pycom11g00370

Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Reverse (-)
293281 .. 294503
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 762 bp
ATGAAGGGTGGCTTAGGACAACCTTCTGTCAGAAAGCAGCTCGTGGGCACACAGTTAATGGACAGCTCAAGGAGTTTTATTCAGTGGGTGCAAAGTATCAGCTTTATTACAATTGCAATTGATCATTCTTTGCATCTTATTTTTCAAGCAGATACACAATCTCCGGGAGCTCAAGGTGTTGTCGGTGTGGGCTCCGCAGCATATCTGGAAGGCTTCACACCCGTGAAGTCTCTTGGTTCCAAACCTGCTTCTTACATTCTGATCCCATCACCGAGCATGCGATTCCTCCCGCCAACACCTCTTCAGCTGCCCACATGCCTCACTGCCGAATCACCTCCAGTAGCCCATCTCCTTCACAGTAAAGGCTCTGCTATCCCCTTTTCTACTTGTTTTGTGGTTTCCAAAGCTGCACCTACTGTGAGGAGAGATTGGAGGAGCAAAGAAGAATGGCCTTCATCTCTTTTGGTCAGTCTTATTGATCATTATGGAGGCAATAGCTTTAGCCAAGACAAACTCATGAGGGGTAATACCAAACAAGCGGGAAGGAGTCCAAGCTCGGAAGCTAGAGAGTTGGAGTTTGAGATTCATGTCATTCTGGAATCTCTGGCGGCAGAGCTTCATGCACTCTCATGGATGACTGTGAGTCCAGTGTACCTAGAGAGGCGAACTGCACTGCCATTTCATTGCGATATGGTTTTGAGATTGAGGAGGCTTTTGCATTTTGCTGATAAGGAGCTGTCCAGGCATCAAGAGAAGGCTTAG

Protein Analysis

254

Amino Acids

27.91

Weight (kDa)

9.22

Isoelectric Point (pI)

