pycom11g11640

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Forward (+)
10354472 .. 10358969
4498 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
ATGGCGAGTACTTTAGGGTTAATGTTGATTTCTCTTTCCGTCATCTTAATCAATATCATCTCCCTTCCTCTCCCATCAATGGCTATTTCTTCATGCAATGGCCCATGCAAAACTCCCGACGGCTGTACTGGCAAGCTCACGTGCATTAACCGCAAGTGCAACGATGACCCCGATGTTGGAACCCACATATGCGGTGGAGGCGGAGGAGGAGGCAGTTGTAAGTCGTCAGGAACTCTCAATTACAAGCATCCACGAGCGCCAAACTCACGCTTAACGATTTCAGTGAAGGCGGGGGCACCTCAGAGTGTGATGGGAAATTCCAACCGAACAGTGAGCGCGTGGTGGCACTTTCCACCGGGTGGTATGATATGGTCGAGATGCGGAAGGTTGATAAGAATTACGCTTAGTAATGGGAGGAGTGTGACGGCAAAAGTGGTGGACGAGTGTGATTCGGTAAATGGATGTGACAGTGAGCATGCTGGTCAACCACCGTGTAAAAACAACATTGTTGATGGCTCTGCAGCTGTTTGGAATGTTTTGGGATTGAACCAAGATTTAGAAATCGTGGACGTGACTTGGTCCTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

20.46

Weight (kDa)

