pycom11g24880

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Forward (+)
27712124 .. 27712798
675 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGCGCCGGTGTGTGGCTTGGACCGGAGCTATAAGAAGAAATGAATGTTCAGCCAAGGATCCGGCTGTATACGTGTTCCGACACAAACCTCCCCGAATTCTTGGCCTTCGACCTCAACTCTCCCACCCAAACCCCATCCTCGGACTCAATCTCGAACATCTGTCCTATTGTAATCGGCATCACAATTGCAAAGGTCAGCCGTCGGCGGTGGAGGTGGTGCATGTGATGTACAGCGATTTTGAAGGCACTTTTGCGACGGAGCCTACGACGCCAATGACACCATCTACACCAGAAACTAGCGCTCCAACGATACCAATCCTACCACTGCCATCGGTACAGGTAATGCCACTGCCGTCGCCACCACCTTCACTGCCATCAATCACCCCAGATATCATGCCACCGTCGCTACCACCATTGCCAATCATGCCACCGCTGCCACCACCAGTGCAAATCACACCACTGTCGCAATCAATCACGCCACCACAGCCAACTAAACCACCTGTGCTACCGCTACCATGTTTCATTCGTGACACTAGAATGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

182

Amino Acids

19.85

Weight (kDa)

8.97

Isoelectric Point (pI)

