pycom11g02790

Cleavage site for pathogenic type III effector avirulence factor Avr

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Forward (+)
2216844 .. 2218239
1396 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 918 bp
ATGATATTGGGATACGTTTTTAGAGGTTCGGGATGTAACGGAGTGCTTGTCTATACTTTTGTTTTTGTAGTTAAGAGAATATGTAATTGCCTCGCAATCATTAGATTTGTAAACTTATTACTTGGGTGCCCTTTGCCTTGCAGGCCACTCCCAAAATTTGGTGAATGGGACGTCAATGATCCTGCTTCGGCTGAGGGGTTTACTGTGATCTTTAACAAGGCTAGGGATGAGAAAAAGACAGGGAGGAGGAGTGGATCCTGGCACTGGCAGGCCTTCGTCTGTAAGATTTTTTCTCATTTAACTGTTGTGCAAGTTATTGCTTTTCAAGTTACGTCAGTAGTACAATATTCAACTATATGTAACACTGCCTTATCTGTCAAACTCAGCGTACCATTTGATGTGAAAATCAATGCAACTGACTTATACAGAACTCTATTAACGAAATGTGAACAGAAGATGATTCGGCTGTTTTATCTGCATTATTCCATTCGAGTGGAATTGGCATGCGTTAACTTAACTCGGGTTCTAATAGAAAGAACTAATGGCCCTGGAAATATTTGCTCTTATCAATCGATCATTGTGTCGGCTTTTCGTTTTCTCTATCTGCTGCATTTCCAATTACTAATAGGCTTGAATTCCTCACTCTTTGCGGATGATTTGACTTGGTTTGATTTCCTGGTGCACAGAAAAAATGGTTTTGCTGCATTCATCAACCACAGCATGGTGTTCTGTAACTGGCTCCACCATATATTTCCAAAGAAGATAGGGAGCTGTATAGCAATGATGGATGGAATTTACCCGGTATTAGAATATACCGATCTGTTTCTTCATCTTATCTTTGCTTCAAGCTTTAGAGCTTTGCTTATGACATTCTTTCCTTCTTCCCTAATTGTATTATCAAACCACTCCAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

306

Amino Acids

34.76

Weight (kDa)