8.31

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 125 - 194 2.5e-24 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AccB7I CCANNNNNTGG 1 cut(s) 359
AccII CGCG 1 cut(s) 338
AciI CCGC 5 cut(s) 151, 192, 201, 290, 381
AcsI RAATTY 1 cut(s) 316
AcvI CACGTG 1 cut(s) 141
AdeI CACNNNGTG 1 cut(s) 359
AfaI GTAC 2 cut(s) 10, 127
AfiI CCNNNNNNNGG 3 cut(s) 79, 176, 359
AgsI TTSAA 1 cut(s) 547
AjiI CACGTC 1 cut(s) 571
AluBI AGCT 2 cut(s) 136, 524
AluI AGCT 2 cut(s) 136, 524
AoxI GGCC 2 cut(s) 100, 585
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 1 cut(s) 316
AspLEI GCGC 2 cut(s) 259, 338
AspS9I GGNCC 2 cut(s) 101, 579
AsuC2I CCSGG 1 cut(s) 357
AvaII GGWCC 1 cut(s) 579
BaeGI GKGCMC 1 cut(s) 298
BanI GGYRCC 1 cut(s) 295
BauI CACGAG 1 cut(s) 252
BbrPI CACGTG 1 cut(s) 141
BbvI GCAGC 1 cut(s) 533
BccI CCATC 3 cut(s) 82, 304, 506
BceAI ACGGC 2 cut(s) 136, 441
BcnI CCSGG 1 cut(s) 357
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 1 cut(s) 260
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 1 cut(s) 579
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 2 cut(s) 101, 579
BmiI GGNNCC 2 cut(s) 181, 297
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 2 cut(s) 256, 368
BpuMI CCSGG 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 582
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 176, 359
Bse1I ACTGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 2 cut(s) 247, 467
BseLI CCNNNNNNNGG 3 cut(s) 79, 176, 359
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 3 cut(s) 219, 222, 430
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 2 cut(s) 102, 587
BshNI GGYRCC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 356
BslI CCNNNNNNNGG 3 cut(s) 79, 176, 359
BsnI GGCC 2 cut(s) 102, 587
Bsp1286I GDGCHC 1 cut(s) 298
BspACI CCGC 5 cut(s) 151, 192, 201, 290, 381
BspANI GGCC 2 cut(s) 102, 587
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 181, 297
BspMAI CTGCAG 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 582
BssSI CACGAG 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 331, 470, 492
BstBAI YACGTR 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 134, 477
BstDEI CTNAG 2 cut(s) 300, 404
BstF5I GGATG 2 cut(s) 247, 467
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 260
BstHHI GCGC 2 cut(s) 259, 338
BstMWI GCNNNNNNNGC 3 cut(s) 129, 150, 198
BstNSI RCATGY 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 533
BsuRI GGCC 2 cut(s) 102, 587
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 2 cut(s) 247, 467
BtsIMutI CAGTG 3 cut(s) 288, 336, 475
Cac8I GCNNGC 2 cut(s) 134, 477
CfoI GCGC 2 cut(s) 259, 338
Cfr13I GGNCC 2 cut(s) 101, 579
Csp6I GTAC 2 cut(s) 9, 126
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 3 cut(s) 93, 105, 476
CviJI RGCY 7 cut(s) 83, 102, 123, 136, 516, 524, 587
CviKI_1 RGCY 7 cut(s) 83, 102, 123, 136, 516, 524, 587
CviQI GTAC 2 cut(s) 9, 126
DdeI CTNAG 2 cut(s) 300, 404
DraIII CACNNNGTG 1 cut(s) 359
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 579
Eco72I CACGTG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 3 cut(s) 96, 108, 479
FaiI YATR 7 cut(s) 94, 106, 188, 190, 365, 370, 477
FatI CATG 3 cut(s) 92, 104, 475
FauI CCCGC 1 cut(s) 283
FauNDI CATATG 1 cut(s) 188
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 2 cut(s) 234, 474
Fsp4HI GCNGC 1 cut(s) 522
GlaI GCGC 2 cut(s) 258, 337
GluI GCNGC 1 cut(s) 522
HaeII RGCGCY 1 cut(s) 260
HaeIII GGCC 2 cut(s) 102, 587
HapII CCGG 1 cut(s) 356
HhaI GCGC 2 cut(s) 259, 338
Hin1II CATG 3 cut(s) 96, 108, 479
Hin6I GCGC 2 cut(s) 257, 336
HinP1I GCGC 2 cut(s) 257, 336
HincII GTYRAC 1 cut(s) 485
HindII GTYRAC 1 cut(s) 485
HinfI GANTC 1 cut(s) 449
HpaII CCGG 1 cut(s) 356
Hpy166II GTNNAC 3 cut(s) 439, 485, 568
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 3 cut(s) 116, 228, 375
Hpy8I GTNNAC 3 cut(s) 439, 485, 568
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 74, 280, 378
HpyCH4III ACNGT 3 cut(s) 331, 470, 492
HpyCH4IV ACGT 2 cut(s) 140, 570
HpyCH4V TGCA 5 cut(s) 96, 108, 144, 159, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 150, 198
HpyF3I CTNAG 2 cut(s) 300, 404
HpySE526I ACGT 2 cut(s) 140, 570
Hsp92II CATG 3 cut(s) 96, 108, 479
HspAI GCGC 2 cut(s) 257, 336
LpnPI CCDG 4 cut(s) 114, 213, 369, 465
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 2 cut(s) 256, 368
MaeII ACGT 2 cut(s) 140, 570
MaeIII GTNAC 3 cut(s) 421, 464, 571
MboII GAAGA 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 3 cut(s) 238, 316, 396
MmeI TCCRAC 2 cut(s) 157, 345
MnlI CCTC 7 cut(s) 78, 191, 197, 200, 203, 309, 408
MseI TTAA 4 cut(s) 20, 47, 147, 272
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 129, 150, 198
NciI CCSGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 188
NlaIII CATG 3 cut(s) 96, 108, 479
NlaIV GGNNCC 2 cut(s) 181, 297
NmuCI GTSAC 3 cut(s) 421, 464, 571
NspI RCATGY 1 cut(s) 479
PaeI GCATGC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PfeI GAWTC 1 cut(s) 449
PflFI GACNNNGTC 1 cut(s) 577
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 1 cut(s) 523
PmaCI CACGTG 1 cut(s) 141
PmlI CACGTG 1 cut(s) 141
Ppu21I YACGTR 1 cut(s) 141
PspCI CACGTG 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 181, 297
PspPI GGNCC 2 cut(s) 101, 579
PstI CTGCAG 1 cut(s) 523
PsyI GACNNNGTC 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 2 cut(s) 10, 127
RsaNI GTAC 2 cut(s) 9, 126
SaqAI TTAA 4 cut(s) 20, 47, 147, 272
SatI GCNGC 1 cut(s) 522
Sau96I GGNCC 2 cut(s) 101, 579
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 1 cut(s) 298
SetI ASST 6 cut(s) 138, 143, 301, 389, 526, 573
SfaNI GCATC 2 cut(s) 256, 368
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 1 cut(s) 579
SphI GCATGC 1 cut(s) 479
Sse9I AATT 3 cut(s) 238, 316, 396
SsiI CCGC 5 cut(s) 151, 192, 201, 290, 381
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 3 cut(s) 331, 470, 492
TaiI ACGT 2 cut(s) 143, 573
TaqI TCGA 1 cut(s) 374
TasI AATT 3 cut(s) 238, 316, 396
TatI WGTACW 2 cut(s) 8, 125
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 4 cut(s) 20, 47, 147, 272
Tru9I TTAA 4 cut(s) 20, 47, 147, 272
TscAI CASTG 3 cut(s) 288, 336, 475
TseFI GTSAC 3 cut(s) 421, 464, 571
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 3 cut(s) 421, 464, 571
TspDTI ATGAA 1 cut(s) 81
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 3 cut(s) 288, 336, 475
Tth111I GACNNNGTC 1 cut(s) 577
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 579
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 479
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.