pycom11g07850

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Forward (+)
5978356 .. 5979889
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGGTCTCCACCGAAGATCGATCTTTCTCGAGCGCCCCAGGGCTCCTGGCTGGTAGCAAAGCAGAAGAACCAAAGGCTGGAGGAGACCCTCTGTCTCAACTCACAAAAGGAGGGGCTGCTCTCAAACTTTGTAGGACTTGTGGTAAGAAGGGTGATCACTGGACTTCACATTGCCCGTATAAGGATCTTGCCCCACCGACTGAAGGATTTATTGATAAACCTCCTACAACAGAAGCAGTGCCTGGAACTCCTAGCACAGGTAAGTATGTTCCTCCAGTCCAGAGAGGAGGTGGAGACAGAGTTGGAAGTGATATGAGACGAAGGAATGATGAGAACTCTGTTCGTGTCACCAACCTTTCAGAGGACACTCGTGAGCCTGACTTGCTTGAACTTTTCCGGCCTTTTGGTGCTGTTAGTCGTGTTTACGTTGCTTTGGATAAGAATACTTCCGTTAGCAGAGGATTTGGTTTCGTCAACTTTGTGAACAGGGAAGATGCCCAGAGAGCAATCAACAAACTGAACGGGTACGGTTATGACAATCTCATCCTCAGGGTTGAATGGGCCACACCGAGGACAAATTAG

Protein Analysis

194

Amino Acids

21.01

Weight (kDa)