9.16

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 48 - 79 1.1e-18 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 126
AccB7I CCANNNNNTGG 2 cut(s) 158, 721
AciI CCGC 1 cut(s) 650
AclWI GGATC 3 cut(s) 173, 249, 262
AcsI RAATTY 3 cut(s) 155, 634, 792
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 2 cut(s) 342, 390
AfiI CCNNNNNNNGG 3 cut(s) 158, 264, 721
AgsI TTSAA 4 cut(s) 326, 351, 634, 846
AjnI CCWGG 3 cut(s) 257, 547, 675
AleI CACNNNNGTG 1 cut(s) 909
AluBI AGCT 3 cut(s) 771, 849, 857
AluI AGCT 3 cut(s) 771, 849, 857
Alw21I GWGCWC 1 cut(s) 684
Alw44I GTGCAC 1 cut(s) 680
AlwI GGATC 3 cut(s) 173, 249, 262
Ama87I CYCGRG 1 cut(s) 519
AoxI GGCC 3 cut(s) 143, 270, 544
ApaLI GTGCAC 1 cut(s) 680
ApeKI GCWGC 2 cut(s) 607, 701
ApoI RAATTY 3 cut(s) 155, 634, 792
AspS9I GGNCC 1 cut(s) 545
AsuC2I CCSGG 1 cut(s) 800
AsuHPI GGTGA 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 519
BaeGI GKGCMC 2 cut(s) 131, 684
BamHI GGATCC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 684
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 594, 688
BccI CCATC 2 cut(s) 778, 782
BciT130I CCWGG 3 cut(s) 259, 549, 677
BciVI GTATCC 1 cut(s) 5
BcnI CCSGG 1 cut(s) 800
BfaI CTAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 5
BglI GCCNNNNNGGC 1 cut(s) 142
BisI GCNGC 2 cut(s) 608, 702
BlsI GCNGC 2 cut(s) 609, 703
Bme1390I CCNGG 4 cut(s) 259, 549, 677, 800
BmeT110I CYCGRG 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 3 cut(s) 128, 256, 740
BmrFI CCNGG 4 cut(s) 259, 549, 677, 800
BpmI CTGGAG 1 cut(s) 892
Bpu10I CCTNAGC 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 800
Bsa29I ATCGAT 1 cut(s) 572
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 547
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 235, 265
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 264, 721
Bse1I ACTGG 3 cut(s) 269, 740, 909
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 3 cut(s) 259, 549, 677
BseCI ATCGAT 1 cut(s) 572
BseDI CCNNGG 1 cut(s) 547
BseGI GGATG 4 cut(s) 38, 232, 658, 793
BseLI CCNNNNNNNGG 3 cut(s) 158, 264, 721
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 2 cut(s) 183, 397
BseNI ACTGG 3 cut(s) 269, 740, 909
BseRI GAGGAG 2 cut(s) 259, 262
BseSI GKGCMC 2 cut(s) 131, 684
BseXI GCAGC 2 cut(s) 594, 688
BshFI GGCC 3 cut(s) 145, 272, 546
BshNI GGYRCC 1 cut(s) 126
BshVI ATCGAT 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 684
BsiHKCI CYCGRG 1 cut(s) 519
BsiSI CCGG 1 cut(s) 800
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 3 cut(s) 158, 264, 721
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 3 cut(s) 145, 272, 546
BsoBI CYCGRG 1 cut(s) 519
Bsp1286I GDGCHC 2 cut(s) 131, 684
Bsp143I GATC 5 cut(s) 178, 207, 254, 573, 817
BspACI CCGC 1 cut(s) 650
BspANI GGCC 3 cut(s) 145, 272, 546
BspCNI CTCAG 2 cut(s) 184, 396
BspDI ATCGAT 1 cut(s) 572
BspLI GGNNCC 3 cut(s) 128, 256, 740
BspPI GGATC 3 cut(s) 173, 249, 262
BspT107I GGYRCC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 786
BsrI ACTGG 3 cut(s) 269, 740, 909
BssECI CCNNGG 1 cut(s) 547
BssMI GATC 5 cut(s) 178, 207, 254, 573, 817
BssNI GRCGYC 1 cut(s) 171
Bst2UI CCWGG 3 cut(s) 259, 549, 677
Bst4CI ACNGT 2 cut(s) 205, 304
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 143, 270, 505
BstDEI CTNAG 2 cut(s) 192, 383
BstF5I GGATG 4 cut(s) 38, 232, 658, 793
BstKTI GATC 5 cut(s) 181, 210, 257, 576, 820
BstMBI GATC 5 cut(s) 178, 207, 254, 573, 817
BstMWI GCNNNNNNNGC 1 cut(s) 142
BstNI CCWGG 3 cut(s) 259, 549, 677
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 4 cut(s) 257, 547, 675, 798
BstSLI GKGCMC 2 cut(s) 131, 684
BstV1I GCAGC 2 cut(s) 594, 688
BstX2I RGATCY 1 cut(s) 254
BstXI CCANNNNNNTGG 1 cut(s) 493
BstYI RGATCY 1 cut(s) 254
Bsu15I ATCGAT 1 cut(s) 572
BsuI GTATCC 1 cut(s) 5
BsuRI GGCC 3 cut(s) 145, 272, 546
BsuTUI ATCGAT 1 cut(s) 572
BtsCI GGATG 4 cut(s) 38, 232, 658, 793
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 262, 363, 916
Cac8I GCNNGC 3 cut(s) 143, 270, 505
Cfr13I GGNCC 1 cut(s) 545
ClaI ATCGAT 1 cut(s) 572
Csp6I GTAC 2 cut(s) 341, 389
