pycom11g09410

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Reverse (-)
7638814 .. 7639731
918 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGCAACAAGTATCCACCGTCCAATTCCACTTAAACTCACCGATCCGCGATCCTCGTGAAATCCCAGCAGCCAATAGAATCCAACCGCTCACCATATATAAAAACACAATCTCCTCATACTTCGGTCATCACAAAAATATCCAACTCTCAGTCTCAATCCAAATCGAAAAAGCCCCAAACTTTTCTCTCCCAACTCTATCAATGGCGCCCAAAGCCGAGAAGAAGCCTGCGGAGAAGAAGCCAGCAGAGGAGAAGAAGTCTGCCGTGGCGGAGAAAGCCCCCGCCGAGAAGAAGCCCAAGGCCGGGAAGAAGCTCCCCAAGGAAGCCGGGGCCGCCGCCGGCGACAAGAAGAAGAAGAGATCGAAGAAGAACGTGGAGACCTACAAGATCTACATCTTCAAGGTCCTGAAGCAGGTCCACCCTGACATCGGAATCTCCAGCAAGGCCATGGGAATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTAGCCCAGGAGTCGTCCAGGCTCGCCAGGTACAACAAAAAGCCCACCATCACTTCTCGGGAGATCCAGACCGCCGTGAGGCTCGTGCTTCCCGGCGAGCTCGCCAAGCACGCCGTTTCGGAAGGGACAAAGGCCGTGACTAAGTGCATTTTTCTTTCTATTTTTTGTGACTTTGTGTGGATTTGGAACTCGATCTTTATCTTGGTAGGAAATGAAGTATTATGGTTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

241

Amino Acids

26.92

Weight (kDa)

