pycom11g04850

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Reverse (-)
3749127 .. 3749994
868 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGGTCAAAAGGCCTAATCTGTTGTGGCTTATGCATACAGTCCGTCCTTGGACCGAGCTCATCGACCGCACTGCCTTCACCAAGCTGACTTTGCTTACCGATGTTGCCTTCTGCATCTGCAAAAATGCCGTCTACTTCTGCGTCAACTACCTCATCATACTCGTAGCCGTACTCGCCTACTCCCTCATCTCAAACCCCTTCTCTATTATCATCCTCGCCTTTCTCACCAGAGCATGGATCTTCCTCTACTCGCGCCGATTGTCGGAGGAACCGCTCATGATCCTCGACCGCACTGTCTTAGAAACCCAAGCGATGTTCGGGCTGGGCGTGGCGATGCTGATCATGATCTTAGTAACGAGTGTGATGTTGCTACTGCTAACGGCGGTCATGGTGAGGGCTGGGATCATCTGTGTTCACGGAGCATTTAGAGATCCCGATCGATCTGCTTCTGGAGGAAACGCAGCCGTTGGGTTCTGGAATCGCTTCGATTTTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

166

Amino Acids

18.71

Weight (kDa)

9.33

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 12 - 147 5.2e-30 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 234
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 132
AccII CGCG 1 cut(s) 253
AciI CCGC 4 cut(s) 67, 272, 289, 383
AclWI GGATC 4 cut(s) 245, 274, 410, 425
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 2 cut(s) 234, 262
AluBI AGCT 2 cut(s) 58, 85
AluI AGCT 2 cut(s) 58, 85
Alw21I GWGCWC 1 cut(s) 60
AlwI GGATC 4 cut(s) 245, 274, 410, 425
AoxI GGCC 1 cut(s) 11
ApeKI GCWGC 1 cut(s) 461
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
Asp700I GAANNNNTTC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 3 cut(s) 70, 217, 403
AvaII GGWCC 1 cut(s) 51
BanII GRGCYC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 473
BceAI ACGGC 4 cut(s) 113, 152, 396, 449
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 425, 459
BclI TGATCA 1 cut(s) 339
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 270
BmsI GCATC 2 cut(s) 123, 324
BpmI CTGGAG 1 cut(s) 471
Bsa29I ATCGAT 1 cut(s) 439
BsaBI GATNNNNATC 3 cut(s) 338, 344, 435
BsaJI CCNNGG 1 cut(s) 47
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 262
Bse8I GATNNNNATC 3 cut(s) 338, 344, 435
BseCI ATCGAT 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 1 cut(s) 210
BseJI GATNNNNATC 3 cut(s) 338, 344, 435
BseLI CCNNNNNNNGG 2 cut(s) 234, 262
BseXI GCAGC 1 cut(s) 473
BseYI CCCAGC 2 cut(s) 322, 398
Bsh1236I CGCG 1 cut(s) 253
Bsh1285I CGRYCG 3 cut(s) 67, 289, 439
BshFI GGCC 1 cut(s) 13
BshVI ATCGAT 1 cut(s) 439
BsiEI CGRYCG 3 cut(s) 67, 289, 439
BsiHKAI GWGCWC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 234, 262
BsnI GGCC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
BspACI CCGC 4 cut(s) 67, 272, 289, 383
BspANI GGCC 1 cut(s) 13
BspDI ATCGAT 1 cut(s) 439
BspFNI CGCG 1 cut(s) 253
BspHI TCATGA 2 cut(s) 276, 342
BspLI GGNNCC 1 cut(s) 270
BspPI GGATC 4 cut(s) 245, 274, 410, 425
BsrBI CCGCTC 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
BssT1I CCWWGG 1 cut(s) 47
Bst4CI ACNGT 2 cut(s) 40, 295
BstDEI CTNAG 2 cut(s) 298, 349
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 1 cut(s) 253
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 8 cut(s) 240, 282, 342, 348, 405, 433, 439, 443
BstMBI GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
BstMCI CGRYCG 3 cut(s) 67, 289, 439
BstMWI GCNNNNNNNGC 2 cut(s) 91, 173
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 473
BstX2I RGATCY 2 cut(s) 237, 430
BstYI RGATCY 2 cut(s) 237, 430
Bsu15I ATCGAT 1 cut(s) 439
BsuRI GGCC 1 cut(s) 13
BsuTUI ATCGAT 1 cut(s) 439
BtgZI GCGATG 2 cut(s) 326, 347
BtsCI GGATG 1 cut(s) 210
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 291
CciI TCATGA 2 cut(s) 276, 342
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 51
ClaI ATCGAT 1 cut(s) 439