61.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 253
AciI CCGC 4 cut(s) 195, 290, 539, 608
AclWI GGATC 1 cut(s) 256
AcuI CTGAAG 1 cut(s) 287
AfaI GTAC 1 cut(s) 653
AgsI TTSAA 1 cut(s) 146
AhdI GACNNNNNGTC 1 cut(s) 642
AjnI CCWGG 1 cut(s) 740
AleI CACNNNNGTG 1 cut(s) 221
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 1 cut(s) 256
AoxI GGCC 1 cut(s) 449
ApeKI GCWGC 4 cut(s) 37, 197, 307, 407
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 261, 324
BaeGI GKGCMC 1 cut(s) 50
BanII GRGCYC 2 cut(s) 172, 194
BauI CACGAG 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 4 cut(s) 49, 209, 294, 394
BccI CCATC 2 cut(s) 274, 354
BciT130I CCWGG 1 cut(s) 742
BclI TGATCA 2 cut(s) 121, 478
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 2 cut(s) 564, 656
BfuAI ACCTGC 1 cut(s) 253
BisI GCNGC 5 cut(s) 38, 198, 308, 408, 609
BlsI GCNGC 5 cut(s) 39, 199, 309, 409, 610
Bme1390I CCNGG 2 cut(s) 165, 742
BmeRI GACNNNNNGTC 1 cut(s) 642
BmiI GGNNCC 2 cut(s) 193, 238
BmrFI CCNGG 2 cut(s) 165, 742
BmsI GCATC 2 cut(s) 142, 754
BpmI CTGGAG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 13
BpuEI CTTGAG 2 cut(s) 52, 156
BpuMI CCSGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bse1I ACTGG 2 cut(s) 338, 647
Bse3DI GCAATG 1 cut(s) 682
BseBI CCWGG 1 cut(s) 742
BseGI GGATG 1 cut(s) 639
BseMI GCAATG 1 cut(s) 682
BseNI ACTGG 2 cut(s) 338, 647
BseRI GAGGAG 3 cut(s) 436, 448, 721
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 4 cut(s) 49, 209, 294, 394
BsgI GTGCAG 2 cut(s) 393, 654
BshFI GGCC 1 cut(s) 451
BsiHKAI GWGCWC 1 cut(s) 172
BsiSI CCGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 234
BsnI GGCC 1 cut(s) 451
Bsp1286I GDGCHC 3 cut(s) 50, 172, 194
Bsp143I GATC 3 cut(s) 121, 261, 478
BspACI CCGC 4 cut(s) 195, 290, 539, 608
BspANI GGCC 1 cut(s) 451
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 2 cut(s) 193, 238
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 2 cut(s) 338, 647
BssMI GATC 3 cut(s) 121, 261, 478
BssSI CACGAG 1 cut(s) 41
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 742
Bst4CI ACNGT 4 cut(s) 54, 359, 418, 640
Bst6I CTCTTC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 278
BstDEI CTNAG 2 cut(s) 13, 759
BstF5I GGATG 1 cut(s) 639
BstKTI GATC 3 cut(s) 124, 264, 481
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 3 cut(s) 121, 261, 478
BstMWI GCNNNNNNNGC 1 cut(s) 742
BstNI CCWGG 1 cut(s) 742
BstNSI RCATGY 2 cut(s) 280, 318
BstSCI CCNGG 2 cut(s) 163, 740
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 4 cut(s) 49, 209, 294, 394
BsuRI GGCC 1 cut(s) 451
BtsCI GGATG 1 cut(s) 639
BtsI GCAGTG 2 cut(s) 321, 671
BtsIMutI CAGTG 4 cut(s) 89, 321, 654, 671
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 278
CciI TCATGA 1 cut(s) 516
Csp6I GTAC 1 cut(s) 652
CviAII CATG 6 cut(s) 277, 315, 517, 587, 620, 630
CviQI GTAC 1 cut(s) 652
DdeI CTNAG 2 cut(s) 13, 759
DpnI GATC 3 cut(s) 123, 263, 480
DpnII GATC 3 cut(s) 121, 261, 478
DriI GACNNNNNGTC 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 306
Eam1105I GACNNNNNGTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 306
Ecl136II GAGCTC 1 cut(s) 170
Eco24I GRGCYC 2 cut(s) 172, 194
Eco53kI GAGCTC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 740
EcoT38I GRGCYC 2 cut(s) 172, 194
FaeI CATG 6 cut(s) 280, 318, 520, 590, 623, 633
FaiI YATR 9 cut(s) 202, 278, 316, 486, 518, 588, 621, 631, 692
