pycom11g19850

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Reverse (-)
22382859 .. 22384241
1383 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 324 bp
ATGAACCACGACACACAGTCGTGCATGGACCCCAACGTCATGGAGGCCAAGGTTGTGGTGAGCTCATGCGGCCATGATGGACCCATGGGAGCCACCGGGGTGAAGAGGTTGAGGAGTGTCGGAATGATTGAGAGCGTTCCTGGGATGAAGGCATTGGACATGAACACAGCTGAGGATGCTATTGTGAAGCTCACAAGGGAGATTGTGCCTGGCATGATCGTTACCGGCATGGAAGTTGCTGAGATTGATGGATCTCCAAGAATGGGGCCTACATTCGGAGCCATGATGATCGATCAGGGCAAAAGGCAGCTCACTTGGCATTGA

Protein Analysis

108

Amino Acids

11.49

Weight (kDa)

5.49

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 1 - 92 6.8e-40 Thi4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
AciI CCGC 1 cut(s) 69
AclWI GGATC 1 cut(s) 259
AcoI YGGCCR 1 cut(s) 70
AfiI CCNNNNNNNGG 2 cut(s) 263, 275
AhdI GACNNNNNGTC 1 cut(s) 16
AjnI CCWGG 2 cut(s) 139, 208
AleI CACNNNNGTG 2 cut(s) 19, 98
AluBI AGCT 4 cut(s) 63, 170, 190, 310
AluI AGCT 4 cut(s) 63, 170, 190, 310
Alw21I GWGCWC 1 cut(s) 65
AlwI GGATC 1 cut(s) 259
AoxI GGCC 3 cut(s) 45, 70, 266
ApeKI GCWGC 1 cut(s) 307
AspS9I GGNCC 3 cut(s) 28, 80, 266
AsuC2I CCSGG 1 cut(s) 97
AsuHPI GGTGA 2 cut(s) 70, 112
AvaII GGWCC 2 cut(s) 28, 80
BanII GRGCYC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 65
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 319
BccI CCATC 2 cut(s) 71, 242
BciT130I CCWGG 2 cut(s) 141, 210
BcnI CCSGG 1 cut(s) 97
BisI GCNGC 2 cut(s) 70, 308
BlsI GCNGC 2 cut(s) 71, 309
Bme1390I CCNGG 3 cut(s) 97, 141, 210
Bme18I GGWCC 2 cut(s) 28, 80
BmeRI GACNNNNNGTC 1 cut(s) 16
BmgT120I GGNCC 3 cut(s) 28, 80, 266
BmiI GGNNCC 5 cut(s) 30, 82, 91, 267, 280
BmrFI CCNGG 3 cut(s) 97, 141, 210
BmsI GCATC 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 97
Bsa29I ATCGAT 1 cut(s) 291
BsaJI CCNNGG 4 cut(s) 48, 84, 96, 140
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 275
Bse118I RCCGGY 1 cut(s) 224
BseBI CCWGG 2 cut(s) 141, 210
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 4 cut(s) 48, 84, 96, 140
BseGI GGATG 2 cut(s) 150, 181
BseLI CCNNNNNNNGG 2 cut(s) 263, 275
BseMII CTCAG 2 cut(s) 162, 231
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 1 cut(s) 319
BshFI GGCC 3 cut(s) 47, 72, 268
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 96, 225
BslI CCNNNNNNNGG 2 cut(s) 263, 275
BsnI GGCC 3 cut(s) 47, 72, 268
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 4 cut(s) 216, 251, 288, 292
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 1 cut(s) 69
BspANI GGCC 3 cut(s) 47, 72, 268
BspCNI CTCAG 2 cut(s) 163, 232
BspDI ATCGAT 1 cut(s) 291
BspLI GGNNCC 5 cut(s) 30, 82, 91, 267, 280
BspPI GGATC 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 224
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 4 cut(s) 48, 84, 96, 140
BssMI GATC 4 cut(s) 216, 251, 288, 292
BssT1I CCWWGG 2 cut(s) 48, 84
Bst2UI CCWGG 2 cut(s) 141, 210
Bst4CI ACNGT 1 cut(s) 18
Bst6I CTCTTC 1 cut(s) 98
BstDEI CTNAG 2 cut(s) 171, 240
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 150, 181
BstKTI GATC 4 cut(s) 219, 254, 291, 295
BstMBI GATC 4 cut(s) 216, 251, 288, 292
BstMWI GCNNNNNNNGC 3 cut(s) 69, 176, 316
BstNI CCWGG 2 cut(s) 141, 210
BstSCI CCNGG 3 cut(s) 95, 139, 208
BstV1I GCAGC 1 cut(s) 319
BstX2I RGATCY 1 cut(s) 251
BstXI CCANNNNNNTGG 2 cut(s) 40, 55
BstYI RGATCY 1 cut(s) 251
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 3 cut(s) 47, 72, 268
BsuTUI ATCGAT 1 cut(s) 291
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 2 cut(s) 150, 181