9.92

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 115 - 191 3.7e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 145 - 210 7.1e-07 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 205
AccBSI CCGCTC 1 cut(s) 88
AccII CGCG 1 cut(s) 48
AciI CCGC 8 cut(s) 46, 86, 230, 269, 282, 333, 336, 561
AclWI GGATC 3 cut(s) 37, 44, 547
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 1 cut(s) 521
AfiI CCNNNNNNNGG 5 cut(s) 302, 303, 412, 428, 567
AgsI TTSAA 1 cut(s) 400
AjnI CCWGG 3 cut(s) 495, 506, 515
AluBI AGCT 4 cut(s) 313, 465, 490, 589
AluI AGCT 4 cut(s) 313, 465, 490, 589
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 157, 371
AlwI GGATC 3 cut(s) 37, 44, 547
Ama87I CYCGRG 1 cut(s) 546
AoxI GGCC 4 cut(s) 300, 330, 444, 621
ApeKI GCWGC 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 464
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 3 cut(s) 330, 403, 415
AsuC2I CCSGG 3 cut(s) 304, 328, 582
AsuHPI GGTGA 2 cut(s) 30, 82
AsuNHI GCTAGC 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 2 cut(s) 403, 415
BanI GGYRCC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 591
BauI CACGAG 2 cut(s) 54, 572
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 80
BccI CCATC 1 cut(s) 545
BceAI ACGGC 4 cut(s) 248, 548, 587, 608
BciT130I CCWGG 3 cut(s) 497, 508, 517
BciVI GTATCC 1 cut(s) 22
BcnI CCSGG 3 cut(s) 304, 328, 582
BcoDI GTCTC 2 cut(s) 157, 371
BfaI CTAG 1 cut(s) 491
BfoI RGCGCY 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 403
BfuI GTATCC 1 cut(s) 22
BglII AGATCT 1 cut(s) 387
BisI GCNGC 3 cut(s) 69, 333, 336
BlsI GCNGC 3 cut(s) 70, 334, 337
Bme1390I CCNGG 6 cut(s) 304, 328, 497, 508, 517, 582
Bme18I GGWCC 2 cut(s) 403, 415
BmeT110I CYCGRG 1 cut(s) 546
BmgT120I GGNCC 3 cut(s) 330, 403, 415
BmiI GGNNCC 2 cut(s) 207, 331
BmrFI CCNGG 6 cut(s) 304, 328, 497, 508, 517, 582
BmtI GCTAGC 1 cut(s) 494
BpmI CTGGAG 1 cut(s) 421
BpuMI CCSGG 3 cut(s) 304, 328, 582
BsaBI GATNNNNATC 2 cut(s) 392, 686
BsaHI GRCGYC 1 cut(s) 206
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 6 cut(s) 264, 297, 318, 327, 447, 495
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 242, 272
Bsc4I CCNNNNNNNGG 5 cut(s) 302, 303, 412, 428, 567
Bse118I RCCGGY 1 cut(s) 338
Bse8I GATNNNNATC 2 cut(s) 392, 686
BseBI CCWGG 3 cut(s) 497, 508, 517
BseDI CCNNGG 6 cut(s) 264, 297, 318, 327, 447, 495
BseJI GATNNNNATC 2 cut(s) 392, 686
BseLI CCNNNNNNNGG 5 cut(s) 302, 303, 412, 428, 567
BseMII CTCAG 1 cut(s) 162
BseRI GAGGAG 2 cut(s) 103, 263
BseXI GCAGC 1 cut(s) 80
BseYI CCCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 4 cut(s) 302, 332, 446, 623
BshNI GGYRCC 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 4 cut(s) 303, 327, 339, 582
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 5 cut(s) 302, 303, 412, 428, 567
BsmAI GTCTC 2 cut(s) 157, 371
BsmFI GGGAC 1 cut(s) 628
BsnI GGCC 4 cut(s) 302, 332, 446, 623
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 546
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 6 cut(s) 42, 49, 359, 387, 552, 681
Bsp19I CCATGG 1 cut(s) 447
BspACI CCGC 8 cut(s) 46, 86, 230, 269, 282, 333, 336, 561
BspANI GGCC 4 cut(s) 302, 332, 446, 623
BspCNI CTCAG 1 cut(s) 161
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 207, 331
BspMI ACCTGC 1 cut(s) 403
BspOI GCTAGC 1 cut(s) 494
BspPI GGATC 3 cut(s) 37, 44, 547
BspT107I GGYRCC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 338
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 6 cut(s) 264, 297, 318, 327, 447, 495
BssMI GATC 6 cut(s) 42, 49, 359, 387, 552, 681
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 2 cut(s) 54, 572
BssT1I CCWWGG 3 cut(s) 297, 318, 447
Bst2BI CACGAG 2 cut(s) 54, 572
Bst2UI CCWGG 3 cut(s) 497, 508, 517
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 350
BstACI GRCGYC 1 cut(s) 206
BstC8I GCNNGC 8 cut(s) 228, 243, 340, 492, 513, 587, 591, 600
BstDEI CTNAG 2 cut(s) 148, 630
BstDSI CCRYGG 2 cut(s) 264, 447
BstENI CCTNNNNNAGG 1 cut(s) 410
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 209
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 6 cut(s) 45, 52, 362, 390, 555, 684
BstMAI GTCTC 2 cut(s) 157, 371
BstMBI GATC 6 cut(s) 42, 49, 359, 387, 552, 681
BstMWI GCNNNNNNNGC 5 cut(s) 212, 275, 332, 595, 599
BstNI CCWGG 3 cut(s) 497, 508, 517
BstSCI CCNGG 6 cut(s) 302, 326, 495, 506, 515, 580
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 80
BstX2I RGATCY 2 cut(s) 387, 552
BstYI RGATCY 2 cut(s) 387, 552
BsuI GTATCC 1 cut(s) 22
BsuRI GGCC 4 cut(s) 302, 332, 446, 623
BtgI CCRYGG 2 cut(s) 264, 447
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 8 cut(s) 228, 243, 340, 492, 513, 587, 591, 600
CciI TCATGA 1 cut(s) 456
CfoI GCGC 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 3 cut(s) 330, 403, 415
Csp6I GTAC 1 cut(s) 520
CviAII CATG 2 cut(s) 448, 457
CviQI GTAC 1 cut(s) 520
DdeI CTNAG 2 cut(s) 148, 630
DinI GGCGCC 1 cut(s) 207
DpnI GATC 6 cut(s) 44, 51, 361, 389, 554, 683