CviAII CATG 2 cut(s) 504, 721
CviQI GTAC 2 cut(s) 341, 389
DdeI CTNAG 2 cut(s) 192, 383
DpnI GATC 5 cut(s) 180, 209, 256, 575, 819
DpnII GATC 5 cut(s) 178, 207, 254, 573, 817
Eco147I AGGCCT 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 519
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 3 cut(s) 257, 547, 675
FaeI CATG 2 cut(s) 507, 724
FaqI GGGAC 1 cut(s) 182
FatI CATG 2 cut(s) 503, 720
Fnu4HI GCNGC 2 cut(s) 608, 702
FokI GGATG 4 cut(s) 45, 239, 665, 800
Fsp4HI GCNGC 2 cut(s) 608, 702
FspBI CTAG 1 cut(s) 222
GluI GCNGC 2 cut(s) 608, 702
GsuI CTGGAG 1 cut(s) 892
HaeIII GGCC 3 cut(s) 145, 272, 546
HapII CCGG 1 cut(s) 800
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 2 cut(s) 507, 724
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 847
HinfI GANTC 1 cut(s) 460
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 1 cut(s) 800
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 5 cut(s) 112, 201, 449, 511, 682
Hpy188III TCNNGA 1 cut(s) 30
Hpy8I GTNNAC 5 cut(s) 112, 201, 449, 511, 682
HpyAV CCTTC 2 cut(s) 283, 888
HpyCH4III ACNGT 2 cut(s) 205, 304
HpyCH4IV ACGT 3 cut(s) 15, 171, 332
HpyCH4V TGCA 7 cut(s) 141, 310, 413, 478, 610, 682, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 142
HpyF3I CTNAG 2 cut(s) 192, 383
HpySE526I ACGT 3 cut(s) 15, 171, 332
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 2 cut(s) 507, 724
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 5 cut(s) 178, 207, 254, 573, 817
LmnI GCTCC 2 cut(s) 744, 768
Lsp1109I GCAGC 2 cut(s) 594, 688
MaeI CTAG 1 cut(s) 222
MaeII ACGT 3 cut(s) 15, 171, 332
MaeIII GTNAC 4 cut(s) 35, 328, 359, 731
MalI GATC 5 cut(s) 180, 209, 256, 575, 819
MboI GATC 5 cut(s) 178, 207, 254, 573, 817
MboII GAAGA 4 cut(s) 466, 772, 818, 873
MflI RGATCY 1 cut(s) 254
MhlI GDGCHC 2 cut(s) 131, 684
MluCI AATT 7 cut(s) 85, 155, 497, 617, 634, 792, 888
MnlI CCTC 6 cut(s) 17, 101, 187, 237, 240, 649
MseI TTAA 6 cut(s) 72, 213, 299, 437, 510, 515
MslI CAYNNNNRTG 2 cut(s) 491, 909
MspI CCGG 1 cut(s) 800
MspR9I CCNGG 4 cut(s) 259, 549, 677, 800
Mva1269I GAATGC 1 cut(s) 704
MvaI CCWGG 3 cut(s) 259, 549, 677
MwoI GCNNNNNNNGC 1 cut(s) 142
NciI CCSGG 1 cut(s) 800
NdeII GATC 5 cut(s) 178, 207, 254, 573, 817
NlaIII CATG 2 cut(s) 507, 724
NlaIV GGNNCC 3 cut(s) 128, 256, 740
NspI RCATGY 1 cut(s) 507
OliI CACNNNNGTG 1 cut(s) 909
PaeI GCATGC 1 cut(s) 507
PceI AGGCCT 1 cut(s) 272
PctI GAATGC 1 cut(s) 704
PfeI GAWTC 1 cut(s) 460
PflMI CCANNNNNTGG 2 cut(s) 158, 721
PkrI GCNGC 2 cut(s) 609, 703
Psp6I CCWGG 3 cut(s) 257, 547, 675
PspGI CCWGG 3 cut(s) 257, 547, 675
PspN4I GGNNCC 3 cut(s) 128, 256, 740
PspPI GGNCC 1 cut(s) 545
PsuI RGATCY 1 cut(s) 254
RsaI GTAC 2 cut(s) 342, 390
RsaNI GTAC 2 cut(s) 341, 389
RseI CAYNNNNRTG 2 cut(s) 491, 909
SaqAI TTAA 6 cut(s) 72, 213, 299, 437, 510, 515
SatI GCNGC 2 cut(s) 608, 702
Sau3AI GATC 5 cut(s) 178, 207, 254, 573, 817
Sau96I GGNCC 1 cut(s) 545
ScrFI CCNGG 4 cut(s) 259, 549, 677, 800
SduI GDGCHC 2 cut(s) 131, 684
SetI ASST 7 cut(s) 18, 28, 174, 335, 773, 851, 859
SmiMI CAYNNNNRTG 2 cut(s) 491, 909
SphI GCATGC 1 cut(s) 507
Sse9I AATT 7 cut(s) 85, 155, 497, 617, 634, 792, 888
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 1 cut(s) 650
SspI AATATT 2 cut(s) 347, 556
SspMI CTAG 1 cut(s) 222
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 4 cut(s) 257, 547, 675, 798
TaaI ACNGT 2 cut(s) 205, 304
TaiI ACGT 3 cut(s) 18, 174, 335
TaqI TCGA 2 cut(s) 490, 572
TasI AATT 7 cut(s) 85, 155, 497, 617, 634, 792, 888
TatI WGTACW 1 cut(s) 340
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 6 cut(s) 72, 213, 299, 437, 510, 515
Tru9I TTAA 6 cut(s) 72, 213, 299, 437, 510, 515
TscAI CASTG 3 cut(s) 269, 370, 916
TseI GCWGC 2 cut(s) 607, 701
TspDTI ATGAA 2 cut(s) 697, 818
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 3 cut(s) 269, 370, 916
Van91I CCANNNNNTGG 2 cut(s) 158, 721
VneI GTGCAC 1 cut(s) 680
XapI RAATTY 3 cut(s) 155, 634, 792
XceI RCATGY 1 cut(s) 507
XspI CTAG 1 cut(s) 222
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.