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 28, 80, 266
ClaI ATCGAT 1 cut(s) 291
CviAII CATG 9 cut(s) 25, 40, 66, 74, 85, 160, 214, 229, 283
CviJI RGCY 9 cut(s) 47, 63, 72, 92, 170, 190, 268, 281, 310
CviKI_1 RGCY 9 cut(s) 47, 63, 72, 92, 170, 190, 268, 281, 310
DdeI CTNAG 2 cut(s) 171, 240
DpnI GATC 4 cut(s) 218, 253, 290, 294
DpnII GATC 4 cut(s) 216, 251, 288, 292
DrdI GACNNNNNNGTC 1 cut(s) 35
DriI GACNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 98
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 98
Ecl136II GAGCTC 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 48, 84
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 28, 80
Eco53kI GAGCTC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 139, 208
EcoT14I CCWWGG 2 cut(s) 48, 84
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 48, 84
FaeI CATG 9 cut(s) 28, 43, 69, 77, 88, 163, 217, 232, 286
FaiI YATR 9 cut(s) 26, 41, 67, 75, 86, 161, 215, 230, 284
FatI CATG 9 cut(s) 24, 39, 65, 73, 84, 159, 213, 228, 282
Fnu4HI GCNGC 2 cut(s) 70, 308
FokI GGATG 2 cut(s) 157, 188
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 2 cut(s) 70, 308
GluI GCNGC 2 cut(s) 70, 308
HaeIII GGCC 3 cut(s) 47, 72, 268
HapII CCGG 2 cut(s) 96, 225
Hin1II CATG 9 cut(s) 28, 43, 69, 77, 88, 163, 217, 232, 286
HpaII CCGG 2 cut(s) 96, 225
HphI GGTGA 2 cut(s) 70, 112
Hpy188I TCNGA 2 cut(s) 122, 278
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 18
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 176, 316
HpyF3I CTNAG 2 cut(s) 171, 240
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 9 cut(s) 28, 43, 69, 77, 88, 163, 217, 232, 286
Kzo9I GATC 4 cut(s) 216, 251, 288, 292
LmnI GCTCC 2 cut(s) 89, 278
LpnPI CCDG 7 cut(s) 109, 126, 153, 195, 222, 238, 281
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 1 cut(s) 166
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 220
MalI GATC 4 cut(s) 218, 253, 290, 294
MboI GATC 4 cut(s) 216, 251, 288, 292
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 4 cut(s) 37, 99, 105, 166
MslI CAYNNNNRTG 2 cut(s) 19, 98
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 96, 225
MspR9I CCNGG 3 cut(s) 97, 141, 210
MvaI CCWGG 2 cut(s) 141, 210
MwoI GCNNNNNNNGC 3 cut(s) 69, 176, 316
NciI CCSGG 1 cut(s) 97
NcoI CCATGG 1 cut(s) 84
NdeII GATC 4 cut(s) 216, 251, 288, 292
NlaIII CATG 9 cut(s) 28, 43, 69, 77, 88, 163, 217, 232, 286
NlaIV GGNNCC 5 cut(s) 30, 82, 91, 267, 280
OliI CACNNNNGTG 2 cut(s) 19, 98
PkrI GCNGC 2 cut(s) 71, 309
Psp124BI GAGCTC 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 139, 208
PspGI CCWGG 2 cut(s) 139, 208
PspN4I GGNNCC 5 cut(s) 30, 82, 91, 267, 280
PspPI GGNCC 3 cut(s) 28, 80, 266
PsuI RGATCY 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 170
RseI CAYNNNNRTG 2 cut(s) 19, 98
SacI GAGCTC 1 cut(s) 65
SatI GCNGC 2 cut(s) 70, 308
Sau3AI GATC 4 cut(s) 216, 251, 288, 292
Sau96I GGNCC 3 cut(s) 28, 80, 266
ScrFI CCNGG 3 cut(s) 97, 141, 210
SduI GDGCHC 1 cut(s) 65
SetI ASST 7 cut(s) 39, 54, 65, 110, 172, 192, 312
SfaNI GCATC 1 cut(s) 166
SinI GGWCC 2 cut(s) 28, 80
SmiMI CAYNNNNRTG 2 cut(s) 19, 98
SsiI CCGC 1 cut(s) 69
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 3 cut(s) 95, 139, 208
StyI CCWWGG 2 cut(s) 48, 84
TaaI ACNGT 1 cut(s) 18
TaiI ACGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 291
TauI GCSGC 1 cut(s) 72
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 17, 161, 176
VpaK11BI GGWCC 2 cut(s) 28, 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.