pycom11g08680

serine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Forward (+)
6791546 .. 6793293
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
ATGTCAACTGGGCTTCAAATTGCAATGATAATAAATCAGAATGCAAAATCCCCAAATTTAACTGAAAACCGCAGCTCAGAAGGAGCTAAAAGATTACCTTGCCATCGACGACGAGGCAGCAGTCCTTGCGATCATTGTGAGTGGAGACCTCAGCCAAAGTGTAGACCCTTTTTAAGGGCCAGATCCCGGACTTGTGGATTTGGTGGAAGGGATCCGGAGTCCTTTGCGTTGAAAATCTCATCTCCTCTCCTTTCAGCCCTTCAAGGAAAACCCACTTCTTGGGCCGTCTTCAACCACGTTAGAAAAGATGGGTGCTCTAGATGTGCAGAGAGTGGTTCATCAACACCAGGGATGGCGGATGATCTCATGTATGTTTTTTCCATGTGCTTGCCAATATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

134

Amino Acids

14.65

Weight (kDa)

9.42

Isoelectric Point (pI)

73.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 279
AccI GTMKAC 1 cut(s) 163
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 2 cut(s) 70, 356
AclWI GGATC 3 cut(s) 177, 206, 219
AcsI RAATTY 1 cut(s) 55
AfiI CCNNNNNNNGG 3 cut(s) 174, 186, 279
AgsI TTSAA 4 cut(s) 17, 232, 263, 292
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 2 cut(s) 75, 86
AluI AGCT 2 cut(s) 75, 86
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 3 cut(s) 177, 206, 219
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 2 cut(s) 177, 282
ApeKI GCWGC 2 cut(s) 72, 117
ApoI RAATTY 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 177, 282
AsuC2I CCSGG 1 cut(s) 187
BamHI GGATCC 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 84, 129
BccI CCATC 3 cut(s) 111, 302, 346
BceAI ACGGC 1 cut(s) 269
BciT130I CCWGG 1 cut(s) 348
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 318
BisI GCNGC 2 cut(s) 73, 118
BlsI GCNGC 2 cut(s) 74, 119
Bme1390I CCNGG 2 cut(s) 187, 348
BmgT120I GGNCC 2 cut(s) 177, 282
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 2 cut(s) 187, 348
BmrI ACTGGG 1 cut(s) 18
BmuI ACTGGG 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 347
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 186, 279
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 1 cut(s) 30
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 2 cut(s) 357, 364
BseLI CCNNNNNNNGG 3 cut(s) 174, 186, 279
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 2 cut(s) 90, 164
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 84, 129
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 2 cut(s) 179, 284
BsiHKAI GWGCWC 1 cut(s) 317
BsiSI CCGG 2 cut(s) 187, 215
BslI CCNNNNNNNGG 3 cut(s) 174, 186, 279
BsmAI GTCTC 1 cut(s) 139
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 2 cut(s) 179, 284
Bso31I GGTCTC 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 317
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 4 cut(s) 130, 182, 211, 361
BspACI CCGC 2 cut(s) 70, 356
BspANI GGCC 2 cut(s) 179, 284
BspCNI CTCAG 2 cut(s) 89, 163
BspEI TCCGGA 1 cut(s) 214
BspLI GGNNCC 1 cut(s) 213
BspPI GGATC 3 cut(s) 177, 206, 219
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 4 cut(s) 130, 182, 211, 361
Bst2UI CCWGG 1 cut(s) 348
BstAPI GCANNNNNTGC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 76, 150
BstENI CCTNNNNNAGG 1 cut(s) 172
BstF5I GGATG 2 cut(s) 357, 364
BstKTI GATC 4 cut(s) 133, 185, 214, 364
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 4 cut(s) 130, 182, 211, 361
BstMWI GCNNNNNNNGC 1 cut(s) 126
BstNI CCWGG 1 cut(s) 348
BstSCI CCNGG 2 cut(s) 185, 346