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 6 cut(s) 276, 314, 516, 586, 619, 629
FauI CCCGC 2 cut(s) 297, 532
FbaI TGATCA 2 cut(s) 121, 478
Fnu4HI GCNGC 5 cut(s) 38, 198, 308, 408, 609
FokI GGATG 1 cut(s) 646
FriOI GRGCYC 2 cut(s) 172, 194
Fsp4HI GCNGC 5 cut(s) 38, 198, 308, 408, 609
FspBI CTAG 2 cut(s) 564, 656
GluI GCNGC 5 cut(s) 38, 198, 308, 408, 609
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 1 cut(s) 451
HapII CCGG 1 cut(s) 164
Hin1II CATG 6 cut(s) 280, 318, 520, 590, 623, 633
HinfI GANTC 6 cut(s) 282, 329, 547, 583, 599, 643
HpaII CCGG 1 cut(s) 164
HphI GGTGA 2 cut(s) 261, 324
Hpy166II GTNNAC 1 cut(s) 652
Hpy188I TCNGA 3 cut(s) 32, 261, 559
Hpy188III TCNNGA 4 cut(s) 206, 517, 596, 749
Hpy8I GTNNAC 1 cut(s) 652
HpyAV CCTTC 6 cut(s) 33, 203, 362, 462, 537, 748
HpyCH4III ACNGT 4 cut(s) 54, 359, 418, 640
HpyCH4V TGCA 7 cut(s) 91, 116, 133, 410, 623, 671, 718
HpyF10VI GCNNNNNNNGC 1 cut(s) 742
HpyF3I CTNAG 2 cut(s) 13, 759
Hsp92II CATG 6 cut(s) 280, 318, 520, 590, 623, 633
Ksp22I TGATCA 2 cut(s) 121, 478
Kzo9I GATC 3 cut(s) 121, 261, 478
LmnI GCTCC 4 cut(s) 167, 197, 435, 733
LpnPI CCDG 9 cut(s) 177, 191, 258, 351, 581, 590, 660, 727, 754
Lsp1109I GCAGC 4 cut(s) 49, 209, 294, 394
LweI GCATC 2 cut(s) 142, 754
MaeI CTAG 2 cut(s) 564, 656
MalI GATC 3 cut(s) 123, 263, 480
MboI GATC 3 cut(s) 121, 261, 478
MboII GAAGA 2 cut(s) 293, 455
MfeI CAATTG 2 cut(s) 111, 117
MhlI GDGCHC 3 cut(s) 50, 172, 194
MluCI AATT 2 cut(s) 111, 117
MlyI GAGTC 2 cut(s) 556, 652
MmeI TCCRAC 1 cut(s) 552
MseI TTAA 1 cut(s) 56
MslI CAYNNNNRTG 2 cut(s) 221, 628
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 165, 742
MunI CAATTG 2 cut(s) 111, 117
MvaI CCWGG 1 cut(s) 742
MwoI GCNNNNNNNGC 1 cut(s) 742
NciI CCSGG 1 cut(s) 165
NdeII GATC 3 cut(s) 121, 261, 478
NlaIII CATG 6 cut(s) 280, 318, 520, 590, 623, 633
NlaIV GGNNCC 2 cut(s) 193, 238
NspI RCATGY 2 cut(s) 280, 318
OliI CACNNNNGTG 1 cut(s) 221
PaeI GCATGC 1 cut(s) 280
PagI TCATGA 1 cut(s) 516
PfeI GAWTC 4 cut(s) 282, 329, 583, 599
PfoI TCCNGGA 1 cut(s) 163
PkrI GCNGC 5 cut(s) 39, 199, 309, 409, 610
PleI GAGTC 2 cut(s) 555, 651
PpsI GAGTC 2 cut(s) 555, 651
Psp124BI GAGCTC 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 740
PspGI CCWGG 1 cut(s) 740
PspN4I GGNNCC 2 cut(s) 193, 238
PvuII CAGCTG 1 cut(s) 307
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
RseI CAYNNNNRTG 2 cut(s) 221, 628
SacI GAGCTC 1 cut(s) 172
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 5 cut(s) 38, 198, 308, 408, 609
Sau3AI GATC 3 cut(s) 121, 261, 478
SchI GAGTC 2 cut(s) 556, 652
ScrFI CCNGG 2 cut(s) 165, 742
SduI GDGCHC 3 cut(s) 50, 172, 194
SfaNI GCATC 2 cut(s) 142, 754
SmiMI CAYNNNNRTG 2 cut(s) 221, 628
SmlI CTYRAG 2 cut(s) 67, 171
SmoI CTYRAG 2 cut(s) 67, 171
SphI GCATGC 1 cut(s) 280
Sse9I AATT 2 cut(s) 111, 117
SsiI CCGC 4 cut(s) 195, 290, 539, 608
SspMI CTAG 2 cut(s) 564, 656
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 2 cut(s) 163, 740
TaaI ACNGT 4 cut(s) 54, 359, 418, 640
TasI AATT 2 cut(s) 111, 117
TauI GCSGC 1 cut(s) 611
TfiI GAWTC 4 cut(s) 282, 329, 583, 599
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 4 cut(s) 89, 328, 654, 678
TseI GCWGC 4 cut(s) 37, 197, 307, 407
TspDTI ATGAA 5 cut(s) 17, 444, 575, 608, 671
TspRI CASTG 4 cut(s) 89, 328, 654, 678
XceI RCATGY 2 cut(s) 280, 318
XspI CTAG 2 cut(s) 564, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.