CseI GACGC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 170
CviAII CATG 4 cut(s) 234, 277, 343, 388
CviJI RGCY 8 cut(s) 13, 28, 58, 85, 167, 322, 398, 464
CviKI_1 RGCY 8 cut(s) 13, 28, 58, 85, 167, 322, 398, 464
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 2 cut(s) 298, 349
DpnI GATC 8 cut(s) 239, 281, 341, 347, 404, 432, 438, 442
DpnII GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 13
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 47
EcoT22I ATGCAT 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 4 cut(s) 237, 280, 346, 391
FaiI YATR 7 cut(s) 32, 36, 158, 235, 278, 344, 389
FatI CATG 4 cut(s) 233, 276, 342, 387
FbaI TGATCA 1 cut(s) 339
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 1 cut(s) 462
GlaI GCGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 462
GsaI CCCAGC 2 cut(s) 326, 402
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 1 cut(s) 13
HgaI GACGC 1 cut(s) 130
HhaI GCGC 1 cut(s) 255
Hin1II CATG 4 cut(s) 237, 280, 346, 391
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 1 cut(s) 478
HphI GGTGA 3 cut(s) 70, 217, 403
Hpy166II GTNNAC 3 cut(s) 133, 145, 415
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 5 cut(s) 277, 343, 434, 450, 475
Hpy8I GTNNAC 3 cut(s) 133, 145, 415
HpyAV CCTTC 3 cut(s) 85, 118, 208
HpyCH4III ACNGT 2 cut(s) 40, 295
HpyCH4V TGCA 3 cut(s) 34, 114, 120
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 173
HpyF3I CTNAG 2 cut(s) 298, 349
Hsp92II CATG 4 cut(s) 237, 280, 346, 391
HspAI GCGC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 339
Kzo9I GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 5 cut(s) 241, 308, 384, 435, 460
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 2 cut(s) 123, 324
MaeIII GTNAC 1 cut(s) 352
MalI GATC 8 cut(s) 239, 281, 341, 347, 404, 432, 438, 442
MbiI CCGCTC 1 cut(s) 274
MboI GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
MboII GAAGA 1 cut(s) 232
MflI RGATCY 2 cut(s) 237, 430
MhlI GDGCHC 1 cut(s) 60
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 8 cut(s) 161, 194, 224, 254, 259, 293, 387, 446
Mph1103I ATGCAT 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 482
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 2 cut(s) 91, 173
NdeII GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
NlaIII CATG 4 cut(s) 237, 280, 346, 391
NlaIV GGNNCC 1 cut(s) 270
NsiI ATGCAT 1 cut(s) 36
PagI TCATGA 2 cut(s) 276, 342
PceI AGGCCT 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 478
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 1 cut(s) 463
Ple19I CGATCG 1 cut(s) 439
Psp124BI GAGCTC 1 cut(s) 60
PspFI CCCAGC 2 cut(s) 322, 398
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 51
PsuI RGATCY 2 cut(s) 237, 430
PvuI CGATCG 1 cut(s) 439
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
SacI GAGCTC 1 cut(s) 60
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 8 cut(s) 237, 279, 339, 345, 402, 430, 436, 440
Sau96I GGNCC 1 cut(s) 51
SduI GDGCHC 1 cut(s) 60
SetI ASST 3 cut(s) 60, 87, 153
SfaNI GCATC 2 cut(s) 123, 324
SinI GGWCC 1 cut(s) 51
SseBI AGGCCT 1 cut(s) 13
SsiI CCGC 4 cut(s) 67, 272, 289, 383
SstI GAGCTC 1 cut(s) 60
StuI AGGCCT 1 cut(s) 13
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 2 cut(s) 40, 295
TaqI TCGA 4 cut(s) 63, 285, 439, 486
TaqII GACCGA 1 cut(s) 68
TfiI GAWTC 1 cut(s) 478
TscAI CASTG 2 cut(s) 76, 298
TseI GCWGC 1 cut(s) 461
TspGWI ACGGA 2 cut(s) 32, 432
TspRI CASTG 2 cut(s) 76, 298
Van91I CCANNNNNTGG 1 cut(s) 234
VpaK11BI GGWCC 1 cut(s) 51
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.