DpnII GATC 6 cut(s) 42, 49, 359, 387, 552, 681
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 589
Eco130I CCWWGG 3 cut(s) 297, 318, 447
Eco24I GRGCYC 1 cut(s) 591
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 2 cut(s) 403, 415
Eco53kI GAGCTC 1 cut(s) 589
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 589
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoO109I RGGNCCY 1 cut(s) 403
EcoRII CCWGG 3 cut(s) 495, 506, 515
EcoT14I CCWWGG 3 cut(s) 297, 318, 447
EcoT38I GRGCYC 1 cut(s) 591
EgeI GGCGCC 1 cut(s) 207
EheI GGCGCC 1 cut(s) 207
ErhI CCWWGG 3 cut(s) 297, 318, 447
FaeI CATG 2 cut(s) 451, 460
FaiI YATR 9 cut(s) 95, 97, 99, 118, 449, 458, 712, 719, 721
FaqI GGGAC 1 cut(s) 628
FatI CATG 2 cut(s) 447, 456
FauI CCCGC 1 cut(s) 289
Fnu4HI GCNGC 3 cut(s) 69, 333, 336
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 3 cut(s) 69, 333, 336
FspBI CTAG 1 cut(s) 491
GlaI GCGC 1 cut(s) 207
GluI GCNGC 3 cut(s) 69, 333, 336
GsaI CCCAGC 1 cut(s) 68
GsuI CTGGAG 1 cut(s) 421
HaeII RGCGCY 1 cut(s) 209
HaeIII GGCC 4 cut(s) 302, 332, 446, 623
HapII CCGG 4 cut(s) 303, 327, 339, 582
HhaI GCGC 1 cut(s) 208
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 2 cut(s) 451, 460
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HinfI GANTC 4 cut(s) 78, 432, 453, 500
HpaII CCGG 4 cut(s) 303, 327, 339, 582
HphI GGTGA 2 cut(s) 30, 82
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 2 cut(s) 431, 610
Hpy188III TCNNGA 6 cut(s) 56, 406, 457, 484, 548, 556
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 1 cut(s) 605
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 2 cut(s) 4, 636
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 275, 332, 595, 599
HpyF3I CTNAG 2 cut(s) 148, 630
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 2 cut(s) 451, 460
HspAI GCGC 1 cut(s) 206
KasI GGCGCC 1 cut(s) 205
KroI GCCGGC 1 cut(s) 338
KroNI GCCGGC 1 cut(s) 340
Kzo9I GATC 6 cut(s) 42, 49, 359, 387, 552, 681
LmnI GCTCC 1 cut(s) 318
Lsp1109I GCAGC 1 cut(s) 80
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 625, 656
MalI GATC 6 cut(s) 44, 51, 361, 389, 554, 683
MbiI CCGCTC 1 cut(s) 88
MboI GATC 6 cut(s) 42, 49, 359, 387, 552, 681
MflI RGATCY 2 cut(s) 387, 552
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 1 cut(s) 23
Mly113I GGCGCC 1 cut(s) 206
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 2 cut(s) 106, 166
MnlI CCTC 4 cut(s) 63, 124, 241, 561
MreI CGCCGGCG 1 cut(s) 338
MroNI GCCGGC 1 cut(s) 338
MroXI GAANNNNTTC 1 cut(s) 464
MseI TTAA 1 cut(s) 32
MspI CCGG 4 cut(s) 303, 327, 339, 582
MspR9I CCNGG 6 cut(s) 304, 328, 497, 508, 517, 582
MvaI CCWGG 3 cut(s) 497, 508, 517
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 5 cut(s) 212, 275, 332, 595, 599
NaeI GCCGGC 1 cut(s) 340
NarI GGCGCC 1 cut(s) 206
NciI CCSGG 3 cut(s) 304, 328, 582
NcoI CCATGG 1 cut(s) 447
NdeII GATC 6 cut(s) 42, 49, 359, 387, 552, 681
NgoMIV GCCGGC 1 cut(s) 338
NheI GCTAGC 1 cut(s) 490
NlaIII CATG 2 cut(s) 451, 460
NlaIV GGNNCC 2 cut(s) 207, 331
NmeAIII GCCGAG 2 cut(s) 241, 310
NmuCI GTSAC 2 cut(s) 625, 656
PagI TCATGA 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PdiI GCCGGC 1 cut(s) 340
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 3 cut(s) 78, 432, 453
PkrI GCNGC 3 cut(s) 70, 334, 337
PleI GAGTC 1 cut(s) 508
PluTI GGCGCC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 508
PpuMI RGGWCCY 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 3 cut(s) 495, 506, 515
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 3 cut(s) 495, 506, 515
PspN4I GGNNCC 2 cut(s) 207, 331
PspPI GGNCC 3 cut(s) 330, 403, 415
PspPPI RGGWCCY 1 cut(s) 403
PsuI RGATCY 2 cut(s) 387, 552
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
SacI GAGCTC 1 cut(s) 591
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 3 cut(s) 69, 333, 336
Sau3AI GATC 6 cut(s) 42, 49, 359, 387, 552, 681
Sau96I GGNCC 3 cut(s) 330, 403, 415
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 6 cut(s) 304, 328, 497, 508, 517, 582
SduI GDGCHC 1 cut(s) 591
SetI ASST 9 cut(s) 315, 375, 383, 405, 417, 467, 492, 521, 591
SfoI GGCGCC 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 338
SinI GGWCC 2 cut(s) 403, 415
Sse9I AATT 1 cut(s) 23
SsiI CCGC 8 cut(s) 46, 86, 230, 269, 282, 333, 336, 561
SspDI GGCGCC 1 cut(s) 205
SspMI CTAG 1 cut(s) 491
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 6 cut(s) 302, 326, 495, 506, 515, 580
StyI CCWWGG 3 cut(s) 297, 318, 447
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 375
TaqI TCGA 4 cut(s) 165, 362, 483, 680
TaqII GACCGA 1 cut(s) 113
TasI AATT 1 cut(s) 23
TauI GCSGC 2 cut(s) 335, 338
TfiI GAWTC 3 cut(s) 78, 432, 453
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseFI GTSAC 2 cut(s) 625, 656
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 2 cut(s) 625, 656
TspDTI ATGAA 4 cut(s) 457, 473, 706, 717
VpaK11BI GGWCC 2 cut(s) 403, 415
XagI CCTNNNNNAGG 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.