74.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AciI CCGC 3 cut(s) 206, 431, 509
AclWI GGATC 2 cut(s) 53, 66
AcsI RAATTY 1 cut(s) 96
AcyI GRCGYC 1 cut(s) 269
AfaI GTAC 2 cut(s) 230, 336
AfeI AGCGCT 1 cut(s) 301
AfiI CCNNNNNNNGG 2 cut(s) 13, 140
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 1 cut(s) 242
AluBI AGCT 1 cut(s) 29
AluI AGCT 1 cut(s) 29
AlwI GGATC 2 cut(s) 53, 66
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 433
ApoI RAATTY 1 cut(s) 96
AspLEI GCGC 2 cut(s) 6, 302
AspS9I GGNCC 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 373
AvaII GGWCC 1 cut(s) 21
BamHI GGATCC 1 cut(s) 58
BbvI GCAGC 1 cut(s) 420
BccI CCATC 4 cut(s) 143, 289, 337, 382
BceAI ACGGC 2 cut(s) 184, 337
BfaI CTAG 2 cut(s) 297, 534
BfoI RGCGCY 1 cut(s) 303
BisI GCNGC 1 cut(s) 434
BlsI GCNGC 1 cut(s) 435
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 2 cut(s) 60, 261
BmsI GCATC 1 cut(s) 187
BsaAI YACGTR 1 cut(s) 73
BsaBI GATNNNNATC 1 cut(s) 314
BsaHI GRCGYC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 54, 139
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 140
Bse118I RCCGGY 1 cut(s) 6
Bse1I ACTGG 1 cut(s) 443
Bse3DI GCAATG 1 cut(s) 413
Bse8I GATNNNNATC 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 54, 139
BseGI GGATG 1 cut(s) 135
BseJI GATNNNNATC 1 cut(s) 314
BseLI CCNNNNNNNGG 2 cut(s) 13, 140
BseMI GCAATG 1 cut(s) 413
BseNI ACTGG 1 cut(s) 443
BseXI GCAGC 1 cut(s) 420
BshFI GGCC 1 cut(s) 105
BsiSI CCGG 3 cut(s) 7, 24, 62
BslI CCNNNNNNNGG 2 cut(s) 13, 140
BsnI GGCC 1 cut(s) 105
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 1 cut(s) 58
BspACI CCGC 3 cut(s) 206, 431, 509
BspANI GGCC 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 60, 261
BspPI GGATC 2 cut(s) 53, 66
BsrDI GCAATG 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 1 cut(s) 6
BssECI CCNNGG 2 cut(s) 54, 139
BssMI GATC 1 cut(s) 58
BssNAI GTATAC 1 cut(s) 70
BssNI GRCGYC 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 54
Bst1107I GTATAC 1 cut(s) 70
Bst4CI ACNGT 2 cut(s) 402, 462
BstACI GRCGYC 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 228
BstBAI YACGTR 1 cut(s) 73
BstF5I GGATG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 2 cut(s) 6, 302
BstKTI GATC 1 cut(s) 61
BstMBI GATC 1 cut(s) 58
BstMWI GCNNNNNNNGC 5 cut(s) 268, 403, 424, 433, 484
BstNSI RCATGY 1 cut(s) 224
BstV1I GCAGC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BstZ17I GTATAC 1 cut(s) 70
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 1 cut(s) 135
BtsI GCAGTG 3 cut(s) 323, 347, 368
BtsIMutI CAGTG 5 cut(s) 323, 347, 368, 450, 458
CfoI GCGC 2 cut(s) 6, 302
Cfr10I RCCGGY 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 21
CseI GACGC 1 cut(s) 277
Csp6I GTAC 2 cut(s) 229, 335
CviAII CATG 4 cut(s) 221, 394, 424, 516
CviJI RGCY 8 cut(s) 17, 29, 53, 65, 105, 199, 262, 487
CviKI_1 RGCY 8 cut(s) 17, 29, 53, 65, 105, 199, 262, 487
CviQI GTAC 2 cut(s) 229, 335
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 54
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 21
Eco47III AGCGCT 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 96
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 224, 397, 427, 519
FaiI YATR 6 cut(s) 32, 70, 222, 395, 425, 517
FatI CATG 4 cut(s) 220, 393, 423, 515
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 434
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 1 cut(s) 434
FspBI CTAG 2 cut(s) 297, 534
GlaI GCGC 2 cut(s) 5, 301
GluI GCNGC 1 cut(s) 434
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 1 cut(s) 105
HapII CCGG 3 cut(s) 7, 24, 62
HgaI GACGC 1 cut(s) 277
HhaI GCGC 2 cut(s) 6, 302
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 4 cut(s) 224, 397, 427, 519
Hin6I GCGC 2 cut(s) 4, 300
HinP1I GCGC 2 cut(s) 4, 300
HinfI GANTC 1 cut(s) 144
HpaII CCGG 3 cut(s) 7, 24, 62
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 80, 143
Hpy188III TCNNGA 2 cut(s) 152, 527
Hpy8I GTNNAC 1 cut(s) 70
Hpy99I CGWCG 5 cut(s) 205, 259, 271, 358, 406
HpyAV CCTTC 3 cut(s) 116, 236, 375
HpyCH4III ACNGT 2 cut(s) 402, 462
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 3 cut(s) 189, 220, 448
HpyF10VI GCNNNNNNNGC 5 cut(s) 268, 403, 424, 433, 484
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 4 cut(s) 224, 397, 427, 519
HspAI GCGC 2 cut(s) 4, 300
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 3 cut(s) 26, 259, 307
LpnPI CCDG 8 cut(s) 20, 37, 75, 303, 323, 399, 456, 513
Lsp1109I GCAGC 1 cut(s) 420
LweI GCATC 1 cut(s) 187
MaeI CTAG 2 cut(s) 297, 534
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 527
MalI GATC 1 cut(s) 60
MboI GATC 1 cut(s) 58
MboII GAAGA 1 cut(s) 48
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 58
MluCI AATT 2 cut(s) 96, 184
MlyI GAGTC 1 cut(s) 138
MmeI TCCRAC 2 cut(s) 103, 329
MnlI CCTC 4 cut(s) 99, 123, 149, 205
MslI CAYNNNNRTG 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 433
MspI CCGG 3 cut(s) 7, 24, 62
MunI CAATTG 1 cut(s) 184
MwoI GCNNNNNNNGC 5 cut(s) 268, 403, 424, 433, 484
NdeII GATC 1 cut(s) 58
NlaIII CATG 4 cut(s) 224, 397, 427, 519
NlaIV GGNNCC 2 cut(s) 60, 261
NmuCI GTSAC 1 cut(s) 527
NspI RCATGY 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PkrI GCNGC 1 cut(s) 435
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Ppu21I YACGTR 1 cut(s) 73
PspN4I GGNNCC 2 cut(s) 60, 261
PspPI GGNCC 1 cut(s) 21
PsuI RGATCY 1 cut(s) 58
RsaI GTAC 2 cut(s) 230, 336
RsaNI GTAC 2 cut(s) 229, 335
RseI CAYNNNNRTG 1 cut(s) 536
SatI GCNGC 1 cut(s) 434
Sau3AI GATC 1 cut(s) 58
Sau96I GGNCC 1 cut(s) 21
SchI GAGTC 1 cut(s) 138
SetI ASST 9 cut(s) 31, 75, 91, 115, 196, 216, 342, 367, 502
SfaNI GCATC 1 cut(s) 187
SgrAI CRCCGGYG 1 cut(s) 6
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 536
Sse9I AATT 2 cut(s) 96, 184
SsiI CCGC 3 cut(s) 206, 431, 509
SspMI CTAG 2 cut(s) 297, 534
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 402, 462
TaiI ACGT 1 cut(s) 75
TaqI TCGA 2 cut(s) 109, 153
TasI AATT 2 cut(s) 96, 184
TatI WGTACW 1 cut(s) 228
TscAI CASTG 5 cut(s) 330, 354, 375, 450, 465
TseFI GTSAC 1 cut(s) 527
TseI GCWGC 1 cut(s) 433
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 2 cut(s) 57, 511
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 5 cut(s) 330, 354, 375, 450, 465
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 1 cut(s) 96
XceI RCATGY 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 2 cut(s) 297, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.