8.77

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 20 - 63 1.1e-08 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 114 - 180 6.2e-20 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 77
AclWI GGATC 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 1 cut(s) 527
AfiI CCNNNNNNNGG 6 cut(s) 77, 181, 203, 257, 361, 570
AgsI TTSAA 2 cut(s) 389, 557
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 3 cut(s) 37, 45, 241
Alw26I GTCTC 5 cut(s) 10, 78, 99, 288, 310
AlwI GGATC 1 cut(s) 192
AlwNI CAGNNNCTG 1 cut(s) 242
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 2 cut(s) 398, 561
ApeKI GCWGC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 561
AsuHPI GGTGA 2 cut(s) 164, 340
AvaI CYCGRG 1 cut(s) 28
AxyI CCTNAGG 1 cut(s) 548
BanII GRGCYC 1 cut(s) 45
BauI CACGAG 1 cut(s) 369
BbvI GCAGC 1 cut(s) 103
BciT130I CCWGG 3 cut(s) 39, 47, 243
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 5 cut(s) 10, 78, 99, 288, 310
BfaI CTAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 36
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 3 cut(s) 39, 47, 243
BmeRI GACNNNNNGTC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 561
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 3 cut(s) 39, 47, 243
BmsI GCATC 1 cut(s) 484
BpmI CTGGAG 2 cut(s) 99, 258
Bsa29I ATCGAT 1 cut(s) 19
BsaI GGTCTC 2 cut(s) 10, 78
BsaJI CCNNGG 3 cut(s) 37, 38, 569
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 6 cut(s) 77, 181, 203, 257, 361, 570
Bse1I ACTGG 2 cut(s) 164, 275
Bse21I CCTNAGG 1 cut(s) 548
Bse3DI GCAATG 1 cut(s) 169
BseBI CCWGG 3 cut(s) 39, 47, 243
BseCI ATCGAT 1 cut(s) 19
BseDI CCNNGG 3 cut(s) 37, 38, 569
BseGI GGATG 1 cut(s) 543
BseLI CCNNNNNNNGG 6 cut(s) 77, 181, 203, 257, 361, 570
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 562
BseNI ACTGG 2 cut(s) 164, 275
BseRI GAGGAG 2 cut(s) 96, 300
BseXI GCAGC 1 cut(s) 103
BshFI GGCC 2 cut(s) 400, 563
BshVI ATCGAT 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 1 cut(s) 397
BslI CCNNNNNNNGG 6 cut(s) 77, 181, 203, 257, 361, 570
BsmAI GTCTC 5 cut(s) 10, 78, 99, 288, 310
BsmBI CGTCTC 1 cut(s) 310
BsnI GGCC 2 cut(s) 400, 563
Bso31I GGTCTC 2 cut(s) 10, 78
BsoBI CYCGRG 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 4 cut(s) 16, 20, 154, 184
BspANI GGCC 2 cut(s) 400, 563
BspCNI CTCAG 1 cut(s) 561
BspDI ATCGAT 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 1 cut(s) 192
BspTNI GGTCTC 2 cut(s) 10, 78
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 2 cut(s) 164, 275
BssECI CCNNGG 3 cut(s) 37, 38, 569
BssMI GATC 4 cut(s) 16, 20, 154, 184
BssSI CACGAG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 3 cut(s) 39, 47, 243
Bst4CI ACNGT 1 cut(s) 530
BstDEI CTNAG 1 cut(s) 548
BstENI CCTNNNNNAGG 2 cut(s) 255, 359
BstF5I GGATG 1 cut(s) 543
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 4 cut(s) 19, 23, 157, 187
BstMAI GTCTC 5 cut(s) 10, 78, 99, 288, 310
BstMBI GATC 4 cut(s) 16, 20, 154, 184
BstMWI GCNNNNNNNGC 2 cut(s) 382, 503
BstNI CCWGG 3 cut(s) 39, 47, 243
BstSCI CCNGG 3 cut(s) 37, 45, 241
BstV1I GCAGC 1 cut(s) 103
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 19
Bsu36I CCTNAGG 1 cut(s) 548
BsuRI GGCC 2 cut(s) 400, 563
BsuTUI ATCGAT 1 cut(s) 19
BtsCI GGATG 1 cut(s) 543
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 157, 243
CaiI CAGNNNCTG 1 cut(s) 242
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 561
ClaI ATCGAT 1 cut(s) 19
Csp6I GTAC 1 cut(s) 526
CviJI RGCY 7 cut(s) 43, 50, 77, 116, 376, 400, 563
CviKI_1 RGCY 7 cut(s) 43, 50, 77, 116, 376, 400, 563
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 1 cut(s) 548
DpnI GATC 4 cut(s) 18, 22, 156, 186
DpnII GATC 4 cut(s) 16, 20, 154, 184
DriI GACNNNNNGTC 1 cut(s) 91
Eam1105I GACNNNNNGTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 45
Eco31I GGTCTC 2 cut(s) 10, 78
Eco57I CTGAAG 1 cut(s) 222
Eco81I CCTNAGG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 28
EcoNI CCTNNNNNAGG 2 cut(s) 255, 359
EcoRII CCWGG 3 cut(s) 37, 45, 241
EcoT38I GRGCYC 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 310
FaiI YATR 4 cut(s) 180, 267, 314, 534
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 530
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 117
GsuI CTGGAG 2 cut(s) 99, 258
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 2 cut(s) 400, 563
HapII CCGG 1 cut(s) 397
HhaI GCGC 1 cut(s) 35
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HpaII CCGG 1 cut(s) 397
HphI GGTGA 2 cut(s) 164, 340
Hpy166II GTNNAC 3 cut(s) 424, 475, 484
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 3 cut(s) 28, 280, 371
Hpy8I GTNNAC 3 cut(s) 424, 475, 484
HpyAV CCTTC 3 cut(s) 142, 197, 315
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 503
HpyF3I CTNAG 1 cut(s) 548
HpySE526I ACGT 1 cut(s) 426
HspAI GCGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 4 cut(s) 16, 20, 154, 184
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 1 cut(s) 484
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 1 cut(s) 346
MalI GATC 4 cut(s) 18, 22, 156, 186
MboI GATC 4 cut(s) 16, 20, 154, 184
MboII GAAGA 3 cut(s) 26, 77, 503
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 1 cut(s) 577
MmeI TCCRAC 1 cut(s) 283
MspI CCGG 1 cut(s) 397
MspR9I CCNGG 3 cut(s) 39, 47, 243
MvaI CCWGG 3 cut(s) 39, 47, 243
MwoI GCNNNNNNNGC 2 cut(s) 382, 503
NdeII GATC 4 cut(s) 16, 20, 154, 184
NlaIV GGNNCC 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 28
PasI CCCWGGG 1 cut(s) 38
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 1 cut(s) 118
Psp6I CCWGG 3 cut(s) 37, 45, 241
PspGI CCWGG 3 cut(s) 37, 45, 241
PspN4I GGNNCC 1 cut(s) 44
PspPI GGNCC 1 cut(s) 561
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 4 cut(s) 16, 20, 154, 184
Sau96I GGNCC 1 cut(s) 561
ScrFI CCNGG 3 cut(s) 39, 47, 243
SduI GDGCHC 1 cut(s) 45
SetI ASST 5 cut(s) 223, 262, 292, 357, 429
SfaNI GCATC 1 cut(s) 484
Sfr274I CTCGAG 1 cut(s) 28
SlaI CTCGAG 1 cut(s) 28
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 1 cut(s) 577
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 37, 45, 241
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 1 cut(s) 429
TaqI TCGA 2 cut(s) 19, 29
TasI AATT 1 cut(s) 577
TscAI CASTG 2 cut(s) 164, 243
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 346
TspGWI ACGGA 1 cut(s) 439
TspRI CASTG 2 cut(s) 164, 243
Van91I CCANNNNNTGG 1 cut(s) 77
XagI CCTNNNNNAGG 2 cut(s) 255, 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XhoI CTCGAG 1 cut(s) 28
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.