
SapI GCTCTTC 2 cut(s) 235, 401
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 4 cut(s) 187, 462, 521, 568
Sau3AI GATC 3 cut(s) 57, 597, 617
Sau96I GGNCC 5 cut(s) 27, 163, 296, 305, 586
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 2 cut(s) 480, 504
SfcI CTRYAG 1 cut(s) 521
SfoI GGCGCC 2 cut(s) 44, 196
SinI GGWCC 4 cut(s) 27, 163, 296, 586
SmiMI CAYNNNNRTG 2 cut(s) 155, 488
Sse9I AATT 2 cut(s) 117, 465
SseBI AGGCCT 1 cut(s) 218
SspDI GGCGCC 2 cut(s) 42, 194
StuI AGGCCT 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 3 cut(s) 370, 543, 595
TaqI TCGA 6 cut(s) 60, 135, 315, 387, 545, 616
TasI AATT 2 cut(s) 117, 465
TfiI GAWTC 2 cut(s) 238, 273
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 215
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 4 cut(s) 186, 461, 520, 567
Tsp45I GTSAC 1 cut(s) 469
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 1 cut(s) 215
VneI GTGCAC 1 cut(s) 500
VpaK11BI GGWCC 4 cut(s) 27, 163, 296, 586
XceI RCATGY 1 cut(s) 18
XmiI GTMKAC 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.