BstV1I GCAGC 2 cut(s) 84, 129
BstV2I GAAGAC 1 cut(s) 280
BstX2I RGATCY 2 cut(s) 182, 211
BstYI RGATCY 2 cut(s) 182, 211
BsuRI GGCC 2 cut(s) 179, 284
BtsCI GGATG 2 cut(s) 357, 364
Cac8I GCNNGC 1 cut(s) 389
Cfr13I GGNCC 2 cut(s) 177, 282
CviAII CATG 2 cut(s) 367, 382
CviJI RGCY 7 cut(s) 13, 75, 86, 154, 179, 257, 284
CviKI_1 RGCY 7 cut(s) 13, 75, 86, 154, 179, 257, 284
DdeI CTNAG 2 cut(s) 76, 150
DpnI GATC 4 cut(s) 132, 184, 213, 363
DpnII GATC 4 cut(s) 130, 182, 211, 361
EciI GGCGGA 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 139
EcoNI CCTNNNNNAGG 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 346
FaeI CATG 2 cut(s) 370, 385
FaiI YATR 4 cut(s) 368, 372, 383, 397
FalI AAGNNNNNCTT 2 cut(s) 82, 114
FatI CATG 2 cut(s) 366, 381
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 2 cut(s) 73, 118
FokI GGATG 2 cut(s) 364, 371
Fsp4HI GCNGC 2 cut(s) 73, 118
FspBI CTAG 1 cut(s) 318
GluI GCNGC 2 cut(s) 73, 118
HaeIII GGCC 2 cut(s) 179, 284
HapII CCGG 2 cut(s) 187, 215
Hin1II CATG 2 cut(s) 370, 385
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 218
HpaII CCGG 2 cut(s) 187, 215
Hpy166II GTNNAC 2 cut(s) 6, 164
Hpy188I TCNGA 2 cut(s) 39, 79
Hpy188III TCNNGA 2 cut(s) 215, 318
Hpy8I GTNNAC 2 cut(s) 6, 164
Hpy99I CGWCG 2 cut(s) 111, 114
HpyAV CCTTC 3 cut(s) 74, 201, 269
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 3 cut(s) 23, 44, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 126
HpyF3I CTNAG 2 cut(s) 76, 150
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 2 cut(s) 370, 385
Kpn2I TCCGGA 1 cut(s) 214
Kzo9I GATC 4 cut(s) 130, 182, 211, 361
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 5 cut(s) 193, 200, 228, 333, 360
Lsp1109I GCAGC 2 cut(s) 84, 129
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 297
MalI GATC 4 cut(s) 132, 184, 213, 363
MboI GATC 4 cut(s) 130, 182, 211, 361
MboII GAAGA 1 cut(s) 280
MflI RGATCY 2 cut(s) 182, 211
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 2 cut(s) 18, 55
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 3 cut(s) 107, 159, 255
MroI TCCGGA 1 cut(s) 214
MseI TTAA 2 cut(s) 59, 173
MspI CCGG 2 cut(s) 187, 215
MspR9I CCNGG 2 cut(s) 187, 348
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 126
NciI CCSGG 1 cut(s) 187
NdeII GATC 4 cut(s) 130, 182, 211, 361
NlaIII CATG 2 cut(s) 370, 385
NlaIV GGNNCC 1 cut(s) 213
PctI GAATGC 1 cut(s) 46
PflMI CCANNNNNTGG 1 cut(s) 279
PfoI TCCNGGA 1 cut(s) 185
PkrI GCNGC 2 cut(s) 74, 119
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 2 cut(s) 177, 282
PsuI RGATCY 2 cut(s) 182, 211
SaqAI TTAA 2 cut(s) 59, 173
SatI GCNGC 2 cut(s) 73, 118
Sau3AI GATC 4 cut(s) 130, 182, 211, 361
Sau96I GGNCC 2 cut(s) 177, 282
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 187, 348
SduI GDGCHC 1 cut(s) 317
SetI ASST 5 cut(s) 77, 88, 100, 151, 300
Sse9I AATT 2 cut(s) 18, 55
SsiI CCGC 2 cut(s) 70, 356
SspMI CTAG 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 185, 346
TaiI ACGT 1 cut(s) 300
TaqI TCGA 1 cut(s) 106
TasI AATT 2 cut(s) 18, 55
Tru1I TTAA 2 cut(s) 59, 173
Tru9I TTAA 2 cut(s) 59, 173
TseI GCWGC 2 cut(s) 72, 117
TspDTI ATGAA 1 cut(s) 327
Van91I CCANNNNNTGG 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 172
XapI RAATTY 1 cut(s) 55
XbaI TCTAGA 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.